2. Metabolic Enzyme/Protease Cell Cycle/DNA Damage Apoptosis
  3. Drug Metabolite DNA/RNA Synthesis Apoptosis
  4. 5-Fluorouridine

5-Fluorouridine, a metabolite of5-Fluorouracil (HY-90006), is a potent ribozyme self-cleavage inhibitor. 5-Fluorouridine incorporates into both total and poly A RNA and has antiproliferative activity. 5-Fluorouridine induces apoptosis.

For research use only. We do not sell to patients.

CAS No. : 316-46-1

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Customer Review

Based on 9 publication(s) in Google Scholar

Other Forms of 5-Fluorouridine:

5-Fluorouridine Related Antibodies

Top Publications Citing Use of Products

    5-Fluorouridine purchased from MedChemExpress. Usage Cited in: Cell Rep. 2025 Jan 13;44(1):115179.  [Abstract]

    Cell survival at 72 h after treatment with 5-azacytidine (5-AzaCd) or 5-fluorouridine (5-FUd, 0-100 μM) with or without dTAGv1 (100 nM) in HCT116 UCK2-FKBP12F36V K/I cells. dTAGv1-mediated degradation of UCK2 results in 156-fold resistance to 5-FUd and 1.6-fold to 5-AzaCd. Mean ± SD, n = 12 biological replicates.

    View All DNA/RNA Synthesis Isoform Specific Products:

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    RNASE L DNA Polymerases RNA Polymerases Helicases DNA Ligase

    • Biological Activity

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    Description

    5-Fluorouridine, a metabolite of5-Fluorouracil (HY-90006), is a potent ribozyme self-cleavage inhibitor. 5-Fluorouridine incorporates into both total and poly A RNA and has antiproliferative activity. 5-Fluorouridine induces apoptosis[1][2][3].

    Cellular Effect
    Cell Line Type Value Description References
    A549 IC50
    1.9 μM
    Compound: FUrd (5-fluorouridine)
    Compound was tested for its inhibitory effect on the growth of A-549 tumor cell line from lung.
    Compound was tested for its inhibitory effect on the growth of A-549 tumor cell line from lung.
    		10.1016/0960-894X(96)00339-3
    CCRF-CEM IC50
    5.0 x 10-8 M
    Compound: 2
    Concentration required for the 50% inhibition of growth of CCRF-CEM human leukemia cells in culture
    Concentration required for the 50% inhibition of growth of CCRF-CEM human leukemia cells in culture
    		[PMID: 7120290]
    COLO 320DM IC50
    0.0054 μM
    Compound: FUrd (5-fluorouridine)
    Compound was tested for its inhibitory effect on the growth of COLO 320DM tumor cell line from colon.
    Compound was tested for its inhibitory effect on the growth of COLO 320DM tumor cell line from colon.
    		10.1016/0960-894X(96)00339-3
    DLD-1 IC50
    0.073 μM
    Compound: FUrd (5-fluorouridine)
    Compound was tested for its inhibitory effect on the growth of DLD-1 tumor cell line from colon.
    Compound was tested for its inhibitory effect on the growth of DLD-1 tumor cell line from colon.
    		10.1016/0960-894X(96)00339-3
    FM3A IC50
    0.014 μM
    Compound: 5-FUrd
    Cytostatic activity against mouse FM3A cells after 2 days by coulter counting analysis
    Cytostatic activity against mouse FM3A cells after 2 days by coulter counting analysis
    		[PMID: 21330014]
    HCT-15 IC50
    0.047 μM
    Compound: FUrd (5-fluorouridine)
    Compound was tested for its inhibitory effect on the growth of HCT-15 tumor cell line from colon.
    Compound was tested for its inhibitory effect on the growth of HCT-15 tumor cell line from colon.
    		10.1016/0960-894X(96)00339-3
    HT-1080 IC50
    0.041 μM
    Compound: FUrd (5-fluorouridine)
    Compound was tested for its inhibitory effect on the growth of HT1080 sarcoma tumor cell line.
    Compound was tested for its inhibitory effect on the growth of HT1080 sarcoma tumor cell line.
    		10.1016/0960-894X(96)00339-3
    HeLa IC50
    0.016 μM
    Compound: 5-FUrd
    Cytostatic activity against human HeLa cells in presence of 500 uM uracil
    Cytostatic activity against human HeLa cells in presence of 500 uM uracil
    		[PMID: 21330014]
    HeLa IC50
    0.017 μM
    Compound: 5-FUrd
    Cytostatic activity against human HeLa cells in presence of 20 uM thymidine
    Cytostatic activity against human HeLa cells in presence of 20 uM thymidine
    		[PMID: 21330014]
    HeLa IC50
    0.022 μM
    Compound: 5-FUrd
    Cytostatic activity against human HeLa cells in presence of 20 uM 2'-deoxyuridine
    Cytostatic activity against human HeLa cells in presence of 20 uM 2'-deoxyuridine
    		[PMID: 21330014]
    HeLa IC50
    1.6 μM
    Compound: 5-FUrd
    Cytostatic activity against human HeLa cells in presence of 500 uM uridine
    Cytostatic activity against human HeLa cells in presence of 500 uM uridine
    		[PMID: 21330014]
    IM-9 IC50
    < 0.01 μg/mL
    Compound: 5-F-Urd
    In vitro antitumor activity in IM-9 cell lines
    In vitro antitumor activity in IM-9 cell lines
    		[PMID: 8648611]
    K562 IC50
    0.026 μg/mL
    Compound: 5-F-Urd
    In vitro antitumor activity in K-562 cell lines
    In vitro antitumor activity in K-562 cell lines
    		[PMID: 8648611]
    KKLS IC50
    0.016 μM
    Compound: FUrd (5-fluorouridine)
    Compound was tested for its inhibitory effect on the growth of KKLS tumor cell line from stomach.
    Compound was tested for its inhibitory effect on the growth of KKLS tumor cell line from stomach.
    		10.1016/0960-894X(96)00339-3
    L1210 IC50
    0.0075 μM
    Compound: 5-FUrd
    Cytostatic activity against mouse L1210 cells in presence of 500 uM uracil
    Cytostatic activity against mouse L1210 cells in presence of 500 uM uracil
    		[PMID: 21330014]
    L1210 IC50
    0.014 μM
    Compound: 5-FUrd
    Cytostatic activity against mouse L1210 cells after 2 days by coulter counting analysis
    Cytostatic activity against mouse L1210 cells after 2 days by coulter counting analysis
    		[PMID: 21330014]
    L1210 IC50
    0.026 μM
    Compound: 5-FUrd
    Cytostatic activity against mouse L1210 cells in presence of 20 uM 2'-deoxyuridine
    Cytostatic activity against mouse L1210 cells in presence of 20 uM 2'-deoxyuridine
    		[PMID: 21330014]
    L1210 IC50
    0.72 μM
    Compound: 5-FUrd
    Cytostatic activity against mouse L1210 cells in presence of 500 uM uridine
    Cytostatic activity against mouse L1210 cells in presence of 500 uM uridine
    		[PMID: 21330014]
    L1210 IC50
    1.2 μM
    Compound: 5-FUrd
    Cytostatic activity against mouse L1210 cells in presence of 20 uM thymidine
    Cytostatic activity against mouse L1210 cells in presence of 20 uM thymidine
    		[PMID: 21330014]
    L1210 IC50
    > 10-4 M
    Compound: 5-FdUrd
    Inhibition of L1210 leukemic cell growth in mice 1 hr after exposure to 5*10e-8 M of compound
    Inhibition of L1210 leukemic cell growth in mice 1 hr after exposure to 5*10e-8 M of compound
    		[PMID: 2704026]
    L1210 IC50
    5.0 x 10-9 M
    Compound: 2
    Concentration required for the 50% inhibition of growth of L1210 mouse leukemia cells in culture
    Concentration required for the 50% inhibition of growth of L1210 mouse leukemia cells in culture
    		[PMID: 7120290]
    L1210 IC50
    3 x 10-9 M
    Compound: 5-FdUrd
    In vitro inhibitory growth activity against L1210 leukemic cells by incubating 5 x 10E4 cells/mL for 48 hour in 2 mL of media.
    In vitro inhibitory growth activity against L1210 leukemic cells by incubating 5 x 10E4 cells/mL for 48 hour in 2 mL of media.
    		[PMID: 2704026]
    MKN-28 IC50
    0.028 μM
    Compound: FUrd (5-fluorouridine)
    Compound was tested for its inhibitory effect on the growth of MKN-28 tumor cell line from stomach.
    Compound was tested for its inhibitory effect on the growth of MKN-28 tumor cell line from stomach.
    		10.1016/0960-894X(96)00339-3
    MKN-45 IC50
    0.026 μM
    Compound: FUrd (5-fluorouridine)
    Compound was tested for its inhibitory effect on the growth of MKN-45 tumor cell line from stomach.
    Compound was tested for its inhibitory effect on the growth of MKN-45 tumor cell line from stomach.
    		10.1016/0960-894X(96)00339-3
    MOLT-3 IC50
    < 0.01 μg/mL
    Compound: 5-F-Urd
    In vitro antitumor activity in MOLT-3 cell lines
    In vitro antitumor activity in MOLT-3 cell lines
    		[PMID: 8648611]
    NUGC-3 IC50
    0.022 μM
    Compound: FUrd (5-fluorouridine)
    Compound was tested for its inhibitory effect on the growth of NUGC-3 tumor cell line from stomach.
    Compound was tested for its inhibitory effect on the growth of NUGC-3 tumor cell line from stomach.
    		10.1016/0960-894X(96)00339-3
    PANC-1 IC50
    11 μM
    Compound: FUrd (5-fluorouridine)
    Compound was tested for its inhibitory effect on the growth of PANC-1 tumor cell line from pancreas.
    Compound was tested for its inhibitory effect on the growth of PANC-1 tumor cell line from pancreas.
    		10.1016/0960-894X(96)00339-3
    PHA-Ly IC50
    < 0.1 μg/mL
    Compound: 5-F-Urd
    IIn vitro antitumor activity in PHA-Ly cell lines
    IIn vitro antitumor activity in PHA-Ly cell lines
    		[PMID: 8648611]
    SNU-C2A IC50
    0.045 μM
    Compound: FUrd (5-fluorouridine)
    Compound was tested for its inhibitory effect on the growth of SUN-C2A tumor cell line from colon.
    Compound was tested for its inhibitory effect on the growth of SUN-C2A tumor cell line from colon.
    		10.1016/0960-894X(96)00339-3
    SW48 IC50
    0.016 μM
    Compound: FUrd (5-fluorouridine)
    Compound was tested for its inhibitory effect on the growth of SW-48 tumor cell line from colon.
    Compound was tested for its inhibitory effect on the growth of SW-48 tumor cell line from colon.
    		10.1016/0960-894X(96)00339-3
    T-24 IC50
    0.014 μM
    Compound: FUrd (5-fluorouridine)
    Compound was tested for its inhibitory effect on the growth of T24 tumor cell line from bladder.
    Compound was tested for its inhibitory effect on the growth of T24 tumor cell line from bladder.
    		10.1016/0960-894X(96)00339-3
    U-937 IC50
    < 0.01 μg/mL
    Compound: 5-F-Urd
    In vitro antitumor activity in U-937 cell lines
    In vitro antitumor activity in U-937 cell lines
    		[PMID: 8648611]
    In Vitro

    5-Fluorouridine (167 μM; 24-96 h) inhibits MKN45 cells and MKN28 cells in a time-dependent manner[1].
    5-Fluorouridine (10 μM; 24 h; HCT-116 cells) induces apoptosis and increases the percentage of apoptotic cells. 5-Fluorouridine upregulates the expression of 33 genes, including a group of genes as growth factors, cytokines and chemokines (e.g. IL-3, IL -4, B-cell growth factor 1 and stem cell growth factor)[2].
    5-Fluorouridine (10 μM; 8-24 h; HCT-116 cells) incorporates into both total and poly A RNA[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    5-Fluorouridine Related Antibodies
    In Vivo

    5-Fluorouridine (567-1500 mg/kg; i.p.; daily, for 20 d) induce gastrointestinal toxicity in the mice[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Male CBA/J mice (17-20 g; 6-8 weeks of age)[3]
    Dosage: Intraperitoneal injection; daily, 20 days
    Administration: 567, 700, 900, 1100, 1300, and 1500 mg/kg
    Result: 567, 700, 900, 1100, 1300, and 1500 mg/kg
    Clinical Trial
    Molecular Weight

    262.19

    Formula

    C9H11FN2O6
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N2C(NC(C(F)=C2)=O)=O)O1
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, stored under nitrogen

    *In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 100 mg/mL (381.40 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 100 mg/mL (381.40 mM; Need ultrasonic)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.8140 mL 19.0701 mL 38.1403 mL
    5 mM 0.7628 mL 3.8140 mL 7.6281 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (7.93 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (7.93 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 110 mg/mL (419.54 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.97%

    SDS (393 KB)

    COA (253 KB) HNMR (254 KB) RP-HPLC (240 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O 1 mM 3.8140 mL 19.0701 mL 38.1403 mL 95.3507 mL
    5 mM 0.7628 mL 3.8140 mL 7.6281 mL 19.0701 mL
    10 mM 0.3814 mL 1.9070 mL 3.8140 mL 9.5351 mL
    15 mM 0.2543 mL 1.2713 mL 2.5427 mL 6.3567 mL
    20 mM 0.1907 mL 0.9535 mL 1.9070 mL 4.7675 mL
    25 mM 0.1526 mL 0.7628 mL 1.5256 mL 3.8140 mL
    30 mM 0.1271 mL 0.6357 mL 1.2713 mL 3.1784 mL
    40 mM 0.0954 mL 0.4768 mL 0.9535 mL 2.3838 mL
    50 mM 0.0763 mL 0.3814 mL 0.7628 mL 1.9070 mL
    60 mM 0.0636 mL 0.3178 mL 0.6357 mL 1.5892 mL
    80 mM 0.0477 mL 0.2384 mL 0.4768 mL 1.1919 mL
    100 mM 0.0381 mL 0.1907 mL 0.3814 mL 0.9535 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    5-Fluorouridine Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    5-Fluorouridine316-46-1Drug MetaboliteDNA/RNA SynthesisApoptosisRNAribozyme self-cleavageantiproliferativeHCT-116 cellsInhibitorinhibitorinhibit

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