L-NMMA acetate (Synonyms: Tilarginine acetate; Methylarginine acetate)

Name: L-NMMA acetate
Brand: MedChemExpress
SKU: HY-18732A
Rating: 5.0 (11 reviews)

製品番号: HY-18732A 純度: 99.0%: Data Sheet SDS COA 取扱説明書
FAQs
Technical Support

L-NMMA (Tilarginine) acetate is a non-selective and competitive inhibitor of nitric oxide synthase. L-NMMA acetate inhibits three subtypes, namely nNOS, eNOS, and iNOS, and reduces NO production. L-NMMA acetate alleviates mechanical allodynia, thermal hyperalgesia, and choroidal fibrosis. L-NMMA acetate is applicable to research related to nociception, bone cancer pain, and myopia.

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CAS 番号 : 53308-83-1

容量	価格（税別）	在庫状況	数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	USD 55	在庫あり	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	USD 55	在庫あり	Estimated Time of Arrival: December 31
Solid
1 mg	$25	在庫あり	Estimated Time of Arrival: December 31
5 mg	$50	在庫あり	Estimated Time of Arrival: December 31
10 mg	$70	在庫あり	Estimated Time of Arrival: December 31
25 mg	$120	在庫あり	Estimated Time of Arrival: December 31
50 mg	$170	在庫あり	Estimated Time of Arrival: December 31
100 mg	$248	在庫あり	Estimated Time of Arrival: December 31
200 mg		お問い合わせ
500 mg		お問い合わせ

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* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

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Based on 11 publication(s) in Google Scholar

Other Forms of L-NMMA acetate:

L-NMMA 在庫あり
L-NMMA citrate お問い合わせ
L-NMMA hydrochloride お問い合わせ
L-NMMA acetate (Standard) お問い合わせ

顧客検証

Bio/Physico-chemical Assay

Cell Proliferation/Viability Assay

: L-NMMA acetate purchased from MedChemExpress. Usage Cited in: Nat Immunol. 2023 Jan;24(1):162-173. [Abstract]; L-NMMA acetate (200 μM; 48 h) led to elevating ammonia and inhibiting CD8⁺ T_M cell induction, in parallel with the inhibition of NO production.

: L-NMMA acetate purchased from MedChemExpress. Usage Cited in: Nat Immunol. 2023 Jan;24(1):162-173. [Abstract]; L-NMMA acetate (100 μM; 48 h) led to the accumulation of m + 1 arginine and a slowed nitrogen flow in CD8⁺ T_M cells.

: L-NMMA acetate purchased from MedChemExpress. Usage Cited in: Nat Immunol. 2023 Jan;24(1):162-173. [Abstract]; L-NMMA acetate (100 μM; 48 h) blocked formation of CD8⁺ T_M cells.

: L-NMMA acetate purchased from MedChemExpress. Usage Cited in: Nat Immunol. 2023 Jan;24(1):162-173. [Abstract]; CD8⁺ T_M cells were treated with L-NMMA acetate (100 μM; 48 h) and the level of urea was analyzed.

: L-NMMA acetate purchased from MedChemExpress. Usage Cited in: Nat Immunol. 2023 Jan;24(1):162-173. [Abstract]; IL-15-derived CD8⁺ T_M cells were treated with L-NMMA acetate (100 μM; 48 h), then were cultured with [U4] ¹⁵N-arginine for 6 hours and LC-MS/MS analysis was performed for m + 2, m + 3 citrulline, m + 2 ornithine and m + 2 urea.

NO Synthase アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

nNOS iNOS eNOS

生物活性
純度とドキュメンテーション
参考文献
カスタマーレビュー

製品説明

IC₅₀ & Target

eNOS

iNOS

nNOS

Cellular Effect

Cell Line	Type	Value	Description	References
RAW264.7	IC₅₀	17 3 Compound: L-NMMA	Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production pre-incubated for 2 hrs before LPS stimulation for 24 hrs by nitrate/nitrite assay Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production pre-incubated for 2 hrs before LPS stimulation for 24 hrs by nitrate/nitrite assay	[PMID: 31095383]
RAW264.7	CC₅₀	214.41 3 Compound: L-NMMA	Cytotoxicity against mouse RAW264.7 cells assessed as effect on cell viability measured after 12 hrs by MTT assay Cytotoxicity against mouse RAW264.7 cells assessed as effect on cell viability measured after 12 hrs by MTT assay	[PMID: 35503996]
RAW264.7	IC₅₀	20.34 3 Compound: L-NMMA	Antiinflammatory activity in mouse RAW264.7 assessed as inhibition of LPS-induced NO production preincubated for 12 hrs followed by LPS stimulation and measured after 6 hrs by Griess reagent based assay Antiinflammatory activity in mouse RAW264.7 assessed as inhibition of LPS-induced NO production preincubated for 12 hrs followed by LPS stimulation and measured after 6 hrs by Griess reagent based assay	[PMID: 35503996]
RAW264.7	IC₅₀	20.34 3 Compound: L-NMMA	Antiinflammatory activity in mouse RAW264.7 assessed as inhibition of LPS-induced NO production preincubated for 12 hrs followed by LPS stimulation and measured after 6 hrs by Griess reagent based assay Antiinflammatory activity in mouse RAW264.7 assessed as inhibition of LPS-induced NO production preincubated for 12 hrs followed by LPS stimulation and measured after 6 hrs by Griess reagent based assay	[PMID: 35503996]
RAW264.7	IC₅₀	65.5 3 Compound: L-NMMA monoacetate	Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production	[PMID: 31836445]
RAW264.7	CC₅₀	214.41 3 Compound: L-NMMA	Cytotoxicity against mouse RAW264.7 cells assessed as effect on cell viability measured after 12 hrs by MTT assay Cytotoxicity against mouse RAW264.7 cells assessed as effect on cell viability measured after 12 hrs by MTT assay	[PMID: 35503996]
RAW264.7	IC₅₀	39.9 3 Compound: L-NMMA	Inhibition of LPS-induced NO production in mouse RAW264.7 cells after 18 hrs by Griess assay Inhibition of LPS-induced NO production in mouse RAW264.7 cells after 18 hrs by Griess assay	[PMID: 28654256]
RAW264.7	IC₅₀	17 3 Compound: L-NMMA	Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production pre-incubated for 2 hrs before LPS stimulation for 24 hrs by nitrate/nitrite assay Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production pre-incubated for 2 hrs before LPS stimulation for 24 hrs by nitrate/nitrite assay	[PMID: 31095383]
RAW264.7	IC₅₀	47.2 3 Compound: L-NMMA	Inhibition of LPS-induced NO production in mouse RAW264.7 cells incubated for 18 hrs by Griess reagent based assay Inhibition of LPS-induced NO production in mouse RAW264.7 cells incubated for 18 hrs by Griess reagent based assay	[PMID: 33325237]
RAW264.7	IC₅₀	39.9 3 Compound: L-NMMA	Inhibition of LPS-induced NO production in mouse RAW264.7 cells after 18 hrs by Griess assay Inhibition of LPS-induced NO production in mouse RAW264.7 cells after 18 hrs by Griess assay	[PMID: 28654256]
RAW264.7	IC₅₀	78.3 3 Compound: L-NMMA	Inhibition of NO production in mouse RAW264.7 cells incubated for 24 hrs by Griess reagent based analysis Inhibition of NO production in mouse RAW264.7 cells incubated for 24 hrs by Griess reagent based analysis	[PMID: 34890996]
RAW264.7	IC₅₀	47.2 3 Compound: L-NMMA	Inhibition of LPS-induced NO production in mouse RAW264.7 cells incubated for 18 hrs by Griess reagent based assay Inhibition of LPS-induced NO production in mouse RAW264.7 cells incubated for 18 hrs by Griess reagent based assay	[PMID: 33325237]
RAW264.7	CC₅₀	214.41 3 Compound: L-NMMA	Cytotoxicity against mouse RAW264.7 cells assessed as effect on cell viability measured after 12 hrs by MTT assay Cytotoxicity against mouse RAW264.7 cells assessed as effect on cell viability measured after 12 hrs by MTT assay	[PMID: 35503996]
RAW264.7	IC₅₀	78.3 3 Compound: L-NMMA	Inhibition of NO production in mouse RAW264.7 cells incubated for 24 hrs by Griess reagent based analysis Inhibition of NO production in mouse RAW264.7 cells incubated for 24 hrs by Griess reagent based analysis	[PMID: 34890996]
RAW264.7	IC₅₀	17 3 Compound: L-NMMA	Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production pre-incubated for 2 hrs before LPS stimulation for 24 hrs by nitrate/nitrite assay Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production pre-incubated for 2 hrs before LPS stimulation for 24 hrs by nitrate/nitrite assay	[PMID: 31095383]
RAW264.7	IC₅₀	20.34 3 Compound: L-NMMA	Antiinflammatory activity in mouse RAW264.7 assessed as inhibition of LPS-induced NO production preincubated for 12 hrs followed by LPS stimulation and measured after 6 hrs by Griess reagent based assay Antiinflammatory activity in mouse RAW264.7 assessed as inhibition of LPS-induced NO production preincubated for 12 hrs followed by LPS stimulation and measured after 6 hrs by Griess reagent based assay	[PMID: 35503996]
RAW264.7	IC₅₀	39.9 3 Compound: L-NMMA	Inhibition of LPS-induced NO production in mouse RAW264.7 cells after 18 hrs by Griess assay Inhibition of LPS-induced NO production in mouse RAW264.7 cells after 18 hrs by Griess assay	[PMID: 28654256]
RAW264.7	IC₅₀	47.2 3 Compound: L-NMMA	Inhibition of LPS-induced NO production in mouse RAW264.7 cells incubated for 18 hrs by Griess reagent based assay Inhibition of LPS-induced NO production in mouse RAW264.7 cells incubated for 18 hrs by Griess reagent based assay	[PMID: 33325237]
RAW264.7	IC₅₀	65.5 3 Compound: L-NMMA monoacetate	Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production	[PMID: 31836445]
RAW264.7	IC₅₀	78.3 3 Compound: L-NMMA	Inhibition of NO production in mouse RAW264.7 cells incubated for 24 hrs by Griess reagent based analysis Inhibition of NO production in mouse RAW264.7 cells incubated for 24 hrs by Griess reagent based analysis	[PMID: 34890996]

体内実験

L-NMMA (50 µg; i.t.; single administration) acetate significantly alleviates bone cancer-induced mechanical allodynia and thermal hyperalgesia in mice at 2 and 12 h post-treatment^[1].
L-NMMA (20 nmol; intravitreal injection; once every other day; 2 to 4 weeks) acetate alleviates choroidal fibrosis and delays myopia progression by inhibiting the NO signaling pathway in lens-induced myopic guinea pigs^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C3H/HeJ (male, 4-6 weeks old, 20-22 g, intramedullary inoculation of NCTC 2472 osteosarcoma cells)^[1]
Dosage:	50 µg
Administration:	i.t.; single administration
Result:	Increased paw withdrawal mechanical threshold to 0.90 g at 2 h and 0.63 g at 12 h compared to 0.40 g in vehicle-treated tumor mice. Increased paw withdrawal thermal latency to 16.2 sec at 2 h and 12.7 sec at 12 h compared to 10.1 sec in vehicle-treated tumor mice. Lost analgesic effect at 24 h post-administration.

Animal Model:	British short-haired tricolor guinea pigs (2-week-old, 110-130 g, lens-induced myopia model)^[2]
Dosage:	20 nmol
Administration:	intravitreal injection; once every other day; 2 and 4 weeks
Result:	Increased refraction significantly after 2 and 4 weeks compared to the lens-induced myopia group. Reduced the D-value of axial length between the myopic eye and control eye significantly after 2 and 4 weeks compared to the lens-induced myopia group. Increased choroidal thickness to 80.62 μm at 4 weeks compared to 63.54 μm in the lens-induced myopia group. Reduced degradation of choroidal pigment particles and narrowed particle gaps compared to the lens-induced myopia group. Produced denser, more orderly choroidal tissue structure compared to the lens-induced myopia group. Reduced choroidal fibrosis compared to the lens-induced myopia group. Lowered gene expression levels of NOS1, NOS3, TGF-β1, COL I, and α-SMA significantly after 2 and 4 weeks compared to the lens-induced myopia group. Lowered protein levels of NOS1, NOS3, TGF-β1, COL I, and α-SMA significantly after 2 and 4 weeks compared to the lens-induced myopia group. Reduced expression of COL I, α-SMA, NOS1, NOS3, and TGF-β1 in choroidal tissues compared to the lens-induced myopia group.

分子量

248.28

分子式

C₉H₂₀N₄O₄

CAS 番号

53308-83-1

Appearance

Solid

Color

White to off-white

SMILES

N[C@@H](CCCNC(NC)=N)C(O)=O.O=C(C)O

Structure Classification

Others

Initial Source

Endogenous metabolite

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶剤 & 溶解度

体外:

H₂O : ≥ 50 mg/mL (201.39 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.0277 mL	20.1386 mL	40.2771 mL
5 mM	0.8055 mL	4.0277 mL	8.0554 mL
10 mM	0.4028 mL	2.0139 mL	4.0277 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

体内:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (402.77 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

純度とドキュメンテーション

純度: 99.0%

Select Batch:

データシート (289 KB) SDS (393 KB)

COA (246 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Yang Y, et al. Role of nitric oxide synthase in the development of bone cancer pain and effect of L-NMMA. Mol Med Rep. 2016;13(2):1220-1226.

[2]. Li T, et al. Suppressive effect of nitric oxide synthase (NOS) inhibitor L-NMMA acetate on choroidal fibrosis in experimental myopic guinea pigs through the nitric oxide signaling pathway. Eur J Pharmacol. 2023;960:176111.

[3]. Babbedge RC, et al. Anti-nociceptive activity of nitric oxide synthase inhibitors in the mouse: dissociation between the effect of L-NAME and L-NMMA. J Pharm Pharmacol. 1993;45(1):77-79.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	4.0277 mL	20.1386 mL	40.2771 mL	100.6928 mL
	5 mM	0.8055 mL	4.0277 mL	8.0554 mL	20.1386 mL
	10 mM	0.4028 mL	2.0139 mL	4.0277 mL	10.0693 mL
	15 mM	0.2685 mL	1.3426 mL	2.6851 mL	6.7129 mL
	20 mM	0.2014 mL	1.0069 mL	2.0139 mL	5.0346 mL
	25 mM	0.1611 mL	0.8055 mL	1.6111 mL	4.0277 mL
	30 mM	0.1343 mL	0.6713 mL	1.3426 mL	3.3564 mL
	40 mM	0.1007 mL	0.5035 mL	1.0069 mL	2.5173 mL
	50 mM	0.0806 mL	0.4028 mL	0.8055 mL	2.0139 mL
	60 mM	0.0671 mL	0.3356 mL	0.6713 mL	1.6782 mL
	80 mM	0.0503 mL	0.2517 mL	0.5035 mL	1.2587 mL
	100 mM	0.0403 mL	0.2014 mL	0.4028 mL	1.0069 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

L-NMMA acetate Related Classifications

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量		濃度		体積		分子量 *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）	×	体積 _（開始）	=	濃度 _（終了）	×	体積 _（終了）
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

L-NMMA53308-83-1TilarginineMethylarginineNO SynthaseNitric oxide synthasesNOSInhibitorinhibitorinhibit

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L-NMMA acetate (Synonyms: Tilarginine acetate; Methylarginine acetate)

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Complete Stock Solution Preparation Table

L-NMMA acetate Related Classifications

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The molarity calculator equation

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L-NMMA acetate (Synonyms: Tilarginine acetate; Methylarginine acetate)

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Other Forms of L-NMMA acetate:

L-NMMA acetate 関連抗体

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おすすめ

•関連スクリーニングライブラリ:

•関連小分子:

•関連タンパク質:

NO Synthase アイソフォーム固有の製品をすべて表示:

生物活性

純度とドキュメンテーション

参考文献

カスタマーレビュー

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

L-NMMA acetate Related Classifications

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

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