Cat. No.: HY-B0402A

Amantadine hydrochloride

(Synonyms: 1-Adamantanamine hydrochloride; 1-Adamantylamine hydrochloride; 1-Aminoadamantane hydrochloride)

11 Cited Publications

Customer Review

Based on 11 publication(s) in Google Scholar

Amantadine (1-Adamantanamine) hydrochloride is an orally avtive and potent antiviral agent with activity against influenza A viruses. Amantadine hydrochloride inhibits several ion channels such as NMDA and M2, and also inhibits Coronavirus ion channels. Amantadine hydrochloride also has anti-orthopoxvirus and anticancer activity. Amantadine hydrochloride can be used for Parkinson's disease, postoperative cognitive dysfunction (POCD) and COVID-19 research.

For research use only. We do not sell to patients.

Purity: 98.0%
CAS No.: 665-66-7
Formula: C₁₀H₁₈ClN
Molecular Weight:187.71

Storage:

Store at room temperature, keep dry and cool.

In solvent -80°C, 1 year , -20°C, 6 months

Amantadine hydrochloride Chemical Structure

CAS No.: 665-66-7

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (11)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

CDK2

Bcl-2

Bax

All Bcl-2 Family Isoforms :

All CDK Isoforms :

In Vitro

Amantadine hydrochloride (0-500 μM, 26 h) inhibits SARS-CoV-2 replication, with IC₅₀ concentrations between 83 and 119 μM^[4].
? Amantadine hydrochloride (0-100 μg/mL, 24-72 h) markedly inhibits the proliferation of HepG2 and SMMC?7721 cells^[6].
? Amantadine hydrochloride (0-75 μg/mL, 48 h) arrests the cell cycle at the G0/G1 phase and induces apoptosis^[6].
? ? Amantadine hydrochloride (0-75 μg/mL, 48 h) reduces the levels of the cell cycle?related genes and proteins (cyclin D1, cyclin E and CDK2), reduces Bcl-2 and increases the Bax protein and mRNA levels^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[4]

Cell Line:

Vero E6 cells
Concentration:

500 µM, 100 µM, 20 µM, 4 µM, and 8 nM
Incubation Time:

26 h
Result:

Caused a concentration-dependent reduction (IC₅₀=83 µM) of viral nucleic acids in the supernatant 26 h after infection at 10-500 µM. Caused a concentration-dependent reduction (IC₅₀=119 µM) of viral nucleic acids in the cytosol 26 h after infection.

Cell Proliferation Assay^[6]

Cell Line:

Human HCC cell lines (HepG2 and SMMC-7721) and normal hepatocellular cells (L02 cells)
Concentration:

0, 1, 2, 5, 10, 25, 50 and 100 µg/mL
Incubation Time:

24, 48 and 72 h
Result:

Inhibited cellular proliferation in a time- and dose-dependent manner in HepG2 and SMMC-7721 cells.

Cell Cycle Analysis^[6]

Cell Line:

HepG2 and SMMC-7721 cells
Concentration:

0, 10, 25, 50 and 75 µg/mL
Incubation Time:

48 h
Result:

Significantly increased the population of HepG2 and SMMC-7721 cells in the G0/G1 phase in a dose-dependent manner, and significantly decreased the number of HepG2 cells in the S phase.

Apoptosis Analysis^[6]

Cell Line:

HepG2 and SMMC-7721 cells
Concentration:

0, 10, 25, 50 and 75 µg/mL
Incubation Time:

48 h
Result:

Markedly increased the percentage of apoptotic HepG2 and SMMC-7721 cells (early- and late-stage apoptosis) in a dose-dependent manner.

Western Blot Analysis^[6]

Cell Line:

HepG2 and SMMC-7721 cells
Concentration:

0, 10, 25, 50 and 75 µg/mL
Incubation Time:

48 h
Result:

Showed downregulation of cyclin D1, cyclin E and CDK2, and showed a decrease in Bcl-2 levels and an increase of Bax levels in HepG2 and SMMC-7721 cells.

RT-PCR^[6]

Cell Line:

HepG2 and SMMC-7721 cells
Concentration:

0, 10, 25, 50 and 75 µg/mL
Incubation Time:

48 h
Result:

Revealed an increase in Bax and decrease in Bcl-2 genes.

In Vivo

Amantadine hydrochloride (25 mg/kg, IP, once daily for 3 days) inhibits surgery induced neuroinflammation and learning and memory impairment^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Fischer 344 rats (Four-month old, male, 290-330 g, 15 rats each group)^[5]
Dosage:

25 mg/kg
Administration:

IP, once daily for 3 days (the first dose at 15 min before surgery)
Result:

Inhibited surgery induced neuroinflammation and learning and memory impairment, increased GDNF (glial cell line-derived neurotrophic factor) that was co-localized with glial fibrillary acidic protein (an astrocytic marker) in the hippocampus.

Chemical Information

CAS No. 665-66-7
Appearance Solid
Molecular Weight 187.71
Formula C₁₀H₁₈ClN
Color White to off-white
SMILES

NC1(C2)C[C@@H]3C[C@@H](C[C@H]2C3)C1.Cl
Synonyms

1-Adamantanamine hydrochloride; 1-Adamantylamine hydrochloride; 1-Aminoadamantane hydrochloride
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Store at room temperature, keep dry and cool

In solvent	-80°C	1 year
	-20°C	6 months

Publications (11)

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Journal Impact Factor

Signal Transduct Target Ther

Cathepsin L plays a key role in SARS-CoV-2 infection in humans and humanized mice and is a promising target for new drug development. [Abstract]

2021 Mar 27;6(1):134. PMID: 33774649
Int J Nanomedicine

The Optimization Design Of Lactoferrin Loaded HupA Nanoemulsion For Targeted Drug Transport Via Intranasal Route. [Abstract]

2019 Nov 27:14:9217-9234. PMID: 31819426
Emerg Microbes Infect

Efficient airborne transmission of influenza D virus in ferret models and serological evidence of human exposure in Northeast China. [Abstract]

2025 Dec;14(1):2564308. PMID: 41069204
Int J Mol Sci

Lycorine Inhibits Influenza Virus Replication by Affecting Nascent Nucleoporin Nup93 Synthesis. [Abstract]

2025 Jun 3;26(11):5358. PMID: 40508167
PLoS Pathog

Anti-orthopoxvirus drugs inhibit lumpy skin disease virus replication by targeting viral DNA polymerase. [Abstract]

2026 Jan 26;22(1):e1013903. PMID: 41587213
ACS Infect Dis

Development of a Universal Type A Influenza Viral RdRp-Induced Reporter System with Potential for Antiviral Drug Screening. [Abstract]

2025 Jun 13;11(6):1437-1447. PMID: 40421776
Virol J

Bisbenzylisoquinoline alkaloids inhibit influenza virus replication by disrupting endosomal acidification. [Abstract]

2025 Jun 4;22(1):181. PMID: 40468427
Open Forum Infect Dis

Toward Personalized Medicine: The Effect of Treatment of Chronic Enterovirus Diarrhea in an Immunocompromised Patient and the Correlation With In Vitro Models. [Abstract]

2025 Apr 10;12(5):ofaf212. PMID: 40302715
Chem Phys Lipids

Amantadine interactions with phase separated lipid membranes. [Abstract]

2024 Aug:262:105397. PMID: 38740276
Vet Microbiol

The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]

2026 May:316:110992. PMID: 41865607
bioRxiv

2026 Jun 10.

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (532.74 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : ≥ 50 mg/mL (266.37 mM)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

5.3274 mL

26.6368 mL

53.2737 mL

5 mM

1.0655 mL

5.3274 mL

10.6547 mL

10 mM

0.5327 mL

2.6637 mL

5.3274 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 6.88 mg/mL (36.65 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (285 KB)
SDS (779 KB)
- English - EN (779 KB)
- Français - FR (779 KB)
- Deutsch - DE (779 KB)
- Norwegian - NO (779 KB)
- Español - ES (779 KB)
- Swedish - SV (779 KB)
- Italian - IT (779 KB)
- Korean - KR (779 KB)
- Portuguese - PT (779 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Suzuki H, et al. Emergence of amantadine-resistant influenza A viruses: epidemiological study. J Infect Chemother. 2003;9(3):195-200. [Content Brief]

[2]. Hubsher G, et al. Amantadine: the journey from fighting flu to treating Parkinson disease. Neurology. 2012;78(14):1096-1099. [Content Brief]

[3]. Donald F Smee, et al. A review of compounds exhibiting anti-orthopoxvirus activity in animal models. Antiviral Res. 2003 Jan;57(1-2):41-52. [Content Brief]

[4]. Fink K, et al. Amantadine Inhibits SARS-CoV-2 In Vitro. Viruses. 2021 Mar 24;13(4):539. [Content Brief]

[5]. Zhang J, et al. Amantadine alleviates postoperative cognitive dysfunction possibly by increasing glial cell line-derived neurotrophic factor in rats. Anesthesiology. 2014 Oct;121(4):773-85. [Content Brief]

[6]. Lan Z, et al. Amantadine inhibits cellular proliferation and induces the apoptosis of hepatocellular cancer cells in vitro. Int J Mol Med. 2015;36(3):904-910. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / DMSO	1 mM	5.3274 mL	26.6368 mL	53.2737 mL	133.1842 mL
	5 mM	1.0655 mL	5.3274 mL	10.6547 mL	26.6368 mL
	10 mM	0.5327 mL	2.6637 mL	5.3274 mL	13.3184 mL
	15 mM	0.3552 mL	1.7758 mL	3.5516 mL	8.8789 mL
	20 mM	0.2664 mL	1.3318 mL	2.6637 mL	6.6592 mL
	25 mM	0.2131 mL	1.0655 mL	2.1309 mL	5.3274 mL
	30 mM	0.1776 mL	0.8879 mL	1.7758 mL	4.4395 mL
	40 mM	0.1332 mL	0.6659 mL	1.3318 mL	3.3296 mL
	50 mM	0.1065 mL	0.5327 mL	1.0655 mL	2.6637 mL
	60 mM	0.0888 mL	0.4439 mL	0.8879 mL	2.2197 mL
	80 mM	0.0666 mL	0.3330 mL	0.6659 mL	1.6648 mL
	100 mM	0.0533 mL	0.2664 mL	0.5327 mL	1.3318 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Amantadine hydrochloride

11 Cited Publications

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Amantadine hydrochloride

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Bioactive Compound Library

Anti-Infection Compound Library

Apoptosis Compound Library

Cell Cycle/DNA Damage Compound Library

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Rare Diseases Drug Library

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Publications Citing Use of MedChemExpress (MCE) Amantadine hydrochloride

All Bcl-2 Family Isoforms

All CDK Isoforms

Biological Activity

Chemical Information

Publications (11)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Amantadine hydrochloride Related Classifications

HY-B0402A Related Classifications