2. GPCR/G Protein Metabolic Enzyme/Protease
  3. G protein-coupled Bile Acid Receptor 1 Endogenous Metabolite
  4. Deoxycholic acid

Deoxycholic acid  (Synonyms: Cholanoic Acid; Desoxycholic acid)

製品番号: HY-N0593 純度: 99.75%
COA 取扱説明書

Molarity Calculator

Deoxycholic acid (cholanoic acid), a bile acid, is a by-product of intestinal metabolism, that activates the G protein-coupled bile acid receptorTGR5.

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Deoxycholic acid 構造式

Deoxycholic acid 構造式

CAS 番号 : 83-44-3

容量 価格（税別） 在庫状況 数量
無料サンプル (0.1 - 0.5 mg)   今すぐ申し込む  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 73 在庫あり
Solution
10 mM * 1 mL in DMSO USD 73 在庫あり
Solid
100 mg USD 66 在庫あり
500 mg USD 92 在庫あり
1 g   お問い合わせ  
5 g   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 14 publication(s) in Google Scholar

Other Forms of Deoxycholic acid:

Deoxycholic acid 関連抗体

Top Publications Citing Use of Products

    Deoxycholic acid purchased from MedChemExpress. Usage Cited in: Cancer Cell Int. 2019 Jan 31;19:24.  [Abstract]

    The expression of CDX2, KLF4, HNF4α, and cadherin 17 is increased upon stimulation with DCA in a dose-dependent manner.

    Deoxycholic acid purchased from MedChemExpress. Usage Cited in: Cancer Cell Int. 2019 Jan 31;19:24.  [Abstract]

    The expression of CDX2, KLF4, HNF4α, and cadherin 17 is increased upon stimulation with DCA in a dose-dependent manner.

    Deoxycholic acid purchased from MedChemExpress. Usage Cited in: Cancer Cell Int. 2019 Jan 31;19:24.  [Abstract]

    Western blotting results indicated that the inhibition of SOX2 in bile acid-treated AGS cells is partially rescued in miR-21 knockdown AGS cells.

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    製品説明

    Deoxycholic acid (cholanoic acid), a bile acid, is a by-product of intestinal metabolism, that activates the G protein-coupled bile acid receptorTGR5[1][2].

    IC50 & Target

    Microbial Metabolite

     

    Human Endogenous Metabolite

     

    体外実験

    Deoxycholic acid (DCA) (100 μM) induces the production of gastric cancer cell line MGC803 resistant to acidified bile acids and enhances their survival and proliferation activity under bile acid stress[2].
    Deoxycholic acid (DCA) (100 μM)-induced resistant cells shows altered morphology, significantly elevated telomerase activity, better cell viability and reduces apoptosis compared to normal MGC803 cells[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Deoxycholic acid 関連抗体
    臨床実験
    分子量

    392.57

    分子式

    C24H40O4
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    C[C@@]1([C@@]2([H])[C@H](C)CCC(O)=O)[C@](CC2)([H])[C@@](CC[C@@]3([H])[C@@]4(CC[C@@H](O)C3)C)([H])[C@]4([H])C[C@@H]1O
    Structure Classification
    Initial Source
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : ≥ 100 mg/mL (254.73 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : < 0.1 mg/mL (insoluble)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.5473 mL 12.7366 mL 25.4732 mL
    5 mM 0.5095 mL 2.5473 mL 5.0946 mL
    10 mM 0.2547 mL 1.2737 mL 2.5473 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: 2.5 mg/mL (6.37 mM); Suspended solution; Need ultrasonic

      This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (6.37 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 99.82%

    COA (247 KB) RP-HPLC (256 KB)

    取扱説明書 (2659 KB)
    参考文献
    細胞実験
    [2]

    MGC803 cells are cultured in Roswell Park Memorial Institute media supplemented with 10% fetal calf serum and 100 U/mL Penicillin and 100 mg/mL Streptomycin. To generate MGC803-resistant cells, the pH value of the MGC803 culture medium is adjusted to the experimental conditions using the hydrochloric acid (A). The bile acids GCDA and Deoxycholic acid are diluted to optimal working concentrations of 100 μM (B) with culture medium, and the overall pH (A+B) is adjusted to pH 5.5, simulating the gastric environment. Initially, MGC803 cells are chronically exposed to acidified medium with bile acids (A+B) for 10 min every 24 h. The experimental time and conditions are optimized in our preliminary experiments, which show that 10 min is enough and does not result in cell damage. This procedure is repeated and it takes 60 weeks for the MGC803 cells to survive and proliferate under the exposure of A+B for 120 min. Control untreated cells are cultured in neutral RPMI medium at pH 7.4 in parallel to the resistant cells for 60 weeks. The morphological changes in MGC803 cells exposed to acidified bile acids (A+B) are documented at 30 and 60 weeks[2].

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.5473 mL 12.7366 mL 25.4732 mL 63.6829 mL
    5 mM 0.5095 mL 2.5473 mL 5.0946 mL 12.7366 mL
    10 mM 0.2547 mL 1.2737 mL 2.5473 mL 6.3683 mL
    15 mM 0.1698 mL 0.8491 mL 1.6982 mL 4.2455 mL
    20 mM 0.1274 mL 0.6368 mL 1.2737 mL 3.1841 mL
    25 mM 0.1019 mL 0.5095 mL 1.0189 mL 2.5473 mL
    30 mM 0.0849 mL 0.4246 mL 0.8491 mL 2.1228 mL
    40 mM 0.0637 mL 0.3184 mL 0.6368 mL 1.5921 mL
    50 mM 0.0509 mL 0.2547 mL 0.5095 mL 1.2737 mL
    60 mM 0.0425 mL 0.2123 mL 0.4246 mL 1.0614 mL
    80 mM 0.0318 mL 0.1592 mL 0.3184 mL 0.7960 mL
    100 mM 0.0255 mL 0.1274 mL 0.2547 mL 0.6368 mL
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    Deoxycholic acid Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass   Concentration   Volume   Molecular Weight *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Deoxycholic acid83-44-3Cholanoic Acid Desoxycholic acidG protein-coupled Bile Acid Receptor 1Endogenous MetaboliteG-protein coupled receptor 19GPCR19TGR5GPBAR1bile acidG protein-coupled bile acid receptorInhibitorinhibitorinhibit

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    製品名:
    Deoxycholic acid
    製品番号:
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