2. GPCR/G Protein Metabolic Enzyme/Protease
  3. Bradykinin Receptor Endogenous Metabolite Ser/Thr Protease
  4. Bradykinin

ブラジキニン  (Synonyms: Bradykinin)

製品番号: HY-P0206 純度: 99.98%
COA 取扱説明書 Technical Support

Bradykinin is an effective endothelium-dependent vasodilator that can lower blood pressure. Bradykinin can induce contraction of bronchial and intestinal non-vascular smooth muscle, increase vascular permeability, and participate in the mechanism of pain.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

カスタムペプチド

CAS 番号 : 58-82-2

容量 価格（税別） 在庫状況 数量
1 mg $35 在庫あり
5 mg $79 在庫あり
10 mg $119 在庫あり
25 mg $198 在庫あり
50 mg $278 在庫あり
100 mg $388 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 17 publication(s) in Google Scholar

Other Forms of Bradykinin:

ブラジキニン 関連抗体

Top Publications Citing Use of Products

    Bradykinin purchased from MedChemExpress. Usage Cited in: iScience. 2024 May 22;27(6):110075.

    Representative Western blot images of Nrf2, xCT and GPX4 expression in HK-2 cells treated with 100 μM H2O2 and different concentrations of Bradykinin (0, 10, 50, 100, 500, 1000 nM) for 24 h.

    Bradykinin purchased from MedChemExpress. Usage Cited in: iScience. 2024 May 22;27(6):110075.

    Flow cytometry of reactive oxygen species (ROS) in HK-2 cells treated with 100 μM H2O2 alone or 100 μM H2O2 plus 100 nM Bradykinin.

    Bradykinin purchased from MedChemExpress. Usage Cited in: iScience. 2024 May 22;27(6):110075.

    Representative microscopy images of H2DCFDA-stained HK-2 cells treated with 100 μM H2O2 alone or 100 μM H2O2 plus 100 nM Bradykinin to detect ROS.

    Bradykinin purchased from MedChemExpress. Usage Cited in: iScience. 2022 Nov 8;25(11):105446.

    Fluo-4 direct staining shows the Bradykinin (100 nM)-induced global Ca2+ transients in the KO myoblast versus CTR.

    Bradykinin purchased from MedChemExpress. Usage Cited in: World J Surg Oncol. 2019 Feb 27;17(1):44.  [Abstract]

    H&E staining showed the flaps from rats treated with Bradykinin (150 μg/kg, i.p.) showed more neovascularization and less necrosis than those from the control group.

    Bradykinin Receptor アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Bradykinin B1 Receptor (B1R) Bradykinin B2 Receptor (B2R)

    Endogenous Metabolite アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Human Endogenous Metabolite Microbial Metabolite

    • 生物活性

    • プロトコル

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    Bradykinin is an effective endothelium-dependent vasodilator that can lower blood pressure. Bradykinin can induce contraction of bronchial and intestinal non-vascular smooth muscle, increase vascular permeability, and participate in the mechanism of pain[1][2][3][4][5].

    IC50 & Target

    Bradykinin B2 Receptor (B2R)

     

    Human Endogenous Metabolite

     

    体外実験

    Bradykinin is a potent vasodilator peptide that exerts its vasodilatory action through stimulation of specific endothelial B2 receptors, thereby causing the release of prostacyclin, NO, and EDHF[1]. Bradykinin has been reported to be involved in the progression of many types of cancer. Bradykinin treatment promotes the invasion and migration of colorectal cancer cells. Bradykinin treatment stimulates ERK1/2 activation and IL-6 production[2]. Exogenous bradykinin markedly inhibits TF expression in mRNA and protein level induced by LPS in a dose-dependent manner. The NO synthase antagonist L-NAME and PI3K inhibitor LY294002 dramatically abolish the inhibitory effects of bradykinin on tissue factor expression[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    ブラジキニン 関連抗体
    体内実験

    Application of 1 μM bradykinin to the ovary produces significant decreases in heart rate and mean arterial pressure. In vagotomized animals, application of 1 μM bradykinin to the ovary produces bradycardia and hypotension similar to the responses evoked when vagal innervation is intact[4].
    Vascular bradykinin can improve pancreatic microcirculation and hemorheology in rats with severe acute pancreatitis. The pancreatic microcirculatory blood flow volume and velocity in the vascular bradykinin treatment group increase gradually after 48 h[5].
    PI3K/Akt signaling pathway activation induced by bradykinin administration reduces the activity of GSK-3β and MAPK, and reduces NF-x03BA; B level in the nucleus, thereby inhibiting TF expression. Consistent with this, intraperitoneal injection of C57/BL6 mice with bradykinin also inhibits the thrombus formation induced by ligation of inferior vena cava[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    臨床実験
    分子量

    1060.21

    分子式

    C50H73N15O11
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    Sequence

    Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg
    Sequence Shortening

    RPPGFSPFR
    Structure Classification
    Initial Source
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Sealed storage, away from moisture
    Powder -80°C 2 years
      -20°C 1 year
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    H2O : ≥ 100 mg/mL (94.32 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 0.9432 mL 4.7160 mL 9.4321 mL
    5 mM 0.1886 mL 0.9432 mL 1.8864 mL
    10 mM 0.0943 mL 0.4716 mL 0.9432 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

    ×
    体積 （終了）

    V2

    体内:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 100 mg/mL (94.32 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    純度とドキュメンテーション

    純度: 99.98%

    COA (263 KB) RP-HPLC (263 KB) MS (71 KB) Elemental Analysis Report (122 KB)

    取扱説明書 (2659 KB)
    参考文献
    細胞実験
    [2]

    SW480 cells are pretreated with different concentrations of bradykinin (0, 0.1, 0.5, 1 μM), and then subjected to invasion and migration assays[2].

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
    動物実験
    [3][4]

    Rats: Bradykinin acetate is applied topically to the surface of the ovary with a thin piece of cotton (7×7 mm square) soaked with the solution. After application for 30 s, the cotton is removed. Each of the stimuli is delivered to the animal after observing stabilization of heart rate and mean arterial pressure[4].

    Mouse: Mice are randomly divided into 3 groups: Sham, Model and Bradykinin. Before surgical procedure, the mice of bradykinin group are intraperitoneally injected with bradykinin (10 mg/kg/d) once a day for three days. After ligation, the mice receive bradykinin injection for another two days and analgesic therapy is performed using buprenorphine at 0.1 mg/kg body weight for 3 days. The mice in other groups receive equal saline as control[3].

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 0.9432 mL 4.7160 mL 9.4321 mL 23.5802 mL
    5 mM 0.1886 mL 0.9432 mL 1.8864 mL 4.7160 mL
    10 mM 0.0943 mL 0.4716 mL 0.9432 mL 2.3580 mL
    15 mM 0.0629 mL 0.3144 mL 0.6288 mL 1.5720 mL
    20 mM 0.0472 mL 0.2358 mL 0.4716 mL 1.1790 mL
    25 mM 0.0377 mL 0.1886 mL 0.3773 mL 0.9432 mL
    30 mM 0.0314 mL 0.1572 mL 0.3144 mL 0.7860 mL
    40 mM 0.0236 mL 0.1179 mL 0.2358 mL 0.5895 mL
    50 mM 0.0189 mL 0.0943 mL 0.1886 mL 0.4716 mL
    60 mM 0.0157 mL 0.0786 mL 0.1572 mL 0.3930 mL
    80 mM 0.0118 mL 0.0590 mL 0.1179 mL 0.2948 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    Bradykinin Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Bradykinin58-82-2Bradykinin ReceptorEndogenous MetaboliteSer/Thr ProteaseSerine proteasesSerine endopeptidasesThreonine proteasesInhibitorinhibitorinhibit

    最近チェックした製品:

    オンラインお問い合わせ

    Your information is safe with us. * Required Fields.

    製品名

    		  

    カスタマ需要量 *

    お名前 *

    		 

    タイトル

    メールアドレス *

    		 

    電話番号 *

    デパートメント

    		 

    組纖名 *

    市区町村

    都道府県

    国或いは地域 *

    		      

    必ず会社名を記載ください。個人への返信は行いません。

    備考

    バルクお問い合わせ

    Inquiry Information

    製品名:
    Bradykinin
    製品番号:
    HY-P0206
    数量:
    MCE 日本正規代理店:

    カスタマーレビュー

    Thank You for Your Feedback!

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

    製品名

    		  

    Lot #

    お名前 *

    		 

    メールアドレス *

    電話番号 *

    		 

    部門 *

    組纖名 *

    		 

    国或いは地域 *

    備考

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.