6-Thio-2'-Deoxyguanosine (Synonyms: 6-thio-dG; β-TGdR)

Name: 6-Thio-2'-Deoxyguanosine
Brand: MedChemExpress
SKU: HY-18762
Rating: 5.0 (2 reviews)

Cat. No.: HY-18762 Purity: 99.70%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

6-Thio-2'-Deoxyguanosine is a nucleoside analogue that can be incorporated into de novo-synthesized telomeres by telomerase.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

6-Thio-2'-Deoxyguanosine 화학구조

CAS No. : 789-61-7

사이즈	재고	수량
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	해외재고보유	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	해외재고보유	Estimated Time of Arrival: December 31
Solid
5 mg	해외재고보유	Estimated Time of Arrival: December 31
10 mg	해외재고보유	Estimated Time of Arrival: December 31
25 mg	해외재고보유	Estimated Time of Arrival: December 31
50 mg	해외재고보유	Estimated Time of Arrival: December 31
100 mg	해외재고보유	Estimated Time of Arrival: December 31
200 mg	견적 받기
500 mg	견적 받기

or 대량구매 문의

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 2 publication(s) in Google Scholar

2 Publications Citing Use of MCE 6-Thio-2'-Deoxyguanosine

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Biological Activity
Protocol
순도&문서
References
고객리뷰

제품 설명

6-Thio-2'-Deoxyguanosine is a nucleoside analogue that can be incorporated into de novo-synthesized telomeres by telomerase.

IC₅₀ & Target

DNA/RNA Synthesis^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
L1210	ED₅₀	>100 3 Compound: 1b	Inhibition of DNA synthesis in mouse L1210 cells assessed as inhibition of tritiated thymidine incorporation into nucleic acids Inhibition of DNA synthesis in mouse L1210 cells assessed as inhibition of tritiated thymidine incorporation into nucleic acids	[PMID: 845866]
L1210	ED₅₀	50 3 Compound: 1b	Inhibition of RNA synthesis in mouse L1210 cells assessed as inhibition of tritiated uridine incorporation into nucleic acids Inhibition of RNA synthesis in mouse L1210 cells assessed as inhibition of tritiated uridine incorporation into nucleic acids	[PMID: 845866]
L1210	ED₅₀	> 100 3 Compound: 1b	Inhibition of DNA synthesis in mouse L1210 cells assessed as inhibition of tritiated thymidine incorporation into nucleic acids Inhibition of DNA synthesis in mouse L1210 cells assessed as inhibition of tritiated thymidine incorporation into nucleic acids	[PMID: 845866]
L1210	ED₅₀	50 3 Compound: 1b	Inhibition of RNA synthesis in mouse L1210 cells assessed as inhibition of tritiated uridine incorporation into nucleic acids Inhibition of RNA synthesis in mouse L1210 cells assessed as inhibition of tritiated uridine incorporation into nucleic acids	[PMID: 845866]

In Vitro

Treatment with 6-Thio-2'-Deoxyguanosine results in rapid cell death for the vast majority of the cancer cell lines tested with IC₅₀s of between 0.7-2.9 μM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

In A549 lung cancer cell-based mouse xenograft studies, 6-Thio-2'-Deoxyguanosine causes a decrease of the tumor growth rate, superior to that observed with 6-thioguanine treatment. Additionally, 6-Thio-2'-Deoxyguanosine increases telomere dysfunction in tumor cells in vivo^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

분자량

283.31

화학식

C₁₀H₁₃N₅O₃S

CAS No.

789-61-7

Appearance

Solid

Color

White to yellow

SMILES

OC[C@@H]1[C@H](C[C@H](N2C=NC3=C2NC(N)=NC3=S)O1)O

선적

Room temperature in continental US; may vary elsewhere.

보관

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

용액&용해도

In Vitro:

DMSO : 50 mg/mL (176.49 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.5297 mL	17.6485 mL	35.2970 mL
5 mM	0.7059 mL	3.5297 mL	7.0594 mL
10 mM	0.3530 mL	1.7649 mL	3.5297 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

몰농도 계산기
농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 0.83 mg/mL (2.93 mM); Clear solution

This protocol yields a clear solution of ≥ 0.83 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (8.3 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 0.83 mg/mL (2.93 mM); Clear solution

This protocol yields a clear solution of ≥ 0.83 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (8.3 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 0.83 mg/mL (2.93 mM); Clear solution

This protocol yields a clear solution of ≥ 0.83 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (8.3 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

순도&문서

Purity: 99.70%

Select Batch:

Data Sheet (276 KB) SDS (394 KB)

COA (250 KB) HNMR (279 KB) LCMS (93 KB)

Handling Instructions (2659 KB)

References

[1]. Mender I, et al. Induction of telomere dysfunction mediated by the telomerase substrate precursor 6-thio-2'-deoxyguanosine. Cancer Discov. 2015 Jan;5(1):82-95. [Content Brief]

Cell Assay ^[1]	HCT116, A549, and H2882, HCC2429, HCC827, HCC15, H2087, HCC4017, HCC515, H2009, BJ and HCEC1 cells are plated in growth media in 96 well plates. Cells are incubated for 1 week and treated with varying concentrations of 6-Thio-2'-Deoxyguanosine and 6-thioguanine or DMSO every three days. The 96 well plates are analyzed for the CellTiterGlo luminescent cell viability assay^[1]. MCE 는 독립적으로 이러한 방법들의 정확성을 확인하지 않았습니다. 참고용으로만 봐주십시오.
Animal Administration	Athymic NCR nu/nu female mice (6 weeks old) are used. A549 cells are inoculated subcutaneously into the left and right dorsal flanks of the nude mice in 100 µL phosphate buffered saline (PBS). When tumors reach 40 mm³ average volume, mice are randomly divided into control, 6-thio-dG and 6-thioguanine treatment groups (3 animals in each group). Animals are injected intraperitoneally every two days for 17 days at a dose of 2mg/kg in 100 µL DMSO/PBS mixture per mouse. In addition, different animals are injected intratumorally every day for 16 days at a dose of Athymic NCR nu/nu female mice2.5mg/kg in 50 µL DMSO/PBS mixture per mouse. Tumor size is measured by calipers and recorded either every day or every two days^[1]. MCE 는 독립적으로 이러한 방법들의 정확성을 확인하지 않았습니다. 참고용으로만 봐주십시오.
References	[1]. Mender I, et al. Induction of telomere dysfunction mediated by the telomerase substrate precursor 6-thio-2'-deoxyguanosine. Cancer Discov. 2015 Jan;5(1):82-95. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.5297 mL	17.6485 mL	35.2970 mL	88.2426 mL
	5 mM	0.7059 mL	3.5297 mL	7.0594 mL	17.6485 mL
	10 mM	0.3530 mL	1.7649 mL	3.5297 mL	8.8243 mL
	15 mM	0.2353 mL	1.1766 mL	2.3531 mL	5.8828 mL
	20 mM	0.1765 mL	0.8824 mL	1.7649 mL	4.4121 mL
	25 mM	0.1412 mL	0.7059 mL	1.4119 mL	3.5297 mL
	30 mM	0.1177 mL	0.5883 mL	1.1766 mL	2.9414 mL
	40 mM	0.0882 mL	0.4412 mL	0.8824 mL	2.2061 mL
	50 mM	0.0706 mL	0.3530 mL	0.7059 mL	1.7649 mL
	60 mM	0.0588 mL	0.2941 mL	0.5883 mL	1.4707 mL
	80 mM	0.0441 mL	0.2206 mL	0.4412 mL	1.1030 mL
	100 mM	0.0353 mL	0.1765 mL	0.3530 mL	0.8824 mL

6-Thio-2'-Deoxyguanosine Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

6-Thio-2'-Deoxyguanosine789-61-76-thio-dG β-TGdRDNA/RNA SynthesisInhibitorinhibitorinhibit

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6-Thio-2'-Deoxyguanosine (Synonyms: 6-thio-dG; β-TGdR)

고객리뷰

6-Thio-2'-Deoxyguanosine Related Antibodies

2 Publications Citing Use of MCE 6-Thio-2'-Deoxyguanosine

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All DNA/RNA Synthesis Isoform Specific Products:

Biological Activity

Protocol

순도&문서

References

고객리뷰

6-Thio-2'-Deoxyguanosine Related Antibodies

몰농도 계산기

농도 희석 계산기

Complete Stock Solution Preparation Table

6-Thio-2'-Deoxyguanosine Related Classifications

Keywords:

최근 본 상품:

고객리뷰

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report