2. MAPK/ERK Pathway
  3. JNK
  4. Halicin

Halicin (SU3327) is a potent, selective and substrate-competitive JNK inhibitor with an IC50 of 0.7 μM. Halicin also inhibits protein-protein interactions between JNK and JNK Interacting Protein (JIP) with an IC50 of 239 nM. Halicin shows less active against p38α and Akt kinase.

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Halicin

Halicin 構造式

CAS 番号 : 40045-50-9

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 82 在庫あり
Solution
10 mM * 1 mL in DMSO USD 82 在庫あり
Solid
5 mg $75 在庫あり
10 mg $120 在庫あり
50 mg $400 在庫あり
100 mg $600 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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Based on 2 publication(s) in Google Scholar

Other Forms of Halicin:

Halicin 関連抗体

Top Publications Citing Use of Products

JNK アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示
JNK1 JNK2 JNK3 JNK

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製品説明

Halicin (SU3327) is a potent, selective and substrate-competitive JNK inhibitor with an IC50 of 0.7 μM. Halicin also inhibits protein-protein interactions between JNK and JNK Interacting Protein (JIP) with an IC50 of 239 nM. Halicin shows less active against p38α and Akt kinase[1][2].

IC50 & Target

IC50: 0.7 μM (JNK); 239 nM (JNK-JIP interactions)[1]
Cellular Effect
Cell Line Type Value Description References
HeLa EC50
6.23 3
Compound: 9
Inhibition of TNF-alpha-stimulated JNK phosphorylation expressed in HeLa cells by time-resolved FRET assay
Inhibition of TNF-alpha-stimulated JNK phosphorylation expressed in HeLa cells by time-resolved FRET assay
[PMID: 19271755]
HeLa EC50
6.23 3
Compound: 9
Inhibition of TNF-alpha-stimulated JNK phosphorylation expressed in HeLa cells by time-resolved FRET assay
Inhibition of TNF-alpha-stimulated JNK phosphorylation expressed in HeLa cells by time-resolved FRET assay
[PMID: 19271755]
HeLa EC50
6.23 3
Compound: 9
Inhibition of TNF-alpha-stimulated JNK phosphorylation expressed in HeLa cells by time-resolved FRET assay
Inhibition of TNF-alpha-stimulated JNK phosphorylation expressed in HeLa cells by time-resolved FRET assay
[PMID: 19271755]
体外実験

Halicin (compound 9) is able to inhibit TNF-α stimulated phosphorylation of c-Jun in HeLa cells (EC50 = 6.23 μM)[1].
Halicin (25 nM) pretreatment of? human-derived cerebral microvascular endothelial cells (hCMEC/D3) effectively reduces LPS-induced polymorphonuclear leukocytes (PMN) rolling/adhesion to hCMEC/D3, prevents activation of AP-1, and significantly reduces expression of VCAM-1[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Halicin 関連抗体
体内実験

Halicin (Compound 9; 25 mg/kg; intraperitoneal injection; male BKS.Cg-+Leprdb/+Leprdb/OlaHsd db/db mice) treatment possesses the ability to restore insulin sensitivity in mice models of diabetes[1].
Halicin (Compound 9) has favorable microsomal and plasma stability (T1/2 = 27 min)[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male BKS.Cg-+Leprdb/+Leprdb/OlaHsd db/db mice (11-week-old ) injected with insulin[1]
Dosage: 25 mg/kg
Administration: Intraperitoneal injection
Result: Resulted in a statistically significant reduction in blood glucose levels.
分子量

261.30

分子式

C5H3N5O2S3
CAS 番号
Appearance

Solid

Color

Light yellow to yellow

SMILES

NC1=NN=C(S1)SC2=NC=C([N+]([O-])=O)S2
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
溶剤 & 溶解度
体外: 

DMSO : 62.5 mg/mL (239.19 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.8270 mL 19.1351 mL 38.2702 mL
5 mM 0.7654 mL 3.8270 mL 7.6540 mL
10 mM 0.3827 mL 1.9135 mL 3.8270 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

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体積 （終了）

V2

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (7.96 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (7.96 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

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g

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(per animal)

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
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%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション

純度: 98.92%

COA (248 KB) HNMR (208 KB) LCMS (94 KB)

取扱説明書 (2659 KB)
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.8270 mL 19.1351 mL 38.2702 mL 95.6755 mL
5 mM 0.7654 mL 3.8270 mL 7.6540 mL 19.1351 mL
10 mM 0.3827 mL 1.9135 mL 3.8270 mL 9.5675 mL
15 mM 0.2551 mL 1.2757 mL 2.5513 mL 6.3784 mL
20 mM 0.1914 mL 0.9568 mL 1.9135 mL 4.7838 mL
25 mM 0.1531 mL 0.7654 mL 1.5308 mL 3.8270 mL
30 mM 0.1276 mL 0.6378 mL 1.2757 mL 3.1892 mL
40 mM 0.0957 mL 0.4784 mL 0.9568 mL 2.3919 mL
50 mM 0.0765 mL 0.3827 mL 0.7654 mL 1.9135 mL
60 mM 0.0638 mL 0.3189 mL 0.6378 mL 1.5946 mL
80 mM 0.0478 mL 0.2392 mL 0.4784 mL 1.1959 mL
100 mM 0.0383 mL 0.1914 mL 0.3827 mL 0.9568 mL
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Halicin Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Halicin40045-50-9SU3327SU 3327SU-3327JNKc-Jun N-terminal kinaseThiadiazolesubstrate-competitiveJIPp38αprotein-proteinTNF-αphosphorylationinsulinPPIInhibitorinhibitorinhibit

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製品名:
Halicin
製品番号:
HY-107597
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