Suc-Ala-Ala-Phe-AMC
Based on 1 Customer Validation
Suc-Ala-Ala-Phe-AMC is a fluorogenic chymotrypsin substrate, can be hydrolyzed by endopeptidase. Suc-Ala-Ala-Phe-AMC has been used in both in vivo assays of the acrosome reaction and in vitro enzyme assays.
For research use only. We do not sell to patients.
- Purity: 99.89%
- CAS No.: 71973-79-0
- Formula: C29H32N4O8
- Molecular Weight:564.59
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Storage:
Sealed storage, away from moisture and light.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Biological Activity
Chemical Information
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CAS No. 71973-79-0
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Appearance Solid
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Molecular Weight 564.59
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Formula C29H32N4O8
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Color White to off-white
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture and light
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
DMSO : 250 mg/mL (442.80 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (270 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Farach HA Jr, et al. Evidence for the involvement of metalloendoproteases in the acrosome reaction in sea urchin sperm. J Biol Chem. 1987 Apr 25;262(12):5483-7. [Content Brief]
[2]. Pinto MR, et al. Chymotrypsin-like enzymes are involved in sperm penetration through the vitelline coat of Ciona intestinalis egg. Mol Reprod Dev. 1990 Aug;26(4):319-23. [Content Brief]
[3]. Mumford RA, et al. A zinc metalloendopeptidase associated with dog pancreatic membranes. J Biol Chem. 1980 Mar 25;255(6):2227-30. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.7712 mL | 8.8560 mL | 17.7120 mL | 44.2799 mL |
| 5 mM | 0.3542 mL | 1.7712 mL | 3.5424 mL | 8.8560 mL | |
| 10 mM | 0.1771 mL | 0.8856 mL | 1.7712 mL | 4.4280 mL | |
| 15 mM | 0.1181 mL | 0.5904 mL | 1.1808 mL | 2.9520 mL | |
| 20 mM | 0.0886 mL | 0.4428 mL | 0.8856 mL | 2.2140 mL | |
| 25 mM | 0.0708 mL | 0.3542 mL | 0.7085 mL | 1.7712 mL | |
| 30 mM | 0.0590 mL | 0.2952 mL | 0.5904 mL | 1.4760 mL | |
| 40 mM | 0.0443 mL | 0.2214 mL | 0.4428 mL | 1.1070 mL | |
| 50 mM | 0.0354 mL | 0.1771 mL | 0.3542 mL | 0.8856 mL | |
| 60 mM | 0.0295 mL | 0.1476 mL | 0.2952 mL | 0.7380 mL | |
| 80 mM | 0.0221 mL | 0.1107 mL | 0.2214 mL | 0.5535 mL | |
| 100 mM | 0.0177 mL | 0.0886 mL | 0.1771 mL | 0.4428 mL |