Acetoacetic acid sodium
Based on 5 publication(s) in Google Scholar
Acetoacetic acid sodium is an oxidative stress inducer that affects the antioxidant enzyme system and lipoprotein metabolism. Acetoacetic acid sodium induces oxidative stress by decreasing the mRNA expression and activity of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px), increasing MDA content, and inhibiting very low density lipoprotein (VLDL) assembly by downregulating apolipoprotein ApoB100, ApoE, and low density lipoprotein receptor (LDLR), leading to triglyceride (TG) accumulation in hepatocytes. Acetoacetic acid sodium can be used to study metabolic diseases.
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 623-58-5
- Formula: C4H5NaO3
- Molecular Weight:124.07
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Storage:
-80°C, stored under nitrogen
* The compound is unstable in solutions, freshly prepared is recommended.
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Acetoacetic acid sodium
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Bio/Physico-chemical Assay
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Bio/Physico-chemical Assay
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Biological Activity
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Human Endogenous Metabolite |
In the bovine hepatocyte experiment, Acetoacetic acid sodium (0.6, 2.4, 4.8 mM; 24 h) dose-dependently reduces the mRNA expression and enzyme activity of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px), increases the malondialdehyde (MDA) content, induced oxidative stress, and downregulates the mRNA expression of apolipoprotein B100 (ApoB100), apolipoprotein E (ApoE), and low-density lipoprotein receptor (LDLR), inhibits the assembly of very low-density lipoprotein (VLDL), and leads to the accumulation of intracellular triglycerides (TG) and a decrease in the VLDL content in the culture medium[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Bovine hepatocytes
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Concentration:0 (control), 0.6 mM, 2.4 mM, 4.8 mM
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Incubation Time:24 h
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Result:Significantly downregulated the mRNA expression of antioxidant enzymes (SOD, CAT, GSH-Px) and VLDL-related proteins (ApoB100, ApoE, LDLR) in a dose-dependent manner compared to the control group.
Increased intracellular malondialdehyde (MDA) content, a marker of oxidative stress, while the activities of SOD, CAT, and GSH-Px were reduced.
Resulted intracellular triglyceride (TG) accumulation, and significantly decreased the secretion of VLDL into the culture medium in a dose-dependent manner.
Chemical Information
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CAS No. 623-58-5
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Appearance Solid
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Molecular Weight 124.07
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Formula C4H5NaO3
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Color White to off-white
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SMILES
CC(CC(O[Na])=O)=O
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Structure Classification
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Initial Source
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Shipping
Shipping with dry ice.
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Storage
-80°C, stored under nitrogen
* The compound is unstable in solutions, freshly prepared is recommended.
Publications (5)
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Journal Impact Factor
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Most Recent
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Nat Metab
β-hydroxybutyrate inhibits Plasmodium falciparum development and confers protection against malaria in mice. [Abstract]2025 Jul;7(7):1330-1343. PMID: 40410577 -
J Nanobiotechnology
The ketone body β-hydroxybutyrate alleviates CoCrMo alloy particles induced osteolysis by regulating NLRP3 inflammasome and osteoclast differentiation. [Abstract]2022 Mar 9;20(1):120. PMID: 35264201 -
Am J Physiol Cell Physiol
Acetoacetate and D- and L-β-hydroxybutyrate have distinct effects on basal and insulin-stimulated glucose uptake in L6 skeletal muscle cells. [Abstract]2024 Jun 1;326(6):C1710-C1720. PMID: 38708524
Acetoacetic acid sodium purchased from MedChemExpress. Usage Cited in: Am J Physiol Cell Physiol. 2024 Jun 1;326(6):C1710-C1720. [Abstract]
Acetoacetic acid sodium (1 mM; 3 h) showed no effect on theexpression of ACAT or SCOT in L6 rat myotubes.
Acetoacetic acid sodium purchased from MedChemExpress. Usage Cited in: Am J Physiol Cell Physiol. 2024 Jun 1;326(6):C1710-C1720. [Abstract]
Acetoacetic acid sodium (0.2, 1 mM; 3 h) showed significantly increased thebasal translocation of GLUT4 to the membrane relative toglucose in 0.2 mM, but no differences were found for the other condi-tions in L6 GLUT4 mycmyotubes.
Acetoacetic acid sodium purchased from MedChemExpress. Usage Cited in: Am J Physiol Cell Physiol. 2024 Jun 1;326(6):C1710-C1720. [Abstract]
Acetoacetic acid sodium (0.2, 1 mM; 3 h; at the end ofthe 3 h treatment, 10 or 100 nM insulin for 20 min) decreased thebasal translocation of GLUT4 to the membrane in 0.2 mM with 100 nM insulin in L6 GLUT4 mycmyotubes.
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J Leukoc Biol
2023 Jun 1;113(6):577-587. PMID: 36999365 -
Solvent & Solubility
H2O : 100 mg/mL (806.00 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.04 mg/mL (8.38 mM); Clear solution
This protocol yields a clear solution of ≥ 1.04 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.4 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (806.00 mM); Clear solution; Need ultrasonic
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (393 KB)
- English - EN (393 KB)
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- Deutsch - DE (393 KB)
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- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| H2O | 1 mM | 8.0600 mL | 40.2998 mL | 80.5997 mL | 201.4992 mL |
| 5 mM | 1.6120 mL | 8.0600 mL | 16.1199 mL | 40.2998 mL | |
| 10 mM | 0.8060 mL | 4.0300 mL | 8.0600 mL | 20.1499 mL | |
| 15 mM | 0.5373 mL | 2.6867 mL | 5.3733 mL | 13.4333 mL | |
| 20 mM | 0.4030 mL | 2.0150 mL | 4.0300 mL | 10.0750 mL | |
| 25 mM | 0.3224 mL | 1.6120 mL | 3.2240 mL | 8.0600 mL | |
| 30 mM | 0.2687 mL | 1.3433 mL | 2.6867 mL | 6.7166 mL | |
| 40 mM | 0.2015 mL | 1.0075 mL | 2.0150 mL | 5.0375 mL | |
| 50 mM | 0.1612 mL | 0.8060 mL | 1.6120 mL | 4.0300 mL | |
| 60 mM | 0.1343 mL | 0.6717 mL | 1.3433 mL | 3.3583 mL | |
| 80 mM | 0.1007 mL | 0.5037 mL | 1.0075 mL | 2.5187 mL | |
| 100 mM | 0.0806 mL | 0.4030 mL | 0.8060 mL | 2.0150 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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