Erastin (Synonyms: エラスチン)

Name: Erastin
Brand: MedChemExpress
SKU: HY-15763
Rating: 5.0 (805 reviews)

製品番号: HY-15763 純度: 99.91%: Data Sheet SDS COA 取扱説明書
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Erastin is a ferroptosis inducer. Erastin exhibits the mechanism of ferroptosis induction related to ROS and iron-dependent signaling. Erastin inhibits voltage-dependent anion channels (VDAC2/VDAC3) and accelerates oxidation, leading to the accumulation of endogenous reactive oxygen species. Erastin also disrupts mitochondrial permeability transition pore (mPTP) with anti-tumor activity. Furthermore, Erastin can block the uptake of cystine mediated by SLC7A11 and also spares UMRC6-EV and -C91A cells from disulfidptosis under glucose starvation.

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CAS 番号 : 571203-78-6

容量	価格（税別）	在庫状況	数量
1 mg	$50	在庫あり	Estimated Time of Arrival: December 31
1 mg * 5	$115	在庫あり	Estimated Time of Arrival: December 31
1 mg * 10	$190	在庫あり	Estimated Time of Arrival: December 31

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カスタマーレビュー

Based on 805 publication(s) in Google Scholar

Other Forms of Erastin:

Imidazole ketone erastin 在庫あり

顧客検証

Bio/Physico-chemical Assay

Cell Imaging/Staining

RT-PCR

Cell Proliferation/Viability Assay

: Erastin purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 May 21:e2505359. [Abstract]; Intracellular ROS was assessed using the DCFH-DA probe after being treated with 30 µM erastin or 500 µM FAC (Ammonium iron(III) citrate) for 4 h.

: Erastin purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 May 21:e2505359. [Abstract]; Intracellular Fe2+ levels under 30 µM erastin or 500 µM FAC (Ammonium iron(III) citrate) treatment for 4 h were detected with the RhoNox-1(1μM; 1 h) fluorescent probe.

: Erastin purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 May 21:e2505359. [Abstract]; Representative images of ferroptotic cells among iPSC-RPE cells, De-iPSC-RPE cells, and iRPE cells treated with 30 µM erastin or 500 µM FAC (Ammonium iron(III) citrate) for 2 days.

: Erastin purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2024 Nov;44(11):1261-1286. [Abstract]; Erastin (0.1-5 µM; 48 h) induces cell death (ferroptosis) in both 4T1-LM3 and E0771-LM3 cells.

: Erastin purchased from MedChemExpress. Usage Cited in: J Pharm Anal. 2025 May;15(5):101092.; The protein levels of MMP13, ADAMTS4, COL II and Aggrecan in chondrocytes treated with ferroptosis inducers erastin (10 μM), vitamin K1 (VK1;10 μM) and ferroptosis inhibitor Ferrostatin-1 (Fer-1; 1 μM) for 24 h were determined by Western blotting assay

: Erastin purchased from MedChemExpress. Usage Cited in: J Pharm Anal. 2025 May;15(5):101092.; The mRNA levels of catabolic enzymes (Mmp13 and Adamts4) and matrix components (Col2a1 and Acan) in chondrocytes treated with ferroptosis inducers erastin (10 μM), vitamin K1 (VK1; 10 μM) and ferroptosis inhibitor Ferrostatin-1 (Fer-1; 1 μM) for 24 h were determined by RT-qPCR.

: Erastin purchased from MedChemExpress. Usage Cited in: J Pharm Anal. 2025 May;15(5):101092.; Immunofluorescence of MMP13, ADAMTS4, COL II and Aggrecan in chondrocytes treated with ferroptosis inducers erastin (10 μM), vitamin K1 (VK1; 10 μM) and ferroptosis inhibitor Ferrostatin-1 (Fer-1; 1 μM) for 24 h.

: Erastin purchased from MedChemExpress. Usage Cited in: ACS Nano. 2023 Nov 28;17(22):22240-22258. [Abstract]; Percentage of cell viability in HCC samples (paired sorafenib-sensitive and sorafenib-resistant HepG2, PLC/PRF/5, and PDO were included) treated with 3 μM erastin.

: Erastin purchased from MedChemExpress. Usage Cited in: Gene. 2023 Jul 15:873:147468. [Abstract]; Erastin (8 μM; 48 h) greatly induces apoptosis and cell death in AGS and BGC-823 cells.

: Erastin purchased from MedChemExpress. Usage Cited in: Gene. 2023 Jul 15:873:147468. [Abstract]; Erastin (8, 16 μM; 48 h) reduces the expression of xCT in AGS and BGC-823 cells.

: Erastin purchased from MedChemExpress. Usage Cited in: Nat Cell Biol. 2022 Feb;24(2):168-180. [Abstract]; Cell survival in MCF10A-RAS cells cultured on stiff or soft Matrigel and treated for 48 h with Erastin (ERA) (10-30 μM).

: Erastin purchased from MedChemExpress. Usage Cited in: Redox Biol. 2022 Aug;54:102382. [Abstract]; After treatment with Ferrostatin-1 (Fer-1, 5 μM) and GW4064 (1 μM) for 2 h, HK2 cells are treated with Erastin (5 μM) for another 24 h. Protein levels of GPX4 are detected by immunoblotting.

: Erastin purchased from MedChemExpress. Usage Cited in: Redox Biol. 2022 Aug;54:102382. [Abstract]; After treatment with Ferrostatin-1 (Fer-1, 5 μM) and GW4064 (1 μM) for 2 h, HK2 cells are treated with Erastin (5 μM) for another 24 h. After treatment with Fer-1 and GW4064, the cells are incubated with C11-BODIPY 581/591 (2 μM) and Hoechst 33258 for counterstaining; the images were then immediately visualized by confocal microscopy.

: Erastin purchased from MedChemExpress. Usage Cited in: Clin Transl Med. 2022 Apr;12(4):e752. [Abstract]; Representative images of intracellular mitochondrial superoxide level in SW480 and HT29 cells subjected to the stable knockdown of LINC001606 or stably overexpressing LINC001606 after treatment of DMSO, Erastin (10 μM) and RSL3 (2 μM) for 48 h.

: Erastin purchased from MedChemExpress. Usage Cited in: Cell Death Differ. 2020 Sep;27(9):2635-2650. [Abstract]; Erastin (10 μM; tail-injected intravenously; for 20 days) treatment of I/R mice significantly increases the protein/mRNA levels of TF and significantly decreased the percentage GSH and protein/mRNA levels of FTH1 and GPX4, but these effects are reversed by Liproxstatin-1 treatment.

: Erastin purchased from MedChemExpress. Usage Cited in: Cell Death Differ. 2020 Sep;27(9):2635-2650. [Abstract]; H&E and Masson’s staining of lung tissues show that Erastin (10 μM; tail-injected intravenously; for 20 days) aggravated edema, atelectasis, necrosis, alveolar and interstitial inflammation, and pulmonary fibrosis in I/R mice compared with sham mice, but these effects are reversed in I/R + erastin mice treated with Liproxstatin-1.

: Erastin purchased from MedChemExpress. Usage Cited in: Cell Death Differ. 2021 Apr;28(4):1222-1236. [Abstract]; Western blot analysis of the protein expression levels of BCAT2, AMPK, and pAMPK(T172) in Aspc-1, and HepG2 cells treated with Erastin (20 or 10 μM) Vin the absence or presence of AICAR (AMPK activator, 2 mmol/L) and Compound C (AMPK inhibitor, 1 μmol/L).

: Erastin purchased from MedChemExpress. Usage Cited in: Neurotherapeutics. 2020 Oct;17(4):1796-1812. [Abstract]; The effect of an inducer or inhibitor of ferroptosis on PC-12 cells. PC-12 cells are treated with 40 μM 6-OHDA in combination with an inducer (1 μM Erastin) of ferroptosis for 24 h. The expression of GPX4, TH, and FTH1 is significantly reduced in cells treated with the combination of erastin and 6-OHDA compared with those treated with 6-OHDA alone.

: Erastin purchased from MedChemExpress. Usage Cited in: Eur J Pharmacol. 2020 Dec 5;888:173574. [Abstract]; With the various dosage of ferroptosis inducer Erastin treatment for 24 h, the cell viability is determined by SRB method.

VDAC アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

VDAC1 VDAC2 VDAC3

生物活性
純度とドキュメンテーション
参考文献
カスタマーレビュー

製品説明

IC₅₀ & Target

VDAC2

VDAC3

Cellular Effect

Cell Line	Type	Value	Description	References
A673	EC₅₀	30 3 Compound: erastin	Induction of cell death in human A673 cells Induction of cell death in human A673 cells	[PMID: 17568748]
BJ	IC₅₀	0.9 3 Compound: erastin	Induction of cell death in human BJ cells expressing TERT, LT, ST and RAS G12V mutant genes cells in presence of PD-98059 by trypan blue exclusion method Induction of cell death in human BJ cells expressing TERT, LT, ST and RAS G12V mutant genes cells in presence of PD-98059 by trypan blue exclusion method	[PMID: 17568748]
BJ	EC₅₀	6 3 Compound: erastin	Induction of cell death in human BJ cells expressing TERT, LT, ST and RAS G12V mutant genes Induction of cell death in human BJ cells expressing TERT, LT, ST and RAS G12V mutant genes	[PMID: 17568748]
BJ	IC₅₀	0.9 3 Compound: erastin	Induction of cell death in human BJ cells expressing TERT, LT, ST and RAS G12V mutant genes cells in presence of MEK inhibitor 2 by trypan blue exclusion method Induction of cell death in human BJ cells expressing TERT, LT, ST and RAS G12V mutant genes cells in presence of MEK inhibitor 2 by trypan blue exclusion method	[PMID: 17568748]
BJ	EC₅₀	10 3 Compound: erastin	Induction of cell death in TERT expressing human BJ cells Induction of cell death in TERT expressing human BJ cells	[PMID: 17568748]
BJ	IC₅₀	2 3 Compound: erastin	Induction of cell death in human BJ cells expressing TERT, LT, ST and RAS G12V mutant genes cells in presence of MEK 1/2 inhibitor by trypan blue exclusion method Induction of cell death in human BJ cells expressing TERT, LT, ST and RAS G12V mutant genes cells in presence of MEK 1/2 inhibitor by trypan blue exclusion method	[PMID: 17568748]
BJ	IC₅₀	2 3 Compound: erastin	Induction of cell death in human BJ cells expressing TERT, LT, ST and RAS G12V mutant genes cells in presence of MEK 1/2 inhibitor by trypan blue exclusion method Induction of cell death in human BJ cells expressing TERT, LT, ST and RAS G12V mutant genes cells in presence of MEK 1/2 inhibitor by trypan blue exclusion method	[PMID: 17568748]
BJ	IC₅₀	2.6 3 Compound: erastin	Induction of cell death in human BJ cells expressing TERT, LT, ST and RAS G12V mutant genes in presence of MEK inhibitor by trypan blue exclusion method Induction of cell death in human BJ cells expressing TERT, LT, ST and RAS G12V mutant genes in presence of MEK inhibitor by trypan blue exclusion method	[PMID: 17568748]
BJ	IC₅₀	0.9 3 Compound: erastin	Induction of cell death in human BJ cells expressing TERT, LT, ST and RAS G12V mutant genes cells in presence of MEK inhibitor 2 by trypan blue exclusion method Induction of cell death in human BJ cells expressing TERT, LT, ST and RAS G12V mutant genes cells in presence of MEK inhibitor 2 by trypan blue exclusion method	[PMID: 17568748]
BJ	IC₅₀	31.2 3 Compound: erastin	Induction of cell death in human BJ cells expressing TERT, LT, ST and RAS G12V mutant genes in presence of U0126 by trypan blue exclusion method Induction of cell death in human BJ cells expressing TERT, LT, ST and RAS G12V mutant genes in presence of U0126 by trypan blue exclusion method	[PMID: 17568748]
Calu-1	IC₅₀	4 3 Compound: erastin	Inhibition of human Calu1 cells expressing KRAS with activating mutations by trypan blue exclusion assay Inhibition of human Calu1 cells expressing KRAS with activating mutations by trypan blue exclusion assay	[PMID: 17568748]
A673	EC₅₀	30 3 Compound: erastin	Induction of cell death in human A673 cells Induction of cell death in human A673 cells	[PMID: 17568748]
BJ	IC₅₀	2.6 3 Compound: erastin	Induction of cell death in human BJ cells expressing TERT, LT, ST and RAS G12V mutant genes in presence of MEK inhibitor by trypan blue exclusion method Induction of cell death in human BJ cells expressing TERT, LT, ST and RAS G12V mutant genes in presence of MEK inhibitor by trypan blue exclusion method	[PMID: 17568748]
BJ	IC₅₀	31.2 3 Compound: erastin	Induction of cell death in human BJ cells expressing TERT, LT, ST and RAS G12V mutant genes in presence of U0126 by trypan blue exclusion method Induction of cell death in human BJ cells expressing TERT, LT, ST and RAS G12V mutant genes in presence of U0126 by trypan blue exclusion method	[PMID: 17568748]
B16	IC₅₀	5 3 Compound: Erastin	Cytotoxicity against mouse B16 cells assessed as inhibition of cell viability measured after 24 hrs by MTT assay Cytotoxicity against mouse B16 cells assessed as inhibition of cell viability measured after 24 hrs by MTT assay	[PMID: 38306267]
CCF-STTG1	IC₅₀	200 1 Compound: ERA	Inhibition of Xct in human CCF-STTG1 cells assessed as glutamate release after 2 hrs by fluorometry Inhibition of Xct in human CCF-STTG1 cells assessed as glutamate release after 2 hrs by fluorometry	[PMID: 26231156]
C-33-A	EC₅₀	0.6 3 Compound: erastin	Induction of cell death in human C33A cells Induction of cell death in human C33A cells	[PMID: 17568748]
HT-1080	IC₅₀	1 3 Compound: erastin	Induction of cell death in human HT1080 cells in presence of PD-98059 by trypan blue exclusion method Induction of cell death in human HT1080 cells in presence of PD-98059 by trypan blue exclusion method	[PMID: 17568748]
Calu-1	IC₅₀	0.14 3 Compound: 4	Induction of ferroptosis in human Calu-1 cells assessed as inhibition of (cystine-glutamate antiporter) system Xc- mediated cystine transport Induction of ferroptosis in human Calu-1 cells assessed as inhibition of (cystine-glutamate antiporter) system Xc- mediated cystine transport	[PMID: 36332549]
HT-1080	IC₅₀	1.3 3 Compound: erastin	Induction of cell death in human HT1080 cells in presence of MEK inhibitor 2 by trypan blue exclusion method Induction of cell death in human HT1080 cells in presence of MEK inhibitor 2 by trypan blue exclusion method	[PMID: 17568748]
Calu-1	IC₅₀	4 3 Compound: erastin	Inhibition of human Calu1 cells expressing KRAS with activating mutations by trypan blue exclusion assay Inhibition of human Calu1 cells expressing KRAS with activating mutations by trypan blue exclusion assay	[PMID: 17568748]
HT-1080	IC₅₀	2.5 3 Compound: erastin	Induction of cell death in human HT1080 cells in presence of MEK inhibitor by trypan blue exclusion method Induction of cell death in human HT1080 cells in presence of MEK inhibitor by trypan blue exclusion method	[PMID: 17568748]
HeLa	EC₅₀	0.6 3 Compound: erastin	Induction of cell death in human HeLa cells Induction of cell death in human HeLa cells	[PMID: 17568748]
HT-1080	IC₅₀	3.4 3 Compound: erastin	Induction of cell death in human HT1080 cells in presence of U0126 by trypan blue exclusion method Induction of cell death in human HT1080 cells in presence of U0126 by trypan blue exclusion method	[PMID: 17568748]
HOS	EC₅₀	17 3 Compound: erastin	Induction of cell death in human HOS cells Induction of cell death in human HOS cells	[PMID: 17568748]
HT-1080	IC₅₀	6 3 Compound: erastin	Induction of cell death in human HT1080 cells in presence of MEK 1/2 inhibitor by trypan blue exclusion method Induction of cell death in human HT1080 cells in presence of MEK 1/2 inhibitor by trypan blue exclusion method	[PMID: 17568748]
HT-1080	EC₅₀	2 3 Compound: erastin	Induction of cell death in human HT1080 cells Induction of cell death in human HT1080 cells	[PMID: 17568748]
BJ	EC₅₀	10 3 Compound: erastin	Induction of cell death in TERT expressing human BJ cells Induction of cell death in TERT expressing human BJ cells	[PMID: 17568748]
HT-1080	IC₅₀	6 3 Compound: erastin	Induction of cell death in human HT1080 cells in presence of MEK 1/2 inhibitor by trypan blue exclusion method Induction of cell death in human HT1080 cells in presence of MEK 1/2 inhibitor by trypan blue exclusion method	[PMID: 17568748]
BJ	EC₅₀	6 3 Compound: erastin	Induction of cell death in human BJ cells expressing TERT, LT, ST and RAS G12V mutant genes Induction of cell death in human BJ cells expressing TERT, LT, ST and RAS G12V mutant genes	[PMID: 17568748]
HT-1080	IC₅₀	1.3 3 Compound: erastin	Induction of cell death in human HT1080 cells in presence of MEK inhibitor 2 by trypan blue exclusion method Induction of cell death in human HT1080 cells in presence of MEK inhibitor 2 by trypan blue exclusion method	[PMID: 17568748]
BJ	IC₅₀	0.9 3 Compound: erastin	Induction of cell death in human BJ cells expressing TERT, LT, ST and RAS G12V mutant genes cells in presence of MEK inhibitor 2 by trypan blue exclusion method Induction of cell death in human BJ cells expressing TERT, LT, ST and RAS G12V mutant genes cells in presence of MEK inhibitor 2 by trypan blue exclusion method	[PMID: 17568748]
BJ	IC₅₀	0.9 3 Compound: erastin	Induction of cell death in human BJ cells expressing TERT, LT, ST and RAS G12V mutant genes cells in presence of PD-98059 by trypan blue exclusion method Induction of cell death in human BJ cells expressing TERT, LT, ST and RAS G12V mutant genes cells in presence of PD-98059 by trypan blue exclusion method	[PMID: 17568748]
HT-1080	IC₅₀	2.5 3 Compound: erastin	Induction of cell death in human HT1080 cells in presence of MEK inhibitor by trypan blue exclusion method Induction of cell death in human HT1080 cells in presence of MEK inhibitor by trypan blue exclusion method	[PMID: 17568748]
HT-1080	IC₅₀	1 3 Compound: erastin	Induction of cell death in human HT1080 cells in presence of PD-98059 by trypan blue exclusion method Induction of cell death in human HT1080 cells in presence of PD-98059 by trypan blue exclusion method	[PMID: 17568748]
BJ	IC₅₀	2 3 Compound: erastin	Induction of cell death in human BJ cells expressing TERT, LT, ST and RAS G12V mutant genes cells in presence of MEK 1/2 inhibitor by trypan blue exclusion method Induction of cell death in human BJ cells expressing TERT, LT, ST and RAS G12V mutant genes cells in presence of MEK 1/2 inhibitor by trypan blue exclusion method	[PMID: 17568748]
HT-1080	IC₅₀	3.4 3 Compound: erastin	Induction of cell death in human HT1080 cells in presence of U0126 by trypan blue exclusion method Induction of cell death in human HT1080 cells in presence of U0126 by trypan blue exclusion method	[PMID: 17568748]
BJ	IC₅₀	2.6 3 Compound: erastin	Induction of cell death in human BJ cells expressing TERT, LT, ST and RAS G12V mutant genes in presence of MEK inhibitor by trypan blue exclusion method Induction of cell death in human BJ cells expressing TERT, LT, ST and RAS G12V mutant genes in presence of MEK inhibitor by trypan blue exclusion method	[PMID: 17568748]
HT-1080	IC₅₀	0.2 3 Compound: 4	Induction of ferroptosis in human HT-1080 cells assessed as inhibition of (cystine-glutamate antiporter) system Xc- mediated cystine transport Induction of ferroptosis in human HT-1080 cells assessed as inhibition of (cystine-glutamate antiporter) system Xc- mediated cystine transport	[PMID: 36332549]
BJ	IC₅₀	31.2 3 Compound: erastin	Induction of cell death in human BJ cells expressing TERT, LT, ST and RAS G12V mutant genes in presence of U0126 by trypan blue exclusion method Induction of cell death in human BJ cells expressing TERT, LT, ST and RAS G12V mutant genes in presence of U0126 by trypan blue exclusion method	[PMID: 17568748]
HT-1080	IC₅₀	1.52 3 Compound: Erastin	Lethality in human HT-1080 cells assessed as inhibition of cell viability incubated for 24 hrs by MTT assay Lethality in human HT-1080 cells assessed as inhibition of cell viability incubated for 24 hrs by MTT assay	[PMID: 36410168]
LNCaP	EC₅₀	6 3 Compound: erastin	Induction of cell death in human LNCaP cells Induction of cell death in human LNCaP cells	[PMID: 17568748]
C-33-A	EC₅₀	0.6 3 Compound: erastin	Induction of cell death in human C33A cells Induction of cell death in human C33A cells	[PMID: 17568748]
MES-SA	EC₅₀	3 3 Compound: erastin	Induction of cell death in human MES-SA cells Induction of cell death in human MES-SA cells	[PMID: 17568748]
CCF-STTG1	IC₅₀	200 1 Compound: ERA	Inhibition of Xct in human CCF-STTG1 cells assessed as glutamate release after 2 hrs by fluorometry Inhibition of Xct in human CCF-STTG1 cells assessed as glutamate release after 2 hrs by fluorometry	[PMID: 26231156]
CCF-STTG1	IC₅₀	200 1 Compound: ERA	Inhibition of Xct in human CCF-STTG1 cells assessed as glutamate release after 2 hrs by fluorometry Inhibition of Xct in human CCF-STTG1 cells assessed as glutamate release after 2 hrs by fluorometry	[PMID: 26231156]
Calu-1	IC₅₀	0.14 3 Compound: 4	Induction of ferroptosis in human Calu-1 cells assessed as inhibition of (cystine-glutamate antiporter) system Xc- mediated cystine transport Induction of ferroptosis in human Calu-1 cells assessed as inhibition of (cystine-glutamate antiporter) system Xc- mediated cystine transport	[PMID: 36332549]
SJSA-1	EC₅₀	12 3 Compound: erastin	Induction of cell death in human SJSA1 cells Induction of cell death in human SJSA1 cells	[PMID: 17568748]
Calu-1	IC₅₀	4 3 Compound: erastin	Inhibition of human Calu1 cells expressing KRAS with activating mutations by trypan blue exclusion assay Inhibition of human Calu1 cells expressing KRAS with activating mutations by trypan blue exclusion assay	[PMID: 17568748]
SK-ES1	EC₅₀	7 3 Compound: erastin	Induction of cell death in human SK-ES-1 cells Induction of cell death in human SK-ES-1 cells	[PMID: 17568748]
SK-LMS-1	EC₅₀	6 3 Compound: erastin	Induction of cell death in human SKLMS1 cells Induction of cell death in human SKLMS1 cells	[PMID: 17568748]
SK-N-MC	EC₅₀	10 3 Compound: erastin	Induction of cell death in human SK-N-MC cells Induction of cell death in human SK-N-MC cells	[PMID: 17568748]
SVR	EC₅₀	2.5 3 Compound: erastin	Induction of cell death in human SVR cells Induction of cell death in human SVR cells	[PMID: 17568748]
HOS	EC₅₀	17 3 Compound: erastin	Induction of cell death in human HOS cells Induction of cell death in human HOS cells	[PMID: 17568748]
TC-32	EC₅₀	8 3 Compound: erastin	Induction of cell death in human TC32 cells Induction of cell death in human TC32 cells	[PMID: 17568748]
U2OS	EC₅₀	6 3 Compound: erastin	Induction of cell death in human U2OS cells Induction of cell death in human U2OS cells	[PMID: 17568748]
U-937	EC₅₀	10 3 Compound: erastin	Induction of cell death in human U937 cells Induction of cell death in human U937 cells	[PMID: 17568748]
HT-1080	EC₅₀	2 3 Compound: erastin	Induction of cell death in human HT1080 cells Induction of cell death in human HT1080 cells	[PMID: 17568748]
HT-1080	IC₅₀	0.2 3 Compound: 4	Induction of ferroptosis in human HT-1080 cells assessed as inhibition of (cystine-glutamate antiporter) system Xc- mediated cystine transport Induction of ferroptosis in human HT-1080 cells assessed as inhibition of (cystine-glutamate antiporter) system Xc- mediated cystine transport	[PMID: 36332549]
HT-1080	IC₅₀	1 3 Compound: erastin	Induction of cell death in human HT1080 cells in presence of PD-98059 by trypan blue exclusion method Induction of cell death in human HT1080 cells in presence of PD-98059 by trypan blue exclusion method	[PMID: 17568748]
HT-1080	IC₅₀	1.3 3 Compound: erastin	Induction of cell death in human HT1080 cells in presence of MEK inhibitor 2 by trypan blue exclusion method Induction of cell death in human HT1080 cells in presence of MEK inhibitor 2 by trypan blue exclusion method	[PMID: 17568748]
HT-1080	IC₅₀	1.52 3 Compound: Erastin	Lethality in human HT-1080 cells assessed as inhibition of cell viability incubated for 24 hrs by MTT assay Lethality in human HT-1080 cells assessed as inhibition of cell viability incubated for 24 hrs by MTT assay	[PMID: 36410168]
HT-1080	IC₅₀	2.5 3 Compound: erastin	Induction of cell death in human HT1080 cells in presence of MEK inhibitor by trypan blue exclusion method Induction of cell death in human HT1080 cells in presence of MEK inhibitor by trypan blue exclusion method	[PMID: 17568748]
HT-1080	IC₅₀	3.4 3 Compound: erastin	Induction of cell death in human HT1080 cells in presence of U0126 by trypan blue exclusion method Induction of cell death in human HT1080 cells in presence of U0126 by trypan blue exclusion method	[PMID: 17568748]
HT-1080	IC₅₀	6 3 Compound: erastin	Induction of cell death in human HT1080 cells in presence of MEK 1/2 inhibitor by trypan blue exclusion method Induction of cell death in human HT1080 cells in presence of MEK 1/2 inhibitor by trypan blue exclusion method	[PMID: 17568748]
HT-29	IC₅₀	20.89 3 Compound: Erastin	Antiproliferative activity against human HT-29 cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay Antiproliferative activity against human HT-29 cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay	[PMID: 38422700]
HeLa	EC₅₀	0.6 3 Compound: erastin	Induction of cell death in human HeLa cells Induction of cell death in human HeLa cells	[PMID: 17568748]
LNCaP	EC₅₀	6 3 Compound: erastin	Induction of cell death in human LNCaP cells Induction of cell death in human LNCaP cells	[PMID: 17568748]
MC-38	IC₅₀	0.5 3 Compound: Erastin	Cytotoxicity against mouse MC-38 cells assessed as reduction in cell viability incubated for 24 hrs by MTS assay Cytotoxicity against mouse MC-38 cells assessed as reduction in cell viability incubated for 24 hrs by MTS assay	[PMID: 38306267]
MES-SA	EC₅₀	3 3 Compound: erastin	Induction of cell death in human MES-SA cells Induction of cell death in human MES-SA cells	[PMID: 17568748]
SJSA-1	EC₅₀	12 3 Compound: erastin	Induction of cell death in human SJSA1 cells Induction of cell death in human SJSA1 cells	[PMID: 17568748]
SK-ES1	EC₅₀	7 3 Compound: erastin	Induction of cell death in human SK-ES-1 cells Induction of cell death in human SK-ES-1 cells	[PMID: 17568748]
SK-LMS-1	EC₅₀	6 3 Compound: erastin	Induction of cell death in human SKLMS1 cells Induction of cell death in human SKLMS1 cells	[PMID: 17568748]
SK-N-MC	EC₅₀	10 3 Compound: erastin	Induction of cell death in human SK-N-MC cells Induction of cell death in human SK-N-MC cells	[PMID: 17568748]
SVR	EC₅₀	2.5 3 Compound: erastin	Induction of cell death in human SVR cells Induction of cell death in human SVR cells	[PMID: 17568748]
TC-32	EC₅₀	8 3 Compound: erastin	Induction of cell death in human TC32 cells Induction of cell death in human TC32 cells	[PMID: 17568748]
U-937	EC₅₀	10 3 Compound: erastin	Induction of cell death in human U937 cells Induction of cell death in human U937 cells	[PMID: 17568748]
U2OS	EC₅₀	6 3 Compound: erastin	Induction of cell death in human U2OS cells Induction of cell death in human U2OS cells	[PMID: 17568748]

体外実験

Erastin (10 μM; 24 h) triggers ferroptosis in ectopic endometrial stromal cells (EESCs), and increases the total ROS level at 9 h^[1].
Erastin shorts mitochondria and increases membrane density in EESCs^[1].
Erastin (10 μM; 9 h) decreases the mRNA expression levels of iron-related proteins, such FPN (iron exporter) in EESCs. However, FPN overexpression significantly inhibits erastin-induced ferroptosis in EESCs^[1].
Erastin (10 μM; 24 h) induces mitochondrial permeability transition pore (mPTP) opening in HT-29 colorectal cancer cells^[2].
Erastin (30 μM; 72 h) significantly inhibits the growth of HT-29 colorectal cancer cells^[2].
The molecular mechanism by which Erastin induces ferroptosis is related to genes regulating iron or mitochondrial fatty acid metabolism. Includes ribosomal protein L8, iron response element binding protein 2 (IREB2), ATP synthase F0 complex subunit C3, citrate synthase, tetrapeptide repeat domain 35, and acyl-CoA synthetase family member 2 (ACSF2)^[3].
Note:
1. Different cell lines may have different sensitivity to a same compound. As reported, A549, HCT116, HepG2, H1299 cells may be insensitive to Erastin^[3]^[4]^[5].
2. Erastin is unstable in solution. Freshly prepared is recommended.

Ferroptosis-sensitive Cell Lines

Ferroptosis-sensitive Cells	Test Conditions
SKOV3^[6]	5-20 μM; 1-7 days
OVCA429^[6]	5-20 μM; 1-7 days
MCF10A-RAS^[7]	0-30 μM; 48 h
HT-22 neuron^[8]	500 nM; 16 h
NCI-H508^[9]	0.1-10 μM; 48 h
LoVo^[9]	0.1-10 μM; 48 h
LS513^[9]	0.1-10 μM; 48 h
SW480^[9]	0.1-10 μM; 48 h
SW620^[9]	0.1-10 μM; 48 h
SW1116^[9]	0.1-10 μM; 48 h
DLD-1^[9]	0.1-10 μM; 48 h
Caco-2^[9]	0.1-10 μM; 48 h
SW837^[10]	0-40 μM; 24 h
Pfa1^[11]	0.1-5 μM; 48 h
HT-1080^[12]	0.1-5 μM; 48 h
MAD-MB-231^[13]	0-100 μM; 72 h; IC₅₀: 9.55 μM
HCC1937^[13]	0-100 μM; 72 h; IC₅₀: 11.58 μM

Ferroptosis-insensitive Cell Lines

Ferroptosis-insensitive Cells	Test Conditions
HCT116^[11]	0-40 μM; 24 h
SW48^[11]	0-40 μM; 24 h
HepG2^[5]	0-20 μM; 24 h
MDA-MB-436^[13]	0.1-25 μM; 24 h
HT-29^[13]	0.1-25 μM; 24 h
U-373^[13]	0.1-25 μM; 24 h

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	Normal endometrial stromal cells (NESCs) and endometrial stromal cells (EESCs)
Concentration:	0, 0.5, 0.8, 1, 1.5, 2, 2.5, 5, 10 μM
Incubation Time:	24 hours
Result:	Induced cell detachment and overt death in EESCs, but not NESCs.

Apoptosis Analysis^[1]

Cell Line:	EESCs infected with adenovirus expressing FPN cDNA (co-incubation for 24 hr)
Concentration:	0, 0.5, 1.5, 2.5, 5 and 2.5 μM
Incubation Time:	24 hours
Result:	Induced ferroptosis by decreasing the levels of total ROS and lipid ROS. And reversed by the overexpression of FPN in adenovirus-infected cells.

体内実験

Erastin can be used in animal modeling to construct ferroptosis induction model.

Erastin (40 mg/kg; i.p.; once every 3 days for 2 weeks) suppresses endometriotic implants in the mouse endometriosis model, indicating Erastin regresses ectopic lesions by trigging ferroptosis^[1].
Erastin (10 mg/kg, 30 mg/kg; i.p.; once daily for 4 weeks) suppresses HT-29 xenograft growth in SCID mice, with more potent efficacy under 30 mg/kg treatment^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Mouse model of endometriosis^[1]
Dosage:	40 mg/kg
Administration:	Intraperitoneal injection; once every 3 days for 2 weeks
Result:	Showed little impact on body weight of mice and hair of mice displayed neat and glossy. Reduced the volume of ectopic lesions.

分子量

547.04

分子式

C₃₀H₃₁ClN₄O₄

CAS 番号

571203-78-6

Appearance

Solid

Color

White to off-white

SMILES

O=C1N(C2=CC=CC=C2OCC)C(C(N3CCN(C(COC4=CC=C(Cl)C=C4)=O)CC3)C)=NC5=C1C=CC=C5

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

Powder	-20°C	3 years
	4°C	2 years
* The compound is unstable in solutions, freshly prepared is recommended.

溶剤 & 溶解度

体外:

DMSO : 12.5 mg/mL (22.85 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.8280 mL	9.1401 mL	18.2802 mL
5 mM	0.3656 mL	1.8280 mL	3.6560 mL
10 mM	0.1828 mL	0.9140 mL	1.8280 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 1.25 mg/mL (2.29 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL Corn oil, and mix evenly.
Protocol 2

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1 mg/mL (1.83 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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mg/kg

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Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

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Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*The compound is unstable in solutions, freshly prepared is recommended.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

純度とドキュメンテーション

純度: 99.91%

Select Batch:

データシート (288 KB) SDS (396 KB)

COA (250 KB) HNMR (296 KB) RP-HPLC (224 KB) MS (175 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Li Y, et al. Erastin induces ferroptosis via ferroportin-mediated iron accumulation in endometriosis. Hum Reprod. 2021 Mar 18;36(4):951-964. [Content Brief]

[2]. Huo H, et al. Erastin Disrupts Mitochondrial Permeability Transition Pore (mPTP) and Induces Apoptotic Death of Colorectal Cancer Cells. PLoS One. 2016 May 12;11(5):e0154605. [Content Brief]

[3]. Xie Y, et al. Ferroptosis: process and function. Cell Death Differ. 2016 Mar;23(3):369-79. [Content Brief]

[4]. Gai C, et al. MT1DP loaded by folate-modified liposomes sensitizes erastin-induced ferroptosis via regulating miR-365a-3p/NRF2 axis in non-small cell lung cancer cells. Cell Death Dis. 2020 Sep 14;11(9):751. [Content Brief]

[5]. Yang Y, et al. Piperlongumine Inhibits Thioredoxin Reductase 1 by Targeting Selenocysteine Residues and Sensitizes Cancer Cells to Erastin. Antioxidants (Basel). 2022 Apr 4;11(4):710. [Content Brief]

[6]. Liu N, et al., Activation of the reverse transsulfuration pathway through NRF2/CBS confers erastin-induced ferroptosis resistance. Br J Cancer. 2020 Jan;122(2):279-292. [Content Brief]

[7]. Romani P, et al., Mitochondrial fission links ECM mechanotransduction to metabolic redox homeostasis and metastatic chemotherapy resistance. Nat Cell Biol. 2022 Feb;24(2):168-180. [Content Brief]

[8]. Chen T, et al., Mitochondrial transplantation rescues neuronal cells from ferroptosis. Free Radic Biol Med. 2023 Nov 1;208:62-72. [Content Brief]

[9]. Zhu JF, et al., Ibrutinib facilitates the sensitivity of colorectal cancer cells to ferroptosis through BTK/NRF2 pathway. Cell Death Dis. 2023 Feb 23;14(2):151. [Content Brief]

[10]. Xie Y, et al., The Tumor Suppressor p53 Limits Ferroptosis by Blocking DPP4 Activity. Cell Rep. 2017 Aug 15;20(7):1692-1704. [Content Brief]

[11]. Doll S, et al., FSP1 is a glutathione-independent ferroptosis suppressor. Nature. 2019 Nov;575(7784):693-698. [Content Brief]

[12]. Li P, et al., Inhibition of cannabinoid receptor type 1 sensitizes triple-negative breast cancer cells to ferroptosis via regulating fatty acid metabolism. Cell Death Dis. 2022 Sep 21;13(9):808. [Content Brief]

[13]. Zheng J, et al., Sorafenib fails to trigger ferroptosis across a wide range of cancer cell lines. Cell Death Dis. 2021 Jul 13;12(7):698. [Content Brief]

[14]. Wang J, et al. Tumor suppressor BAP1 suppresses disulfidptosis through the regulation of SLC7A11 and NADPH levels[J]. Oncogenesis, 2024, 13(1): 31. [Content Brief]

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.8280 mL	9.1401 mL	18.2802 mL	45.7005 mL
	5 mM	0.3656 mL	1.8280 mL	3.6560 mL	9.1401 mL
	10 mM	0.1828 mL	0.9140 mL	1.8280 mL	4.5700 mL
	15 mM	0.1219 mL	0.6093 mL	1.2187 mL	3.0467 mL
	20 mM	0.0914 mL	0.4570 mL	0.9140 mL	2.2850 mL

Erastin Related Classifications

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量		濃度		体積		分子量 *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）	×	体積 _（開始）	=	濃度 _（終了）	×	体積 _（終了）
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Erastin571203-78-6VDACFerroptosisDisulfidptosisVoltage-dependent anion channel Inhibitorinhibitorinhibit

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Erastin (Synonyms: エラスチン)

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Erastin (Synonyms: エラスチン)

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Other Forms of Erastin:

Erastin 関連抗体

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生物活性

純度とドキュメンテーション

参考文献

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Erastin 関連抗体

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Erastin Related Classifications

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

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