Anti-CD24 Antibody (SWA11)

製品番号: HY-P991196 純度: 99.18%: Data Sheet SDS COA
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Anti-CD24 Antibody (SWA11) is a human IgG1 monoclonal antibody targeting CD24, with high affinity and specificity for CD24-expressing tumor cells. Anti-CD24 Antibody (SWA11) triggers endocytosis, alters STAT3-dependent gene expression, regulates Src phosphorylation, and blocks CD24-mediated signaling pathways. Anti-CD24 Antibody (SWA11) is applicable to cancer-related research such as lung cancer and ovarian cancer. Recommended isotype control: Human IgG1 kappa, Isotype Control (HY-P99001).

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容量	価格（税別）	在庫状況	数量
1 mg	$350	在庫あり	Estimated Time of Arrival: December 31
5 mg	$1050	在庫あり	Estimated Time of Arrival: December 31
10 mg	$1680	在庫あり	Estimated Time of Arrival: December 31
25 mg	$3000	在庫あり	Estimated Time of Arrival: December 31
50 mg		お問い合わせ
100 mg		お問い合わせ

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製品説明

Isotype

Human IgG1 kappa

Recommend Isotype Controls

Human IgG1 kappa, Isotype Control

Species Reactivity

Human

IC₅₀ & Target

CD24

体外実験

Anti-CD24 Antibody (SWA11) inhibits the proliferation of human pancreatic cancer cells, ovarian cancer cells and lung cancer cells; it enhances the anti-tumor efficacy of Gemcitabine (HY-17026), 5-Fluorouridine (HY-107856), Oxaliplatin (HY-17371), Doxorubicin (HY-15142A), Paclitaxel (HY-B0015) and Irinotecan (HY-16562), and also enhances the anti-tumor efficacy of Cisplatin (HY-17394) in human embryonic carcinoma cells^[2].
Anti-CD24 Antibody (SWA11) binds to CD24 on the surface of CD24-positive tumor cells, thereby triggering endocytosis, regulating STAT3-dependent gene expression and Src phosphorylation, directly inhibiting the proliferation, adhesion and invasion abilities of tumor cells. Meanwhile, it activates antibody-dependent cell-mediated cytotoxicity (ADCC) and the complement system to enhance anti-tumor efficacy^[2].
Anti-CD24 Antibody (SWA11) conjugated with doxorubicin, namely SWA11-DOX, can be rapidly internalized by small cell lung cancer cells^[2].
Anti-CD24 Antibody (SWA11) (1 μg/mL) specifically detects CD24 protein in human colorectal cancer HT-29 cell lysates^[3].
Anti-CD24 Antibody (SWA11) (1.6-16 μg/mL; 1 h) selectively binds to CD24-positive human colorectal cancer cell line HT-29, while showing no significant binding to CD24-negative human colorectal cancer cell line HCT116^[3].
Anti-CD24 Antibody (SWA11) (10 μg/mL; 30 min) shows specific binding to CD24-positive human colorectal cancer cells, which can be detected by flow cytometry^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

ELISA Assay^[3]

Cell Line:	HT-29 (CD24-positive), HCT116 (CD24-negative) human CRC cell lines
Concentration:	1.6, 3.2, 8, 16 μg/mL
Incubation Time:	1 h
Result:	Measured high absorbance in HT-29 cells across all tested concentrations. Showed negligible absorbance in HCT116 cells across all tested concentrations.

体内実験

Anti-CD24 Antibody (SWA11) (10 mg/kg; 4 times with 5-day interval) reduces A549 lung carcinoma xenograft growth relative to controls, decreases tumor cell proliferation and blood vessel density, and significantly alters the intratumoral cytokine milieu in female SCID beige mice^[1].
Anti-CD24 Antibody (SWA11) (10 mg/kg; twice weekly; 4 weeks) reduces SKOV3ip ovarian carcinoma xenograft endpoint weight by relative to controls, decreases tumor cell proliferation and blood vessel density, and increases macrophage infiltration in female athymic CD1 nu/nu mice^[1].
Anti-CD24 Antibody (SWA11) (10 mg/kg; once per treatment cycle, 3 total cycles, 1 day prior to Gemcitabine (HY-17026)) potentiates the anti-tumor efficacy of gemcitabine, reducing A549 lung carcinoma xenograft growth in female SCID beige mice^[1].
Anti-CD24 Antibody (SWA11) slows tumor growth in human colorectal cancer xenografts, modulates the tumor immune microenvironment, and enhances the anti-tumor efficacy of several chemotherapy agents^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	SCID beige (female, 4- to 5-week-old; subcutaneous xenograft of A549 human lung adenocarcinoma cells)^[1]
Dosage:	10 mg/kg
Administration:	4 times with 5-day interval
Result:	Reduced relative tumor volume increase by day 21 after first injection. Reduced percentage of Ki-67-positive proliferating tumor cells. Increased intratumoral levels of CCL1, CCL2/MCP-1, CCL12, CXCL9/MIG, CXCL10, CXCL11, IFN-γ, IL-1α, IL-1β, and IL-1Ra.

Animal Model:	athymic CD1 nu/nu (female; intraperitoneal inoculation of luciferase-transfected SKOV3ip human ovarian carcinoma cells)^[1]
Dosage:	10 mg/kg
Administration:	8 times; twice weekly for 4 weeks
Result:	Reduced endpoint tumor weight. Reduced percentage of Ki-67-positive proliferating tumor cells. Increased intratumoral levels of CCL13, sICAM-1, IFN-γ, IL-1α, IL-1Ra, IL-12 p70, IL-16, IL-17, CXCL9/MIG, and CCL5/RANTES. Increased infiltration of SKOV3ip tumors by F4/80-positive macrophages.

Gene ID

100133941 [NCBI]

Accession

P25063-1

Application

ELISA, FACS, Functional assay

Conjugated

Unconjugated

Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.

分子量

146.67 kDa

Appearance

Liquid

Color

Colorless to light yellow

SMILES

[Anti-CD24 Antibody (SWA11)]

輸送条件

Shipping with dry ice.

Formulation

Please refer to the lot-specific COA for specific buffer information.

保管条件

Please store the product under the recommended conditions in the Certificate of Analysis.

Biological Activity

Flow cytometric analysis of 1×10⁶ MCF-7 cells with Anti-CD24 Antibody (SWA11) (HY-P991196, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. Alexa Goat Anti-Human IgG H&L (AF488) (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG1 kappa Isotype Control (HY-P99001, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

純度とドキュメンテーション

純度: 99.18%

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データシート (264 KB) SDS (251 KB)

COA (232 KB) SDS-PAGE (63 KB) SEC-HPLC (344 KB)

Inhibitory Antibodies User Guide (603 KB)

参考文献

[1]. Salnikov AV, et al. Antibody targeting of CD24 efficiently retards growth and influences cytokine milieu in experimental carcinomas. Br J Cancer. 2013;108(7):1449-1459. [Content Brief]

[2]. Huang S, et al. Checkpoint CD24 function on tumor and immunotherapy. Front Immunol. 2024;15:1367959. [Content Brief]

[3]. Shapira S, et al. An immunoconjugate of anti-CD24 and Pseudomonas exotoxin selectively kills human colorectal tumors in mice. Gastroenterology. 2011;140(3):935-946. [Content Brief]