Acetoacetic acid sodium

Name: Acetoacetic acid sodium
Brand: MedChemExpress
SKU: HY-112540B
Rating: 5.0 (3 reviews)

Cat. No.: HY-112540B Purity: ≥98.0%: FAQs
Data Sheet
SDS

COA
Handling Instructions Technical Support

Acetoacetic acid sodium is an oxidative stress inducer that affects the antioxidant enzyme system and lipoprotein metabolism. Acetoacetic acid sodium induces oxidative stress by decreasing the mRNA expression and activity of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px), increasing MDA content, and inhibiting very low density lipoprotein (VLDL) assembly by downregulating apolipoprotein ApoB100, ApoE, and low density lipoprotein receptor (LDLR), leading to triglyceride (TG) accumulation in hepatocytes. Acetoacetic acid sodium can be used to study metabolic diseases.

For research use only. We do not sell to patients.

Acetoacetic acid sodium Chemical Structure

CAS No. : 623-58-5

Size	Stock	Quantity
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
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500 mg	Get quote

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Customer Review

Based on 3 publication(s) in Google Scholar

Other Forms of Acetoacetic acid sodium:

Acetoacetic acid Get quote
Acetoacetic acid lithium Get quote

3 Publications Citing Use of MCE Acetoacetic acid sodium

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Human Endogenous Metabolite

In Vitro

In the bovine hepatocyte experiment, Acetoacetic acid sodium (0.6, 2.4, 4.8 mM; 24 h) dose-dependently reduces the mRNA expression and enzyme activity of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px), increases the malondialdehyde (MDA) content, induced oxidative stress, and downregulates the mRNA expression of apolipoprotein B100 (ApoB100), apolipoprotein E (ApoE), and low-density lipoprotein receptor (LDLR), inhibits the assembly of very low-density lipoprotein (VLDL), and leads to the accumulation of intracellular triglycerides (TG) and a decrease in the VLDL content in the culture medium^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	Bovine hepatocytes
Concentration:	0 (control), 0.6 mM, 2.4 mM, 4.8 mM
Incubation Time:	24 h
Result:	Significantly downregulated the mRNA expression of antioxidant enzymes (SOD, CAT, GSH-Px) and VLDL-related proteins (ApoB100, ApoE, LDLR) in a dose-dependent manner compared to the control group. Increased intracellular malondialdehyde (MDA) content, a marker of oxidative stress, while the activities of SOD, CAT, and GSH-Px were reduced. Resulted intracellular triglyceride (TG) accumulation, and significantly decreased the secretion of VLDL into the culture medium in a dose-dependent manner.

Molecular Weight

124.07

Formula

C₄H₅NaO₃

CAS No.

623-58-5

Appearance

Solid

Color

White to off-white

SMILES

CC(CC(O[Na])=O)=O

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Shipping

Shipping with dry ice.

Storage

-80°C, stored under nitrogen

*The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (806.00 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	8.0600 mL	40.2998 mL	80.5997 mL
5 mM	1.6120 mL	8.0600 mL	16.1199 mL
10 mM	0.8060 mL	4.0300 mL	8.0600 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.04 mg/mL (8.38 mM); Clear solution

This protocol yields a clear solution of ≥ 1.04 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.4 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 1.04 mg/mL (8.38 mM); Clear solution

This protocol yields a clear solution of ≥ 1.04 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.4 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (806.00 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (273 KB) SDS (393 KB)

COA (246 KB) HNMR (134 KB)

Handling Instructions (2659 KB)

References

[1]. Shi X, et al. Acetoacetic acid induces oxidative stress to inhibit the assembly of very low density lipoprotein in bovine hepatocytes. J Dairy Res. 2016 Nov;83(4):442-446. [Content Brief]

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	8.0600 mL	40.2998 mL	80.5997 mL	201.4992 mL
	5 mM	1.6120 mL	8.0600 mL	16.1199 mL	40.2998 mL
	10 mM	0.8060 mL	4.0300 mL	8.0600 mL	20.1499 mL
	15 mM	0.5373 mL	2.6867 mL	5.3733 mL	13.4333 mL
	20 mM	0.4030 mL	2.0150 mL	4.0300 mL	10.0750 mL
	25 mM	0.3224 mL	1.6120 mL	3.2240 mL	8.0600 mL
	30 mM	0.2687 mL	1.3433 mL	2.6867 mL	6.7166 mL
	40 mM	0.2015 mL	1.0075 mL	2.0150 mL	5.0375 mL
	50 mM	0.1612 mL	0.8060 mL	1.6120 mL	4.0300 mL
	60 mM	0.1343 mL	0.6717 mL	1.3433 mL	3.3583 mL
	80 mM	0.1007 mL	0.5037 mL	1.0075 mL	2.5187 mL
	100 mM	0.0806 mL	0.4030 mL	0.8060 mL	2.0150 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.