GFP11
Based on 1 Customer Validation
GFP11 is a protein fusion tag consisting of 16 amino acids. When GFP11 and GFP1-10 are sufficiently close in physical space, they undergo spontaneous, irreversible complementation to reassemble into an intact, functional GFP protein that emits green fluorescence. GFP11 can be used to form a split GFP system for high-throughput biotechnology and flow cytometry applications.
For research use only. We do not sell to patients.
- Purity: 98.57%
- CAS No.: 862973-56-6
- Formula: C79H124N24O24S
- Molecular Weight:1826.04
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
GFP11 can deliver proteins to the outer membrane of Gram-negative bacteria, and enables them to emit green fluorescence via complementation with exogenously added GFP1-10. Due to its low molecular weight, GFP11 does not significantly affect protein solubility and expression, nor does it alter the catalytic activity of the enzyme CsBglA[1].
With its extremely short DNA sequence, GFP11 can be efficiently and inexpensively integrated into endogenous gene loci via CRISPR/Cas9-mediated homology-directed repair, thereby enabling fluorescent labeling of endogenous proteins and providing a technical basis for constructing protein localization libraries[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 862973-56-6
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Appearance Solid
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Molecular Weight 1826.04
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Formula C79H124N24O24S
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Color White to off-white
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Sequence
Arg-Asp-His-Met-Val-Leu-His-Glu-Tyr-Val-Asn-Ala-Ala-Gly-Ile-Thr
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Sequence Shortening
RDHMVLHEYVNAAGIT
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 25 mg/mL (13.69 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (272 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Gercke D, et al. Split-GFP complementation at the bacterial cell surface for antibody-free labeling and quantification of heterologous protein display. Enzyme Microb Technol. 2024;174:110391. [Content Brief]
[2]. Kamiyama D, et al. Versatile protein tagging in cells with split fluorescent protein. Nat Commun. 2016;7:11046. Published 2016 Mar 18. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 0.5476 mL | 2.7382 mL | 5.4763 mL | 13.6908 mL |
| 5 mM | 0.1095 mL | 0.5476 mL | 1.0953 mL | 2.7382 mL | |
| 10 mM | 0.0548 mL | 0.2738 mL | 0.5476 mL | 1.3691 mL |
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