フェニレフリン (Synonyms: Phenylephrine; (R)-(-)-Phenylephrine; L-Phenylephrine)

Name: Phenylephrine
Brand: MedChemExpress
SKU: HY-B0769
Rating: 5.0 (34 reviews)

製品番号: HY-B0769 純度: 99.98%: Data Sheet SDS COA 取扱説明書
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(R)-(-)-Phenylephrine is a selective α₁-adrenoceptor agonist primarily used as a decongestant.

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CAS 番号 : 59-42-7

容量	価格（税別）	在庫状況	数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 55	在庫あり	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 55	在庫あり	Estimated Time of Arrival: December 31
Solid
500 mg	$50	在庫あり	Estimated Time of Arrival: December 31
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Based on 34 publication(s) in Google Scholar

Other Forms of Phenylephrine:

顧客検証

Others

Cell Imaging/Staining

RT-PCR

: Phenylephrine purchased from MedChemExpress. Usage Cited in: Trends Biotechnol. 2025 Apr;43(4):918-945. [Abstract]; Cross-sections of samples fabricated change of double- and triple-layered arterial models following Phenylephrine (4 μM) and Acetylcholine treatment.

: Phenylephrine purchased from MedChemExpress. Usage Cited in: J Adv Res. 2025 Oct 29:S2090-1232(25)00848-3. [Abstract]; si-NC or si-FGF21 were infected with adenovirus encoding LC3 for 24 h, then subjected to Phenylephrine (100 μM) stimulation for 24 h. These cells were stained using MitoTracker Red dye, scale bar = 20 µm.

: Phenylephrine purchased from MedChemExpress. Usage Cited in: J Adv Res. 2025 Oct 29:S2090-1232(25)00848-3. [Abstract]; Neonatal rat cardiomyocytes were infected with si-NC or si-PINK1, and then treated with control or 100 μM Phenylephrine for 24 h, followed by vehicle or FGF21 protein. LC3 and PINK1, FGF21 protein levels.

: Phenylephrine purchased from MedChemExpress. Usage Cited in: Mater Des. 2025 Aug.; AC16 and NRVMs were treated with DMSO or Phenylephrine hydrochloride (PE; 100 μM) for 48 h to induce cardiac hypertrophy. Upper panel: the expression of MYH7, BNP and METTL3 were detected by immunoblotting. Lower panel: The densities of the signals were plotted as the mean ± SD from 3 independent experiments.

: Phenylephrine purchased from MedChemExpress. Usage Cited in: Biochem Pharmacol. 2025 Aug 8;242(Pt 1):117218. [Abstract]; RT-qPCR was used to analyze the expression levels of ANP, BNP, β-MHC in H9c2 cells Phenylephrine stimulation.

: Phenylephrine purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Jul 16;15(1):5985. [Abstract]; Contraction of isolated aortic rings from indicated groups in response to the Phenylephrine (10 nM-100 μM) treatment.

: Phenylephrine purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2024 Feb 20:212:477-492. [Abstract]; Rhodamine phalloidin staining was used to analyze the cytoskeleton of cardiomyocytes (red). Neonatal rat cardiomyocytes were pretreated with the different concentration of BRE or DMSO vehicle for 2 h, and then stimulated with 10 μM Phenylephrine hydrochloride (PE) for 48 h.

: Phenylephrine purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2024 Feb 20:212:477-492. [Abstract]; The levels of hypertrophic marker (β-MHC) and fibrosis indice (COL-1) were detected by Western blot analysis in cardiomyocytes. Cells （H9C2 and Neonatal rat cardiomyocytes，NRCMS） were pretreated with the different concentration of BRE or DMSO vehicle for 2 h, and then stimulated with 10 μM Phenylephrine hydrochloride (PE) for 48 h.

: Phenylephrine purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2024 Feb 20:212:477-492. [Abstract]; Intracellular and mitochondrial ROS accumulation were observed and quantitative through DCFH-DA and mitoSOX indicators through confocal laser scanning microscopy (green: DCFH-DA, red: mitoSOX, blue: hoechst 33342). Neonatal rat cardiomyocytes (NRCMs) were pretreated with the different concentration of BRE or DMSO vehicle for 2 h, and then stimulated with 10 μM Phenylephrine hydrochloride (PE) for 48 h.

: Phenylephrine purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2024 Feb 20:212:477-492. [Abstract]; The expression of MFN-1 and MFN-2 were analyzed by Western blot in NRCM cardiomyocytes. Neonatal rat cardiomyocytes (NRCMs) were pretreated with the different concentration of BRE or DMSO vehicle for 2 h, and then stimulated with 10 μM Phenylephrine hydrochloride (PE) for 48 h.

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α adrenergic receptor β adrenergic receptor

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参考文献
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製品説明

(R)-(-)-Phenylephrine is a selective α₁-adrenoceptor agonist primarily used as a decongestant.

IC₅₀ & Target

α adrenergic receptor

Cellular Effect

Cell Line	Type	Value	Description	References
CHO-K1	EC₅₀	0.86 1 Compound: Phenylephrine	Intrinsic activity at human alpha1B adrenergic receptor expressed in CHOK1 cells co-expressing aequorin assessed as calcium mobilization measured for 20 secs in presence of coelenterazine H by luminescence assay Intrinsic activity at human alpha1B adrenergic receptor expressed in CHOK1 cells co-expressing aequorin assessed as calcium mobilization measured for 20 secs in presence of coelenterazine H by luminescence assay	[PMID: 27658792]
CHO-K1	EC₅₀	0.86 1 Compound: Phenylephrine	Intrinsic activity at human alpha1B adrenergic receptor expressed in CHOK1 cells co-expressing aequorin assessed as calcium mobilization measured for 20 secs in presence of coelenterazine H by luminescence assay Intrinsic activity at human alpha1B adrenergic receptor expressed in CHOK1 cells co-expressing aequorin assessed as calcium mobilization measured for 20 secs in presence of coelenterazine H by luminescence assay	[PMID: 27658792]
CHO-K1	EC₅₀	0.86 1 Compound: Phenylephrine	Intrinsic activity at human alpha1B adrenergic receptor expressed in CHOK1 cells co-expressing aequorin assessed as calcium mobilization measured for 20 secs in presence of coelenterazine H by luminescence assay Intrinsic activity at human alpha1B adrenergic receptor expressed in CHOK1 cells co-expressing aequorin assessed as calcium mobilization measured for 20 secs in presence of coelenterazine H by luminescence assay	[PMID: 27658792]

体外実験

(R)-(-)-Phenylephrine is a selective α₁-adrenoceptor agonist with pK_i values of 5.86, 4.87 and 4.70 for α_1D, α_1B and α_1A receptors respectively^[1]^[2].
Phenylephrine promotes cardiac fibroblast proliferation. Phenylephrine activates CaN and evokes NFAT3 nuclear translocation. It suggests that the Ca(²⁺)/CaN/NFAT pathway mediates phenylephrine -induced cardiac fibroblast proliferation, and this pathway might be a possible therapeutic target in cardiac fibrosis^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

体内実験

Perfusion of hearts with 100 μM phenylephrine causes a rapid (maximal at 10 min) 12-fold activation of two p38-MAPK isoforms. α₁-adrenoceptor agonists such as phenylephrine increase the contractility of the heart. Phenylephrine also activates SAPKs/JNKs in neonatal ventricular myocytes^[4].
Phenylephrine could increase the alveolar fluid clearance in high tidal volume-ventilated rats and accelerate the absorption of pulmonary edema^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

167.21

分子式

C₉H₁₃NO₂

CAS 番号

59-42-7

Appearance

Solid

Color

White to light yellow

SMILES

O[C@H](C1=CC(O)=CC=C1)CNC

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶剤 & 溶解度

体外:

DMSO : 50 mg/mL (299.03 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 5 mg/mL (29.90 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.9805 mL	29.9025 mL	59.8050 mL
5 mM	1.1961 mL	5.9805 mL	11.9610 mL
10 mM	0.5981 mL	2.9903 mL	5.9805 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (29.90 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (29.90 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 5 mg/mL (29.90 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 10 mg/mL (59.81 mM); Clear solution; Need ultrasonic and warming

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

純度とドキュメンテーション

純度: 99.98%

Select Batch:

データシート (277 KB) SDS (554 KB)

COA (245 KB) HNMR (200 KB) RP-HPLC (239 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Ford AP, et al. Pharmacological pleiotropism of the human recombinant alpha1A-adrenoceptor: implications foralpha1-adrenoceptor classification. Br J Pharmacol. 1997 Jul;121(6):1127-35. [Content Brief]

[2]. Minneman KP, et al. Selectivity of agonists for cloned alpha 1-adrenergic receptor subtypes. Mol Pharmacol. 1994 Nov;46(5):929-36. [Content Brief]

[3]. Wang J, et al. Phenylephrine promotes cardiac fibroblast proliferation through calcineurin-NFAT pathway. Front Biosci (Landmark Ed). 2016 Jan 1;21:502-13. [Content Brief]

[4]. Lazou A, et al. Activation of mitogen-activated protein kinases (p38-MAPKs, SAPKs/JNKs and ERKs) by the G-protein-coupled receptor agonist phenylephrine in the perfused rat heart. Biochem J. 1998 Jun 1;332 ( Pt 2):459-65. [Content Brief]

[5]. Li NJ, et al. Effect of phenylephrine on alveolar fluid clearance in ventilator-induced lung injury. Chin Med Sci J. 2013 Mar;28(1):1-6. [Content Brief]

動物実験
^[5]

Rats: A total of 170 male Wistar rats are randomLy allocated into 17 groups (n=10) using random number tables. Short-term (40 minutes) mechanical ventilation with high tidal volume is performed to induce lung injury, impair active Na⁺ transport and lung liquid clearance in the rats. Unventilated rats serves as controls. To demonstrate the effect of phenylephrine on alveolar fluid clearance, phenylephrine at different concentrations (10, 1, 0.1, 0.01, and 0.001 μM) is injected into the alveolar space of the HVT ventilated rats^[5].

MedChemExpress (MCE) はこれらの方法の精度を確認していません。こちらは参照専用です。

参考文献

[1]. Ford AP, et al. Pharmacological pleiotropism of the human recombinant alpha1A-adrenoceptor: implications foralpha1-adrenoceptor classification. Br J Pharmacol. 1997 Jul;121(6):1127-35. [Content Brief]

[2]. Minneman KP, et al. Selectivity of agonists for cloned alpha 1-adrenergic receptor subtypes. Mol Pharmacol. 1994 Nov;46(5):929-36. [Content Brief]

[3]. Wang J, et al. Phenylephrine promotes cardiac fibroblast proliferation through calcineurin-NFAT pathway. Front Biosci (Landmark Ed). 2016 Jan 1;21:502-13. [Content Brief]

[4]. Lazou A, et al. Activation of mitogen-activated protein kinases (p38-MAPKs, SAPKs/JNKs and ERKs) by the G-protein-coupled receptor agonist phenylephrine in the perfused rat heart. Biochem J. 1998 Jun 1;332 ( Pt 2):459-65. [Content Brief]

[5]. Li NJ, et al. Effect of phenylephrine on alveolar fluid clearance in ventilator-induced lung injury. Chin Med Sci J. 2013 Mar;28(1):1-6. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	5.9805 mL	29.9025 mL	59.8050 mL	149.5126 mL
	5 mM	1.1961 mL	5.9805 mL	11.9610 mL	29.9025 mL
	10 mM	0.5981 mL	2.9903 mL	5.9805 mL	14.9513 mL
	15 mM	0.3987 mL	1.9935 mL	3.9870 mL	9.9675 mL
	20 mM	0.2990 mL	1.4951 mL	2.9903 mL	7.4756 mL
	25 mM	0.2392 mL	1.1961 mL	2.3922 mL	5.9805 mL
DMSO	30 mM	0.1994 mL	0.9968 mL	1.9935 mL	4.9838 mL
	40 mM	0.1495 mL	0.7476 mL	1.4951 mL	3.7378 mL
	50 mM	0.1196 mL	0.5981 mL	1.1961 mL	2.9903 mL
	60 mM	0.0997 mL	0.4984 mL	0.9968 mL	2.4919 mL
	80 mM	0.0748 mL	0.3738 mL	0.7476 mL	1.8689 mL
	100 mM	0.0598 mL	0.2990 mL	0.5981 mL	1.4951 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Phenylephrine Related Classifications

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量		濃度		体積		分子量 *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）	×	体積 _（開始）	=	濃度 _（終了）	×	体積 _（終了）
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Phenylephrine59-42-7(R)-(-)-Phenylephrine L-PhenylephrineAdrenergic ReceptorBeta ReceptorInhibitorinhibitorinhibit

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フェニレフリン (Synonyms: Phenylephrine; (R)-(-)-Phenylephrine; L-Phenylephrine)

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Complete Stock Solution Preparation Table

Phenylephrine Related Classifications

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フェニレフリン (Synonyms: Phenylephrine; (R)-(-)-Phenylephrine; L-Phenylephrine)

カスタマーレビュー

Other Forms of Phenylephrine:

フェニレフリン 関連抗体

顧客検証

おすすめ

•関連スクリーニングライブラリ:

•関連小分子:

•関連タンパク質:

Adrenergic Receptor アイソフォーム固有の製品をすべて表示:

生物活性

プロトコル

純度とドキュメンテーション

参考文献

カスタマーレビュー

フェニレフリン 関連抗体

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Phenylephrine Related Classifications

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

Keywords:

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