2. Metabolic Enzyme/Protease
  3. Phospholipase
  4. Melittin

メリチン  (Synonyms: Melittin)

製品番号: HY-P0233 純度: 99.73%
COA 取扱説明書 Technical Support

Melittin is a PLA2 activator, stimulates the activity of the low molecular weight PLA2, while it does not the increase activity of the high molecular weight PLA2.

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カスタムペプチド

CAS 番号 : 20449-79-0

容量 価格（税別） 在庫状況 数量
5 mg $150 在庫あり
10 mg $240 在庫あり
25 mg $500 在庫あり
50 mg $747 在庫あり
100 mg $1100 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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カスタマーレビュー

Based on 16 publication(s) in Google Scholar

Other Forms of Melittin:

メリチン 関連抗体

Top Publications Citing Use of Products

    Melittin purchased from MedChemExpress. Usage Cited in: Adv Healthc Mater. 2025 Apr;14(9):e2400543.  [Abstract]

    Melittin (1 μg/mL) alleviated the acute toxicity of MC complexes for FLS within 1 h.

    Melittin purchased from MedChemExpress. Usage Cited in: Adv Healthc Mater. 2025 Apr;14(9):e2400543.  [Abstract]

    Melittin (1 μg/mL). Holes in the cell membrane of FLS cultured with MC complexes decreased at 1 h, as observed by SEM.

    Melittin purchased from MedChemExpress. Usage Cited in: Adv Healthc Mater. 2025 Apr;14(9):e2400543.  [Abstract]

    The apoptosis ratio of FLS cultured with MC complexes at 4 h was increased by FCM. Melittin was used at 1 μg/mL.

    Melittin purchased from MedChemExpress. Usage Cited in: Adv Healthc Mater. 2025 Apr;14(9):e2400543.  [Abstract]

    Reduced expression of pIKKβ and pp65 in FLS cultured with MC complexes at 24 h was observed by WB with Melittin (1 μg/mL).

    Melittin purchased from MedChemExpress. Usage Cited in: Adv Healthc Mater. 2025 Apr;14(9):e2400543.  [Abstract]

    The diffuse FITC-MEL in the paw skin after FITC-MC@CSMA-MNPs attachment was observed. Scale bars represented 100 μm. CCMNPs were loaded with FITC-labeled MEL and CS (FITC-MC complexes).

    Melittin purchased from MedChemExpress. Usage Cited in: J Pharm Pharmacol. 2023 Apr 17;75(5):645-654.  [Abstract]

    Melittin (2.5, 5, 10 μg/mL; 48 h) downregulates the expression of Bcl-2, and increases the levels of the pro-apoptotic molecules Bax and cyt-c, in SW480 cells.

    Phospholipase アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Phospholipase A PLD Phospholipase C

    • 生物活性

    • プロトコル

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    Melittin is a PLA2 activator, stimulates the activity of the low molecular weight PLA2, while it does not the increase activity of the high molecular weight PLA2[1][2].

    IC50 & Target

    PLA2[1]
    Cellular Effect
    Cell Line Type Value Description References
    Caco-2 IC50
    4.5 3
    Compound: Melittin
    Cytotoxicity against human Caco2 cells after 1 hr by MTS/PMS assay
    Cytotoxicity against human Caco2 cells after 1 hr by MTS/PMS assay
    		[PMID: 24601601]
    Erythrocyte CC50
    0.2 3
    Compound: Melittin
    Hemolytic activity against human RBC assessed as concentration required to cause 50% hemolysis measured after 1 hr
    Hemolytic activity against human RBC assessed as concentration required to cause 50% hemolysis measured after 1 hr
    		[PMID: 33397096]
    Erythrocyte IC50
    0.96 3
    Compound: Melittin
    Hemolytic activity against human RBC after 1 hr
    Hemolytic activity against human RBC after 1 hr
    		[PMID: 30387611]
    HEK-293T IC50
    2.484 3
    Compound: Melittin
    Cytotoxicity against HEK293T cells assessed as cell death after 18 to 24 hrs by MTT assay
    Cytotoxicity against HEK293T cells assessed as cell death after 18 to 24 hrs by MTT assay
    		[PMID: 30742437]
    HeLa IC50
    1.9 3
    Compound: Melittin
    Cytotoxicity against human HeLa cells after 1 hr by MTS/PMS assay
    Cytotoxicity against human HeLa cells after 1 hr by MTS/PMS assay
    		[PMID: 24601601]
    HepG2 IC50
    2.5 3
    Compound: Melittin
    Cytotoxicity against human HepG2 cells after 1 hr by MTS/PMS assay
    Cytotoxicity against human HepG2 cells after 1 hr by MTS/PMS assay
    		[PMID: 24601601]
    HUVEC IC50
    13 3
    Compound: 1
    Antiangiogenic activity against VEGFA-stimulated capillary differentiation in HUVEC after 18 hrs by matrigel assay
    Antiangiogenic activity against VEGFA-stimulated capillary differentiation in HUVEC after 18 hrs by matrigel assay
    		[PMID: 23110475]
    HUVEC IC50
    18 3
    Compound: 1
    Antiangiogenic activity against VEGFA-stimulated cell proliferation in HUVEC after 48 hrs by BrdU incorporation assay
    Antiangiogenic activity against VEGFA-stimulated cell proliferation in HUVEC after 48 hrs by BrdU incorporation assay
    		[PMID: 23110475]
    IEC-6 IC50
    2.2 3
    Compound: Melittin
    Cytotoxicity against rat IEC-6 cells after 1 hr by MTS/PMS assay
    Cytotoxicity against rat IEC-6 cells after 1 hr by MTS/PMS assay
    		[PMID: 24601601]
    NIH3T3 IC50
    6.4 3
    Compound: Melittin
    Cytotoxicity against mouse NIH/3T3 cells after 1 hr by MTS/PMS assay
    Cytotoxicity against mouse NIH/3T3 cells after 1 hr by MTS/PMS assay
    		[PMID: 24601601]
    RAW264.7 IC50
    1.3 3
    Compound: Melittin
    Cytotoxicity against mouse RAW264.7 cells assessed as inhibition of cell growth at pH 6.5 measured after 24 hrs by MTT assay
    Cytotoxicity against mouse RAW264.7 cells assessed as inhibition of cell growth at pH 6.5 measured after 24 hrs by MTT assay
    		[PMID: 35294199]
    RAW264.7 IC50
    1.94 3
    Compound: Melittin
    Cytotoxicity against mouse RAW264.7 cells assessed as inhibition of cell growth at pH 7.4 measured after 24 hrs by MTT assay
    Cytotoxicity against mouse RAW264.7 cells assessed as inhibition of cell growth at pH 7.4 measured after 24 hrs by MTT assay
    		[PMID: 35294199]
    RAW264.7 IC50
    2.094 3
    Compound: Melittin
    Cytotoxicity against mouse RAW264.7 cells assessed as cell death after 18 to 24 hrs by MTT assay
    Cytotoxicity against mouse RAW264.7 cells assessed as cell death after 18 to 24 hrs by MTT assay
    		[PMID: 30742437]
    体外実験

    Melittin, an immunologically related PLA2 stimulating peptide from bee venom, increases the activity of the high molecular weight enzyme[1]. Melittin is a cytotoxic peptide from bee venom. Melittin exhibits toxicity against both A2780CR and A2780 cells, with IC50 values of 4.5 and 6.8 μg/mL, respectively. Melittin has natural anti-bacterial, anti-viral, and anti-inflammatory properties. It has also been shown to have diverse anticancer effects in several different cancer cell lines including those of gastric, breast, ovarian, liver, prostate, cervical, and lung origins. The mechanisms by which Melittin, an amphipathic haemolytic peptide, exerts its potential anticancer effects include inhibition of cell proliferation, induction of apoptosis, and direct necrosis. Melittin can also prevent EGF-induced cell invasion through its inhibition of the PI3K/Akt/mTOR signaling pathway, but this is primarily related to breast cancer cells[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    メリチン 関連抗体
    分子量

    2846.46

    分子式

    C131H229N39O31
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    Sequence

    Gly-Ile-Gly-Ala-Val-Leu-Lys-Val-Leu-Thr-Thr-Gly-Leu-Pro-Ala-Leu-Ile-Ser-Trp-Ile-Lys-Arg-Lys-Arg-Gln-Gln-NH2
    Sequence Shortening

    GIGAVLKVLTTGLPALISWIKRKRQQ-NH2
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    polypeptide, sealed storage, away from moisture and light

    *In solvent : -80°C, 6 months; -20°C, 1 month (polypeptide, sealed storage, away from moisture and light)
    溶剤 & 溶解度
    体外: 

    H2O : ≥ 50 mg/mL (17.57 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 0.3513 mL 1.7566 mL 3.5131 mL
    5 mM 0.0703 mL 0.3513 mL 0.7026 mL
    10 mM 0.0351 mL 0.1757 mL 0.3513 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

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    濃度 （終了）

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    体積 （終了）

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

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    (per animal)

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    純度とドキュメンテーション

    純度: 99.78%

    COA (242 KB) RP-HPLC (218 KB) LCMS (102 KB) Elemental Analysis Report (100 KB)

    取扱説明書 (2659 KB)
    参考文献
    細胞実験
    [2]

    Melittin is purified from bee venom by reversed phase liquid chromatography and reconstituted in sterile water to form a stock solution of 1 mg/mL before storage at -20 °C until required for analysis. Cell viability is assessed by an Alamar Blue (AB) cell viability reagent. Both A2780 and A2780CR cells are seeded at 1×104 cells/well in 96-well plates and incubated at 37 °C and 5% CO2 in a humidified atmosphere for 24 h. After this incubation period, the cells are treated with various concentrations of Melittin ranging from 0.5 to 14 µg/mL in 100 μL of medium, and re-incubated at 37 °C and 5% CO2 for a further 24 h. Triton X at 1% (v/v) and cell culture media are used as positive and negative controls, respectively. After this, AB is added at a final concentration of 10% (v/v) and the resultant mixture is incubated for a further 4 h at 37 °C and 5% CO2. Then, the plates are read at an excitation wavelength of 560 nm and the emission at 590 nm is recorded on a SpectraMax M3 microplate reader . Background-corrected fluorescence readings are converted to cell viability data for each test well by expressing them as percentages relative to the mean negative control value[2].

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 0.3513 mL 1.7566 mL 3.5131 mL 8.7828 mL
    5 mM 0.0703 mL 0.3513 mL 0.7026 mL 1.7566 mL
    10 mM 0.0351 mL 0.1757 mL 0.3513 mL 0.8783 mL
    15 mM 0.0234 mL 0.1171 mL 0.2342 mL 0.5855 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Melittin Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Melittin20449-79-0PhospholipaseInhibitorinhibitorinhibit

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    製品名:
    Melittin
    製品番号:
    HY-P0233
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