1. Metabolic Enzyme/Protease
  2. Endogenous Metabolite
  3. 2'-Deoxyinosine

2'-Deoxyinosine 

Cat. No.: HY-W008638 Purity: ≥98.0%
Handling Instructions

2’-deoxyadenosine inhibits the growth of human colon-carcinoma cell lines and is found to be associated with purine nucleoside phosphorylase (PNP) deficiency.

For research use only. We do not sell to patients.

2'-Deoxyinosine Chemical Structure

2'-Deoxyinosine Chemical Structure

CAS No. : 890-38-0

Size Price Stock Quantity
Solution
10 mM * 1 mL USD 55 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 55 In-stock
Estimated Time of Arrival: December 31
Solid
100 mg USD 50 In-stock
Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

2’-deoxyadenosine inhibits the growth of human colon-carcinoma cell lines and is found to be associated with purine nucleoside phosphorylase (PNP) deficiency.

IC50 & Target

Human Endogenous Metabolite

 

In Vitro

In the absence of deoxycoformycin, 2’-deoxyadenosine is primarily deaminated to 2’-deoxyinosine and then converted into hypoxanthine. In the presence of the inhibitor, the deoxynucleoside, in addition to a phosphorylation process, undergoes phosphorolytic cleavage giving rise to adenine. The conversion of 2’-deoxyadenosine to adenine might represent a protective device, emerging when the activity of adenosine deaminase is reduced or inhibited. There is much evidence to indicate that the enzyme catalyzing this processs may be distinct from methylthioadenosine phosphorylase and S-adenosyl homocysteine hydrolase, which are the enzymes reported to be responsible for the formation of adenine from 28-deoxyadenosine in mammals[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

252.23

Formula

C₁₀H₁₂N₄O₄

CAS No.
Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (198.23 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.9646 mL 19.8232 mL 39.6464 mL
5 mM 0.7929 mL 3.9646 mL 7.9293 mL
10 mM 0.3965 mL 1.9823 mL 3.9646 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (9.91 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (9.91 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (9.91 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
Cell Assay
[1]

Various amounts of cells, ranging from 1,000 to 900,000, suspended in 3 mL of standard medium, are plated in 35-mm dishes and incubated in the absence (control) and in the presence of both 1 μM dCF and 0.1 mM dAdo, as indicated in each experiment. dCf was added to the standard medium 30 min before 2’-deoxyadenosine (dAdo). Furthermore, an incubation is performed in which 0.1 mM 2’-deoxyadenosine alone is added to the standard medium. After 4 days of incubation, the standard medium is withdrawn, then 0.5 mL of 0.025% trypsin containing 0.02% EDTA is added and kept for few minutes at 37°C. The cells are collected, diluted in an appropriate volume of standard medium, and counted[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

Purity: ≥98.0%

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Product Name:
2'-Deoxyinosine
Cat. No.:
HY-W008638
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