Melittin

Name: Melittin
Brand: MedChemExpress
SKU: HY-P0233
Rating: 5.0 (16 reviews)

Cat. No.: HY-P0233 Purity: 99.73%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Melittin is a PLA₂ activator, stimulates the activity of the low molecular weight PLA₂, while it does not the increase activity of the high molecular weight PLA₂.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

Custom Peptide Synthesis

CAS No. : 20449-79-0

사이즈	재고	수량
5 mg	해외재고보유	Estimated Time of Arrival: December 31
10 mg	해외재고보유	Estimated Time of Arrival: December 31
25 mg	해외재고보유	Estimated Time of Arrival: December 31
50 mg	해외재고보유	Estimated Time of Arrival: December 31
100 mg	해외재고보유	Estimated Time of Arrival: December 31
200 mg	견적 받기
500 mg	견적 받기

or 대량구매 문의

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 16 publication(s) in Google Scholar

Other Forms of Melittin:

Melittin TFA In-stock
Melittin free acid 견적 받기

16 Publications Citing Use of MCE Melittin

Cell Proliferation/Viability Assay

Cell Imaging/Staining

Flow Cytometry

In Vivo Efficacy Study

: Melittin purchased from MedChemExpress. Usage Cited in: Adv Healthc Mater. 2025 Apr;14(9):e2400543. [Abstract]; Melittin (1 μg/mL) alleviated the acute toxicity of MC complexes for FLS within 1 h.

: Melittin purchased from MedChemExpress. Usage Cited in: Adv Healthc Mater. 2025 Apr;14(9):e2400543. [Abstract]; Melittin (1 μg/mL). Holes in the cell membrane of FLS cultured with MC complexes decreased at 1 h, as observed by SEM.

: Melittin purchased from MedChemExpress. Usage Cited in: Adv Healthc Mater. 2025 Apr;14(9):e2400543. [Abstract]; The apoptosis ratio of FLS cultured with MC complexes at 4 h was increased by FCM. Melittin was used at 1 μg/mL.

: Melittin purchased from MedChemExpress. Usage Cited in: Adv Healthc Mater. 2025 Apr;14(9):e2400543. [Abstract]; Reduced expression of pIKKβ and pp65 in FLS cultured with MC complexes at 24 h was observed by WB with Melittin (1 μg/mL).

: Melittin purchased from MedChemExpress. Usage Cited in: Adv Healthc Mater. 2025 Apr;14(9):e2400543. [Abstract]; The diffuse FITC-MEL in the paw skin after FITC-MC@CSMA-MNPs attachment was observed. Scale bars represented 100 μm. CCMNPs were loaded with FITC-labeled MEL and CS (FITC-MC complexes).

: Melittin purchased from MedChemExpress. Usage Cited in: J Pharm Pharmacol. 2023 Apr 17;75(5):645-654. [Abstract]; Melittin (2.5, 5, 10 μg/mL; 48 h) downregulates the expression of Bcl-2, and increases the levels of the pro-apoptotic molecules Bax and cyt-c, in SW480 cells.

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Phospholipase Isoform Specific Products:

View All Isoforms

Phospholipase A PLD Phospholipase C

Biological Activity
Protocol
순도&문서
References
고객리뷰

제품 설명

Melittin is a PLA₂ activator, stimulates the activity of the low molecular weight PLA₂, while it does not the increase activity of the high molecular weight PLA₂^[1]^[2].

IC₅₀ & Target

PLA₂^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
Caco-2	IC₅₀	4.5 3 Compound: Melittin	Cytotoxicity against human Caco2 cells after 1 hr by MTS/PMS assay Cytotoxicity against human Caco2 cells after 1 hr by MTS/PMS assay	[PMID: 24601601]
Erythrocyte	CC₅₀	0.2 3 Compound: Melittin	Hemolytic activity against human RBC assessed as concentration required to cause 50% hemolysis measured after 1 hr Hemolytic activity against human RBC assessed as concentration required to cause 50% hemolysis measured after 1 hr	[PMID: 33397096]
Erythrocyte	IC₅₀	0.96 3 Compound: Melittin	Hemolytic activity against human RBC after 1 hr Hemolytic activity against human RBC after 1 hr	[PMID: 30387611]
HEK-293T	IC₅₀	2.484 3 Compound: Melittin	Cytotoxicity against HEK293T cells assessed as cell death after 18 to 24 hrs by MTT assay Cytotoxicity against HEK293T cells assessed as cell death after 18 to 24 hrs by MTT assay	[PMID: 30742437]
HeLa	IC₅₀	1.9 3 Compound: Melittin	Cytotoxicity against human HeLa cells after 1 hr by MTS/PMS assay Cytotoxicity against human HeLa cells after 1 hr by MTS/PMS assay	[PMID: 24601601]
HepG2	IC₅₀	2.5 3 Compound: Melittin	Cytotoxicity against human HepG2 cells after 1 hr by MTS/PMS assay Cytotoxicity against human HepG2 cells after 1 hr by MTS/PMS assay	[PMID: 24601601]
HUVEC	IC₅₀	13 3 Compound: 1	Antiangiogenic activity against VEGFA-stimulated capillary differentiation in HUVEC after 18 hrs by matrigel assay Antiangiogenic activity against VEGFA-stimulated capillary differentiation in HUVEC after 18 hrs by matrigel assay	[PMID: 23110475]
HUVEC	IC₅₀	18 3 Compound: 1	Antiangiogenic activity against VEGFA-stimulated cell proliferation in HUVEC after 48 hrs by BrdU incorporation assay Antiangiogenic activity against VEGFA-stimulated cell proliferation in HUVEC after 48 hrs by BrdU incorporation assay	[PMID: 23110475]
IEC-6	IC₅₀	2.2 3 Compound: Melittin	Cytotoxicity against rat IEC-6 cells after 1 hr by MTS/PMS assay Cytotoxicity against rat IEC-6 cells after 1 hr by MTS/PMS assay	[PMID: 24601601]
NIH3T3	IC₅₀	6.4 3 Compound: Melittin	Cytotoxicity against mouse NIH/3T3 cells after 1 hr by MTS/PMS assay Cytotoxicity against mouse NIH/3T3 cells after 1 hr by MTS/PMS assay	[PMID: 24601601]
RAW264.7	IC₅₀	1.3 3 Compound: Melittin	Cytotoxicity against mouse RAW264.7 cells assessed as inhibition of cell growth at pH 6.5 measured after 24 hrs by MTT assay Cytotoxicity against mouse RAW264.7 cells assessed as inhibition of cell growth at pH 6.5 measured after 24 hrs by MTT assay	[PMID: 35294199]
RAW264.7	IC₅₀	1.94 3 Compound: Melittin	Cytotoxicity against mouse RAW264.7 cells assessed as inhibition of cell growth at pH 7.4 measured after 24 hrs by MTT assay Cytotoxicity against mouse RAW264.7 cells assessed as inhibition of cell growth at pH 7.4 measured after 24 hrs by MTT assay	[PMID: 35294199]
RAW264.7	IC₅₀	2.094 3 Compound: Melittin	Cytotoxicity against mouse RAW264.7 cells assessed as cell death after 18 to 24 hrs by MTT assay Cytotoxicity against mouse RAW264.7 cells assessed as cell death after 18 to 24 hrs by MTT assay	[PMID: 30742437]

In Vitro

Melittin, an immunologically related PLA₂ stimulating peptide from bee venom, increases the activity of the high molecular weight enzyme^[1]. Melittin is a cytotoxic peptide from bee venom. Melittin exhibits toxicity against both A2780CR and A2780 cells, with IC₅₀ values of 4.5 and 6.8 μg/mL, respectively. Melittin has natural anti-bacterial, anti-viral, and anti-inflammatory properties. It has also been shown to have diverse anticancer effects in several different cancer cell lines including those of gastric, breast, ovarian, liver, prostate, cervical, and lung origins. The mechanisms by which Melittin, an amphipathic haemolytic peptide, exerts its potential anticancer effects include inhibition of cell proliferation, induction of apoptosis, and direct necrosis. Melittin can also prevent EGF-induced cell invasion through its inhibition of the PI3K/Akt/mTOR signaling pathway, but this is primarily related to breast cancer cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

분자량

2846.46

화학식

C₁₃₁H₂₂₉N₃₉O₃₁

CAS No.

20449-79-0

Appearance

Solid

Color

White to off-white

Sequence

Gly-Ile-Gly-Ala-Val-Leu-Lys-Val-Leu-Thr-Thr-Gly-Leu-Pro-Ala-Leu-Ile-Ser-Trp-Ile-Lys-Arg-Lys-Arg-Gln-Gln-NH2

Sequence Shortening

GIGAVLKVLTTGLPALISWIKRKRQQ-NH2

선적

Room temperature in continental US; may vary elsewhere.

보관

Sealed storage, away from moisture and light

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

용액&용해도

In Vitro:

H₂O : ≥ 50 mg/mL (17.57 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.3513 mL	1.7566 mL	3.5131 mL
5 mM	0.0703 mL	0.3513 mL	0.7026 mL
10 mM	0.0351 mL	0.1757 mL	0.3513 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

몰농도 계산기
농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

순도&문서

Purity: 99.78%

Select Batch:

Data Sheet (280 KB) SDS (480 KB)

COA (242 KB) RP-HPLC (218 KB) LCMS (102 KB) Elemental Analysis Report (100 KB)

Handling Instructions (2659 KB)

References

[1]. Steiner MR, et al. Responses of purified phospholipases A2 to phospholipase A2 activating protein (PLAP) and Melittin. Biochim Biophys Acta. 1993 Feb 10;1166(1):124-30. [Content Brief]

[2]. Alonezi S, et al. Metabolomic Profiling of the Effects of Melittin on Cisplatin Resistant and Cisplatin Sensitive Ovarian Cancer Cells Using Mass Spectrometry and Biolog Microarray Technology. Metabolites. 2016 Oct 13;6(4). pii: E35. [Content Brief]

Cell Assay
^[2]

Melittin is purified from bee venom by reversed phase liquid chromatography and reconstituted in sterile water to form a stock solution of 1 mg/mL before storage at -20 °C until required for analysis. Cell viability is assessed by an Alamar Blue (AB) cell viability reagent. Both A2780 and A2780CR cells are seeded at 1×10⁴ cells/well in 96-well plates and incubated at 37 °C and 5% CO₂ in a humidified atmosphere for 24 h. After this incubation period, the cells are treated with various concentrations of Melittin ranging from 0.5 to 14 µg/mL in 100 μL of medium, and re-incubated at 37 °C and 5% CO₂ for a further 24 h. Triton X at 1% (v/v) and cell culture media are used as positive and negative controls, respectively. After this, AB is added at a final concentration of 10% (v/v) and the resultant mixture is incubated for a further 4 h at 37 °C and 5% CO₂. Then, the plates are read at an excitation wavelength of 560 nm and the emission at 590 nm is recorded on a SpectraMax M3 microplate reader . Background-corrected fluorescence readings are converted to cell viability data for each test well by expressing them as percentages relative to the mean negative control value^[2].

MCE 는 독립적으로 이러한 방법들의 정확성을 확인하지 않았습니다. 참고용으로만 봐주십시오.

References

[1]. Steiner MR, et al. Responses of purified phospholipases A2 to phospholipase A2 activating protein (PLAP) and Melittin. Biochim Biophys Acta. 1993 Feb 10;1166(1):124-30. [Content Brief]

[2]. Alonezi S, et al. Metabolomic Profiling of the Effects of Melittin on Cisplatin Resistant and Cisplatin Sensitive Ovarian Cancer Cells Using Mass Spectrometry and Biolog Microarray Technology. Metabolites. 2016 Oct 13;6(4). pii: E35. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	0.3513 mL	1.7566 mL	3.5131 mL	8.7828 mL
	5 mM	0.0703 mL	0.3513 mL	0.7026 mL	1.7566 mL
	10 mM	0.0351 mL	0.1757 mL	0.3513 mL	0.8783 mL
	15 mM	0.0234 mL	0.1171 mL	0.2342 mL	0.5855 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Melittin Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Melittin20449-79-0PhospholipaseInhibitorinhibitorinhibit

최근 본 상품:

상품명

Requested Quantity *

고객명 *

호칭

메일주소 *

전화번호 *

Department

회사명 *

City

도

Country or Region *

비고

상품명

Lot #

고객명 *

메일주소 *

전화번호

Department *

회사명 *

Country or Region *

비고

Name
이메일 *

	Sorry, but the email address you supplied was invalid.

Melittin

고객리뷰

Other Forms of Melittin:

Melittin Related Antibodies

16 Publications Citing Use of MCE Melittin

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Phospholipase Isoform Specific Products:

Biological Activity

Protocol

순도&문서

References

고객리뷰

Melittin Related Antibodies

몰농도 계산기

농도 희석 계산기

Complete Stock Solution Preparation Table

Melittin Related Classifications

Keywords:

최근 본 상품:

고객리뷰

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report