Amantadine (Synonyms: 1-Adamantanamine; 1-Aminoadamantane)

Name: Amantadine
Brand: MedChemExpress
SKU: HY-B0402
Rating: 4.5 (2 reviews)

Cat. No.: HY-B0402 Purity: 99.90%: FAQs
Data Sheet SDS COA Handling Instructions

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Amantadine (1-Adamantanamine) is an orally avtive and potent antiviral agent with activity against influenza A viruses. Amantadine inhibits several ion channels such as NMDA and M2, and also inhibits Coronavirus ion channels. Amantadine also has anti-orthopoxvirus and anticancer activity. Amantadine can be used for Parkinson's disease, postoperative cognitive dysfunction (POCD) and COVID-19 research.

For research use only. We do not sell to patients.

Amantadine Chemical Structure

CAS No. : 768-94-5

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Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of Amantadine:

2 Publications Citing Use of MCE Amantadine

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Biological Activity
Purity & Documentation
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Description

In Vitro

Amantadine (0-500 µM, 26 h) inhibits SARS-CoV-2 replication, with IC₅₀ concentrations between 83 and 119 µM^[4].
Amantadine (0-100 µg/mL, 24-72 h) markedly inhibits the proliferation of HepG2 and SMMC‑7721 cells^[6].
Amantadine (0-75 µg/mL, 48 h) arrests the cell cycle at the G0/G1 phase and induces apoptosis^[6].
Amantadine (0-75 µg/mL, 48 h) reduces the levels of the cell cycle‑related genes and proteins (cyclin D1, cyclin E and CDK2), reduces Bcl-2 and increases the Bax protein and mRNA levels^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[4]

Cell Line:	Vero E6 cells
Concentration:	500 µM, 100 µM, 20 µM, 4 µM, and 8 nM
Incubation Time:	26 h
Result:	Caused a concentration-dependent reduction (IC₅₀=83 µM) of viral nucleic acids in the supernatant 26 h after infection at 10-500 µM. Caused a concentration-dependent reduction (IC₅₀=119 µM) of viral nucleic acids in the cytosol 26 h after infection.

Cell Proliferation Assay^[6]

Cell Line:	Human HCC cell lines (HepG2 and SMMC-7721) and normal hepatocellular cells (L02 cells)
Concentration:	0, 1, 2, 5, 10, 25, 50 and 100 µg/mL
Incubation Time:	24, 48 and 72 h
Result:	Inhibited cellular proliferation in a time- and dose-dependent manner in HepG2 and SMMC-7721 cells.

Cell Cycle Analysis^[6]

Cell Line:	HepG2 and SMMC-7721 cells
Concentration:	0, 10, 25, 50 and 75 µg/mL
Incubation Time:	48 h
Result:	Significantly increased the population of HepG2 and SMMC-7721 cells in the G0/G1 phase in a dose-dependent manner, and significantly decreased the number of HepG2 cells in the S phase.

Apoptosis Analysis^[6]

Cell Line:	HepG2 and SMMC-7721 cells
Concentration:	0, 10, 25, 50 and 75 µg/mL
Incubation Time:	48 h
Result:	Markedly increased the percentage of apoptotic HepG2 and SMMC-7721 cells (early- and late-stage apoptosis) in a dose-dependent manner.

Western Blot Analysis^[6]

Cell Line:	HepG2 and SMMC-7721 cells
Concentration:	0, 10, 25, 50 and 75 µg/mL
Incubation Time:	48 h
Result:	Showed downregulation of cyclin D1, cyclin E and CDK2, and showed a decrease in Bcl-2 levels and an increase of Bax levels in HepG2 and SMMC-7721 cells.

RT-PCR^[6]

Cell Line:	HepG2 and SMMC-7721 cells
Concentration:	0, 10, 25, 50 and 75 µg/mL
Incubation Time:	48 h
Result:	Revealed an increase in Bax and decrease in Bcl-2 genes.

In Vivo

Amantadine (25 mg/kg, IP, once daily for 3 days) inhibits surgery induced neuroinflammation and learning and memory impairment^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Fischer 344 rats (Four-month old, male, 290-330 g, 15 rats each group)^[5]
Dosage:	25 mg/kg
Administration:	IP, once daily for 3 days (the first dose at 15 min before surgery)
Result:	Inhibited surgery induced neuroinflammation and learning and memory impairment, increased GDNF (glial cell line-derived neurotrophic factor) that was co-localized with glial fibrillary acidic protein (an astrocytic marker) in the hippocampus.

Clinical Trial

Molecular Weight

151.25

Formula

C₁₀H₁₇N

CAS No.

768-94-5

Appearance

Solid

Color

White to off-white

SMILES

NC12C[C@H]3C[C@@H](C2)C[C@@H](C1)C3

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 10 mg/mL (66.12 mM; ultrasonic and adjust pH to 2 with 1M HCl; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	6.6116 mL	33.0578 mL	66.1157 mL
5 mM	1.3223 mL	6.6116 mL	13.2231 mL
10 mM	0.6612 mL	3.3058 mL	6.6116 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1 mg/mL (6.61 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1 mg/mL (6.61 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 0.5 mg/mL (3.31 mM); Clear solution

This protocol yields a clear solution of ≥ 0.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.90%

Select Batch:

Data Sheet (253 KB) SDS (0 KB)

COA (248 KB) HNMR (228 KB) RP-HPLC (214 KB) MS (70 KB)

Handling Instructions (2659 KB)

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	6.6116 mL	33.0579 mL	66.1157 mL	165.2893 mL
	5 mM	1.3223 mL	6.6116 mL	13.2231 mL	33.0579 mL
	10 mM	0.6612 mL	3.3058 mL	6.6116 mL	16.5289 mL
	15 mM	0.4408 mL	2.2039 mL	4.4077 mL	11.0193 mL
	20 mM	0.3306 mL	1.6529 mL	3.3058 mL	8.2645 mL
	25 mM	0.2645 mL	1.3223 mL	2.6446 mL	6.6116 mL
	30 mM	0.2204 mL	1.1019 mL	2.2039 mL	5.5096 mL
	40 mM	0.1653 mL	0.8264 mL	1.6529 mL	4.1322 mL
	50 mM	0.1322 mL	0.6612 mL	1.3223 mL	3.3058 mL
	60 mM	0.1102 mL	0.5510 mL	1.1019 mL	2.7548 mL

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Amantadine768-94-51-Adamantanamine 1-AminoadamantaneInhibitorinhibitorinhibit

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Amantadine (Synonyms: 1-Adamantanamine; 1-Aminoadamantane)

Customer Review

Other Forms of Amantadine:

Amantadine Related Antibodies

2 Publications Citing Use of MCE Amantadine

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

Customer Review

Amantadine Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Keywords:

Your Recently Viewed Products:

Customer Review

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