1. Cell Cycle/DNA Damage
  2. DNA/RNA Synthesis
  3. Dithranol

Dithranol  (Synonyms: ジスラノール; Anthralin)

製品番号: HY-B0738 純度: 95.26%
COA 取扱説明書 Technical Support

Dithranol (Anthralin) is an anthraquinone derivative, with potent anti-psoriatic effects. Dithranol can inhibit DNA replication and repair.

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Dithranol

Dithranol 構造式

CAS 番号 : 1143-38-0

容量 価格(税別) 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
USD 46 在庫あり
Solution
10 mM * 1 mL in DMSO USD 46 在庫あり
Solid
50 mg $42 在庫あり
100 mg $66 在庫あり
250 mg $115 在庫あり
500 mg $144 在庫あり
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カスタマーレビュー

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製品説明

Dithranol (Anthralin) is an anthraquinone derivative, with potent anti-psoriatic effects. Dithranol can inhibit DNA replication and repair[1][2].

Cellular Effect
Cell Line Type Value Description References
HaCaT IC50
0.7 3
Compound: Anthralin
Antihyperproliferative activity against human HaCaT cells after 48 hrs by MTT assay
Antihyperproliferative activity against human HaCaT cells after 48 hrs by MTT assay
[PMID: 28410493]
HaCaT GI50
0.7 3
Compound: 14
Growth inhibition of human HaCaT cells after 72 hrs by neutral red uptake assay
Growth inhibition of human HaCaT cells after 72 hrs by neutral red uptake assay
[PMID: 22944332]
HaCaT GI50
0.7 3
Compound: 14
Growth inhibition of human HaCaT cells after 72 hrs by neutral red uptake assay
Growth inhibition of human HaCaT cells after 72 hrs by neutral red uptake assay
[PMID: 22944332]
HaCaT IC50
0.7 3
Compound: Anthralin
Antihyperproliferative activity against human HaCaT cells after 48 hrs by MTT assay
Antihyperproliferative activity against human HaCaT cells after 48 hrs by MTT assay
[PMID: 28410493]
HaCaT IC50
0.7 3
Compound: 2, Anthralin
Antiproliferative activity against human HaCaT cells assessed as dispersed cells after 48 hrs by phase-contrast microscopic analysis
Antiproliferative activity against human HaCaT cells assessed as dispersed cells after 48 hrs by phase-contrast microscopic analysis
[PMID: 20850910]
HaCaT IC50
0.7 3
Compound: 2
Antihyperproliferative activity against human HaCaT cells after 48 hrs by phase contrast microscopic analysis
Antihyperproliferative activity against human HaCaT cells after 48 hrs by phase contrast microscopic analysis
[PMID: 26840368]
HaCaT IC50
0.7 3
Compound: 1
Antiproliferative activity against human HaCaT cells after 48 hrs by phase contrast microscopy
Antiproliferative activity against human HaCaT cells after 48 hrs by phase contrast microscopy
[PMID: 20828887]
HaCaT IC50
0.7 3
Compound: 3
Antihyperproliferative activity against human HaCaT cells after 48 hrs by phase contrast microscopy
Antihyperproliferative activity against human HaCaT cells after 48 hrs by phase contrast microscopy
[PMID: 22845014]
HEK293 GI50
0.5 3
Compound: 14
Growth inhibition of human NHEK cells after 72 hrs by neutral red uptake assay
Growth inhibition of human NHEK cells after 72 hrs by neutral red uptake assay
[PMID: 22944332]
HaCaT IC50
0.7 3
Compound: 1
Antiproliferative activity against human HaCaT cells after 48 hrs by phase contrast microscopy
Antiproliferative activity against human HaCaT cells after 48 hrs by phase contrast microscopy
[PMID: 20452101]
HaCaT IC50
0.7 3
Compound: 5
Antihyperproliferative activity against human HaCaT cells assessed as inhibition of cell growth after 48 hrs by phase contrast microscopy
Antihyperproliferative activity against human HaCaT cells assessed as inhibition of cell growth after 48 hrs by phase contrast microscopy
[PMID: 24964246]
HaCaT IC50
0.6 3
Compound: 1/ Anthralin
Antiproliferative (inhibition of cell growth) activity against HaCaT cells (human keratinocyte line)
Antiproliferative (inhibition of cell growth) activity against HaCaT cells (human keratinocyte line)
[PMID: 9371243]
HaCaT IC50
0.7 3
Compound: 2
Antihyperproliferative activity against human HaCaT cells after 48 hrs by phase contrast microscopic analysis
Antihyperproliferative activity against human HaCaT cells after 48 hrs by phase contrast microscopic analysis
[PMID: 26840368]
HaCaT IC50
0.7 3
Compound: 5
Antihyperproliferative activity against human HaCaT cells assessed as inhibition of cell growth after 48 hrs by phase contrast microscopy
Antihyperproliferative activity against human HaCaT cells assessed as inhibition of cell growth after 48 hrs by phase contrast microscopy
[PMID: 24964246]
HaCaT IC50
0.6 3
Compound: anthralin
Antiproliferative activity (AA) against HaCaT keratinocytes.
Antiproliferative activity (AA) against HaCaT keratinocytes.
[PMID: 9276024]
HaCaT IC50
0.7 3
Compound: 3
Antihyperproliferative activity against human HaCaT cells after 48 hrs by phase contrast microscopy
Antihyperproliferative activity against human HaCaT cells after 48 hrs by phase contrast microscopy
[PMID: 22845014]
HaCaT IC50
0.7 3
Compound: Anthralin
Antiproliferative activity against growth of HaCaT cell was determined
Antiproliferative activity against growth of HaCaT cell was determined
[PMID: 11140730]
HaCaT IC50
0.2 3
Compound: anthralin
Antiproliferative activity against HaCaT cells
Antiproliferative activity against HaCaT cells
[PMID: 8201600]
HaCaT IC50
0.945 3
Compound: 1; DTR; dithranol
Antiproliferative activity against human HaCaT cells after 24 hrs by MTT assay
Antiproliferative activity against human HaCaT cells after 24 hrs by MTT assay
[PMID: 26515039]
HaCaT IC50
2.9 3
Compound: anthralin
Antiproliferative activity against human HaCaT cells assessed as [3H]thymidine incorporation after 24 hrs
Antiproliferative activity against human HaCaT cells assessed as [3H]thymidine incorporation after 24 hrs
[PMID: 15387642]
HaCaT IC50
0.7 3
Compound: 1; DTR; dithranol
Antiproliferative activity against human HaCaT cells
Antiproliferative activity against human HaCaT cells
[PMID: 26515039]
HaCaT IC50
0.7 3
Compound: Anthralin
Antiproliferative activity against human HaCaT cells
Antiproliferative activity against human HaCaT cells
[PMID: 10479319]
HEK293 GI50
0.5 3
Compound: 14
Growth inhibition of human NHEK cells after 72 hrs by neutral red uptake assay
Growth inhibition of human NHEK cells after 72 hrs by neutral red uptake assay
[PMID: 22944332]
HaCaT IC50
0.7 3
Compound: anthralin
Antiproliferative activity against human HaCaT cells
Antiproliferative activity against human HaCaT cells
[PMID: 10395495]
THP-1 GI50
54 3
Compound: 14
Growth inhibition of human THP1 cells after 72 hrs by Cell Titer 96 aqueous one solution assay
Growth inhibition of human THP1 cells after 72 hrs by Cell Titer 96 aqueous one solution assay
[PMID: 22944332]
U-937 GI50
5 3
Compound: 14
Growth inhibition of human U937 cells after 72 hrs by Cell Titer 96 aqueous one solution assay
Growth inhibition of human U937 cells after 72 hrs by Cell Titer 96 aqueous one solution assay
[PMID: 22944332]
HaCaT IC50
0.945 3
Compound: 1; DTR; dithranol
Antiproliferative activity against human HaCaT cells after 24 hrs by MTT assay
Antiproliferative activity against human HaCaT cells after 24 hrs by MTT assay
[PMID: 26515039]
HaCaT IC50
0.7 3
Compound: 1
Antiproliferative activity against human HaCaT cells after 48 hrs by phase contrast microscopy
Antiproliferative activity against human HaCaT cells after 48 hrs by phase contrast microscopy
[PMID: 20452101]
HaCaT IC50
0.7 3
Compound: 1
Antiproliferative activity against human HaCaT cells after 48 hrs by phase contrast microscopy
Antiproliferative activity against human HaCaT cells after 48 hrs by phase contrast microscopy
[PMID: 20828887]
HaCaT IC50
2.9 3
Compound: anthralin
Antiproliferative activity against human HaCaT cells assessed as [3H]thymidine incorporation after 24 hrs
Antiproliferative activity against human HaCaT cells assessed as [3H]thymidine incorporation after 24 hrs
[PMID: 15387642]
HaCaT IC50
0.7 3
Compound: 2, Anthralin
Antiproliferative activity against human HaCaT cells assessed as dispersed cells after 48 hrs by phase-contrast microscopic analysis
Antiproliferative activity against human HaCaT cells assessed as dispersed cells after 48 hrs by phase-contrast microscopic analysis
[PMID: 20850910]
HaCaT IC50
0.7 3
Compound: 1
Concentration required to inhibit 50% growth of HaCaT cells
Concentration required to inhibit 50% growth of HaCaT cells
[PMID: 11262091]
HaCaT IC50
0.7 3
Compound: 1
In vitro for antiproliferate activity against HaCaT cells
In vitro for antiproliferate activity against HaCaT cells
[PMID: 9873705]
HEK293 GI50
0.5 3
Compound: 14
Growth inhibition of human NHEK cells after 72 hrs by neutral red uptake assay
Growth inhibition of human NHEK cells after 72 hrs by neutral red uptake assay
[PMID: 22944332]
HaCaT GI50
0.7 3
Compound: 14
Growth inhibition of human HaCaT cells after 72 hrs by neutral red uptake assay
Growth inhibition of human HaCaT cells after 72 hrs by neutral red uptake assay
[PMID: 22944332]
PMNL IC50
37 3
Compound: anthralin
Inhibition of LTB4 biosynthesis in A-23187-stimulated bovine PMNL cells
Inhibition of LTB4 biosynthesis in A-23187-stimulated bovine PMNL cells
[PMID: 10395494]
THP-1 GI50
54 3
Compound: 14
Growth inhibition of human THP1 cells after 72 hrs by Cell Titer 96 aqueous one solution assay
Growth inhibition of human THP1 cells after 72 hrs by Cell Titer 96 aqueous one solution assay
[PMID: 22944332]
HaCaT IC50
0.2 3
Compound: anthralin
Antiproliferative activity against HaCaT cells
Antiproliferative activity against HaCaT cells
[PMID: 8201600]
U-937 GI50
5 3
Compound: 14
Growth inhibition of human U937 cells after 72 hrs by Cell Titer 96 aqueous one solution assay
Growth inhibition of human U937 cells after 72 hrs by Cell Titer 96 aqueous one solution assay
[PMID: 22944332]
HaCaT IC50
0.6 3
Compound: 1/ Anthralin
Antiproliferative (inhibition of cell growth) activity against HaCaT cells (human keratinocyte line)
Antiproliferative (inhibition of cell growth) activity against HaCaT cells (human keratinocyte line)
[PMID: 9371243]
HaCaT IC50
0.6 3
Compound: anthralin
Antiproliferative activity (AA) against HaCaT keratinocytes.
Antiproliferative activity (AA) against HaCaT keratinocytes.
[PMID: 9276024]
HaCaT IC50
0.7 3
Compound: 1
Concentration required to inhibit 50% growth of HaCaT cells
Concentration required to inhibit 50% growth of HaCaT cells
[PMID: 11262091]
HaCaT IC50
0.7 3
Compound: 1
Antiproliferative activity against human HaCaT cells after 48 hrs by phase contrast microscopy
Antiproliferative activity against human HaCaT cells after 48 hrs by phase contrast microscopy
[PMID: 20452101]
HaCaT IC50
0.7 3
Compound: 1
Antiproliferative activity against human HaCaT cells after 48 hrs by phase contrast microscopy
Antiproliferative activity against human HaCaT cells after 48 hrs by phase contrast microscopy
[PMID: 20828887]
HaCaT IC50
0.7 3
Compound: 1
In vitro for antiproliferate activity against HaCaT cells
In vitro for antiproliferate activity against HaCaT cells
[PMID: 9873705]
HaCaT IC50
0.7 3
Compound: 1; DTR; dithranol
Antiproliferative activity against human HaCaT cells
Antiproliferative activity against human HaCaT cells
[PMID: 26515039]
HaCaT IC50
0.7 3
Compound: 2
Antihyperproliferative activity against human HaCaT cells after 48 hrs by phase contrast microscopic analysis
Antihyperproliferative activity against human HaCaT cells after 48 hrs by phase contrast microscopic analysis
[PMID: 26840368]
HaCaT IC50
0.7 3
Compound: 2, Anthralin
Antiproliferative activity against human HaCaT cells assessed as dispersed cells after 48 hrs by phase-contrast microscopic analysis
Antiproliferative activity against human HaCaT cells assessed as dispersed cells after 48 hrs by phase-contrast microscopic analysis
[PMID: 20850910]
HaCaT IC50
0.7 3
Compound: 3
Antihyperproliferative activity against human HaCaT cells after 48 hrs by phase contrast microscopy
Antihyperproliferative activity against human HaCaT cells after 48 hrs by phase contrast microscopy
[PMID: 22845014]
HaCaT IC50
0.7 3
Compound: 5
Antihyperproliferative activity against human HaCaT cells assessed as inhibition of cell growth after 48 hrs by phase contrast microscopy
Antihyperproliferative activity against human HaCaT cells assessed as inhibition of cell growth after 48 hrs by phase contrast microscopy
[PMID: 24964246]
HaCaT IC50
0.7 3
Compound: Anthralin
Antiproliferative activity against human HaCaT cells
Antiproliferative activity against human HaCaT cells
[PMID: 10479319]
HaCaT IC50
0.7 3
Compound: Anthralin
Antiproliferative activity against growth of HaCaT cell was determined
Antiproliferative activity against growth of HaCaT cell was determined
[PMID: 11140730]
HaCaT IC50
0.7 3
Compound: Anthralin
Antihyperproliferative activity against human HaCaT cells after 48 hrs by MTT assay
Antihyperproliferative activity against human HaCaT cells after 48 hrs by MTT assay
[PMID: 28410493]
HaCaT IC50
0.7 3
Compound: anthralin
Antiproliferative activity against human HaCaT cells
Antiproliferative activity against human HaCaT cells
[PMID: 10395495]
HaCaT IC50
0.945 3
Compound: 1; DTR; dithranol
Antiproliferative activity against human HaCaT cells after 24 hrs by MTT assay
Antiproliferative activity against human HaCaT cells after 24 hrs by MTT assay
[PMID: 26515039]
HaCaT IC50
2.9 3
Compound: anthralin
Antiproliferative activity against human HaCaT cells assessed as [3H]thymidine incorporation after 24 hrs
Antiproliferative activity against human HaCaT cells assessed as [3H]thymidine incorporation after 24 hrs
[PMID: 15387642]
HaCaT IC50
3.52 3
Compound: Anthralin
Antiproliferative activity against human HaCaT cells incubated for 48 hrs by MTT assay
Antiproliferative activity against human HaCaT cells incubated for 48 hrs by MTT assay
[PMID: 39151117]
PMNL IC50
37 3
Compound: anthralin
Inhibition of LTB4 biosynthesis in A-23187-stimulated bovine PMNL cells
Inhibition of LTB4 biosynthesis in A-23187-stimulated bovine PMNL cells
[PMID: 10395494]
THP-1 GI50
54 3
Compound: 14
Growth inhibition of human THP1 cells after 72 hrs by Cell Titer 96 aqueous one solution assay
Growth inhibition of human THP1 cells after 72 hrs by Cell Titer 96 aqueous one solution assay
[PMID: 22944332]
U-937 GI50
5 3
Compound: 14
Growth inhibition of human U937 cells after 72 hrs by Cell Titer 96 aqueous one solution assay
Growth inhibition of human U937 cells after 72 hrs by Cell Titer 96 aqueous one solution assay
[PMID: 22944332]
体外実験

Dithranol (1.1 μM) inhibits replication in T98G cells by 50%[2].
? Dithranol (0.1-0.5 μg/mL; 2-4 hours) increases the mRNA production of interleukin-10 receptors in a concentration dependent manner in HaCaT cells[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

RT-PCR[3]

Cell Line: HaCaT cells
Concentration: 0.1 μg/mL, 0.25 μg/mL, 0.5 μg/mL
Incubation Time: 2 hours, 4 hours
Result: Increased the mRNA production of interleukin-10 receptors in HaCaT cells.
臨床実験
分子量

226.23

分子式

C14H10O3

CAS 番号
Appearance

Solid

Color

Yellow to orange

SMILES

O=C1C2=C(C=CC=C2O)CC3=CC=CC(O)=C13

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
溶剤 & 溶解度
体外: 

DMSO : 20 mg/mL (88.41 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.4203 mL 22.1014 mL 44.2028 mL
5 mM 0.8841 mL 4.4203 mL 8.8406 mL
10 mM 0.4420 mL 2.2101 mL 4.4203 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます:C1V1 = C2V2

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Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 1 mg/mL (4.42 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 1 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 1 mg/mL (4.42 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 1 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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(per animal)

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
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%
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Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション

純度: ≥98.0%

参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.4203 mL 22.1014 mL 44.2028 mL 110.5070 mL
5 mM 0.8841 mL 4.4203 mL 8.8406 mL 22.1014 mL
10 mM 0.4420 mL 2.2101 mL 4.4203 mL 11.0507 mL
15 mM 0.2947 mL 1.4734 mL 2.9469 mL 7.3671 mL
20 mM 0.2210 mL 1.1051 mL 2.2101 mL 5.5254 mL
25 mM 0.1768 mL 0.8841 mL 1.7681 mL 4.4203 mL
30 mM 0.1473 mL 0.7367 mL 1.4734 mL 3.6836 mL
40 mM 0.1105 mL 0.5525 mL 1.1051 mL 2.7627 mL
50 mM 0.0884 mL 0.4420 mL 0.8841 mL 2.2101 mL
60 mM 0.0737 mL 0.3684 mL 0.7367 mL 1.8418 mL
80 mM 0.0553 mL 0.2763 mL 0.5525 mL 1.3813 mL
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  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます:C1V1 = C2V2

濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
× = ×
C1   V1   C2   V2
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製品名:
Dithranol
製品番号:
HY-B0738
数量:
MCE 日本正規代理店: