L-NMMA citrate
Based on 11 publication(s) in Google Scholar
L-NMMA (Tilarginine) citrate is a non-selective and competitive inhibitor of nitric oxide synthase. L-NMMA citrate inhibits three subtypes, namely nNOS, eNOS, and iNOS, and reduces NO production. L-NMMA citrate alleviates mechanical allodynia, thermal hyperalgesia, and choroidal fibrosis. L-NMMA citrate is applicable to research related to nociception, bone cancer pain, and myopia.
For research use only. We do not sell to patients.
- CAS No.: 209913-88-2
- Formula: C13H24N4O9
- Molecular Weight:380.35
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Publications Citing Use of MedChemExpress (MCE) L-NMMA citrate
More- Cell Metab. 2025 Jan 7;37(1):291-304.e9. [Abstract]
- Nat Immunol. 2023 Jan;24(1):162-173. [Abstract]
- Nat Commun. 2021 May 10;12(1):2587. [Abstract]
- Cell Mol Gastroenterol Hepatol. 2025 Feb 7:101474. [Abstract]
- Cell Mol Gastroenterol Hepatol. 2021;11(3):683-696. [Abstract]
- EMBO Rep. 2025 Oct 20. [Abstract]
- Life Sci. 2025 Jan 24:123415. [Abstract]
- Front Cell Dev Biol. 2021 Dec 23;9:741911. [Abstract]
- FASEB J. 2025 Sep 30;39(18):e71057. [Abstract]
- Eur J Immunol. 2020 Jun;50(6):795-808. [Abstract]
- Theriogenology. 2025 May 29:245:117517. [Abstract]
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Cell Proliferation/Viability Assay
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Biological Activity
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Cell Line
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Type | Value | Description | References |
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| RAW264.7 | IC50 |
22.7 μM
Compound: L-NMMA
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Inhibition of NO production in LPS-stimulated mouse RAW264.7 cells preincubated for 15 mins followed by LPS stimulation and measured after 20 hrs by Griess reagent based assay
Inhibition of NO production in LPS-stimulated mouse RAW264.7 cells preincubated for 15 mins followed by LPS stimulation and measured after 20 hrs by Griess reagent based assay
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[PMID: 36746775] |
| RAW264.7 | IC50 |
25.1 μM
Compound: L-NMMA
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Antiinflammatory activity against LPS-induced NO production in mouse RAW264.7 cells assessed as reduction in nitrite level preincubated for 15 mins prior to LPS treatment by Griess method
Antiinflammatory activity against LPS-induced NO production in mouse RAW264.7 cells assessed as reduction in nitrite level preincubated for 15 mins prior to LPS treatment by Griess method
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[PMID: 22850207] |
| RAW264.7 | IC50 |
25.1 μM
Compound: L-NMMA
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Cancer chemopreventive activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production treated 30 mins before LPS challenge measured after 24 hrs by Griess reagent assay
Cancer chemopreventive activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production treated 30 mins before LPS challenge measured after 24 hrs by Griess reagent assay
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[PMID: 23316950] |
| RAW264.7 | IC50 |
25.1 μM
Compound: L-NMMA
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Cytotoxicity against mouse RAW264.7 cells assessed as cell survival by SRB assay
Cytotoxicity against mouse RAW264.7 cells assessed as cell survival by SRB assay
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[PMID: 23316950] |
| RAW264.7 | IC50 |
25.1 μM
Compound: N-monomethyl-L-arginine citrate
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Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide overproduction pretreated with LPS for 24 hrs followed by overnight incubation with compound by Griess assay
Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide overproduction pretreated with LPS for 24 hrs followed by overnight incubation with compound by Griess assay
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[PMID: 31411887] |
| RAW264.7 | IC50 |
25.49 μM
Compound: R9C1Table 2
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Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production incubated 30 mins prior to LPS challenge
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production incubated 30 mins prior to LPS challenge
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[PMID: 20685125] |
| RAW264.7 | IC50 |
32 μM
Compound: L-NMMA
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Inhibition of iNOS-mediated NO production in mouse RAW264.7 cells by Griess assay
Inhibition of iNOS-mediated NO production in mouse RAW264.7 cells by Griess assay
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[PMID: 23994869] |
L-NMMA (20 nmol; intravitreal injection; once every other day; 2 to 4 weeks) citrate alleviates choroidal fibrosis and delays myopia progression by inhibiting the NO signaling pathway in lens-induced myopic guinea pigs[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C3H/HeJ (male, 4-6 weeks old, 20-22 g, intramedullary inoculation of NCTC 2472 osteosarcoma cells)[1]
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Dosage:50 µg
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Administration:i.t.; single administration
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Result:Increased paw withdrawal mechanical threshold to 0.90 g at 2 h and 0.63 g at 12 h compared to 0.40 g in vehicle-treated tumor mice.
Increased paw withdrawal thermal latency to 16.2 sec at 2 h and 12.7 sec at 12 h compared to 10.1 sec in vehicle-treated tumor mice.
Lost analgesic effect at 24 h post-administration.
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Animal Model:British short-haired tricolor guinea pigs (2-week-old, 110-130 g, lens-induced myopia model)[2]
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Dosage:20 nmol
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Administration:intravitreal injection; once every other day; 2 and 4 weeks
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Result:Increased refraction significantly after 2 and 4 weeks compared to the lens-induced myopia group.
Reduced the D-value of axial length between the myopic eye and control eye significantly after 2 and 4 weeks compared to the lens-induced myopia group.
Increased choroidal thickness to 80.62 μm at 4 weeks compared to 63.54 μm in the lens-induced myopia group.
Reduced degradation of choroidal pigment particles and narrowed particle gaps compared to the lens-induced myopia group.
Produced denser, more orderly choroidal tissue structure compared to the lens-induced myopia group.
Reduced choroidal fibrosis compared to the lens-induced myopia group.
Lowered gene expression levels of NOS1, NOS3, TGF-β1, COL I, and α-SMA significantly after 2 and 4 weeks compared to the lens-induced myopia group.
Lowered protein levels of NOS1, NOS3, TGF-β1, COL I, and α-SMA significantly after 2 and 4 weeks compared to the lens-induced myopia group.
Reduced expression of COL I, α-SMA, NOS1, NOS3, and TGF-β1 in choroidal tissues compared to the lens-induced myopia group.
Chemical Information
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CAS No. 209913-88-2
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Molecular Weight 380.35
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Formula C13H24N4O9
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SMILES
N[C@H](C(O)=O)CCCNC(NC)=N.O=C(O)CC(O)(CC(O)=O)C(O)=O
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Synonyms
Methylarginine citrate; Tilarginine citrate
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (11)
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Journal Impact Factor
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Most Recent
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Cell Metab
Lighting up arginine metabolism reveals its functional diversity in physiology and pathology. [Abstract]2025 Jan 7;37(1):291-304.e9. PMID: 39413790 -
Nat Immunol
Ammonia detoxification promotes CD8+ T cell memory development by urea and citrulline cycles. [Abstract]2023 Jan;24(1):162-173. PMID: 36471170
L-NMMA citrate purchased from MedChemExpress. Usage Cited in: Nat Immunol. 2023 Jan;24(1):162-173. [Abstract]
L-NMMA acetate (200 μM; 48 h) led to elevating ammonia and inhibiting CD8+ TM cell induction, in parallel with the inhibition of NO production.
L-NMMA citrate purchased from MedChemExpress. Usage Cited in: Nat Immunol. 2023 Jan;24(1):162-173. [Abstract]
L-NMMA acetate (100 μM; 48 h) led to the accumulation of m + 1 arginine and a slowed nitrogen flow in CD8+ TM cells.
L-NMMA citrate purchased from MedChemExpress. Usage Cited in: Nat Immunol. 2023 Jan;24(1):162-173. [Abstract]
L-NMMA acetate (100 μM; 48 h) blocked formation of CD8+ TM cells.
L-NMMA citrate purchased from MedChemExpress. Usage Cited in: Nat Immunol. 2023 Jan;24(1):162-173. [Abstract]
CD8+ TM cells were treated with L-NMMA acetate (100 μM; 48 h) and the level of urea was analyzed.
L-NMMA citrate purchased from MedChemExpress. Usage Cited in: Nat Immunol. 2023 Jan;24(1):162-173. [Abstract]
IL-15-derived CD8+ TM cells were treated with L-NMMA acetate (100 μM; 48 h), then were cultured with [U4] 15N-arginine for 6 hours and LC-MS/MS analysis was performed for m + 2, m + 3 citrulline, m + 2 ornithine and m + 2 urea.
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Nat Commun
An infection-induced RhoB-Beclin 1-Hsp90 complex enhances clearance of uropathogenic Escherichia coli. [Abstract]2021 May 10;12(1):2587. PMID: 33972537 -
Cell Mol Gastroenterol Hepatol
2025 Feb 7:101474. PMID: 39923847 -
Cell Mol Gastroenterol Hepatol
2021;11(3):683-696. PMID: 33075564 -
EMBO Rep
Tudor-based proteomic strategy pan-specifically enriches and identifies protein arginine methylation. [Abstract]2025 Oct 20. PMID: 41116072 -
Life Sci
Identification and regulation of a novel leptin receptor-linked enhancer during zebrafish ventricle regeneration. [Abstract]2025 Jan 24:123415. PMID: 39864617 -
Front Cell Dev Biol
mTOR Signaling Regulates the Development and Therapeutic Efficacy of PMN-MDSCs in Acute GVHD. [Abstract]2021 Dec 23;9:741911. PMID: 35004668 -
FASEB J
Spatial Metabolism of Primary Limited Cutaneous Amyloidosis Based on Mass Spectrometry Imaging. [Abstract]2025 Sep 30;39(18):e71057. PMID: 40955599 -
Eur J Immunol
Fibroblast transdifferentiation promotes conversion of M1 macrophages and replenishment of cardiac resident macrophages following cardiac injury in mice. [Abstract]2020 Jun;50(6):795-808. PMID: 32068249 -
Theriogenology
2025 May 29:245:117517. PMID: 40460471
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
L-NMMA citrate Related Classifications
HY-18732B Related Classifications
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