1. Anti-infection
    Apoptosis
    Cell Cycle/DNA Damage
  2. Influenza Virus
    Orthopoxvirus
    SARS-CoV
    Apoptosis
    CDK
    Bcl-2 Family
  3. Amantadine sulfate

Amantadine sulfate (Synonyms: 1-Adamantanamine sulfate; 1-Aminoadamantane sulfate)

Cat. No.: HY-B0402B
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Amantadine (1-Adamantanamine) sulfate is an orally avtive and potent antiviral agent with activity against influenza A viruses. Amantadine sulfate inhibits several ion channels such as NMDA and M2, and also inhibits Coronavirus ion channels. Amantadine sulfate also has anti-orthopoxvirus and anticancer activity. Amantadine sulfate can be used for Parkinson's disease, postoperative cognitive dysfunction (POCD) and COVID-19 research.

For research use only. We do not sell to patients.

Amantadine sulfate Chemical Structure

Amantadine sulfate Chemical Structure

CAS No. : 31377-23-8

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Description

Amantadine (1-Adamantanamine) sulfate is an orally avtive and potent antiviral agent with activity against influenza A viruses. Amantadine sulfate inhibits several ion channels such as NMDA and M2, and also inhibits Coronavirus ion channels. Amantadine sulfate also has anti-orthopoxvirus and anticancer activity. Amantadine sulfate can be used for Parkinson's disease, postoperative cognitive dysfunction (POCD) and COVID-19 research[1][2][3][4][5][6].

IC50 & Target

CDK2

 

Bcl-2

 

Bax

 

In Vitro

Amantadine sulfate (0-500 µM, 26 h) inhibits SARS-CoV-2 replication, with IC50 concentrations between 83 and 119 µM[4].
Amantadine sulfate (0-100 µg/mL, 24-72 h) markedly inhibits the proliferation of HepG2 and SMMC‑7721 cells[6].
Amantadine sulfate (0-75 µg/mL, 48 h) arrests the cell cycle at the G0/G1 phase and induces apoptosis[6].
Amantadine sulfate (0-75 µg/mL, 48 h) reduces the levels of the cell cycle‑related genes and proteins (cyclin D1, cyclin E and CDK2), reduces Bcl-2 and increases the Bax protein and mRNA levels[6].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[4]

Cell Line: Vero E6 cells
Concentration: 500 µM, 100 µM, 20 µM, 4 µM, and 8 nM
Incubation Time: 26 h
Result: Caused a concentration-dependent reduction (IC50=83 µM) of viral nucleic acids in the supernatant 26 h after infection at 10-500 µM. Caused a concentration-dependent reduction (IC50=119 µM) of viral nucleic acids in the cytosol 26 h after infection.

Cell Proliferation Assay[6]

Cell Line: Human HCC cell lines (HepG2 and SMMC-7721) and normal hepatocellular cells (L02 cells)
Concentration: 0, 1, 2, 5, 10, 25, 50 and 100 µg/mL
Incubation Time: 24, 48 and 72 h
Result: Inhibited cellular proliferation in a time- and dose-dependent manner in HepG2 and SMMC-7721 cells.

Cell Cycle Analysis[6]

Cell Line: HepG2 and SMMC-7721 cells
Concentration: 0, 10, 25, 50 and 75 µg/mL
Incubation Time: 48 h
Result: Significantly increased the population of HepG2 and SMMC-7721 cells in the G0/G1 phase in a dose-dependent manner, and significantly decreased the number of HepG2 cells in the S phase.

Apoptosis Analysis[6]

Cell Line: HepG2 and SMMC-7721 cells
Concentration: 0, 10, 25, 50 and 75 µg/mL
Incubation Time: 48 h
Result: Markedly increased the percentage of apoptotic HepG2 and SMMC-7721 cells (early- and late-stage apoptosis) in a dose-dependent manner.

Western Blot Analysis[6]

Cell Line: HepG2 and SMMC-7721 cells
Concentration: 0, 10, 25, 50 and 75 µg/mL
Incubation Time: 48 h
Result: Showed downregulation of cyclin D1, cyclin E and CDK2, and showed a decrease in Bcl-2 levels and an increase of Bax levels in HepG2 and SMMC-7721 cells.

RT-PCR[6]

Cell Line: HepG2 and SMMC-7721 cells
Concentration: 0, 10, 25, 50 and 75 µg/mL
Incubation Time: 48 h
Result: Revealed an increase in Bax and decrease in Bcl-2 genes.
In Vivo

Amantadine sulfate (25 mg/kg, IP, once daily for 3 days) inhibits surgery induced neuroinflammation and learning and memory impairment[5].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Fischer 344 rats (Four-month old, male, 290-330 g, 15 rats each group)[5]
Dosage: 25 mg/kg
Administration: IP, once daily for 3 days (the first dose at 15 min before surgery)
Result: Inhibited surgery induced neuroinflammation and learning and memory impairment, increased GDNF (glial cell line-derived neurotrophic factor) that was co-localized with glial fibrillary acidic protein (an astrocytic marker) in the hippocampus.
Molecular Weight

200.18

Formula

C10H17N.1/2H2O4S

CAS No.
SMILES

NC1(C[[email protected]](C2)C3)C[[email protected]]3C[[email protected]]2C1.O=S(O)(O)=O.[1/2]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation
References
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Amantadine sulfate
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