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  3. Suc-Ala-Ala-Phe-AMC

Suc-Ala-Ala-Phe-AMC is a fluorogenic chymotrypsin substrate, can be hydrolyzed by endopeptidase. Suc-Ala-Ala-Phe-AMC has been used in both in vivo assays of the acrosome reaction and in vitro enzyme assays.

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Custom Peptide Synthesis

Suc-Ala-Ala-Phe-AMC Chemical Structure

Suc-Ala-Ala-Phe-AMC Chemical Structure

CAS No. : 71973-79-0

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1 mg USD 70 In-stock
5 mg USD 210 In-stock
10 mg USD 340 In-stock
25 mg USD 650 In-stock
50 mg USD 1040 In-stock
100 mg USD 1650 In-stock
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Based on 1 publication(s) in Google Scholar

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Description

Suc-Ala-Ala-Phe-AMC is a fluorogenic chymotrypsin substrate, can be hydrolyzed by endopeptidase. Suc-Ala-Ala-Phe-AMC has been used in both in vivo assays of the acrosome reaction and in vitro enzyme assays[1][2][3].

Molecular Weight

564.59

Formula

C29H32N4O8

CAS No.
Appearance

Solid

Color

White to off-white

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture and light

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (442.80 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.7712 mL 8.8560 mL 17.7120 mL
5 mM 0.3542 mL 1.7712 mL 3.5424 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.7712 mL 8.8560 mL 17.7120 mL 44.2799 mL
5 mM 0.3542 mL 1.7712 mL 3.5424 mL 8.8560 mL
10 mM 0.1771 mL 0.8856 mL 1.7712 mL 4.4280 mL
15 mM 0.1181 mL 0.5904 mL 1.1808 mL 2.9520 mL
20 mM 0.0886 mL 0.4428 mL 0.8856 mL 2.2140 mL
25 mM 0.0708 mL 0.3542 mL 0.7085 mL 1.7712 mL
30 mM 0.0590 mL 0.2952 mL 0.5904 mL 1.4760 mL
40 mM 0.0443 mL 0.2214 mL 0.4428 mL 1.1070 mL
50 mM 0.0354 mL 0.1771 mL 0.3542 mL 0.8856 mL
60 mM 0.0295 mL 0.1476 mL 0.2952 mL 0.7380 mL
80 mM 0.0221 mL 0.1107 mL 0.2214 mL 0.5535 mL
100 mM 0.0177 mL 0.0886 mL 0.1771 mL 0.4428 mL
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Suc-Ala-Ala-Phe-AMC Related Classifications

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Suc-Ala-Ala-Phe-AMC
Cat. No.:
HY-137337
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