2. Cell Cycle/DNA Damage
  3. DNA/RNA Synthesis
  4. 6-Thio-2'-Deoxyguanosine

6-Thio-2'-Deoxyguanosine  (Synonyms: 6-thio-dG; β-TGdR)

製品番号: HY-18762 純度: 99.70%
COA 取扱説明書 Technical Support

6-Thio-2'-Deoxyguanosine is a nucleoside analogue that can be incorporated into de novo-synthesized telomeres by telomerase.

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6-Thio-2'-Deoxyguanosine

6-Thio-2'-Deoxyguanosine 構造式

CAS 番号 : 789-61-7

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 77 在庫あり
Solution
10 mM * 1 mL in DMSO USD 77 在庫あり
Solid
5 mg $70 在庫あり
10 mg $100 在庫あり
25 mg $220 在庫あり
50 mg $360 在庫あり
100 mg $540 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 2 publication(s) in Google Scholar

6-Thio-2'-Deoxyguanosine 関連抗体

Top Publications Citing Use of Products

DNA/RNA Synthesis アイソフォーム固有の製品をすべて表示:

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  • 純度とドキュメンテーション

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製品説明

6-Thio-2'-Deoxyguanosine is a nucleoside analogue that can be incorporated into de novo-synthesized telomeres by telomerase.

IC50 & Target

DNA/RNA Synthesis[1]
Cellular Effect
Cell Line Type Value Description References
L1210 ED50
>100 3
Compound: 1b
Inhibition of DNA synthesis in mouse L1210 cells assessed as inhibition of tritiated thymidine incorporation into nucleic acids
Inhibition of DNA synthesis in mouse L1210 cells assessed as inhibition of tritiated thymidine incorporation into nucleic acids
[PMID: 845866]
L1210 ED50
50 3
Compound: 1b
Inhibition of RNA synthesis in mouse L1210 cells assessed as inhibition of tritiated uridine incorporation into nucleic acids
Inhibition of RNA synthesis in mouse L1210 cells assessed as inhibition of tritiated uridine incorporation into nucleic acids
[PMID: 845866]
L1210 ED50
> 100 3
Compound: 1b
Inhibition of DNA synthesis in mouse L1210 cells assessed as inhibition of tritiated thymidine incorporation into nucleic acids
Inhibition of DNA synthesis in mouse L1210 cells assessed as inhibition of tritiated thymidine incorporation into nucleic acids
[PMID: 845866]
L1210 ED50
50 3
Compound: 1b
Inhibition of RNA synthesis in mouse L1210 cells assessed as inhibition of tritiated uridine incorporation into nucleic acids
Inhibition of RNA synthesis in mouse L1210 cells assessed as inhibition of tritiated uridine incorporation into nucleic acids
[PMID: 845866]
体外実験

Treatment with 6-Thio-2'-Deoxyguanosine results in rapid cell death for the vast majority of the cancer cell lines tested with IC50s of between 0.7-2.9 μM[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

6-Thio-2'-Deoxyguanosine 関連抗体
体内実験

In A549 lung cancer cell-based mouse xenograft studies, 6-Thio-2'-Deoxyguanosine causes a decrease of the tumor growth rate, superior to that observed with 6-thioguanine treatment. Additionally, 6-Thio-2'-Deoxyguanosine increases telomere dysfunction in tumor cells in vivo[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
臨床実験
分子量

283.31

分子式

C10H13N5O3S
CAS 番号
Appearance

Solid

Color

White to yellow

SMILES

OC[C@@H]1[C@H](C[C@H](N2C=NC3=C2NC(N)=NC3=S)O1)O
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
溶剤 & 溶解度
体外: 

DMSO : 50 mg/mL (176.49 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.5297 mL 17.6485 mL 35.2970 mL
5 mM 0.7059 mL 3.5297 mL 7.0594 mL
10 mM 0.3530 mL 1.7649 mL 3.5297 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

×
体積 （終了）

V2

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 0.83 mg/mL (2.93 mM); Clear solution

    This protocol yields a clear solution of ≥ 0.83 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (8.3 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 0.83 mg/mL (2.93 mM); Clear solution

    This protocol yields a clear solution of ≥ 0.83 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (8.3 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション

純度: 99.70%

COA (250 KB) HNMR (279 KB) LCMS (93 KB)

取扱説明書 (2659 KB)
参考文献
細胞実験
[1]

HCT116, A549, and H2882, HCC2429, HCC827, HCC15, H2087, HCC4017, HCC515, H2009, BJ and HCEC1 cells are plated in growth media in 96 well plates. Cells are incubated for 1 week and treated with varying concentrations of 6-Thio-2'-Deoxyguanosine and 6-thioguanine or DMSO every three days. The 96 well plates are analyzed for the CellTiterGlo luminescent cell viability assay[1].

MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
動物実験

Athymic NCR nu/nu female mice (6 weeks old) are used. A549 cells are inoculated subcutaneously into the left and right dorsal flanks of the nude mice in 100 µL phosphate buffered saline (PBS). When tumors reach 40 mm3 average volume, mice are randomly divided into control, 6-thio-dG and 6-thioguanine treatment groups (3 animals in each group). Animals are injected intraperitoneally every two days for 17 days at a dose of 2mg/kg in 100 µL DMSO/PBS mixture per mouse. In addition, different animals are injected intratumorally every day for 16 days at a dose of Athymic NCR nu/nu female mice2.5mg/kg in 50 µL DMSO/PBS mixture per mouse. Tumor size is measured by calipers and recorded either every day or every two days[1].

MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.5297 mL 17.6485 mL 35.2970 mL 88.2426 mL
5 mM 0.7059 mL 3.5297 mL 7.0594 mL 17.6485 mL
10 mM 0.3530 mL 1.7649 mL 3.5297 mL 8.8243 mL
15 mM 0.2353 mL 1.1766 mL 2.3531 mL 5.8828 mL
20 mM 0.1765 mL 0.8824 mL 1.7649 mL 4.4121 mL
25 mM 0.1412 mL 0.7059 mL 1.4119 mL 3.5297 mL
30 mM 0.1177 mL 0.5883 mL 1.1766 mL 2.9414 mL
40 mM 0.0882 mL 0.4412 mL 0.8824 mL 2.2061 mL
50 mM 0.0706 mL 0.3530 mL 0.7059 mL 1.7649 mL
60 mM 0.0588 mL 0.2941 mL 0.5883 mL 1.4707 mL
80 mM 0.0441 mL 0.2206 mL 0.4412 mL 1.1030 mL
100 mM 0.0353 mL 0.1765 mL 0.3530 mL 0.8824 mL
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6-Thio-2'-Deoxyguanosine Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

6-Thio-2'-Deoxyguanosine789-61-76-thio-dG β-TGdRDNA/RNA SynthesisInhibitorinhibitorinhibit

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製品名:
6-Thio-2'-Deoxyguanosine
製品番号:
HY-18762
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