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  4. GFP11

GFP11 is a protein fusion tag consisting of 16 amino acids. When GFP11 and GFP1-10 are sufficiently close in physical space, they undergo spontaneous, irreversible complementation to reassemble into an intact, functional GFP protein that emits green fluorescence. GFP11 can be used to form a split GFP system for high-throughput biotechnology and flow cytometry applications.

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Custom Peptide Synthesis

GFP11

GFP11 Chemical Structure

CAS No. : 862973-56-6

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GFP11 Related Antibodies

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Description

GFP11 is a protein fusion tag consisting of 16 amino acids. When GFP11 and GFP1-10 are sufficiently close in physical space, they undergo spontaneous, irreversible complementation to reassemble into an intact, functional GFP protein that emits green fluorescence. GFP11 can be used to form a split GFP system for high-throughput biotechnology and flow cytometry applications[1][2].

In Vitro

GFP11 can deliver proteins to the outer membrane of Gram-negative bacteria, and enables them to emit green fluorescence via complementation with exogenously added GFP1-10. Due to its low molecular weight, GFP11 does not significantly affect protein solubility and expression, nor does it alter the catalytic activity of the enzyme CsBglA[1].
With its extremely short DNA sequence, GFP11 can be efficiently and inexpensively integrated into endogenous gene loci via CRISPR/Cas9-mediated homology-directed repair, thereby enabling fluorescent labeling of endogenous proteins and providing a technical basis for constructing protein localization libraries[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

GFP11 Related Antibodies
Molecular Weight

1826.04

Formula

C79H124N24O24S
CAS No.
Appearance

Solid

Color

White to off-white

Sequence

Arg-Asp-His-Met-Val-Leu-His-Glu-Tyr-Val-Asn-Ala-Ala-Gly-Ile-Thr
Sequence Shortening

RDHMVLHEYVNAAGIT
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (13.69 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.5476 mL 2.7382 mL 5.4763 mL
5 mM 0.1095 mL 0.5476 mL 1.0953 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 0.5476 mL 2.7382 mL 5.4763 mL 13.6908 mL
5 mM 0.1095 mL 0.5476 mL 1.0953 mL 2.7382 mL
10 mM 0.0548 mL 0.2738 mL 0.5476 mL 1.3691 mL
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GFP11 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

GFP11862973-56-6GFP 11GFP-11Fluorescent DyeIFT20E. coli UT5600 cellsK562 cellsHeLa cellsCRISPR-mediated homology-directed repairHEK293FT cellsmouse IMCD3 cellsgram-negative bacterial surface proteinsGFP1-10Drosophila S2 cellsInhibitorinhibitorinhibit

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