2. Cell Cycle/DNA Damage
    Apoptosis
  3. DNA/RNA Synthesis
    Apoptosis
  4. 5-Fluorouridine

5-Fluorouridine 

Cat. No.: HY-107856 Purity: 98.01%
COA Handling Instructions

5-Fluorouridine, a metabolite of 5-fluorouracil (HY-90006), is a potent ribozyme self-cleavage inhibitor. 5-Fluorouridine incorporates into both total and poly A RNA and has antiproliferative activity. 5-Fluorouridine induces apoptosis.

For research use only. We do not sell to patients.

5-Fluorouridine Chemical Structure

5-Fluorouridine Chemical Structure

CAS No. : 316-46-1

Size Price Stock Quantity
Free Sample (0.1 - 0.5 mg)   Apply Now  
Solution
10 mM * 1 mL in DMSO USD 55 In-stock
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
 USD 55 In-stock
Solid
100 mg USD 50 In-stock
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

5-Fluorouridine, a metabolite of 5-fluorouracil (HY-90006), is a potent ribozyme self-cleavage inhibitor. 5-Fluorouridine incorporates into both total and poly A RNA and has antiproliferative activity. 5-Fluorouridine induces apoptosis[1][2][3].

In Vitro

5-Fluorouridine (167 μM; 24-96 h) inhibits MKN45 cells and MKN28 cells in a time-dependent manner[1].
5-Fluorouridine (10 μM; 24 h; HCT-116 cells) induces apoptosis and increases the percentage of apoptotic cells. 5-Fluorouridine upregulates the expression of 33 genes, including a group of genes as growth factors, cytokines and chemokines (e.g. IL-3, IL -4, B-cell growth factor 1 and stem cell growth factor)[2].
5-Fluorouridine (10 μM; 8-24 h; HCT-116 cells) incorporates into both total and poly A RNA[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo

5-Fluorouridine (567-1500 mg/kg; i.p.; daily, for 20 d) induce gastrointestinal toxicity in the mice[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male CBA/J mice (17-20 g; 6-8 weeks of age)[3]
Dosage: Intraperitoneal injection; daily, 20 days
Administration: 567, 700, 900, 1100, 1300, and 1500 mg/kg
Result: 567, 700, 900, 1100, 1300, and 1500 mg/kg
Clinical Trial
Molecular Weight

262.19

Appearance

Solid

Formula

C9H11FN2O6
CAS No.
SMILES
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (381.40 mM)

H2O : 100 mg/mL (381.40 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.8140 mL 19.0701 mL 38.1403 mL
5 mM 0.7628 mL 3.8140 mL 7.6281 mL
10 mM 0.3814 mL 1.9070 mL 3.8140 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  PBS

    Solubility: 110 mg/mL (419.54 mM); Clear solution; Need ultrasonic

  • 2.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (7.93 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (7.93 mM); Clear solution

  • 4.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (7.93 mM); Clear solution

*All of the co-solvents are available by MCE.
Purity & Documentation

Purity: 98.01%

COA (253 KB) HNMR (268 KB) RP-HPLC (243 KB) MS (185 KB)

Handling Instructions (2659 KB)
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Keywords:

5-Fluorouridine316-46-1DNA/RNA SynthesisApoptosisRNAribozyme self-cleavageantiproliferativeHCT-116 cellsInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Salutation

Applicant Name *

 

Email address *

Phone number *

 

Organization name *

Department *

 

Requested quantity *

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
5-Fluorouridine
Cat. No.:
HY-107856
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email address *

Phone number

 

Department *

Organization name *

 

Country or Region *

Remarks

Request for HNMR Report

We have received your request and will respond to you as soon as possible.