Rozanolixizumab  (Synonyms: UCB7665)

Rozanolixizumab (UCB7665), a humanized high-affinity anti-human neonatal Fc receptor (FcRn) monoclonal antibody (IgG4P), is used to the research of reducing pathogenic IgG in autoimmune and alloimmune diseases^[1].

Human IgG4 kappa

Human IgG4 (S228P) kappa, Isotype Control

Human

FcRn

Rozanolixizumab binds to human FcRn and cynomolgus monkey FcRn with a similar affinity at both pH 6.0 (K_d 23 pM and 25 pM, human and cynomolgus monkey, respectively) and pH 7.4 (34 pM and 53 pM, human and cynomolgus monkey, respectively. Rozanolixizumab is observed to inhibit the recycling (IC₅₀ 0.41 nM) of human IgG by human FcRn-transfected MDCK cells in a dose-dependent manner. Recycling of IgG was also inhibits in cynomolgus monkey FcRn-transfected MDCK cells (IC₅₀ 0.98nM)^[1].
Rozanolixizumab causes an increase in intracellular IgG AF647^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Intravenous (IV) rozanolixizumab dosing in cynomolgus monkeys demonstrated non-linear pharmacokinetics indicative of target-mediated drug disposition; single IV rozanolixizumab doses (30 mg/kg) in cynomolgus monkeys reduced plasma IgG concentration by 69% by Day 7 post-administration. Daily IV administration of rozanolixizumab (initial 30 mg/kg loading dose; 5 mg/kg daily thereafter) reduced plasma IgG concentrations in all cynomolgus monkeys, with low concentrations maintained throughout the treatment period (42 days)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

2217  [NCBI]

P55899

ELISA, FACS, Functional assay

Unconjugated

The product can be reconstituted/diluted with sterile PBS or saline.

145.242 kDa

1584645-37-3

Liquid

Colorless to light yellow

[Rozanolixizumab]

Shipping with dry ice.

Please refer to the lot-specific COA for specific buffer information.

Please store the product under the recommended conditions in the Certificate of Analysis.

• 
  Human IgG4 kappa

• 
  Immobilized FCRN-B2M Protein, Human (HEK293, His, HY-P70601) can bind Rozanolixizumab. The EC₅₀ for this effect is 2.159 ng/mL.
• 
  Loaded Rozanolixizumab on AHC2 biosensor, can bind FCRN-B2M Protein, Human (Biotinylated, HEK293, His-Avi, HY-P72368) with an affinity constant of 1.430E-10 M as determined in BLI assay.

• [1]. Smith B, et al. Generation and characterization of a high affinity anti-human FcRn antibody, rozanolixizumab, and the effects of different molecular formats on the reduction of plasma IgG concentration. MAbs. 2018;10(7):1111-1130.  [Content Brief]