2. Immunology/Inflammation Metabolic Enzyme/Protease
  3. NO Synthase Endogenous Metabolite
  4. L-NMMA hydrochloride

L-NMMA hydrochloride  (Synonyms: Tilarginine hydrochloride; Methylarginine hydrochloride)

Cat. No.: HY-18732C
Handling Instructions Technical Support

L-NMMA (Tilarginine) hydrochloride is a non-selective and competitive inhibitor of nitric oxide synthase. L-NMMA hydrochloride inhibits three subtypes, namely nNOS, eNOS, and iNOS, and reduces NO production. L-NMMA hydrochloride alleviates mechanical allodynia, thermal hyperalgesia, and choroidal fibrosis. L-NMMA hydrochloride is applicable to research related to nociception, bone cancer pain, and myopia.

For research use only. We do not sell to patients.

CAS No. : 156706-47-7

Size Stock
50 mg   Get quote  
100 mg   Get quote  
250 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Other In-stock Forms of L-NMMA hydrochloride:

Other Forms of L-NMMA hydrochloride:

L-NMMA hydrochloride Related Antibodies

Top Publications Citing Use of Products

    L-NMMA hydrochloride purchased from MedChemExpress. Usage Cited in: Nat Immunol. 2023 Jan;24(1):162-173.  [Abstract]

    L-NMMA acetate (200 μM; 48 h) led to elevating ammonia and inhibiting CD8+ TM cell induction, in parallel with the inhibition of NO production.

    L-NMMA hydrochloride purchased from MedChemExpress. Usage Cited in: Nat Immunol. 2023 Jan;24(1):162-173.  [Abstract]

    L-NMMA acetate (100 μM; 48 h) led to the accumulation of m + 1 arginine and a slowed nitrogen flow in CD8+ TM cells.

    L-NMMA hydrochloride purchased from MedChemExpress. Usage Cited in: Nat Immunol. 2023 Jan;24(1):162-173.  [Abstract]

    L-NMMA acetate (100 μM; 48 h) blocked formation of CD8+ TM cells.

    L-NMMA hydrochloride purchased from MedChemExpress. Usage Cited in: Nat Immunol. 2023 Jan;24(1):162-173.  [Abstract]

    CD8+ TM cells were treated with L-NMMA acetate (100 μM; 48 h) and the level of urea was analyzed.

    L-NMMA hydrochloride purchased from MedChemExpress. Usage Cited in: Nat Immunol. 2023 Jan;24(1):162-173.  [Abstract]

    IL-15-derived CD8+ TM cells were treated with L-NMMA acetate (100 μM; 48 h), then were cultured with [U4] 15N-arginine for 6 hours and LC-MS/MS analysis was performed for m + 2, m + 3 citrulline, m + 2 ornithine and m + 2 urea.

    View All NO Synthase Isoform Specific Products:

    View All Isoforms
    nNOS iNOS eNOS

    View All Endogenous Metabolite Isoform Specific Products:

    View All Isoforms
    Human Endogenous Metabolite Microbial Metabolite

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    L-NMMA (Tilarginine) hydrochloride is a non-selective and competitive inhibitor of nitric oxide synthase. L-NMMA hydrochloride inhibits three subtypes, namely nNOS, eNOS, and iNOS, and reduces NO production. L-NMMA hydrochloride alleviates mechanical allodynia, thermal hyperalgesia, and choroidal fibrosis. L-NMMA hydrochloride is applicable to research related to nociception, bone cancer pain, and myopia[1][2][3].

    In Vivo

    L-NMMA (50 µg; i.t.; single administration) hydrochloride significantly alleviates bone cancer-induced mechanical allodynia and thermal hyperalgesia in mice at 2 and 12 h post-treatment[1].
    L-NMMA (20 nmol; intravitreal injection; once every other day; 2 to 4 weeks) hydrochloride alleviates choroidal fibrosis and delays myopia progression by inhibiting the NO signaling pathway in lens-induced myopic guinea pigs[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: C3H/HeJ (male, 4-6 weeks old, 20-22 g, intramedullary inoculation of NCTC 2472 osteosarcoma cells)[1]
    Dosage: 50 µg
    Administration: i.t.; single administration
    Result: Increased paw withdrawal mechanical threshold to 0.90 g at 2 h and 0.63 g at 12 h compared to 0.40 g in vehicle-treated tumor mice.
    Increased paw withdrawal thermal latency to 16.2 sec at 2 h and 12.7 sec at 12 h compared to 10.1 sec in vehicle-treated tumor mice.
    Lost analgesic effect at 24 h post-administration.
    Animal Model: British short-haired tricolor guinea pigs (2-week-old, 110-130 g, lens-induced myopia model)[2]
    Dosage: 20 nmol
    Administration: intravitreal injection; once every other day; 2 and 4 weeks
    Result: Increased refraction significantly after 2 and 4 weeks compared to the lens-induced myopia group.
    Reduced the D-value of axial length between the myopic eye and control eye significantly after 2 and 4 weeks compared to the lens-induced myopia group.
    Increased choroidal thickness to 80.62 μm at 4 weeks compared to 63.54 μm in the lens-induced myopia group.
    Reduced degradation of choroidal pigment particles and narrowed particle gaps compared to the lens-induced myopia group.
    Produced denser, more orderly choroidal tissue structure compared to the lens-induced myopia group.
    Reduced choroidal fibrosis compared to the lens-induced myopia group.
    Lowered gene expression levels of NOS1, NOS3, TGF-β1, COL I, and α-SMA significantly after 2 and 4 weeks compared to the lens-induced myopia group.
    Lowered protein levels of NOS1, NOS3, TGF-β1, COL I, and α-SMA significantly after 2 and 4 weeks compared to the lens-induced myopia group.
    Reduced expression of COL I, α-SMA, NOS1, NOS3, and TGF-β1 in choroidal tissues compared to the lens-induced myopia group.
    Molecular Weight

    224.69

    Formula

    C7H17ClN4O2
    CAS No.
    SMILES

    N[C@@H](CCCNC(NC)=N)C(O)=O.Cl
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Please store the product under the recommended conditions in the Certificate of Analysis.
    Purity & Documentation
    References
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    L-NMMA hydrochloride Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass   Concentration   Volume   Molecular Weight *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    L-NMMA156706-47-7TilarginineMethylarginineNO SynthaseEndogenous MetaboliteNitric oxide synthasesNOScompetitiveInhibitorinhibitorinhibit

    Your Recently Viewed Products:

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Requested Quantity *

    Applicant Name *

    		 

    Salutation

    Email Address *

    		 

    Phone Number *

    Department

    		 

    Organization Name *

    City

    State

    Country or Region *

    		      

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product Name:
    L-NMMA hydrochloride
    Cat. No.:
    HY-18732C
    Quantity:
    MCE Japan Authorized Agent:

    Customer Review

    Thank You for Your Feedback!

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Lot #

    Applicant Name *

    		 

    Email Address *

    Phone Number

    		 

    Department *

    Organization Name *

    		 

    Country or Region *

    Remarks

    SAFETY DATA SHEET (SDS)

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.