10,12-Tricosadiynoic acid
Based on 10 publication(s) in Google Scholar
10,12-Tricosadiynoic acid is a highly specific, selective, high affinity and orally active acyl-CoA oxidase-1 (ACOX1) inhibitor. 10,12-Tricosadiynoic acid can treat high fat diet- or obesity-induced metabolic diseases by improving mitochondrial lipid and ROS metabolism. 10,12-Tricosadiynoic acid is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.
For research use only. We do not sell to patients.
- Purity: 99.95%
- CAS No.: 66990-30-5
- Formula: C23H38O2
- Molecular Weight:346.55
-
Storage:
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
Publications Citing Use of MedChemExpress (MCE) 10,12-Tricosadiynoic acid
More- Exp Mol Med. 2026 May;58(5):1642-1656. [Abstract]
- J Adv Res. 2025 Nov 20:S2090-1232(25)00927-0. [Abstract]
- Adv Sci (Weinh). 2025 Jul 29:e05436. [Abstract]
- Phytomedicine. 2024 Jan:123:155183. [Abstract]
- Environ Int. 2023 Aug:178:108138. [Abstract]
- Leukemia. 2025 Mar;39(3):720-733. [Abstract]
- Free Radic Biol Med. 2025 Aug 13:240:183-196. [Abstract]
- Brain Pathol. 2026 Mar 1:e70074. [Abstract]
- Dev Comp Immunol. 2022 Nov:136:104501. [Abstract]
- Res Sq. 2026 Mar 5.
Customer Validation & Images
-
Cell Proliferation/Viability Assay
-
In Vivo Efficacy Study
-
WB
-
RT-PCR
-
Bio/Physico-chemical Assay
Biological Activity
Acyl-CoA oxidase-1 (ACOX1)[1].
10,12-Tricosadiynoic acid-CoA rapidly inhibits ACOX1 activity in a time- and concentration-dependent manner. The activity of ACOX1 decreases by nearly 95% after 5 min of incubation with 10 eq of 10,12-Tricosadiynoic acid-CoA. ACOX1 activity is inhibited only if free 10,12-Tricosadiynoic Acid is activated as the CoA thioester, the substrate form. Inhibition of ACOX1 by 10,12-Tricosadiynoic acid-CoA is irreversible. And the kinetics parameters KI and kinact are calculated to be 680 nm and 3.18 min 1, respectively[1].
10,12-Tricosadiynoic acid is the precursor of 10,12-Tricosadiynoic acid-CoA and is transformed into 10,12-Tricosadiynoic acid-CoA by peroxisomal very long chain acyl-CoA synthetase (VLACS) after entering into cells, and it inhibits ACOX1 in vivo[1].
10,12-Tricosadiynoic acid (500 nM) inhibits acyl-CoA oxidase-1 (ACOX1) activity. 10,12-Tricosadiynoic acid treatment abrogates the protective effect by Sirt5 siRNA[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
10,12-Tricosadiynoic acid (0 mg/kg, 37.5 mg/kg, 75 mg/kg, and 150 mg/kg diet) treatment does not affect weight gain, but significantly decreases peroxisomal β-oxidation in the liver, and increased body fat accumulation in Nile tilapia. The fish with impaired peroxisomal β-oxidation exhibited higher contents of serum lipid and peroxidation products, and alanine aminotransferase activity, and significantly lowered hepatic activities of superoxide dismutase and catalase[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:Male Wistar rats (210-230 g) fed with high fat diet[1]
-
Dosage:100 μg/kg
-
Administration:Oral gavage; daily; for 8 weeks
-
Result:Reduced hydrogen peroxide accumulation in high fat diet-fed rats, which significantly decreased hepatic lipid and ROS contents, reduced body weight gain, and decreased serum triglyceride and insulin levels.
Chemical Information
-
CAS No. 66990-30-5
-
Appearance Solid
-
Molecular Weight 346.55
-
Formula C23H38O2
-
Color Off-white to light blue
-
SMILES
CCCCCCCCCCC#CC#CCCCCCCCCC(O)=O
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Publications (10)
-
Journal Impact Factor
-
Most Recent
-
Exp Mol Med
Cannabidiol triggers fatty acids β-oxidation mediated by Stat2 to facilitate intestinal stem cells regeneration post radiation. [Abstract]2026 May;58(5):1642-1656. PMID: 42120478 -
J Adv Res
Peroxisomal dysfunction in cardiac adipose tissue is involved in obesity-associated cardiac hypertrophy. [Abstract]2025 Nov 20:S2090-1232(25)00927-0. PMID: 41274637 -
Adv Sci (Weinh)
2025 Jul 29:e05436. PMID: 40726407
10,12-Tricosadiynoic acid purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Jul 29:e05436. [Abstract]
Effects of mild ACOX1 inhibition (30 µM, 10,12-Tricosadiynoic acid (ACOX1i) vs. DMSO, 72h) in the different pHe condi&ons with supplementa&on of 50 µM DHA (FA-free BSA as vehicle) (N=3, n=6).
10,12-Tricosadiynoic acid purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Jul 29:e05436. [Abstract]
10,12-Tricosadiynoic acid (ACOX1i, 10 mg/kg) significantly reduced tumor growth in mice fed the ω3 PUFA‐rich diet, but not in those on the control diet.
-
Phytomedicine
Shenge Formula attenuates high-fat diet-induced obesity and fatty liver via inhibiting ACOX1. [Abstract]2024 Jan:123:155183. PMID: 37992491
10,12-Tricosadiynoic acid purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Jan:123:155183. [Abstract]
10,12-Tricosadiynoic acid (TDYA, 24 h) significantly inhibited ACOX1 activity in AML12 cells.
-
Environ Int
PPARα/ACOX1 as a novel target for hepatic lipid metabolism disorders induced by per- and polyfluoroalkyl substances: An integrated approach. [Abstract]2023 Aug:178:108138. PMID: 37572494
10,12-Tricosadiynoic acid purchased from MedChemExpress. Usage Cited in: Environ Int. 2023 Aug:178:108138. [Abstract]
10,12-Tricosadiynoic acid (TDYA, 5 μM; 72 h) co-administered with 50 μM PFOA or PFOS for 72 h significantly attenuated PFOA- and PFOS-induced mitochondrial membrane potential collapse in AML12 cells, compared with PFOA or PFOS treatment alone.
-
Leukemia
2025 Mar;39(3):720-733. PMID: 39885295
10,12-Tricosadiynoic acid purchased from MedChemExpress. Usage Cited in: Leukemia. 2025 Mar;39(3):720-733. [Abstract]
10,12-Tricosadiynoic acid (TDYA, 50 μM; 36 h) inhibited the activating phosphorylation of MET (MET Y1349/1356) and IGF1R (IGF1R Y1165/1166) in ARH77 cells, but had no effect on other proteins.
-
Free Radic Biol Med
2025 Aug 13:240:183-196. PMID: 40816648
10,12-Tricosadiynoic acid purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2025 Aug 13:240:183-196. [Abstract]
RT-qPCR detection of ACOX1 mRNA expression in mouse gastrocnemius muscle tissue. The results showed that 10,12-Tricosadiynoic acid (TA, 100 μg/kg; i.g.; once daily for 10 days) significantly inhibited the expression of ACOX1 mRNA.
10,12-Tricosadiynoic acid purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2025 Aug 13:240:183-196. [Abstract]
Enzyme activity assay of ACOX1 in mouse gastrocnemius muscle tissues. NC: Normal Control group; Dex: Model group; FA: Model + FA group; TA: Model + TA group. The results showed that 10,12-Tricosadiynoic acid (TA, 100 μg/kg; i.g.; once daily for 10 days) significantly inhibited the enzyme activity of ACOX1.
-
Brain Pathol
2026 Mar 1:e70074. PMID: 41765417 -
Dev Comp Immunol
Role of acyl-coenzyme A oxidase 1 (ACOX1) on palmitate-induced inflammation and ROS production of macrophages in large yellow croaker (Larimichthys crocea). [Abstract]2022 Nov:136:104501. PMID: 35961593 -
Solvent & Solubility
DMSO : 16.67 mg/mL (48.10 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 5 mg/mL (14.43 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 5 mg/mL (14.43 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
-
Data Sheet (273 KB)
-
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
-
Handling Instructions (2659 KB)
References
[1]. Zeng J, et al. Specific Inhibition of Acyl-CoA Oxidase-1 by an Acetylenic Acid Improves Hepatic Lipid and Reactive Oxygen Species (ROS) Metabolism in Rats Fed a High Fat Diet. J Biol Chem. 2017 Mar 3;292(9):3800-3809. [Content Brief]
[2]. Takuto Chiba, et al. Sirtuin 5 Regulates Proximal Tubule Fatty Acid Oxidation to Protect against AKI. J Am Soc Nephrol. 2019 Dec;30(12):2384-2398. [Content Brief]
[3]. Yan Liu, et al. Impaired peroxisomal fat oxidation induces hepatic lipid accumulation and oxidative damage in Nile tilapia. Fish Physiol Biochem. 2020 Aug;46(4):1229-1242. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.8856 mL | 14.4279 mL | 28.8559 mL | 72.1397 mL |
| 5 mM | 0.5771 mL | 2.8856 mL | 5.7712 mL | 14.4279 mL | |
| 10 mM | 0.2886 mL | 1.4428 mL | 2.8856 mL | 7.2140 mL | |
| 15 mM | 0.1924 mL | 0.9619 mL | 1.9237 mL | 4.8093 mL | |
| 20 mM | 0.1443 mL | 0.7214 mL | 1.4428 mL | 3.6070 mL | |
| 25 mM | 0.1154 mL | 0.5771 mL | 1.1542 mL | 2.8856 mL | |
| 30 mM | 0.0962 mL | 0.4809 mL | 0.9619 mL | 2.4047 mL | |
| 40 mM | 0.0721 mL | 0.3607 mL | 0.7214 mL | 1.8035 mL |
10,12-Tricosadiynoic acid Related Classifications
HY-135425 Related Classifications
Powered by Bioz