STAT5-IN-1

Name: STAT5-IN-1
Brand: MedChemExpress
SKU: HY-101853
Rating: 5.0 (31 reviews)

Cat. No.: HY-101853 Pureté: 98.35%: Fiche technique
COA

SDS
Instruction de manipulation
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STAT5-IN-1 is a STAT5 inhibitor with an IC₅₀ of 47 μM for STAT5β isoform.

Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.

CAS No. : 285986-31-4

Size	Stock	Quantité
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	En stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	En stock	Estimated Time of Arrival: December 31
Solid
5 mg	En stock	Estimated Time of Arrival: December 31
10 mg	En stock	Estimated Time of Arrival: December 31
25 mg	En stock	Estimated Time of Arrival: December 31
50 mg	En stock	Estimated Time of Arrival: December 31
100 mg	En stock	Estimated Time of Arrival: December 31
200 mg	Obtenir un devis
500 mg	Obtenir un devis

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Based on 31 publication(s) in Google Scholar

Other Forms of STAT5-IN-1:

STAT5-IN-1 (Standard) Obtenir un devis

31 Publications Citing Use of MCE STAT5-IN-1

Flow Cytometry

Apoptosis Analysis

RT-PCR

: STAT5-IN-1 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2026 Jan 23;17(1):952. [Abstract]; Frequency of IL-4 and IL-17A production in CD4+ T cells from DKO and TKO mice cultured in vitro under TH0 to TH17 conditions with the treatment of STAT5-IN (1, 10, 50 μM).

: STAT5-IN-1 purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2024 Dec 18;9(1):352. [Abstract]; Western blotting analysis of AHR expression in STAT5-IN-1 (1, 5, 10 μM)-treated peritoneal macrophages, with or without 4T1-CM treatment for 24 h.

: STAT5-IN-1 purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2024 Dec 18;9(1):352. [Abstract]; mRNA levels of Ahr in peritoneal macrophages treated with or without 4T1-CM, in the presence or absence of STAT5-IN-1 (1, 5, or 10 μM) for 24 h.

: STAT5-IN-1 purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2022 Mar 30;8(1):142. [Abstract]; STAT5-IN-1 (25, 50 μM) was added to CD103⁺ cDC cultures at the indicated concentration for 9 days after which apoptosis levels were analyzed by flow cytometry.

Recommandations en vedette

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Voir tous les produits spécifiques à Isoform STAT:

Voir toutes les isoformes

STAT3 STAT5 STAT6 STAT1

Activité biologique
Pureté et documentation
Références
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Description

STAT5-IN-1 is a STAT5 inhibitor with an IC₅₀ of 47 μM for STAT5β isoform.

IC₅₀ & Target

STAT5β

47 μM (IC₅₀)

Cellular Effect

Cell Line	Type	Value	Description	References
Neutrophil	IC₅₀	> 1000 μM Compound: 18	Antiinflammatory activity in human neutrophils assessed as inhibition of respiratory burst Antiinflammatory activity in human neutrophils assessed as inhibition of respiratory burst	[PMID: 20576329]

In Vitro

The signal transducer and activator of transcription 5 (STAT5) is a member of the STAT family of proteins, implicated in cell growth and differentiation. STAT5-IN-1 inhibits STAT5 by binding to the SH2 domain. The functions of the SH2 domains of STAT3, STAT1, and of the tyrosine kinase Lck are inhibited to a lesser extent (IC50>500 μM). STAT5-IN-1 block STAT5/STAT5 DNA binding in K562 nuclear extracts. Substitution of the hydrogen at C6 of the chromone ring by an ethyl group does not affect activity of STAT5-IN-1 against STAT5β, but leads to complete loss of selectivity against other STAT family members^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Masse moléculaire

293.28

Formule

C₁₆H₁₁N₃O₃

CAS No.

285986-31-4

Appearance

Solid

Color

White to light yellow

SMILES

O=C(C1=CC=CN=C1)N/N=C/C2=COC3=CC=CC=C3C2=O

Livraison

Room temperature in continental US; may vary elsewhere.

Stockage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	1 year
	-20°C	6 months

Solvant et solubilité

In Vitro:

DMSO : 20 mg/mL (68.19 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.4097 mL	17.0486 mL	34.0971 mL
5 mM	0.6819 mL	3.4097 mL	6.8194 mL
10 mM	0.3410 mL	1.7049 mL	3.4097 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Calculateur de molarité
Calculateur de dilution

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2 mg/mL (6.82 mM); Clear solution

This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 50% PEG300 50% Saline
Solubility: 12.5 mg/mL (42.62 mM); Suspended solution; Need ultrasonic
Protocol 2

Add each solvent one by one: 0.5% CMC-Na/saline water
Solubility: 12.5 mg/mL (42.62 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Pureté et documentation

Purity: 98.35%

Select Batch:

Fiche technique (272 KB) SDS (393 KB)

COA (247 KB) HNMR (386 KB) LCMS (92 KB)

Instruction de manipulation (2659 KB)

Références

[1]. Müller J, et al. Discovery of chromone-based inhibitors of the transcription factor STAT5. Chembiochem. 2008 Mar 25;9(5):723-7. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.4097 mL	17.0486 mL	34.0971 mL	85.2428 mL
	5 mM	0.6819 mL	3.4097 mL	6.8194 mL	17.0486 mL
	10 mM	0.3410 mL	1.7049 mL	3.4097 mL	8.5243 mL
	15 mM	0.2273 mL	1.1366 mL	2.2731 mL	5.6829 mL
	20 mM	0.1705 mL	0.8524 mL	1.7049 mL	4.2621 mL
	25 mM	0.1364 mL	0.6819 mL	1.3639 mL	3.4097 mL
	30 mM	0.1137 mL	0.5683 mL	1.1366 mL	2.8414 mL
	40 mM	0.0852 mL	0.4262 mL	0.8524 mL	2.1311 mL
	50 mM	0.0682 mL	0.3410 mL	0.6819 mL	1.7049 mL
	60 mM	0.0568 mL	0.2841 mL	0.5683 mL	1.4207 mL

STAT5-IN-1 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

STAT5-IN-1285986-31-4STATInhibitorinhibitorinhibit

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STAT5-IN-1

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Other Forms of STAT5-IN-1:

STAT5-IN-1 Related Antibodies

31 Publications Citing Use of MCE STAT5-IN-1

Recommandations en vedette

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Voir tous les produits spécifiques à Isoform STAT:

Activité biologique

Pureté et documentation

Références

Avis client

STAT5-IN-1 Related Antibodies

Calculateur de molarité

Calculateur de dilution

Complete Stock Solution Preparation Table

STAT5-IN-1 Related Classifications

Keywords:

Vos produits récemment consultés:

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SAFETY DATA SHEET (SDS)

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