1. Metabolic Enzyme/Protease
  2. Endogenous Metabolite

2'-Deoxyinosine 

Cat. No.: HY-W008638
Handling Instructions

2’-deoxyadenosine inhibits the growth of human colon-carcinoma cell lines and is found to be associated with purine nucleoside phosphorylase (PNP) deficiency.

For research use only. We do not sell to patients.

2'-Deoxyinosine Chemical Structure

2'-Deoxyinosine Chemical Structure

CAS No. : 890-38-0

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  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

2’-deoxyadenosine inhibits the growth of human colon-carcinoma cell lines and is found to be associated with purine nucleoside phosphorylase (PNP) deficiency.

IC50 & Target

Human Endogenous Metabolite

 

In Vitro

In the absence of deoxycoformycin, 2’-deoxyadenosine is primarily deaminated to 2’-deoxyinosine and then converted into hypoxanthine. In the presence of the inhibitor, the deoxynucleoside, in addition to a phosphorylation process, undergoes phosphorolytic cleavage giving rise to adenine. The conversion of 2’-deoxyadenosine to adenine might represent a protective device, emerging when the activity of adenosine deaminase is reduced or inhibited. There is much evidence to indicate that the enzyme catalyzing this processs may be distinct from methylthioadenosine phosphorylase and S-adenosyl homocysteine hydrolase, which are the enzymes reported to be responsible for the formation of adenine from 28-deoxyadenosine in mammals[1].

Solvent & Solubility
In Vitro: 

10 mM in DMSO

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.9646 mL 19.8232 mL 39.6464 mL
5 mM 0.7929 mL 3.9646 mL 7.9293 mL
10 mM 0.3965 mL 1.9823 mL 3.9646 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Cell Assay
[1]

Various amounts of cells, ranging from 1,000 to 900,000, suspended in 3 mL of standard medium, are plated in 35-mm dishes and incubated in the absence (control) and in the presence of both 1 μM dCF and 0.1 mM dAdo, as indicated in each experiment. dCf was added to the standard medium 30 min before 2’-deoxyadenosine (dAdo). Furthermore, an incubation is performed in which 0.1 mM 2’-deoxyadenosine alone is added to the standard medium. After 4 days of incubation, the standard medium is withdrawn, then 0.5 mL of 0.025% trypsin containing 0.02% EDTA is added and kept for few minutes at 37°C. The cells are collected, diluted in an appropriate volume of standard medium, and counted[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

252.23

Formula

C₁₀H₁₂N₄O₄

CAS No.

890-38-0

SMILES

OC1=C2N=CN([[email protected]@H]3O[[email protected]](CO)[[email protected]@H](O)C3)C2=NC=N1

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Shipping

Room temperature in continental US; may vary elsewhere

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Product Name:
2'-Deoxyinosine
Cat. No.:
HY-W008638
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2'-Deoxyinosine

Cat. No.: HY-W008638