2. Apoptosis
  3. Bcl-2 Family
  4. BT2

BT2 is a BCKDC kinase (BDK) inhibitor with an IC50 of 3.19 μM. BT2 binding to BDK triggers helix movements in the N-terminal domain, resulting in the dissociation of BDK from the branched-chain α-ketoacid dehydrogenase complex (BCKDC). BT2 (compound 4) is also a potent and selective Mcl-1 inhibitor with a Ki value of 59 μM.

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CAS 番号 : 34576-94-8

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10 mM * 1 mL in DMSO
ready for reconstitution
 USD 33 在庫あり
Solution
10 mM * 1 mL in DMSO USD 33 在庫あり
Solid
5 mg $30 在庫あり
10 mg $50 在庫あり
25 mg $75 在庫あり
50 mg $90 在庫あり
100 mg $145 在庫あり
500 mg $360 在庫あり
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カスタマーレビュー

Based on 9 publication(s) in Google Scholar

BT2 関連抗体

Top Publications Citing Use of Products

    BT2 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Aug 11:e11081.  [Abstract]

    BT2 (2 μM; 1-4 days) treatment decreased the viability of BCKDK-overexpressing Caki-2 and ACHN cells. CCK-8 assay detecting cell viability after BCKDK overexpression in RCC cells.

    BT2 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Aug 11:e11081.  [Abstract]

    CCK-8 assay assessing the cell viability of 786-O and 769-P cells treated with BT2 (1, 2, 4, 8 μM; 1-4 days).

    BT2 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Aug 11:e11081.  [Abstract]

    786‐O cells were treated with 0, 2, and 4 μM BT2 for 24 h, followed by 2 μM DOX or DMSO treatment for 12 h. The effect of DOX-mediated cell death and apoptosis was analyzed.

    BT2 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Aug 11:e11081.  [Abstract]

    Apoptosis of RCC cells treated with BT2 (2, 4 μM; 24 h) at 780-O and 769-P cells was detected by flow cytometry.

    BT2 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2024 Oct 10;15(10):736.  [Abstract]

    Phosphorylation level of BCKDHA in primary mouse hepatocytes incubated with 200 μM BT2 for 1 h.

    BT2 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2024 Oct 10;15(10):736.  [Abstract]

    Primary mouse hepatocytes were incubated with 200 μM BT2 and 100 μM 8-Br-cAMP in glucose-free DMEM containing gluconeogenic substrates (1 mM sodium pyruvate and 10 mM sodium lactate) for 24 h. The cell culture supernatants were collected to measure glucose content.

    Bcl-2 Family アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Bcl-2 Bcl-xL Bcl-W Mcl-1 Bfl-1 Bcl-B Bax Bak Bim BNIP3 Bad

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    • 純度とドキュメンテーション

    • 参考文献

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    製品説明

    BT2 is a BCKDC kinase (BDK) inhibitor with an IC50 of 3.19 μM. BT2 binding to BDK triggers helix movements in the N-terminal domain, resulting in the dissociation of BDK from the branched-chain α-ketoacid dehydrogenase complex (BCKDC)[1]. BT2 (compound 4) is also a potent and selective Mcl-1 inhibitor with a Ki value of 59 μM[2].

    IC50 & Target[1][2]

    BDK

    3.19 μM (IC50)

    Mcl-1

    59 μM (Ki)

    体内実験

    BT2 (20 mg/kg/day; intraperitoneal injection; daily; for 7 days; C57BL/6J male mice) treatment robustly enhances BCKDC activity in the heart (12.3-fold) compared with the vehicle-treated animals. Less activation is obtained in muscle and kidney at 3.6- and 3.8-fold, respectively. The -fold activation of BCKDC activity in the above tissues correlates with decreased phosphorylation in heart, muscle, and kidney after the long term BT2 treatment. BT2 treatment reduces the protein levels of BDK in kidneys and heart[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: C57BL/6J male mice (8-10-week-old)[1]
    Dosage: 20 mg/kg/day
    Administration: Intraperitoneal injection; daily; for 1 week
    Result: BCKDC activity was robustly (12.3-fold) enhanced in the heart compared with the vehicle-treated animals. Less activation was obtained in muscle and kidney at 3.6- and 3.8-fold, respectively. The protein levels of BDK in kidneys and heart were reduced to averages of 39 and 24%, respectively.
    分子量

    247.10

    分子式

    C9H4Cl2O2S
    CAS 番号
    Appearance

    Solid

    Color

    White to light yellow

    SMILES

    O=C(O)C1=C(C2=CC=C(C=C2S1)Cl)Cl
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Powder -20°C 3 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : 62.5 mg/mL (252.93 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 4.0469 mL 20.2347 mL 40.4694 mL
    5 mM 0.8094 mL 4.0469 mL 8.0939 mL
    10 mM 0.4047 mL 2.0235 mL 4.0469 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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    一般には略語で表示されます：C1V1 = C2V2

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    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (8.42 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 2.08 mg/mL (8.42 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
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    +
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    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 99.81%

    COA (250 KB) HNMR (141 KB) RP-HPLC (246 KB) LCMS (388 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 4.0469 mL 20.2347 mL 40.4694 mL 101.1736 mL
    5 mM 0.8094 mL 4.0469 mL 8.0939 mL 20.2347 mL
    10 mM 0.4047 mL 2.0235 mL 4.0469 mL 10.1174 mL
    15 mM 0.2698 mL 1.3490 mL 2.6980 mL 6.7449 mL
    20 mM 0.2023 mL 1.0117 mL 2.0235 mL 5.0587 mL
    25 mM 0.1619 mL 0.8094 mL 1.6188 mL 4.0469 mL
    30 mM 0.1349 mL 0.6745 mL 1.3490 mL 3.3725 mL
    40 mM 0.1012 mL 0.5059 mL 1.0117 mL 2.5293 mL
    50 mM 0.0809 mL 0.4047 mL 0.8094 mL 2.0235 mL
    60 mM 0.0674 mL 0.3372 mL 0.6745 mL 1.6862 mL
    80 mM 0.0506 mL 0.2529 mL 0.5059 mL 1.2647 mL
    100 mM 0.0405 mL 0.2023 mL 0.4047 mL 1.0117 mL
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    BT2 Related Classifications

    • Molarity Calculator

    • Dilution Calculator

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    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
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    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    BT234576-94-8BT 2BT-2Bcl-2 FamilyInhibitorinhibitorinhibit

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    製品名:
    BT2
    製品番号:
    HY-114855
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