2. Metabolic Enzyme/Protease
  3. Endogenous Metabolite
  4. L-Leucyl-L-Leucine methyl ester hydrochloride

L-Leucyl-L-Leucine methyl ester hydrochloride  (Synonyms: LLOMe hydrochloride; Leu-Leu methyl ester hydrochloride; H-Leu-Leu-OMe hydrochloride)

製品番号: HY-129905 純度: 99.09%
COA 取扱説明書 Technical Support

L-Leucyl-L-Leucine methyl ester (LLOMe) hydrochloride, a dipeptide condensation product of L-leucine methyl ester generated by human monocytes or polymorphonuclear leukocytes, selectively eliminates lymphocytes with cytotoxic potential. L-Leucyl-L-Leucine methyl ester hydrochloride also can induce endolysosomal pathway stress.

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CAS 番号 : 6491-83-4

容量 価格（税別） 在庫状況 数量
>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 55 在庫あり
Solution
10 mM * 1 mL in DMSO USD 55 在庫あり
Solid
100 mg $50 在庫あり
250 mg $100 在庫あり
500 mg   お問い合わせ  
1 g   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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カスタマーレビュー

Based on 18 publication(s) in Google Scholar

Other Forms of L-Leucyl-L-Leucine methyl ester hydrochloride:

L-Leucyl-L-Leucine methyl ester hydrochloride 関連抗体

Top Publications Citing Use of Products

    L-Leucyl-L-Leucine methyl ester hydrochloride purchased from MedChemExpress. Usage Cited in: EMBO J. 2025 May;44(9):2596-2619.  [Abstract]

    S-palmitoylation of HA-ATG2A detected by ABE assay in HEK293T cells treated with 1 mM LLOMe for indicated times.

    L-Leucyl-L-Leucine methyl ester hydrochloride purchased from MedChemExpress. Usage Cited in: J Cell Biol. 2025 Nov 3;224(11):e202404018.  [Abstract]

    U2OS cells were transfected with HaloTag-LRRK2 for 36 h. Cells were then treated with DMSO, LLOME (1 mM), or LLOME+MLi2 for 2 h before fixation. Cells were then stained for RILPL1 and LAMP2.

    L-Leucyl-L-Leucine methyl ester hydrochloride purchased from MedChemExpress. Usage Cited in: J Cell Biol. 2025 Nov 3;224(11):e202404018.  [Abstract]

    Quantification of the RILPL1 lysosomes per cell. Cells were treated with DMSO, LLOME (1 mM), or LLOME+MLi2 for 2 h.

    L-Leucyl-L-Leucine methyl ester hydrochloride purchased from MedChemExpress. Usage Cited in: J Cell Biol. 2025 Nov 3;224(11):e202404018.  [Abstract]

    HEK293FT cells were transfected with 3xflag-LRRK2 and mCherry-RILPL1 for 24 h. Cells were treated or not with LLOME (1 mM) for 2 h, and lysates were subjected to immunoprecipitation with anti-RFP antibodies. RFP, LRRK2, and pT73-RAB10 were blotted.

    L-Leucyl-L-Leucine methyl ester hydrochloride purchased from MedChemExpress. Usage Cited in: J Cell Biol. 2025 Nov 3;224(11):e202404018.  [Abstract]

    HEK293T cells stably expressing GFP-LRRK2 and TMEM192-3xHA were seeded and treated with DMSO, LLOME (1 mM), or LLOME + MLi2 24 h later. Lysosomes were purified with anti-HA beads following the LYSO-IP technique. Lysosomes were then lysed, and their content was analyzed via immunoblotting.

    Endogenous Metabolite アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Human Endogenous Metabolite Microbial Metabolite

    • 生物活性

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    L-Leucyl-L-Leucine methyl ester (LLOMe) hydrochloride, a dipeptide condensation product of L-leucine methyl ester generated by human monocytes or polymorphonuclear leukocytes, selectively eliminates lymphocytes with cytotoxic potential. L-Leucyl-L-Leucine methyl ester hydrochloride also can induce endolysosomal pathway stress[1][2][3].

    体外実験

    L-Leucyl-L-Leucine methyl ester (1 mM; 0.5-2 h) enhances LRRK2-mediated Rab10 and Rab12 phosphorylation in MEFs and A549 cells[3].
    L-Leucyl-L-Leucine methyl ester (10-250 μM; 15 min) is converted to a CCI3COOH-insoluble product by CD4- lymphocytes[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    L-Leucyl-L-Leucine methyl ester hydrochloride 関連抗体
    分子量

    294.82

    分子式

    C13H27ClN2O3
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    CC(C)C[C@@H](C(OC)=O)NC([C@H](CC(C)C)N)=O.[H]Cl
    Structure Classification
    Initial Source
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
    溶剤 & 溶解度
    体外: 

    DMSO : 250 mg/mL (847.98 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.3919 mL 16.9595 mL 33.9190 mL
    5 mM 0.6784 mL 3.3919 mL 6.7838 mL
    10 mM 0.3392 mL 1.6960 mL 3.3919 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

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    体積 （終了）

    V2

    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (7.06 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (7.06 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 99.09%

    COA (247 KB) HNMR (249 KB) RP-HPLC (223 KB) MS (73 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.3919 mL 16.9595 mL 33.9190 mL 84.7975 mL
    5 mM 0.6784 mL 3.3919 mL 6.7838 mL 16.9595 mL
    10 mM 0.3392 mL 1.6960 mL 3.3919 mL 8.4798 mL
    15 mM 0.2261 mL 1.1306 mL 2.2613 mL 5.6532 mL
    20 mM 0.1696 mL 0.8480 mL 1.6960 mL 4.2399 mL
    25 mM 0.1357 mL 0.6784 mL 1.3568 mL 3.3919 mL
    30 mM 0.1131 mL 0.5653 mL 1.1306 mL 2.8266 mL
    40 mM 0.0848 mL 0.4240 mL 0.8480 mL 2.1199 mL
    50 mM 0.0678 mL 0.3392 mL 0.6784 mL 1.6960 mL
    60 mM 0.0565 mL 0.2827 mL 0.5653 mL 1.4133 mL
    80 mM 0.0424 mL 0.2120 mL 0.4240 mL 1.0600 mL
    100 mM 0.0339 mL 0.1696 mL 0.3392 mL 0.8480 mL
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    L-Leucyl-L-Leucine methyl ester hydrochloride Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    L-Leucyl-L-Leucine methyl ester6491-83-4LLOMeLeu-Leu methyl esterH-Leu-Leu-OMeEndogenous MetabolitemonocytespolymorphonuclearleukocyteslymphocytescytotoxicendolysosomalpathwaystressInhibitorinhibitorinhibit

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    製品名:
    L-Leucyl-L-Leucine methyl ester hydrochloride
    製品番号:
    HY-129905
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