N-trans-Feruloyltyramine (Synonyms: N-feruloyltyramine; Moupinamide)

Name: N-trans-Feruloyltyramine
Brand: MedChemExpress
SKU: HY-N2410
Rating: 4.5 (0 reviews)

Cat. No.: HY-N2410 Purity: 99.90%: Data Sheet
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N-trans-Feruloyltyramine (N-feruloyltyramine), an alkaloid, is a potent antioxidant. N-trans-Feruloyltyramine improves H₂O₂-induced intracellular ROS generation and decreases apoptosis. N-trans-Feruloyltyramine has the potential for oxidative stress-related neurodegenerative conditions and cancer research.

For research use only. We do not sell to patients.

N-trans-Feruloyltyramine Chemical Structure

CAS No. : 66648-43-9

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Other Forms of N-trans-Feruloyltyramine:

N-trans-Feruloyltyramine (Standard) Get quote

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Description

Cellular Effect

Cell Line	Type	Value	Description	References
A549	ED₅₀	13.35 6 Compound: 6	Cytotoxicity against human A549 cells after 7 days Cytotoxicity against human A549 cells after 7 days	[PMID: 1593281]
A549	IC₅₀	>40 3 Compound: 24	Antiproliferative activity against human A549 cells after 3 days by SRB assay Antiproliferative activity against human A549 cells after 3 days by SRB assay	[PMID: 25241925]
A549	IC₅₀	>30 3 Compound: 7	Cytotoxicity against human A549 cells by SRB assay Cytotoxicity against human A549 cells by SRB assay	[PMID: 22951040]
BV-2	IC₅₀	17.36 3 Compound: 7	Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production treated 30 mins before LPS stimulation measured after 24 hrs by Griess reaction Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production treated 30 mins before LPS stimulation measured after 24 hrs by Griess reaction	[PMID: 22951040]
BV-2	IC₅₀	18 3 Compound: 21	Antineuroinflammatory activity against mouse BV2 cells assessed as inhibition of LPS-induced NO production incubated for 20 hrs by Griess assay Antineuroinflammatory activity against mouse BV2 cells assessed as inhibition of LPS-induced NO production incubated for 20 hrs by Griess assay	[PMID: 33822610]
DU-145	IC₅₀	>40 3 Compound: 24	Antiproliferative activity against human DU145 cells after 3 days by SRB assay Antiproliferative activity against human DU145 cells after 3 days by SRB assay	[PMID: 25241925]
Erythrocyte	IC₅₀	1.33 2 Compound: 25	Anticomplement activity in rabbit erythrocytes assessed as concentration required for 50% hemolytic inhibition by alternative pathway pretreated for 10 mins with normal human serum followed by erythrocyte addition measured after 30 mins by spectrophotomet Anticomplement activity in rabbit erythrocytes assessed as concentration required for 50% hemolytic inhibition by alternative pathway pretreated for 10 mins with normal human serum followed by erythrocyte addition measured after 30 mins by spectrophotomet	[PMID: 29631958]
Erythrocyte	IC₅₀	0.88 2 Compound: 25	Anticomplement activity in sheep erythrocytes assessed as concentration required for 50% hemolytic inhibition by classic pathway pretreated for 10 mins with guinea pig serum followed by erythrocyte addition measured after 30 mins by spectrophotometeric me Anticomplement activity in sheep erythrocytes assessed as concentration required for 50% hemolytic inhibition by classic pathway pretreated for 10 mins with guinea pig serum followed by erythrocyte addition measured after 30 mins by spectrophotometeric me	[PMID: 29631958]
GES1	IC₅₀	>150 3 Compound: 14	Antiproliferative activity against human GES1 cells assessed as reduction in cell viability after 24 hrs by MTT assay Antiproliferative activity against human GES1 cells assessed as reduction in cell viability after 24 hrs by MTT assay	[PMID: 35286954]
HCT-15	IC₅₀	>30 3 Compound: 7	Cytotoxicity against human HCT15 cells by SRB assay Cytotoxicity against human HCT15 cells by SRB assay	[PMID: 22951040]
HepG2	IC₅₀	6.6 6 Compound: 6	Cytotoxicity against human HepG2 cells by MTT assay Cytotoxicity against human HepG2 cells by MTT assay	[PMID: 11374955]
HepG2 2.2.15	IC₅₀	1.9 6 Compound: 6	Cytotoxicity against human HepG2(2.2.15) cells by MTT assay Cytotoxicity against human HepG2(2.2.15) cells by MTT assay	[PMID: 11374955]
HGC-27	IC₅₀	84.5 3 Compound: 14	Antiproliferative activity against human HGC-27 cells assessed as reduction in cell viability after 24 hrs by MTT assay Antiproliferative activity against human HGC-27 cells assessed as reduction in cell viability after 24 hrs by MTT assay	[PMID: 35286954]
HT-29	ED₅₀	23.58 6 Compound: 6	Cytotoxicity against human HT-29 cells after 7 days Cytotoxicity against human HT-29 cells after 7 days	[PMID: 1593281]
HT-29	IC₅₀	>20 3 Compound: 17	Cytotoxicity against human HT-29 cells assessed as reduction in cell growth incubated for 72 hrs Cytotoxicity against human HT-29 cells assessed as reduction in cell growth incubated for 72 hrs	[PMID: 26147490]
KB	IC₅₀	>40 3 Compound: 24	Antiproliferative activity against human KB cells after 3 days by SRB assay Antiproliferative activity against human KB cells after 3 days by SRB assay	[PMID: 25241925]
KB	IC₅₀	>47.8 3 Compound: 12	Cytotoxicity against human KB cells by sulforhodamine B assay Cytotoxicity against human KB cells by sulforhodamine B assay	[PMID: 26928423]
LNCaP	IC₅₀	>100 3 Compound: 5	Cytotoxicity against human LNCAP cells after 48 hrs by MTT assay Cytotoxicity against human LNCAP cells after 48 hrs by MTT assay	[PMID: 11858758]
MCF7	ED₅₀	4.76 6 Compound: 6	Cytotoxicity against human MCF7 cells after 7 days Cytotoxicity against human MCF7 cells after 7 days	[PMID: 1593281]
MCF7	IC₅₀	>40 3 Compound: 24	Antiproliferative activity against human MCF7 cells after 3 days by SRB assay Antiproliferative activity against human MCF7 cells after 3 days by SRB assay	[PMID: 25241925]
MDA-MB-231	IC₅₀	>40 3 Compound: 24	Antiproliferative activity against human MDA-MB-231 cells after 3 days by SRB assay Antiproliferative activity against human MDA-MB-231 cells after 3 days by SRB assay	[PMID: 25241925]
MDA-MB-435	IC₅₀	>20 3 Compound: 17	Cytotoxicity against human MDA-MB-435 cells assessed as reduction in cell growth incubated for 72 hrs Cytotoxicity against human MDA-MB-435 cells assessed as reduction in cell growth incubated for 72 hrs	[PMID: 26147490]
MGC-803	IC₅₀	98.3 3 Compound: 14	Antiproliferative activity against human MGC-803 cells assessed as reduction in cell viability after 24 hrs by MTT assay Antiproliferative activity against human MGC-803 cells assessed as reduction in cell viability after 24 hrs by MTT assay	[PMID: 35286954]
RAW264.7	IC₅₀	6.33 3 Compound: 9	Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced PGE2 production preincubated for 1 hr followed by LPS-stimulation and measured after 18 hrs Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced PGE2 production preincubated for 1 hr followed by LPS-stimulation and measured after 18 hrs	[PMID: 31126853]
RAW264.7	IC₅₀	>50 3 Compound: 25	Antiinflammatory activity in mouse RAW264.7 assessed as inhibition of LPS-induced NO production preincubated for 30 mins followed by LPS stimulation measured after 24 hrs by Griess reagent based assay Antiinflammatory activity in mouse RAW264.7 assessed as inhibition of LPS-induced NO production preincubated for 30 mins followed by LPS stimulation measured after 24 hrs by Griess reagent based assay	[PMID: 33939429]
SK-BR-3	IC₅₀	>40 3 Compound: 24	Antiproliferative activity against human SK-BR-3 cells after 3 days by SRB assay Antiproliferative activity against human SK-BR-3 cells after 3 days by SRB assay	[PMID: 25241925]
SK-MEL-2	IC₅₀	>30 3 Compound: 7	Cytotoxicity against human SK-MEL-2 cells by SRB assay Cytotoxicity against human SK-MEL-2 cells by SRB assay	[PMID: 22951040]
SK-OV-3	IC₅₀	>30 3 Compound: 7	Cytotoxicity against human SKOV3 cells by SRB assay Cytotoxicity against human SKOV3 cells by SRB assay	[PMID: 22951040]
Vero	IC₅₀	>47.8 3 Compound: 12	Cytotoxicity against African green monkey Vero cells by sulforhodamine B assay Cytotoxicity against African green monkey Vero cells by sulforhodamine B assay	[PMID: 26928423]
ZR-75-1	IC₅₀	>40 3 Compound: 24	Antiproliferative activity against human ZR-75-1 cells after 3 days by SRB assay Antiproliferative activity against human ZR-75-1 cells after 3 days by SRB assay	[PMID: 25241925]

In Vitro

N-trans-Feruloyltyramine (N-feruloyltyramine; 10-500 µM; 3 hours prior H₂O₂) protects the cells against H₂O₂-induced toxicity^[1].
N-trans-Feruloyltyramine (25-100 µM; 3 hours prior H₂O₂) with 100 µM significantly reduces Bax and activated caspase-3 levels in H₂O₂-treated SK-N-SH cells^[1].
N-trans-Feruloyltyramine significantly improves an H₂O₂-mediated increase in ROS levels^[1].
N-trans-Feruloyltyramine (10-500 µM) does not affect viability of the SK-N-SH cells^[1].
N-trans-Feruloyltyramine (64-320 µM; 24 hours) shows proliferation inhibition on HepG2 cells with IC₅₀ value of 194 µM^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	SK-N-SH cells
Concentration:	10, 25, 50, 100, 150, 250, 500 µM
Incubation Time:	For 3 hours prior H₂O₂
Result:	Protected the cells against H₂O₂ (150 µM)-induced toxicity as determined by a significant higher percentage of viability in cells.

Western Blot Analysis^[1]

Cell Line:	SK-N-SH cells
Concentration:	25, 50, 100 μM
Incubation Time:	For 3 hours prior H₂O₂
Result:	Abolished H₂O₂-induced Bax expression. Significantly decreased activated caspase-3 levels.

Clinical Trial

Molecular Weight

313.35

Formula

C₁₈H₁₉NO₄

CAS No.

66648-43-9

Appearance

Solid

Color

White to off-white

SMILES

O=C(NCCC1=CC=C(O)C=C1)/C=C/C2=CC=C(O)C(OC)=C2

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (319.13 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.1913 mL	15.9566 mL	31.9132 mL
5 mM	0.6383 mL	3.1913 mL	6.3826 mL
10 mM	0.3191 mL	1.5957 mL	3.1913 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.98 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.98 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (7.98 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.90%

Select Batch:

Data Sheet (271 KB) SDS (398 KB)

COA (243 KB) HNMR (277 KB) LCMS (104 KB)

Handling Instructions (2659 KB)

References

[1]. Rungtip Soi-ampornkul, et al. N-trans-feruloyltyramine Protects Human Neuroblastoma SK-N-SH Cell Line Against H2O2-Induced Cytotoxicity. Natural Product CommunicationsVolume 17, Issue 8, August 2022.

[2]. Xudong Gao, et al. Effects of N-trans-feruloyltyramine isolated from laba garlic on antioxidant, cytotoxic activities and H2O2-induced oxidative damage in HepG2 and L02 cells. Food Chem Toxicol. 2019 Aug:130:130-141. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.1913 mL	15.9566 mL	31.9132 mL	79.7830 mL
	5 mM	0.6383 mL	3.1913 mL	6.3826 mL	15.9566 mL
	10 mM	0.3191 mL	1.5957 mL	3.1913 mL	7.9783 mL
	15 mM	0.2128 mL	1.0638 mL	2.1275 mL	5.3189 mL
	20 mM	0.1596 mL	0.7978 mL	1.5957 mL	3.9891 mL
	25 mM	0.1277 mL	0.6383 mL	1.2765 mL	3.1913 mL
	30 mM	0.1064 mL	0.5319 mL	1.0638 mL	2.6594 mL
	40 mM	0.0798 mL	0.3989 mL	0.7978 mL	1.9946 mL
	50 mM	0.0638 mL	0.3191 mL	0.6383 mL	1.5957 mL
	60 mM	0.0532 mL	0.2659 mL	0.5319 mL	1.3297 mL
	80 mM	0.0399 mL	0.1995 mL	0.3989 mL	0.9973 mL
	100 mM	0.0319 mL	0.1596 mL	0.3191 mL	0.7978 mL

N-trans-Feruloyltyramine Related Classifications

Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

N-trans-Feruloyltyramine66648-43-9N-feruloyltyramine MoupinamideReactive Oxygen Species (ROS)ApoptosisalkaloidantioxidantH2O2ROS generationoxidative stressneurodegenerative conditioncancerInhibitorinhibitorinhibit

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N-trans-Feruloyltyramine (Synonyms: N-feruloyltyramine; Moupinamide)

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Complete Stock Solution Preparation Table

N-trans-Feruloyltyramine Related Classifications

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