2. Metabolic Enzyme/Protease Apoptosis NF-κB Immunology/Inflammation
  3. Endogenous Metabolite Ferroptosis Glutathione Peroxidase Aldehyde Dehydrogenase (ALDH) Apoptosis Reactive Oxygen Species (ROS) Keap1-Nrf2
  4. 3-Hydroxyphenylacetic acid

3-Hydroxyphenylacetic acid  (Synonyms: 3-ヒドロキシフェニル酢酸; m-ヒドロキシフェニル酢酸)

製品番号: HY-W001083 純度: 99.03%
COA 取扱説明書 Technical Support

3-Hydroxyphenylacetic acid is an orally active flavonoid metabolite produced by intestinal flora, with blood pressure-lowering activity. 3-Hydroxyphenylacetic acid can also inhibit ferroptosis by upregulating the expression of GPX4, thereby improving spermatogenic dysfunction in aged mice. In addition, abnormal levels of 3-Hydroxyphenylacetic acid are closely related to certain diseases, such as autism spectrum disorders.

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3-Hydroxyphenylacetic acid

3-Hydroxyphenylacetic acid 構造式

CAS 番号 : 621-37-4

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>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 44 在庫あり
Solution
10 mM * 1 mL in DMSO USD 44 在庫あり
Solid
100 mg $40 在庫あり
500 mg $70 在庫あり
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Based on 1 publication(s) in Google Scholar

Other Forms of 3-Hydroxyphenylacetic acid:

3-Hydroxyphenylacetic acid 関連抗体

Top Publications Citing Use of Products

Endogenous Metabolite アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示
Human Endogenous Metabolite Microbial Metabolite

Glutathione Peroxidase アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示
GPX1 GPX4

Aldehyde Dehydrogenase (ALDH) アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示
ALDH1 ALDH2 ALDH3

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製品説明

3-Hydroxyphenylacetic acid is an orally active flavonoid metabolite produced by intestinal flora, with blood pressure-lowering activity. 3-Hydroxyphenylacetic acid can also inhibit ferroptosis by upregulating the expression of GPX4, thereby improving spermatogenic dysfunction in aged mice. In addition, abnormal levels of 3-Hydroxyphenylacetic acid are closely related to certain diseases, such as autism spectrum disorders[1][2][3][4].

IC50 & Target

GPX4

 

体外実験

3-Hydroxyphenylacetic acid (0-50 μM; 6-9 h) can enhance total ALDH activity and completely inhibit acetaldehyde-induced cytotoxicity in mouse hepatocellular carcinoma Hepa1c1c7 cells[1].
3-Hydroxyphenylacetic acid (0.1-1 mM) can induce dose-dependent vasodilation in porcine coronary artery segments[2].
3-Hydroxyphenylacetic acid (50 μM; 48 h) can inhibit cell apoptosis, reduce ROS production, decrease MDA levels, increase GPx activity, and upregulate GPX4 protein expression in H2O2-induced senescent GC-2 cells[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

3-Hydroxyphenylacetic acid 関連抗体

Western Blot Analysis[3]

Cell Line: GC-2 cells treated H2O2
Concentration: 50 μM
Incubation Time: 48 h
Result: Significantly increased GPX4 protein expression, although no change was found for FTL, ACSL4, and xCT.
体内実験

3-Hydroxyphenylacetic acid (0.001-10 mg/kg; intravenous bolus; single dose) can reduce arterial blood pressure in spontaneously hypertensive rats[2].
3-Hydroxyphenylacetic acid (25 mg/kg; oral gavage; 6 weeks) can improve spermatogenic dysfunction in aged mice[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Spontaneously hypertensive rats (SHR) (average weight 356 ± 21 g)[2]
Dosage: 0.001, 0.01. 0.1, 0.5, 1, 2.5, 5 and 10 mg/kg (the volume applied was always 0.1 mL)
Administration: Intravenous bolus; single dose
Result: Decreased the systolic, diastolic, and mean arterial pressures of SHR in a dose-dependent manner, with 0.01 mg/kg significantly reducing diastolic pressure and 10 mg/kg showing the most significant antihypertensive effect.
Had no significant effect on heart rate.
Animal Model: Male C57BL/6J mice aged 18-20 months old[3]
Dosage: 25 mg/kg
Administration: Oral gavage; 6 weeks
Result: Increased sperm concentration.
Improved spermatogenesis with better-organized seminiferous tubules and increased numbers of spermatogonia, spermatocytes, spermatids, and spermatozoa.
Increased the expression of GPX4 and NRF2.
Increased total GPx activity, level of GSH, and ratio of GSH/GSSG, along with decreased MDA abundance, indicating restrained ferroptosis.
分子量

152.15

分子式

C8H8O3
CAS 番号
Appearance

Solid

Color

White to off-white

SMILES

OC1=CC=CC(CC(O)=O)=C1
Structure Classification
Initial Source
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
溶剤 & 溶解度
体外: 

DMSO : 50 mg/mL (328.62 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 25 mg/mL (164.31 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 6.5725 mL 32.8623 mL 65.7246 mL
5 mM 1.3145 mL 6.5725 mL 13.1449 mL
10 mM 0.6572 mL 3.2862 mL 6.5725 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

×
体積 （終了）

V2

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (16.43 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (16.43 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
純度とドキュメンテーション

純度: 99.77%

COA (248 KB) HNMR (118 KB) RP-HPLC (226 KB)

取扱説明書 (2659 KB)
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 6.5725 mL 32.8623 mL 65.7246 mL 164.3115 mL
5 mM 1.3145 mL 6.5725 mL 13.1449 mL 32.8623 mL
10 mM 0.6572 mL 3.2862 mL 6.5725 mL 16.4312 mL
15 mM 0.4382 mL 2.1908 mL 4.3816 mL 10.9541 mL
20 mM 0.3286 mL 1.6431 mL 3.2862 mL 8.2156 mL
25 mM 0.2629 mL 1.3145 mL 2.6290 mL 6.5725 mL
30 mM 0.2191 mL 1.0954 mL 2.1908 mL 5.4771 mL
40 mM 0.1643 mL 0.8216 mL 1.6431 mL 4.1078 mL
50 mM 0.1314 mL 0.6572 mL 1.3145 mL 3.2862 mL
60 mM 0.1095 mL 0.5477 mL 1.0954 mL 2.7385 mL
80 mM 0.0822 mL 0.4108 mL 0.8216 mL 2.0539 mL
100 mM 0.0657 mL 0.3286 mL 0.6572 mL 1.6431 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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3-Hydroxyphenylacetic acid Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

3-Hydroxyphenylacetic acid621-37-4Endogenous MetaboliteFerroptosisGlutathione PeroxidaseAldehyde Dehydrogenase (ALDH)ApoptosisReactive Oxygen Species (ROS)Keap1-Nrf2spontaneously hypertensive rats Hepa1c1c7 cellsGC-2 cellsInhibitorinhibitorinhibit

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