Procyanidin C1 (Synonyms: PCC1)

Name: Procyanidin C1
Brand: MedChemExpress
SKU: HY-N2342
Rating: 5.0 (8 reviews)

Cat. No.: HY-N2342 Purity: 99.54%: Data Sheet
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Procyanidin C1 (PCC1), a natural polyphenol with oral activity, causes DNA damage, cell cycle arrest and induces apoptosis. Procyanidin C1 decreases the level of Bcl-2, but enhances BAX, caspase 3 and 9 expression in cancer cells. Procyanidin C1 shows senotherapeutic activity and increases lifespan in mice.

For research use only. We do not sell to patients.

CAS No. : 37064-30-5

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
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Based on 8 publication(s) in Google Scholar

Other Forms of Procyanidin C1:

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Procyanidin B2 In-stock
Procyanidin A2 In-stock
Procyanidin A1 In-stock
Procyanidin B3 In-stock
Procyanidin C1 (Standard) Get quote

8 Publications Citing Use of MCE Procyanidin C1

RT-PCR

Cell Proliferation/Viability Assay

Cell Migration/Invasion Assay

In Vivo Efficacy Study

: Procyanidin C1 purchased from MedChemExpress. Usage Cited in: J Adv Res. 2024 Jun:60:215-231. [Abstract]; Procyanidin C1 (PCC1) affected the level of hsa-miR-501-3p.

: Procyanidin C1 purchased from MedChemExpress. Usage Cited in: J Adv Res. 2024 Jun:60:215-231. [Abstract]; The subsequent experiments showed that Procyanidin C1 (PCC1, 5-10 μM, 24-72 h) inhibited CRC cells proliferation in a dose- and time-dependent manners.

: Procyanidin C1 purchased from MedChemExpress. Usage Cited in: J Adv Res. 2024 Jun:60:215-231. [Abstract]; In addition to proliferation, the effects of Procyanidin C1 (PCC1, 5-10 μM, 0-48 h) on cell migration and invasion were observed by using wound healing assays. The results showed that PCC1 inhibited the migration and invasion of DLD1 and HCT116 compared with the control groups.

: Procyanidin C1 purchased from MedChemExpress. Usage Cited in: J Adv Res. 2024 Jun:60:215-231. [Abstract]; In addition to proliferation, the effects of Procyanidin C1 (PCC1, 5-10 μM) on cell migration and invasion were observed by using transwell assays. The results showed that PCC1 inhibited the migration and invasion of DLD1 and HCT116 compared with the control groups.

: Procyanidin C1 purchased from MedChemExpress. Usage Cited in: J Adv Res. 2024 Jun:60:215-231. [Abstract]; To investigate whether the results of in vitro experiments were also observed in the in vivo experiments, mice were inoculated with cells, and CDDP (2 mg/kg and 4 mg/kg via i.p. injection) or Procyanidin C1 (PCC1, 20 mg/kg and 40 mg/kg via i.p. injection) was injected, then the weight and volume of tumors were measured respectively. The results showed that PCC1 significantly inhibited tumor weight and volume compared with the control group, although it was less effective than CDDP.

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Biological Activity
Purity & Documentation
References
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Description

IC₅₀ & Target

IC50: 31.5 μg/mL (MCF-7), 36.6 μg/mL (MDA-MB-231)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
A549	ED₅₀	>10 μg/mL Compound: 49	Cytotoxicity against human A549 cells by tetrazolium salt-based colorimetric assay Cytotoxicity against human A549 cells by tetrazolium salt-based colorimetric assay	[PMID: 1431932]
HCT-8	ED₅₀	>10 μg/mL Compound: 49	Cytotoxicity against human HCT8 cells by tetrazolium salt-based colorimetric assay Cytotoxicity against human HCT8 cells by tetrazolium salt-based colorimetric assay	[PMID: 1431932]
KB	ED₅₀	>10 μg/mL Compound: 49	Cytotoxicity against human KB cells by tetrazolium salt-based colorimetric assay Cytotoxicity against human KB cells by tetrazolium salt-based colorimetric assay	[PMID: 1431932]
MT4	CC₅₀	16.5 μg/mL Compound: 10, proanthocyanidin C1	Cytotoxicity against human MT4 cells by MTT assay Cytotoxicity against human MT4 cells by MTT assay	[PMID: 10425115]
MT4	IC₅₀	4.3 μg/mL Compound: 10, proanthocyanidin C1	Antiviral activity against HIV infected in human MT4 cells assessed as inhibition of virus-induced cytopathic effect by MTT assay Antiviral activity against HIV infected in human MT4 cells assessed as inhibition of virus-induced cytopathic effect by MTT assay	[PMID: 10425115]
TE-671	ED₅₀	>10 μg/mL Compound: 49	Cytotoxicity against human TE671 cells by tetrazolium salt-based colorimetric assay Cytotoxicity against human TE671 cells by tetrazolium salt-based colorimetric assay	[PMID: 1431932]

In Vitro

Procyanidin C1 (6.25-100 μg/mL; 48 h) shows cytotoxic activities to MCF-7 and MDA-MB-231 cells^[1].
Procyanidin C1 (35 μg/mL; 48 h) affects cell cycle of MCF-7 and MDA-MB-231 cancer cells^[1].
Procyanidin C1 significantly up-regulates Chk 1 and Chk 2 in MCF-7 and MDA-MB-231 cancer cells^[1].
Procyanidin C1 (27.85 and 66.41 μL) induces significant DNA damage in MCF-7 and MDA-MB-231 cancer cells^[1].
Procyanidin C1 (45 μg/mL; 72 h) reduces the expression level of Bcl-2 but increases the expression level of BAX, and increases activities of caspase 3 and 9 to induces cell apoptosis of MCF-7 and MDA-MB-231 cancer cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cycle Analysis^[1]

Cell Line:	MCF-7 and MDA-MB-231 cell lines
Concentration:	35 μg/mL
Incubation Time:	48 h
Result:	Induced the cell cycle arrest at the S-phase in MCF-7 and MDA-MB-231 cell lines.

Cell Cytotoxicity Assay^[1]

Cell Line:	MCF-7 and MDA-MB-231 cell lines
Concentration:	6.25-100 μg/mL
Incubation Time:	48 h
Result:	Inhibited cell proliferation of MCF-7 and MDA-MB-231 cells with IC₅₀ values of 31.5 and 36.6 μg/mL and showed a higher cytotoxic activity to MDA-MB-231 than tamoxifen.

In Vivo

Procyanidin C1 (20 mg/kg; i.p.; 2 weeks after the first MIT dose and then delivered biweekly) increases tumour regression^[2].
Procyanidin C1 (20 mg/kg; i.p.; for 7 d) shows senolytic efficacy in mice with senescent mouse embryonic fibroblasts injection^[2].
Procyanidin C1 (20 mg/kg; p.o.; for 3 d) increases the lifespan of old mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Non-obese diabetes and severe combined immunodficiency mice with PSC27 and PC3 cancer cells injection, and pre-treated with mitoxantrone (MIT)^[2]
Dosage:	20 mg/kg
Administration:	Intraperitoneal injection; 20 mg/kg; 2 weeks after the first MIT dose and then delivered biweekly
Result:	Remarkably enhanced tumour regression (55.2% reduction in tumour size compared with MIT alone; 74.9% reduction in tumour volume compared with the placebo treatment) and depleted the majority of senescent cells in chemotherapy treated animals.

Animal Model:	24-27 months of age mice (both sexes)^[2]
Dosage:	20 mg/kg
Administration:	Oral gavage; 20 mg/kg; for three consecutive days
Result:	Enhanced the median post-treatment lifespan with 64.2% and decreased the mortality hazard than the vehicle-treated group.

Clinical Trial

Molecular Weight

866.77

Formula

C₄₅H₃₈O₁₈

CAS No.

37064-30-5

Appearance

Solid

Color

Light yellow to yellow

SMILES

OC1=C2C(O[C@H](C3=CC(O)=C(O)C=C3)[C@H](O)[C@H]2C4=C5C(C[C@@H](O)[C@@H](C6=CC(O)=C(O)C=C6)O5)=C(O)C=C4O)=C([C@H]7C8=C(O)C=C(O)C=C8O[C@H](C9=CC(O)=C(O)C=C9)[C@@H]7O)C(O)=C1

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (115.37 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.1537 mL	5.7685 mL	11.5371 mL
5 mM	0.2307 mL	1.1537 mL	2.3074 mL
10 mM	0.1154 mL	0.5769 mL	1.1537 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (2.88 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (2.88 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (2.88 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 6.25 mg/mL (7.21 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.54%

Select Batch:

Data Sheet (287 KB) SDS (393 KB)

COA (264 KB) HNMR (348 KB) LCMS (88 KB)

Handling Instructions (2659 KB)

References

[1]. Koteswari LL, et al. A comparative anticancer study on procyanidin C1 against receptor positive and receptor negative breast cancer. Nat Prod Res. 2019 Jan 8:1-8. [Content Brief]

[2]. Xu Q, et al. The flavonoid procyanidin C1 has senotherapeutic activity and increases lifespan in mice. Nat Metab. 2021 Dec;3(12):1706-1726. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.1537 mL	5.7685 mL	11.5371 mL	28.8427 mL
	5 mM	0.2307 mL	1.1537 mL	2.3074 mL	5.7685 mL
	10 mM	0.1154 mL	0.5769 mL	1.1537 mL	2.8843 mL
	15 mM	0.0769 mL	0.3846 mL	0.7691 mL	1.9228 mL
	20 mM	0.0577 mL	0.2884 mL	0.5769 mL	1.4421 mL
	25 mM	0.0461 mL	0.2307 mL	0.4615 mL	1.1537 mL
	30 mM	0.0385 mL	0.1923 mL	0.3846 mL	0.9614 mL
	40 mM	0.0288 mL	0.1442 mL	0.2884 mL	0.7211 mL
	50 mM	0.0231 mL	0.1154 mL	0.2307 mL	0.5769 mL
	60 mM	0.0192 mL	0.0961 mL	0.1923 mL	0.4807 mL
	80 mM	0.0144 mL	0.0721 mL	0.1442 mL	0.3605 mL
	100 mM	0.0115 mL	0.0577 mL	0.1154 mL	0.2884 mL

Procyanidin C1 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Procyanidin C1 (Synonyms: PCC1)

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Other Forms of Procyanidin C1:

Procyanidin C1 Related Antibodies

8 Publications Citing Use of MCE Procyanidin C1

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Customer Review

Procyanidin C1 Related Antibodies

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Procyanidin C1 Related Classifications

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