1. Anti-infection Apoptosis Cell Cycle/DNA Damage
  2. Influenza Virus Orthopoxvirus SARS-CoV Apoptosis Bcl-2 Family CDK
  3. Amantadine hydrochloride

Amantadine hydrochloride  (Synonyms: 1-Adamantanamine hydrochloride; 1-Adamantylamine hydrochloride; 1-Aminoadamantane hydrochloride)

Cat. No.: HY-B0402A Purity: ≥98.0%
COA Handling Instructions

Amantadine (1-Adamantanamine) hydrochloride is an orally avtive and potent antiviral agent with activity against influenza A viruses. Amantadine hydrochloride inhibits several ion channels such as NMDA and M2, and also inhibits Coronavirus ion channels. Amantadine hydrochloride also has anti-orthopoxvirus and anticancer activity. Amantadine hydrochloride can be used for Parkinson's disease, postoperative cognitive dysfunction (POCD) and COVID-19 research.

For research use only. We do not sell to patients.

Amantadine hydrochloride Chemical Structure

Amantadine hydrochloride Chemical Structure

CAS No. : 665-66-7

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Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
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Solution
10 mM * 1 mL in DMSO USD 39 In-stock
Solid
500 mg USD 35 In-stock
5 g USD 66 In-stock
10 g USD 92 In-stock
50 g USD 198 In-stock
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Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of Amantadine hydrochloride:

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Amantadine (1-Adamantanamine) hydrochloride is an orally avtive and potent antiviral agent with activity against influenza A viruses. Amantadine hydrochloride inhibits several ion channels such as NMDA and M2, and also inhibits Coronavirus ion channels. Amantadine hydrochloride also has anti-orthopoxvirus and anticancer activity. Amantadine hydrochloride can be used for Parkinson's disease, postoperative cognitive dysfunction (POCD) and COVID-19 research[1][2][3][4][5][6].

IC50 & Target

CDK2

 

Bcl-2

 

Bax

 

In Vitro

Amantadine hydrochloride (0-500 µM, 26 h) inhibits SARS-CoV-2 replication, with IC50 concentrations between 83 and 119 µM[4].
Amantadine hydrochloride (0-100 µg/mL, 24-72 h) markedly inhibits the proliferation of HepG2 and SMMC‑7721 cells[6].
Amantadine hydrochloride (0-75 µg/mL, 48 h) arrests the cell cycle at the G0/G1 phase and induces apoptosis[6].
Amantadine hydrochloride (0-75 µg/mL, 48 h) reduces the levels of the cell cycle‑related genes and proteins (cyclin D1, cyclin E and CDK2), reduces Bcl-2 and increases the Bax protein and mRNA levels[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[4]

Cell Line: Vero E6 cells
Concentration: 500 µM, 100 µM, 20 µM, 4 µM, and 8 nM
Incubation Time: 26 h
Result: Caused a concentration-dependent reduction (IC50=83 µM) of viral nucleic acids in the supernatant 26 h after infection at 10-500 µM. Caused a concentration-dependent reduction (IC50=119 µM) of viral nucleic acids in the cytosol 26 h after infection.

Cell Proliferation Assay[6]

Cell Line: Human HCC cell lines (HepG2 and SMMC-7721) and normal hepatocellular cells (L02 cells)
Concentration: 0, 1, 2, 5, 10, 25, 50 and 100 µg/mL
Incubation Time: 24, 48 and 72 h
Result: Inhibited cellular proliferation in a time- and dose-dependent manner in HepG2 and SMMC-7721 cells.

Cell Cycle Analysis[6]

Cell Line: HepG2 and SMMC-7721 cells
Concentration: 0, 10, 25, 50 and 75 µg/mL
Incubation Time: 48 h
Result: Significantly increased the population of HepG2 and SMMC-7721 cells in the G0/G1 phase in a dose-dependent manner, and significantly decreased the number of HepG2 cells in the S phase.

Apoptosis Analysis[6]

Cell Line: HepG2 and SMMC-7721 cells
Concentration: 0, 10, 25, 50 and 75 µg/mL
Incubation Time: 48 h
Result: Markedly increased the percentage of apoptotic HepG2 and SMMC-7721 cells (early- and late-stage apoptosis) in a dose-dependent manner.

Western Blot Analysis[6]

Cell Line: HepG2 and SMMC-7721 cells
Concentration: 0, 10, 25, 50 and 75 µg/mL
Incubation Time: 48 h
Result: Showed downregulation of cyclin D1, cyclin E and CDK2, and showed a decrease in Bcl-2 levels and an increase of Bax levels in HepG2 and SMMC-7721 cells.

RT-PCR[6]

Cell Line: HepG2 and SMMC-7721 cells
Concentration: 0, 10, 25, 50 and 75 µg/mL
Incubation Time: 48 h
Result: Revealed an increase in Bax and decrease in Bcl-2 genes.
In Vivo

Amantadine hydrochloride (25 mg/kg, IP, once daily for 3 days) inhibits surgery induced neuroinflammation and learning and memory impairment[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Fischer 344 rats (Four-month old, male, 290-330 g, 15 rats each group)[5]
Dosage: 25 mg/kg
Administration: IP, once daily for 3 days (the first dose at 15 min before surgery)
Result: Inhibited surgery induced neuroinflammation and learning and memory impairment, increased GDNF (glial cell line-derived neurotrophic factor) that was co-localized with glial fibrillary acidic protein (an astrocytic marker) in the hippocampus.
Clinical Trial
Molecular Weight

187.71

Formula

C10H18ClN

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

NC1(C2)C[C@@H]3C[C@@H](C[C@H]2C3)C1.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (532.74 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : ≥ 50 mg/mL (266.37 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.3274 mL 26.6368 mL 53.2737 mL
5 mM 1.0655 mL 5.3274 mL 10.6547 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 6.88 mg/mL (36.65 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 5.3274 mL 26.6368 mL 53.2737 mL 133.1842 mL
5 mM 1.0655 mL 5.3274 mL 10.6547 mL 26.6368 mL
10 mM 0.5327 mL 2.6637 mL 5.3274 mL 13.3184 mL
15 mM 0.3552 mL 1.7758 mL 3.5516 mL 8.8789 mL
20 mM 0.2664 mL 1.3318 mL 2.6637 mL 6.6592 mL
25 mM 0.2131 mL 1.0655 mL 2.1309 mL 5.3274 mL
30 mM 0.1776 mL 0.8879 mL 1.7758 mL 4.4395 mL
40 mM 0.1332 mL 0.6659 mL 1.3318 mL 3.3296 mL
50 mM 0.1065 mL 0.5327 mL 1.0655 mL 2.6637 mL
60 mM 0.0888 mL 0.4439 mL 0.8879 mL 2.2197 mL
80 mM 0.0666 mL 0.3330 mL 0.6659 mL 1.6648 mL
100 mM 0.0533 mL 0.2664 mL 0.5327 mL 1.3318 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Amantadine hydrochloride
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