Suc-AAPF-AMC (Synonyms: Suc-Ala-Ala-Pro-Phe-AMC)

Cat. No.: HY-137811 Purity: 98.50%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Suc-AAPF-AMC is an α-chymotrypsin fluorogenic substrate for the quantitative determination of chymotrypsin activity.

For research use only. We do not sell to patients.

Suc-AAPF-AMC Chemical Structure

CAS No. : 88467-45-2

Size	Price	Stock	Quantity
5 mg	USD 75	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 125	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 250	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Purity & Documentation
References
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Description

Suc-AAPF-AMC is an α-chymotrypsin fluorogenic substrate for the quantitative determination of chymotrypsin activity^[1].

Molecular Weight

661.70

Formula

C₃₄H₃₉N₅O₉

CAS No.

88467-45-2

Appearance

Solid

Color

White to off-white

Sequence

{Suc}-Ala-Ala-Pro-Phe-{AMC}

Sequence Shortening

{Suc}-AAPF-{AMC}

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture and light

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (151.13 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.5113 mL	7.5563 mL	15.1126 mL
5 mM	0.3023 mL	1.5113 mL	3.0225 mL
10 mM	0.1511 mL	0.7556 mL	1.5113 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.78 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (3.78 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (3.78 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.50%

Select Batch:

Data Sheet (271 KB) SDS (252 KB)

COA (248 KB) RP-HPLC (301 KB) MS (229 KB)

Handling Instructions (2659 KB)

References

[1]. Urja S Lathia, et al. Development of inductively coupled plasma-mass spectrometry-based protease assays. Anal Biochem. 2010 Mar 1;398(1):93-8. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.5113 mL	7.5563 mL	15.1126 mL	37.7815 mL
	5 mM	0.3023 mL	1.5113 mL	3.0225 mL	7.5563 mL
	10 mM	0.1511 mL	0.7556 mL	1.5113 mL	3.7781 mL
	15 mM	0.1008 mL	0.5038 mL	1.0075 mL	2.5188 mL
	20 mM	0.0756 mL	0.3778 mL	0.7556 mL	1.8891 mL
	25 mM	0.0605 mL	0.3023 mL	0.6045 mL	1.5113 mL
	30 mM	0.0504 mL	0.2519 mL	0.5038 mL	1.2594 mL
	40 mM	0.0378 mL	0.1889 mL	0.3778 mL	0.9445 mL
	50 mM	0.0302 mL	0.1511 mL	0.3023 mL	0.7556 mL
	60 mM	0.0252 mL	0.1259 mL	0.2519 mL	0.6297 mL
	80 mM	0.0189 mL	0.0945 mL	0.1889 mL	0.4723 mL
	100 mM	0.0151 mL	0.0756 mL	0.1511 mL	0.3778 mL

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Suc-AAPF-AMC88467-45-2Suc-Ala-Ala-Pro-Phe-AMCOthersα-chymotrypsinchymotrypsinInhibitorinhibitorinhibit

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