2. Metabolic Enzyme/Protease GPCR/G Protein
  3. Angiotensin-converting Enzyme (ACE) Endothelin Receptor
  4. H-Trp-Phe-OH

H-Trp-Phe-OH (Trp-Phe) is an ACE inhibitor and endothelial function regulator, with an ACE IC50 of 0.318 mg/mL. H-Trp-Phe-OH increases nitric oxide concentration, reduces endothelin-1 (ET-1) levels, and reverses norepinephrine-mediated endothelial cell dysfunction. H-Trp-Phe-OH exhibits antihypertensive activity. H-Trp-Phe-OH can be used in studies related to hypertension and atherosclerosis.

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H-Trp-Phe-OH

H-Trp-Phe-OH Chemical Structure

CAS No. : 6686-02-8

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H-Trp-Phe-OH Related Antibodies

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Description

H-Trp-Phe-OH (Trp-Phe) is an ACE inhibitor and endothelial function regulator, with an ACE IC50 of 0.318 mg/mL. H-Trp-Phe-OH increases nitric oxide concentration, reduces endothelin-1 (ET-1) levels, and reverses norepinephrine-mediated endothelial cell dysfunction. H-Trp-Phe-OH exhibits antihypertensive activity. H-Trp-Phe-OH can be used in studies related to hypertension and atherosclerosis[1].

In Vitro

H-Trp-Phe-OH (Trp-Phe) (200-300 μM; 24 h) does not cause significant cytotoxicity in HUVECs[1].
H-Trp-Phe-OH (Trp-Phe) (50-200 μM; 24 h) increases NO production in HUVECs in a concentration-dependent manner (up to 2.35 μmol/gprot at 200 μM over 24 h) and reverses norepinephrine-induced reductions in NO production in HUVECs[1].
H-Trp-Phe-OH (Trp-Phe) (50-200 μM; 24 h) decreases ET-1 production in HUVECs and reverses norepinephrine-induced increases in ET-1 production in HUVECs, with significant activity observed at 50, 100, and 200 μM over 24 h[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

H-Trp-Phe-OH Related Antibodies

ELISA Assay[1]

Cell Line: human umbilical vein endothelial cells (HUVECs)
Concentration: 50 μM; 100 μM; 200 μM; 200 μM (in the presence of 0.5 μM norepinephrine)
Incubation Time: 24 h
Result: Significantly decreased ET-1 production relative to blank control HUVECs.
Significantly reduced the norepinephrine-induced increase in ET-1 production in HUVECs, with the effect of 200 μM Trp-Phe being greater than that of other tested peptides.
Molecular Weight

351.40

Formula

C20H21N3O3
CAS No.
Appearance

Solid

Color

White to off-white

Sequence

H-Trp-Phe-OH
Sequence Shortening

WF
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (284.58 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.8458 mL 14.2288 mL 28.4576 mL
5 mM 0.5692 mL 2.8458 mL 5.6915 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.8458 mL 14.2288 mL 28.4576 mL 71.1440 mL
5 mM 0.5692 mL 2.8458 mL 5.6915 mL 14.2288 mL
10 mM 0.2846 mL 1.4229 mL 2.8458 mL 7.1144 mL
15 mM 0.1897 mL 0.9486 mL 1.8972 mL 4.7429 mL
20 mM 0.1423 mL 0.7114 mL 1.4229 mL 3.5572 mL
25 mM 0.1138 mL 0.5692 mL 1.1383 mL 2.8458 mL
30 mM 0.0949 mL 0.4743 mL 0.9486 mL 2.3715 mL
40 mM 0.0711 mL 0.3557 mL 0.7114 mL 1.7786 mL
50 mM 0.0569 mL 0.2846 mL 0.5692 mL 1.4229 mL
60 mM 0.0474 mL 0.2371 mL 0.4743 mL 1.1857 mL
80 mM 0.0356 mL 0.1779 mL 0.3557 mL 0.8893 mL
100 mM 0.0285 mL 0.1423 mL 0.2846 mL 0.7114 mL
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H-Trp-Phe-OH Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

H-Trp-Phe-OH6686-02-8Trp-PheAngiotensin-converting Enzyme (ACE)Endothelin Receptorendothelial functionHUVECsatherosclerosisACEnitric oxidehypertensionnorepinephrineendothelin-1endothelial cell dysfunctionInhibitorinhibitorinhibit

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