2. Membrane Transporter/Ion Channel
  3. Sodium Channel
  4. Dibucaine

Dibucaine  (Synonyms: Cinchocaine)

製品番号: HY-B0552 純度: 99.81%
COA 取扱説明書 Technical Support

Dibucaine (Cinchocaine) is a sodium channel inhibitor. Dibucaine is a potent SChE inhibitor.

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Dibucaine

Dibucaine 構造式

CAS 番号 : 85-79-0

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 60 在庫あり
Solution
10 mM * 1 mL in DMSO USD 60 在庫あり
Solid
500 mg $55 在庫あり
5 g $96 在庫あり
10 g $173 在庫あり
50 g   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

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Based on 4 publication(s) in Google Scholar

Other Forms of Dibucaine:

Dibucaine 関連抗体

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  • 生物活性

  • 純度とドキュメンテーション

  • 参考文献

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製品説明

Dibucaine (Cinchocaine) is a sodium channel inhibitor. Dibucaine is a potent SChE inhibitor[1][2].

Cellular Effect
Cell Line Type Value Description References
A 172 CC50
> 30 μM
Compound: 1
Cytotoxicity against human A172 cells after 3 days by neutral red dye-based photometric method
Cytotoxicity against human A172 cells after 3 days by neutral red dye-based photometric method
[PMID: 30912944]
A549 CC50
37.5 μM
Compound: 1
Cytotoxicity against human A549 cells after 3 days by neutral red dye-based photometric method
Cytotoxicity against human A549 cells after 3 days by neutral red dye-based photometric method
[PMID: 30912944]
HeLa CC50
36 μM
Compound: 1
Cytotoxicity against human HeLa cells after 3 days by neutral red dye-based photometric method
Cytotoxicity against human HeLa cells after 3 days by neutral red dye-based photometric method
[PMID: 30912944]
Hepatocyte IC50
470 μM
Compound: 18 Dibucaine
Inhibition of apamin-sensitive SKCa channel of guinea-pig hepatocytes
Inhibition of apamin-sensitive SKCa channel of guinea-pig hepatocytes
[PMID: 15225721]
RD CC50
56.6 μM
Compound: 1
Cytotoxicity against human RD cells after 3 days by neutral red dye-based photometric method
Cytotoxicity against human RD cells after 3 days by neutral red dye-based photometric method
[PMID: 30912944]
RD CC50
> 97.049 μM
Compound: 6d
Cytotoxicity against human RD cells infected with Enterovirus A71 assessed as reduction in cell viability measured by CCK-8 assay
Cytotoxicity against human RD cells infected with Enterovirus A71 assessed as reduction in cell viability measured by CCK-8 assay
[PMID: 32619885]
SH-SY5Y CC50
> 30 μM
Compound: 1
Cytotoxicity against human SH-SY5Y cells after 3 days by neutral red dye-based photometric method
Cytotoxicity against human SH-SY5Y cells after 3 days by neutral red dye-based photometric method
[PMID: 30912944]
体外実験

Dibucaine (Cinchocaine) reduces the degradation of BSA-gold complex in the reservosomes, which was not caused either by an inhibition of the whole proteolytic activity of the parasite or by a reduction on the expression levels of cruzipain[1]. Dibucaine, a quaternary ammonium compound, inhibited SChE to a minimum within 2 min in a reversible manner. The inhibition was very potent. It had an IC(50) of 5.3 microM with BuTch or 3.8 microM with AcTch. The inhibition was competitive with respect to BuTch with a K(i) of 1.3 microM and a linear-mixed type (competitive/noncompetitive) with respect to AcTch with inhibition constants, K(i) and K(I) of 0.66 and 2.5 microM, respectively. Dibucaine possesses a butoxy side chain that is similar to the butryl group of BuTch and longer by an ethylene group from AcTch[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Dibucaine 関連抗体
分子量

343.46

分子式

C20H29N3O2
CAS 番号
Appearance

Solid

Color

White to yellow

SMILES

O=C(C1=CC(OCCCC)=NC2=CC=CC=C12)NCCN(CC)CC
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
溶剤 & 溶解度
体外: 

DMSO : 100 mg/mL (291.15 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.9115 mL 14.5577 mL 29.1155 mL
5 mM 0.5823 mL 2.9115 mL 5.8231 mL
10 mM 0.2912 mL 1.4558 mL 2.9115 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

×
体積 （終了）

V2

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (7.28 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (7.28 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション

純度: 99.81%

COA (243 KB) LCMS (94 KB)

取扱説明書 (2659 KB)
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.9115 mL 14.5577 mL 29.1155 mL 72.7887 mL
5 mM 0.5823 mL 2.9115 mL 5.8231 mL 14.5577 mL
10 mM 0.2912 mL 1.4558 mL 2.9115 mL 7.2789 mL
15 mM 0.1941 mL 0.9705 mL 1.9410 mL 4.8526 mL
20 mM 0.1456 mL 0.7279 mL 1.4558 mL 3.6394 mL
25 mM 0.1165 mL 0.5823 mL 1.1646 mL 2.9115 mL
30 mM 0.0971 mL 0.4853 mL 0.9705 mL 2.4263 mL
40 mM 0.0728 mL 0.3639 mL 0.7279 mL 1.8197 mL
50 mM 0.0582 mL 0.2912 mL 0.5823 mL 1.4558 mL
60 mM 0.0485 mL 0.2426 mL 0.4853 mL 1.2131 mL
80 mM 0.0364 mL 0.1820 mL 0.3639 mL 0.9099 mL
100 mM 0.0291 mL 0.1456 mL 0.2912 mL 0.7279 mL
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Dibucaine Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Dibucaine85-79-0CinchocaineSodium ChannelNa channelsNa+ channelsSChEBSA-gold,Inhibitorinhibitorinhibit

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