2-Hydroxyisobutyric acid

Name: 2-Hydroxyisobutyric acid
Brand: MedChemExpress
SKU: HY-W015924
Rating: 4.5 (0 reviews)

Cat. No.: HY-W015924 Pureza: 98.0%: Ficha de datos
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2-Hydroxyisobutyric acid (2-HIBA) is a selective modulator of the Insulin/IGF-1 pathway and the p38 MAPK pathway, which reduces reactive oxygen species (ROS) and fat accumulation in Caenorhabditis elegans. 2-Hydroxyisobutyric acid promotes β-oxidation and inhibits fatty acid synthesis by upregulating SKN-1/NRF2 and downregulating SREBP-1c transcription factors. 2-Hydroxyisobutyric acid has anti-aging and lipid-lowering effects, and can be used to study metabolic diseases such as obesity and diabetes. 2-Hydroxyisobutyric acid is also a renewable precursor of methacrylate through 2-HIB-CoA mutase-mediated biosynthesis[1][2].

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2-Hydroxyisobutyric acid Estructura química

No. CAS : 594-61-6

Tamaño	Stock	Cantidad

Muestra gratis (0.1 - 0.2 mg)	Solicite ahora
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	En stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	En stock	Estimated Time of Arrival: December 31
Solid
100 mg	En stock	Estimated Time of Arrival: December 31
500 mg	En stock	Estimated Time of Arrival: December 31
1 g	En stock	Estimated Time of Arrival: December 31
5 g	Obtener un presupuesto
10 g	Obtener un presupuesto

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Based on 1 Customer Validation

Other Forms of 2-Hydroxyisobutyric acid:

2-Hydroxyisobutyric acid-d₆ In-stock
2-Hydroxyisobutyric acid (Standard) In-stock

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Descripciòn

In Vivo

2-Hydroxyisobutyric acid (10 mM, 20 mM; culture medium supplementation; continuous administration from embryonic hatching; 25 days) extends the lifespan and delays senescence of wild-type C. elegans N2 by reducing lipofuscin accumulation and ROS levels^[1].
2-Hydroxyisobutyric acid (10 mM; culture medium supplementation; 1 day) alters lipid metabolism in C. elegans by upregulating sbp-1, fat-7, and sams-1 transcription while downregulating acs-2 and ech-1^[1].
2-Hydroxyisobutyric acid (10 mM; medium addition; duration; 1 d) activates p38 MAPK signaling in the C. elegans transgenic line LD1 (skn-1::GFP) and inhibits the insulin/IGF-1 pathway in TJ356 (daf-16::GFP)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Peso molecular

104.11

Fòrmula

C₄H₈O₃

No. CAS

594-61-6

Appearance

Solid

Color

White to pink

SMILES

CC(C)(O)C(O)=O

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Endogenous metabolite

Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvente y solubilidad

In Vitro:

DMSO : 100 mg/mL (960.52 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : ≥ 100 mg/mL (960.52 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	9.6052 mL	48.0261 mL	96.0523 mL
5 mM	1.9210 mL	9.6052 mL	19.2104 mL
10 mM	0.9605 mL	4.8026 mL	9.6052 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Calculadora de molaridad
Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (24.01 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (24.01 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (24.01 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Pureza y Documentación

Purity: 98.0%

Select Batch:

Ficha de datos (273 KB) SDS (614 KB)

COA (245 KB) HNMR (234 KB)

Instrucciones de manejo (2659 KB)

Referencias

[1]. Schifano E, et al. 2-hydroxyisobutyric acid (2-HIBA) modulates ageing and fat deposition in Caenorhabditis elegans. Front Mol Biosci. 2022 Nov 23;9:986022. [Content Brief]

[2]. Przybylski D, et al. Exploiting mixtures of H2, CO2, and O2 for improved production of methacrylate precursor 2-hydroxyisobutyric acid by engineered Cupriavidus necator strains. Appl Microbiol Biotechnol. 2015 Mar;99(5):2131-45. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	9.6052 mL	48.0261 mL	96.0523 mL	240.1306 mL
	5 mM	1.9210 mL	9.6052 mL	19.2105 mL	48.0261 mL
	10 mM	0.9605 mL	4.8026 mL	9.6052 mL	24.0131 mL
	15 mM	0.6403 mL	3.2017 mL	6.4035 mL	16.0087 mL
	20 mM	0.4803 mL	2.4013 mL	4.8026 mL	12.0065 mL
	25 mM	0.3842 mL	1.9210 mL	3.8421 mL	9.6052 mL
	30 mM	0.3202 mL	1.6009 mL	3.2017 mL	8.0044 mL
	40 mM	0.2401 mL	1.2007 mL	2.4013 mL	6.0033 mL
	50 mM	0.1921 mL	0.9605 mL	1.9210 mL	4.8026 mL
	60 mM	0.1601 mL	0.8004 mL	1.6009 mL	4.0022 mL
	80 mM	0.1201 mL	0.6003 mL	1.2007 mL	3.0016 mL
	100 mM	0.0961 mL	0.4803 mL	0.9605 mL	2.4013 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.