PF-04418948

Name: PF-04418948
Brand: MedChemExpress
SKU: HY-18966
Rating: 5.0 (11 reviews)

Cat. No.: HY-18966 Pureté: 99.40%: Fiche technique
COA

SDS
Instruction de manipulation
FAQs
Technical Support

PF-04418948, a chemical probe, is an orally active, potent and selective prostaglandin EP₂ receptor antagonist with an IC₅₀ of 16 nM.

Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.

CAS No. : 1078166-57-0

Size	Stock	Quantité
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	En stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	En stock	Estimated Time of Arrival: December 31
Solid
1 mg	En stock	Estimated Time of Arrival: December 31
5 mg	En stock	Estimated Time of Arrival: December 31
10 mg	En stock	Estimated Time of Arrival: December 31
50 mg	En stock	Estimated Time of Arrival: December 31
100 mg	En stock	Estimated Time of Arrival: December 31
200 mg	Obtenir un devis
500 mg	Obtenir un devis

or Bulk Inquiry

* Veuillez sélectionner la quantité avant d'ajouter des articles.

This product is a controlled substance and not for sale in your territory.

Avis client

Based on 11 publication(s) in Google Scholar

11 Publications Citing Use of MCE PF-04418948

RT-PCR

Bio/Physico-chemical Assay

In Vivo Efficacy Study

Histological Imaging/Staining

: PF-04418948 purchased from MedChemExpress. Usage Cited in: Mucosal Immunol. 2025 Apr;18(2):418-430. [Abstract]; EP2 antagonist PF-04418948 (0.5 μM) induced an increase in GzmB expression in T_reg cells.

: PF-04418948 purchased from MedChemExpress. Usage Cited in: Mucosal Immunol. 2025 Apr;18(2):418-430. [Abstract]; PKA kinase activity assay showing the effect of PF-04418948 (0.5 μM) on the PKA activity in mouse T_reg cells.

: PF-04418948 purchased from MedChemExpress. Usage Cited in: Mucosal Immunol. 2025 Apr;18(2):418-430. [Abstract]; PF-04418948 (10 mg/kg; i.p.; once daily for 10 d) administration significantly improved DSS-induced colon shortening in C57BL/6J mice.

: PF-04418948 purchased from MedChemExpress. Usage Cited in: Mucosal Immunol. 2025 Apr;18(2):418-430. [Abstract]; PF-04418948 (10 mg/kg; i.p.; once daily for 10 d) protected DSS-challenged mice from excessive inflammatory responses and intestinal tissue damage.

: PF-04418948 purchased from MedChemExpress. Usage Cited in: Mucosal Immunol. 2025 Apr;18(2):418-430. [Abstract]; PF-04418948 (10 nM-1 μM) significantly increased the expression of GzmB in T_reg cells in a dose-dependent manner.

: PF-04418948 purchased from MedChemExpress. Usage Cited in: J Virol. 2018 Sep 26;92(20):e01018-18. [Abstract]; A549 cells are added with an EP2 or an EP4 inhibitor. After 48 h, cells are subjected to WB with an anti-Axl antibody.

Recommandations en vedette

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Voir tous les produits spécifiques à Isoform Prostaglandin Receptor:

Voir toutes les isoformes

DP EP FP IP TXA₂/TP TXB₂

Activité biologique
Pureté et documentation
Références
Avis client

Description

PF-04418948, a chemical probe, is an orally active, potent and selective prostaglandin EP₂ receptor antagonist with an IC₅₀ of 16 nM^[1].

IC₅₀ & Target

EP2

16 nM (IC₅₀)

In Vitro

PF-04418948 (2 μM; 90 min) inhibits prostaglandin E2 (PGE2)-induced increase in cAMP^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	CHO cells
Concentration:	2 μM
Incubation Time:	90 min
Result:	Inhibited prostaglandin E2 (PGE2)-induced increase in cAMP in cells expressing EP2 receptors with a functional K_B value of 1.8 nM.

In Vivo

PF-04418948 (oral gavage; 1, 3, and 10 mg/kg; once) attenuats the butaprost-induced cutaneous blood flow response^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Sprague Dawley rats^[1]
Dosage:	1, 3, and 10 mg/kg
Administration:	Oral gavage; 1, 3, and 10 mg/kg; once
Result:	Reduced the peak and AUC butaprost-induced cutaneous blood flow response in a dose-dependent fashion.

Essai clinique

Masse moléculaire

409.41

Formule

C₂₃H₂₀FNO₅

CAS No.

1078166-57-0

Appearance

Solid

Color

White to off-white

SMILES

O=C(C1(COC2=CC=C3C=C(OC)C=CC3=C2)CN(C(C4=CC=C(F)C=C4)=O)C1)O

Livraison

Room temperature in continental US; may vary elsewhere.

Stockage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvant et solubilité

In Vitro:

DMSO : 50 mg/mL (122.13 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4425 mL	12.2127 mL	24.4254 mL
5 mM	0.4885 mL	2.4425 mL	4.8851 mL
10 mM	0.2443 mL	1.2213 mL	2.4425 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Calculateur de molarité
Calculateur de dilution

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 5% DMSO 40% PEG300 5% Tween-80 50% Saline
Solubility: ≥ 6.5 mg/mL (15.88 mM); Clear solution
Protocol 2

Add each solvent one by one: 2% DMSO 40% PEG300 5% Tween-80 53% Saline
Solubility: ≥ 2.6 mg/mL (6.35 mM); Clear solution
Protocol 3

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.11 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 4

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.11 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 5

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (6.11 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Pureté et documentation

Purity: 99.40%

Select Batch:

Fiche technique (279 KB) SDS (396 KB)

COA (252 KB) HNMR (265 KB) LCMS (96 KB)

Instruction de manipulation (2659 KB)

Références

[1]. af Forselles KJ, et al. In vitro and in vivo characterization of PF-04418948, a novel, potent and selective prostaglandin EP2 receptor antagonist. Br J Pharmacol. 2011 Dec;164(7):1847-1856. [Content Brief]

[2]. Birrell MA, et al. Selectivity profiling of the novel EP2 receptor antagonist, PF-04418948, in functional bioassay systems: atypical affinity at the guinea pig EP2 receptor. Br J Pharmacol. 2013 Jan;168(1):129-138. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.4425 mL	12.2127 mL	24.4254 mL	61.0635 mL
	5 mM	0.4885 mL	2.4425 mL	4.8851 mL	12.2127 mL
	10 mM	0.2443 mL	1.2213 mL	2.4425 mL	6.1063 mL
	15 mM	0.1628 mL	0.8142 mL	1.6284 mL	4.0709 mL
	20 mM	0.1221 mL	0.6106 mL	1.2213 mL	3.0532 mL
	25 mM	0.0977 mL	0.4885 mL	0.9770 mL	2.4425 mL
	30 mM	0.0814 mL	0.4071 mL	0.8142 mL	2.0354 mL
	40 mM	0.0611 mL	0.3053 mL	0.6106 mL	1.5266 mL
	50 mM	0.0489 mL	0.2443 mL	0.4885 mL	1.2213 mL
	60 mM	0.0407 mL	0.2035 mL	0.4071 mL	1.0177 mL
	80 mM	0.0305 mL	0.1527 mL	0.3053 mL	0.7633 mL
	100 mM	0.0244 mL	0.1221 mL	0.2443 mL	0.6106 mL

PF-04418948 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

PF-044189481078166-57-0PF04418948PF 04418948Prostaglandin Receptororally activityCHOInhibitorinhibitorinhibit

Vos produits récemment consultés:

Nom du produit

Requested Quantity *

Nom du demandeur *

Civilité

Adresse e-mail *

Numéro de téléphone *

Department

Nom de l'organisation *

City

État

Country or Region *

Remarques

Nom du produit

Lot #

Nom du demandeur *

Adresse e-mail *

Numéro de téléphone

Department *

Nom de l'organisation *

Country or Region *

Remarques

Nom
Email *

	Sorry, but the email address you supplied was invalid.

PF-04418948

Avis client

PF-04418948 Related Antibodies

11 Publications Citing Use of MCE PF-04418948

Recommandations en vedette

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Voir tous les produits spécifiques à Isoform Prostaglandin Receptor:

Activité biologique

Pureté et documentation

Références

Avis client

PF-04418948 Related Antibodies

Calculateur de molarité

Calculateur de dilution

Complete Stock Solution Preparation Table

PF-04418948 Related Classifications

Keywords:

Vos produits récemment consultés:

Avis client

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report