Hydroxocobalamin  (Synonyms: Vitamin B12a)

Hydroxocobalamin (Vitamin B12a) is a cyanide antidote. Hydroxocobalamin can decrease the cell and plasma cyanide concentrations and increase survival rate in acute cyanide poisoning. Hydroxocobalamin can improve newborn Cbl-C defect-induced metabolic profile and neurocognitive outcome. Hydroxocobalamin can normalize Vitamin B₁₂ deficiency. Hydroxocobalamin can induce acute kidney injury. Hydroxocobalamin can be used for the researches of metabolic and neurological disease^{[1][2][3][4][5][6]}.

Hydroxocobalamin (1 mM, 5 mins) recovers sodium cyanide-induced inotropy decrease in cardiac papillary muscle^[1].
Hydroxocobalamin (500 μM, 10 mins) decreases intracellular cyanide in fibroblasts^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Hydroxocobalamin (150 mg/kg, i.v.) maintains higher systolic blood pressure in both controlled and uncontrolled hemorrhage swine models^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1346.36

C₆₂H₈₉CoN₁₃O₁₅P

13422-51-0

Solid

Purple to reddish brown

C[C@@]12[N]([Co+3]34([N]5=C[N@@]([C@@H](O[C@H](CO)[C@H]6OP7([O-])=O)[C@@H]6O)C8=C5C=C(C)C(C)=C8)([OH-])[N-]9C2[C@H](CC(N)=O)[C@@](C)(CCC(NC[C@H](C)O7)=O)C9=C(C)C([C@@H](CCC(N)=O)C%10(C)C)=[N]3C%10=CC%11=[N]4C([C@](CC(N)=O)(C)[C@@H]%11CCC(N)=O)=C%12C)=C%12[C@@H](CCC(N)=O)[C@]1(C)CC(N)=O

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. Thompson JP, et al. Hydroxocobalamin in cyanide poisoning. Clin Toxicol (Phila). 2012;50(10):875-885.  [Content Brief]

  [2]. Paredes RM, et al. Comparison of hydroxocobalamin with other resuscitative fluids in volume-controlled and uncontrolled hemorrhage models in swine ( Sus-scrofa ). J Trauma Acute Care Surg. 2023;95(2S Suppl 1):S120-S128.  [Content Brief]

  [3]. Olivieri G, et al. Improved biochemical and neurodevelopmental profiles with high-dose hydroxocobalamin therapy in cobalamin C defect. J Inherit Metab Dis. 2025;48(1):e12787.  [Content Brief]

  [4]. Dépret F, et al. Association between hydroxocobalamin administration and acute kidney injury after smoke inhalation: a multicenter retrospective study. Crit Care. 2019;23(1):421. Published 2019 Dec 23.  [Content Brief]

  [5]. Bruins Slot, et al. Normalization of plasma vitamin B12 concentration by intranasal hydroxocobalamin in vitamin B12-deficient patients. Gastroenterology. 1997 Aug;113(2):430-3.  [Content Brief]

  [6]. Shepherd G, et al. Role of hydroxocobalamin in acute cyanide poisoning. Ann Pharmacother. 2008;42(5):661-669.  [Content Brief]