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  4. Acridine Orange zinc chloride salt

Acridine Orange zinc chloride salt  (Synonyms: Euchrysine 3RX)

製品番号: HY-D0942
COA 取扱説明書 Technical Support

Acridine Orange (Euchrysine 3RX) zinc chloride salt is a cell-penetrable nucleic acid-selective fluorescent dye. Acridine Orange zinc chloride salt produces orange fluorescence when it binds to ssDNA or RNA, and green fluorescence when it binds to dsDNA (Ex: 488 nM; Em: green fluorescence at 530 nm, orange fluorescence at 640 nm).

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CAS 番号 : 10127-02-3

容量 価格（税別） 在庫状況 数量
>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 66 在庫あり
Solution
10 mM * 1 mL in DMSO USD 66 在庫あり
Solid
100 mg $60 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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カスタマーレビュー

Based on 38 publication(s) in Google Scholar

Other Forms of Acridine Orange zinc chloride salt:

Acridine Orange zinc chloride salt 関連抗体

Top Publications Citing Use of Products

顧客検証

Cell Imaging/Staining
IF

    Acridine Orange zinc chloride salt purchased from MedChemExpress. Usage Cited in: Cell Mol Immunol. 2025 Mar;22(3):282-299.  [Abstract]

    Human PBMCs (H-PBMCs) and Jurkat cells were treated with OA (5, 50, or 500 μM) or 200 ng/mL PMA for 24 hours, after which the cells were stained with acridine orange (AO).

    Acridine Orange zinc chloride salt purchased from MedChemExpress. Usage Cited in: Nat Commun. 2023 Jun 30;14(1):3877.  [Abstract]

    Acridine Orange (AO, 5 μM) was added immediately after the ultrasound treatment and was incubated for 30 min at 37°C before confocal microscopy.

    Acridine Orange zinc chloride salt purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2021 Oct;11(10):3178-3192.

    The Jurkat cells were treated with LW-218 (8 μmol/L) for 0, 1, 2, and 4 h. Before collecting, the cells were stained with 5 μmol/L Acridine Orange hydrochloride solution for 15 min.

    Acridine Orange zinc chloride salt purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2021 Jan 13;12(1):80.  [Abstract]

    The Jurkat cells treated with 25 μM FV-429 for 12 h were stained with Acridine Orange hydrochloride (AO, 5 μM; 15 min) before collection, and the AO fluorescence was determined by a laser confocal microscope. Scale bar: 10 μm.

    Acridine Orange zinc chloride salt purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2021 Jan 13;12(1):80.  [Abstract]

    The Jurkat and Hut102 cells treated with 25 μM FV-429 for 12 h were stained with Acridine Orange hydrochloride (AO, 5 μM; 15 min) staining before collecting, and the AO fluorescences were determined by laser confocal microscope. Scale bar: 5 μm.

    Acridine Orange zinc chloride salt purchased from MedChemExpress. Usage Cited in: Cell Prolif. 2022 Jan;55(1):e13172.  [Abstract]

    CLSM images of acridine orange (AO)-stained osteoclasts cultured on PDMS substrates were taken; the cells were stained with 1 μM AO at 37°C for 20 min.

    Acridine Orange zinc chloride salt purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2020 Jul 30;11(7):607.  [Abstract]

    Depending on the acidity, Acridine Orange hydrochloride (AO) causes autophagic lysosomes to appear as orange/red fluorescent vesicles, while it caused nuclei to appear green. AO staining demonstrates that LA dose-dependently increased the numbers of intracellular acidic compartments.

    Parasite アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Amebae Coccidia Leishmania Mite Plasmodium Toxoplasma Trypanosoma Schistosome

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    • 純度とドキュメンテーション

    • 参考文献

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    製品説明

    Acridine Orange (Euchrysine 3RX) zinc chloride salt is a cell-penetrable nucleic acid-selective fluorescent dye. Acridine Orange zinc chloride salt produces orange fluorescence when it binds to ssDNA or RNA, and green fluorescence when it binds to dsDNA (Ex: 488 nM; Em: green fluorescence at 530 nm, orange fluorescence at 640 nm)[1][2][3].

    体外実験

    Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
    1. Cell Counting with AO/PI (Live/Dead Assay)
    (1) Prepare Sample: Mix equal volumes of cell suspension and 1-5 μg/mL Acridine Orange in PBS.
    (2) Incubate: No incubation is typically required for simple counting; however, 5-15 minutes in the dark can improve results.
    (3) Analyze: Use a fluorescence microscope with blue excitation (e.g., 488 nm).
    (4) Result Interpretation: Live nucleated cells display green fluorescence, while dead cells with compromised membranes display red fluorescence.
    2. Microbial Staining (Slide Protocol)
    (1) Prepare Sample: Prepare a smear on a clean glass slide and allow to air dry.
    (2) Fixation: Fix the smear using methanol (2-3 minutes).
    (3) Staining: Flood the slide with 0.1% Acridine Orange stain and allow it to sit for 2 minutes.
    (4) Rinse & Decolorize: Rinse with tap water, then wash with alcoholic saline (10% ethanol in 0.9% NaCl) for 1-2 minutes to reduce background.
    (5) Imaging: Air dry and examine using a fluorescence microscope.
    3. Cell Cycle Analysis (Flow Cytometry)
    (1) Suspension: Aliquot 105-106 cells in 100 μl PBS.
    (2) Permeabilization: Add 0.5 mL of Buffer I (0.1% Triton X-100, 0.2M Sucrose, 0.1M Citric Acid) at room temperature.
    (3) Staining: Add 0.5 mL of Staining Buffer II (0.1 M NaCl, 0.1 M Sodium Phosphate/Citrate buffer, pH 3.8, with 20 μg/mL AO).
    (4) Analyze: Immediately run on a flow cytometer.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Acridine Orange zinc chloride salt 関連抗体
    体内実験

    Acridine Orange (0.1 mg/kg, i.v., dogs) zinc chloride salt shows no clinical signs of toxicity and no abnormalities were seen in the blood within 30 days[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    分子量

    438.10

    分子式

    C17H20Cl3N3Zn
    CAS 番号
    Appearance

    Solid

    Color

    Brown to reddish brown

    SMILES

    Cl[Zn]Cl.CN(C)C1=CC=C2C(N=C3C=C(N(C)C)C=CC3=C2)=C1.Cl
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    4°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
    溶剤 & 溶解度
    体外: 

    DMSO : 100 mg/mL (228.26 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 10 mg/mL (22.83 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.2826 mL 11.4129 mL 22.8258 mL
    5 mM 0.4565 mL 2.2826 mL 4.5652 mL
    10 mM 0.2283 mL 1.1413 mL 2.2826 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

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    濃度 （終了）

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    純度とドキュメンテーション
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 2.2826 mL 11.4129 mL 22.8258 mL 57.0646 mL
    5 mM 0.4565 mL 2.2826 mL 4.5652 mL 11.4129 mL
    10 mM 0.2283 mL 1.1413 mL 2.2826 mL 5.7065 mL
    15 mM 0.1522 mL 0.7609 mL 1.5217 mL 3.8043 mL
    20 mM 0.1141 mL 0.5706 mL 1.1413 mL 2.8532 mL
    DMSO 25 mM 0.0913 mL 0.4565 mL 0.9130 mL 2.2826 mL
    30 mM 0.0761 mL 0.3804 mL 0.7609 mL 1.9022 mL
    40 mM 0.0571 mL 0.2853 mL 0.5706 mL 1.4266 mL
    50 mM 0.0457 mL 0.2283 mL 0.4565 mL 1.1413 mL
    60 mM 0.0380 mL 0.1902 mL 0.3804 mL 0.9511 mL
    80 mM 0.0285 mL 0.1427 mL 0.2853 mL 0.7133 mL
    100 mM 0.0228 mL 0.1141 mL 0.2283 mL 0.5706 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Acridine Orange zinc chloride salt Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Acridine Orange zinc10127-02-3Euchrysine 3RXParasiteFluorescent DyeDNA StainNucleic aciddsDNARNAssDNAInhibitorinhibitorinhibit

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    製品名:
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    製品番号:
    HY-D0942
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