プロシアニジン B2 (Synonyms: Procyanidin B2; Proanthocyanidin B2)

Name: Procyanidin B2
Brand: MedChemExpress
SKU: HY-N0796
Rating: 5.0 (17 reviews)

製品番号: HY-N0796 純度: 99.89%: Data Sheet SDS COA 取扱説明書
FAQs
Technical Support

Procyanidin B2 is a natural flavonoid, with anti-cancer, antioxidant activities.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

CAS 番号 : 29106-49-8

容量	価格（税別）	在庫状況	数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 140	在庫あり	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 140	在庫あり	Estimated Time of Arrival: December 31
Solid
1 mg	$55	在庫あり	Estimated Time of Arrival: December 31
5 mg	$110	在庫あり	Estimated Time of Arrival: December 31
10 mg	$195	在庫あり	Estimated Time of Arrival: December 31
25 mg	$450	在庫あり	Estimated Time of Arrival: December 31
50 mg	$675	在庫あり	Estimated Time of Arrival: December 31
100 mg	$980	在庫あり	Estimated Time of Arrival: December 31
200 mg		お問い合わせ
500 mg		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 17 publication(s) in Google Scholar

Other Forms of Procyanidin B2:

Cyanidin Chloride 在庫あり
Procyanidin B1 在庫あり
Procyanidin C1 在庫あり
Procyanidin A2 在庫あり
Procyanidin A1 在庫あり
Procyanidin B3 在庫あり
Procyanidin B2 (Standard) 在庫あり

顧客検証

Histological Imaging/Staining

Flow Cytometry

IHC

Cell Imaging/Staining

: Procyanidin B2 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2022 Jul 11;13(7):594. [Abstract]; The indicated dosages of PCB2 (Procyanidin B2) were injected intraperitoneally for three consecutive days before the operation. Representative images of the H&E-stained lung tissue.

: Procyanidin B2 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2022 Jul 11;13(7):594. [Abstract]; A549 cells were treated with 20 μM PCB2 (Procyanidin B2) for 24 hours, followed by 6 hours of hypoxia and then 2 hours of reoxygenation to establish a hypoxia/reoxygenation injury model. A representative FACS scatter plot of Annexin V and propidium iodide dual labeling is shown.

: Procyanidin B2 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2022 Jul 11;13(7):594. [Abstract]; A549 cells were treated with 20 μM PCB2 (Procyanidin B2) for 24 hours, followed by 6 hours of hypoxia and then 2 hours of reoxygenation to establish a hypoxia/reoxygenation injury model. The expression of Bcl-2 protein in lung tissue was investigated.

: Procyanidin B2 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2022 Jul 11;13(7):594. [Abstract]; C57BL/6 mice were intraperitoneally injected with 30 mg/kg PCB2 (Procyanidin B2) for three consecutive days. SIRT3 immunohistochemical staining was performed.

: Procyanidin B2 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2022 Jul 11;13(7):594. [Abstract]; I/R mice were subjected to 1 hour of ischemia and 2 hours of reperfusion to induce lung I/R injury. Control mice underwent sham surgery. Preoperative intraperitoneal injections of PCB2 (Procyanidin B2: 30 mg/kg) were administered for three consecutive days prior to surgery. Representative images of A549 cells stained with TUNEL; cell nuclei were stained with DAPI.

おすすめ

•関連スクリーニングライブラリ:

•関連小分子:

•関連タンパク質:

生物活性
プロトコル
純度とドキュメンテーション
参考文献
カスタマーレビュー

製品説明

Procyanidin B2 is a natural flavonoid, with anti-cancer, antioxidant activities.

Cellular Effect

Cell Line	Type	Value	Description	References
A549	ED₅₀	> 10 μg/mL Compound: 48	Cytotoxicity against human A549 cells by tetrazolium salt-based colorimetric assay Cytotoxicity against human A549 cells by tetrazolium salt-based colorimetric assay	[PMID: 1431932]
HCT-8	ED₅₀	> 10 μg/mL Compound: 48	Cytotoxicity against human HCT8 cells by tetrazolium salt-based colorimetric assay Cytotoxicity against human HCT8 cells by tetrazolium salt-based colorimetric assay	[PMID: 1431932]
HUVEC	IC₅₀	3.8 μM Compound: 44	Inhibition of NADPH oxidase in Homo sapiens (human) HUVEC cells Inhibition of NADPH oxidase in Homo sapiens (human) HUVEC cells	10.1007/s00044-012-0353-y
KB	ED₅₀	> 10 μg/mL Compound: 48	Cytotoxicity against human KB cells by tetrazolium salt-based colorimetric assay Cytotoxicity against human KB cells by tetrazolium salt-based colorimetric assay	[PMID: 1431932]
MT4	CC₅₀	135.1 μg/mL Compound: 2, procyanidin B2	Cytotoxicity against human MT4 cells by MTT assay Cytotoxicity against human MT4 cells by MTT assay	[PMID: 10425115]
MT4	IC₅₀	17.2 μg/mL Compound: 2, procyanidin B2	Antiviral activity against HIV infected in human MT4 cells assessed as inhibition of virus-induced cytopathic effect by MTT assay Antiviral activity against HIV infected in human MT4 cells assessed as inhibition of virus-induced cytopathic effect by MTT assay	[PMID: 10425115]
TE-671	ED₅₀	> 10 μg/mL Compound: 48	Cytotoxicity against human TE671 cells by tetrazolium salt-based colorimetric assay Cytotoxicity against human TE671 cells by tetrazolium salt-based colorimetric assay	[PMID: 1431932]

体外実験

Procyanidin B2 shows antiproliferative activity to MCF-7 cells, with an IC₅₀ of 19.21 μM. However, Procyanidin B2 exhibits no effect on DNA-ladder formation^[1]. Procyanidin B2 (0.1, 1, 2 μM) inhibits the activation of pyrin domain containing 3 (NLRP3) inflammasome in human umbilical vein ECs (HUVECs), and the inhibition is via suppression of AP-1 activity, and such effect can be abolished by overexpression of c-Jun. Procyanidin B2 (2 μM for 12 h) also reduces ROS in HUVECs^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

体内実験

Procyanidin B2 (40, 20, and 10 mg/kg, p.o.) protects against cerebral ischemia-induced infarct volume and brain edema in rats. Procyanidin B2 (40 mg/kg, p.o) also improves functional outcomes, regulates blood-brain barrier (BBB) permeability after cerebral ischemia. Moreover, Procyanidin B2 attenuates cerebral ischemia-induced tight junction degradation, mitochondrial depolarization and intracellular oxidative stress. Procyanidin B2 (40 mg/kg, p.o) increases Nrf2 activation and HO-1, GSTα, and NQO1 protein expression in normal brains in vivo^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

臨床実験

分子量

578.52

分子式

C₃₀H₂₆O₁₂

CAS 番号

29106-49-8

Appearance

Solid

Color

Light yellow to light brown

SMILES

O[C@H]1[C@@H](C2=CC=C(O)C(O)=C2)OC3=CC(O)=CC(O)=C3[C@@H]1C4=C5C(C[C@@H](O)[C@@H](C6=CC=C(O)C(O)=C6)O5)=C(O)C=C4O

Structure Classification

Initial Source

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶剤 & 溶解度

体外:

H₂O : 66.67 mg/mL (115.24 mM; Need ultrasonic)

DMSO : ≥ 50 mg/mL (86.43 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.7285 mL	8.6427 mL	17.2855 mL
5 mM	0.3457 mL	1.7285 mL	3.4571 mL
10 mM	0.1729 mL	0.8643 mL	1.7285 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.32 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.32 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (4.32 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 50 mg/mL (86.43 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

純度とドキュメンテーション

純度: 99.89%

Select Batch:

データシート (287 KB) SDS (396 KB)

COA (259 KB) HNMR (290 KB) RP-HPLC (253 KB) LCMS (91 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Avelar MM, et al. Procyanidin b2 cytotoxicity to mcf-7 human breast adenocarcinoma cells. Indian J Pharm Sci. 2012 Jul;74(4):351-5. [Content Brief]

[2]. Yang H, et al. Procyanidin B2 inhibits NLRP3 inflammasome activation in human vascular endothelial cells. Biochem Pharmacol. 2014 Dec 15;92(4):599-606. [Content Brief]

[3]. Wu S, et al. Procyanidin B2 attenuates neurological deficits and blood-brain barrier disruption in a rat model of cerebral ischemia. Mol Nutr Food Res. 2015 Oct;59(10):1930-41. [Content Brief]

細胞実験 ^[1]	MCF-7 cells are grown in 5 mL of RPMI 1640 medium supplemented with 20% fetal bovine serum, penicillin (100 U/mL) and streptomycin (100 μg/mL). Cells are maintained at 37°C in a humidified atmosphere of 5% CO₂ in air and subcultured every 3 days. Exponentially growing cells are plated at a seeding density of 2×10⁴ cells/mL, in 96-well plates or in dishes. After overnight incubation to allow for attachment, the cells are exposed for 24 or 48 h to various concentrations of Procyanidin B2 (0.5; 1.0; 5.0; 10.0; 25.0 and 50.0 μM) diluted in distilled water. Two millimolar cyclophosphamide (CP) is used as the positive control and solutions are sterilised by filtration^[1]. MedChemExpress (MCE) はこれらの方法の精度を確認していません。こちらは参照専用です。
動物実験 ^[3]	To observe the effect of Procyanidin B2 on infarct size or brain edema, 40 rats are randomLy separated into five groups, and each group is administered with vehicle (equivalent dose of 0.9% saline administered via gavage) or 40, 20, or 10 mg/kg of Procyanidin B2, respectively. To elucidate the effect of Procyanidin B2 on blood-brain barrier (BBB) permeability, the rats (n = 6 per group for Evans blue extravasation and n = 4 per group for IgG leakage) are randomLy separated into two groups: vehicle and Procyanidin B2 (40 mg/kg). Procyanidin B2 (40 mg/kg) is administered intragastrically once a day starting at 3 h after middle cerebral artery occlusion (MCAO). For other observations, including immunohistological staining and western blot analysis, rats are randomLy separated into three groups: sham-operated, vehicle, and Procyanidin B2 (n = 4 per group). Procyanidin B2 (40 mg/kg) is administered intragastrically once a day starting at 3 h after MCAO. To elucidate the improvement in neurological function after ischemic stroke in rats, the rats (n = 8 per group) undergo neurobehavioral assays to evaluate the functional outcome after administration of Procyanidin B2 (40 mg/kg) once a day starting at 24 h after MCAO. The neurological deficits are assessed at 1, 3, 7, 11, and 14 days after MCAO^[3]. MedChemExpress (MCE) はこれらの方法の精度を確認していません。こちらは参照専用です。
参考文献	[1]. Avelar MM, et al. Procyanidin b2 cytotoxicity to mcf-7 human breast adenocarcinoma cells. Indian J Pharm Sci. 2012 Jul;74(4):351-5. [Content Brief] [2]. Yang H, et al. Procyanidin B2 inhibits NLRP3 inflammasome activation in human vascular endothelial cells. Biochem Pharmacol. 2014 Dec 15;92(4):599-606. [Content Brief] [3]. Wu S, et al. Procyanidin B2 attenuates neurological deficits and blood-brain barrier disruption in a rat model of cerebral ischemia. Mol Nutr Food Res. 2015 Oct;59(10):1930-41. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	1.7285 mL	8.6427 mL	17.2855 mL	43.2137 mL
	5 mM	0.3457 mL	1.7285 mL	3.4571 mL	8.6427 mL
	10 mM	0.1729 mL	0.8643 mL	1.7285 mL	4.3214 mL
	15 mM	0.1152 mL	0.5762 mL	1.1524 mL	2.8809 mL
	20 mM	0.0864 mL	0.4321 mL	0.8643 mL	2.1607 mL
	25 mM	0.0691 mL	0.3457 mL	0.6914 mL	1.7285 mL
	30 mM	0.0576 mL	0.2881 mL	0.5762 mL	1.4405 mL
	40 mM	0.0432 mL	0.2161 mL	0.4321 mL	1.0803 mL
	50 mM	0.0346 mL	0.1729 mL	0.3457 mL	0.8643 mL
	60 mM	0.0288 mL	0.1440 mL	0.2881 mL	0.7202 mL
	80 mM	0.0216 mL	0.1080 mL	0.2161 mL	0.5402 mL
H₂O	100 mM	0.0173 mL	0.0864 mL	0.1729 mL	0.4321 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Procyanidin B2 Related Classifications

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量		濃度		体積		分子量 *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）	×	体積 _（開始）	=	濃度 _（終了）	×	体積 _（終了）
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Procyanidin B229106-49-8Proanthocyanidin B2Procyanidin B 2Procyanidin B-2Proanthocyanidin B 2Proanthocyanidin B-2Reactive Oxygen Species (ROS)Inhibitorinhibitorinhibit

最近チェックした製品:

製品名

カスタマ需要量 *

お名前 *

タイトル

メールアドレス *

電話番号 *

デパートメント

組纖名 *

市区町村

都道府県

国或いは地域 *

必ず会社名を記載ください。個人への返信は行いません。

備考

製品名

Lot #

お名前 *

メールアドレス *

電話番号 *

部門 *

組纖名 *

国或いは地域 *

備考

お名前
メール *

	Sorry, but the email address you supplied was invalid.

プロシアニジン B2 (Synonyms: Procyanidin B2; Proanthocyanidin B2)

カスタマーレビュー

Other Forms of Procyanidin B2:

プロシアニジン B2 関連抗体

顧客検証

おすすめ

•関連スクリーニングライブラリ:

•関連小分子:

•関連タンパク質:

生物活性

プロトコル

純度とドキュメンテーション

参考文献

カスタマーレビュー

プロシアニジン B2 関連抗体

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Procyanidin B2 Related Classifications

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

Keywords:

最近チェックした製品:

カスタマーレビュー

Request for HNMR Report

Request for HNMR Report