2. Cell Cycle/DNA Damage Apoptosis
  3. DNA/RNA Synthesis Apoptosis
  4. 5-Fluorouridine

5-Fluorouridine, a metabolite of 5-fluorouracil (HY-90006), is a potent ribozyme self-cleavage inhibitor. 5-Fluorouridine incorporates into both total and poly A RNA and has antiproliferative activity. 5-Fluorouridine induces apoptosis.

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5-Fluorouridine Chemical Structure

5-Fluorouridine Chemical Structure

CAS No. : 316-46-1

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Based on 1 publication(s) in Google Scholar

5-Fluorouridine Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE 5-Fluorouridine

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

5-Fluorouridine, a metabolite of 5-fluorouracil (HY-90006), is a potent ribozyme self-cleavage inhibitor. 5-Fluorouridine incorporates into both total and poly A RNA and has antiproliferative activity. 5-Fluorouridine induces apoptosis[1][2][3].

In Vitro

5-Fluorouridine (167 μM; 24-96 h) inhibits MKN45 cells and MKN28 cells in a time-dependent manner[1].
? 5-Fluorouridine (10 μM; 24 h; HCT-116 cells) induces apoptosis and increases the percentage of apoptotic cells. 5-Fluorouridine upregulates the expression of 33 genes, including a group of genes as growth factors, cytokines and chemokines (e.g. IL-3, IL -4, B-cell growth factor 1 and stem cell growth factor)[2].
? 5-Fluorouridine (10 μM; 8-24 h; HCT-116 cells) incorporates into both total and poly A RNA[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

5-Fluorouridine Related Antibodies
In Vivo

5-Fluorouridine (567-1500 mg/kg; i.p.; daily, for 20 d) induce gastrointestinal toxicity in the mice[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male CBA/J mice (17-20 g; 6-8 weeks of age)[3]
Dosage: Intraperitoneal injection; daily, 20 days
Administration: 567, 700, 900, 1100, 1300, and 1500 mg/kg
Result: 567, 700, 900, 1100, 1300, and 1500 mg/kg
Molecular Weight

262.19

Formula

C9H11FN2O6
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N2C(NC(C(F)=C2)=O)=O)O1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (381.40 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 100 mg/mL (381.40 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.8140 mL 19.0701 mL 38.1403 mL
5 mM 0.7628 mL 3.8140 mL 7.6281 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (7.93 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (7.93 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 110 mg/mL (419.54 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 98.01%

COA (253 KB) HNMR (268 KB) RP-HPLC (243 KB) MS (185 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 3.8140 mL 19.0701 mL 38.1403 mL 95.3507 mL
5 mM 0.7628 mL 3.8140 mL 7.6281 mL 19.0701 mL
10 mM 0.3814 mL 1.9070 mL 3.8140 mL 9.5351 mL
15 mM 0.2543 mL 1.2713 mL 2.5427 mL 6.3567 mL
20 mM 0.1907 mL 0.9535 mL 1.9070 mL 4.7675 mL
25 mM 0.1526 mL 0.7628 mL 1.5256 mL 3.8140 mL
30 mM 0.1271 mL 0.6357 mL 1.2713 mL 3.1784 mL
40 mM 0.0954 mL 0.4768 mL 0.9535 mL 2.3838 mL
50 mM 0.0763 mL 0.3814 mL 0.7628 mL 1.9070 mL
60 mM 0.0636 mL 0.3178 mL 0.6357 mL 1.5892 mL
80 mM 0.0477 mL 0.2384 mL 0.4768 mL 1.1919 mL
100 mM 0.0381 mL 0.1907 mL 0.3814 mL 0.9535 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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5-Fluorouridine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

5-Fluorouridine316-46-1DNA/RNA SynthesisApoptosisRNAribozyme self-cleavageantiproliferativeHCT-116 cellsInhibitorinhibitorinhibit

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