2. Autophagy Apoptosis Cell Cycle/DNA Damage
  3. Autophagy Apoptosis Deubiquitinase
  4. Spautin-1

Spautin-1 is a specific and potent autophagy inhibitor which inhibits ubiquitin-specific peptidases, USP10 and USP13 with IC50s of 0.6-0.7 μM.

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CAS 番号 : 1262888-28-7

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>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 67 在庫あり
Solution
10 mM * 1 mL in DMSO USD 67 在庫あり
Solid
5 mg $61 在庫あり
10 mg $77 在庫あり
25 mg $162 在庫あり
50 mg $275 在庫あり
100 mg $380 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

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カスタマーレビュー

Based on 24 publication(s) in Google Scholar

Spautin-1 関連抗体

Top Publications Citing Use of Products

    Spautin-1 purchased from MedChemExpress. Usage Cited in: ACS Nano. 2025 Feb 11;19(5):5659-5679.  [Abstract]

    TAK-243/Spautin-1 groups results consistently support a positive correlation between the activity of HSP90, ubiquitination, and proteasome system with the disassembly of NSGs.

    Spautin-1 purchased from MedChemExpress. Usage Cited in: Sci Adv. 2025 Sep 26;11(39):eadx3827.  [Abstract]

    HEK293T cells transfected with the indicated plasmid combinations were treated with Spautin-1 (10 μM) for 6 hours, Before collection, cells were treated with MG132 for 6 hours. Immunoblot analysis of NLRP3 ubiquitination in cell lysates immunoprecipitated with anti–c-Myc beads.

    Spautin-1 purchased from MedChemExpress. Usage Cited in: Cancer Immunol Immunother. 2025 Aug 25;74(9):293.  [Abstract]

    Cell viability of SU-DHL-4, SU-DHL-6, OCI-LY3, OCI-Ly10, and A20 cell lines detected by a CCK-8 kit after Spautin-1 (0.1, 0.3, 1, 3, 10, 30, 100 μM, 24, 48 h) treatment for the indicated time.

    Spautin-1 purchased from MedChemExpress. Usage Cited in: Cancer Immunol Immunother. 2025 Aug 25;74(9):293.  [Abstract]

    H&E staining showed that Spautin-1 (20 mg/kg, i.p.) induced tumor cell shrinkage, fragmentation, and disorganized tissue architecture.

    Spautin-1 purchased from MedChemExpress. Usage Cited in: Cancer Immunol Immunother. 2025 Aug 25;74(9):293.  [Abstract]

    Spautin-1 Spautin-1 (20 mg/kg, i.p.) treatment induced the infiltration of CD3+/CD4+ and CD8+ T cells into the tumor tissues.

    Spautin-1 purchased from MedChemExpress. Usage Cited in: Cell Death Differ. 2023 Feb;30(2):527-543.  [Abstract]

    ESCC cells were treated with different concentrations of Spautin-1 (0, 20, 40, 80 μM) for 24 h, and then western blotting was used to detect the protein levels of ANLN and USP10.

    Deubiquitinase アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    USP1 USP2 USP4 USP7 USP8 USP10 USP21 USP30 UCHL1 USP11 USP15 USP28 USP25

    • 生物活性

    • プロトコル

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    Spautin-1 is a specific and potent autophagy inhibitor which inhibits ubiquitin-specific peptidases, USP10 and USP13 with IC50s of 0.6-0.7 μM.

    IC50 & Target

    USP10

    0.6-0.7 μM (IC50)

    Cellular Effect
    Cell Line Type Value Description References
    MIA PaCa-2 EC50
    20 μM
    Compound: Spautin-1
    Antiproliferative activity against human MIA PaCa-2 cells assessed as cell viability measured after 72 hrs by MTT assay
    Antiproliferative activity against human MIA PaCa-2 cells assessed as cell viability measured after 72 hrs by MTT assay
    		[PMID: 28105281]
    PC-9 IC50
    >5 μM
    Compound: 5
    Cytotoxicity against human PC-9 cells harboring EGFR mutant assessed as inhibition of cell growth incubated for 72 hrs by ATP-based luminescence CellTiter-Glo assay
    Cytotoxicity against human PC-9 cells harboring EGFR mutant assessed as inhibition of cell growth incubated for 72 hrs by ATP-based luminescence CellTiter-Glo assay
    		[PMID: 36410591]
    体外実験

    Spautin-1 enhances imatinib mesylate (IM)-induced CmL cell apoptosis by reducing the expression of the anti-apoptotic proteins Mcl-1 and Bcl-2. The pro-apoptotic activity of spautin-1 is associated with activation of GSK3β, an important downstream effector of PI3K/AKT. Spautin-1 enhances IM-induced cytotoxicity in CmL cell line K562, decreasing the IC50 from 1 to 0.5 μM[1]. The mechanism of spautin-1 acting on acute pancreatitis is associated with impaired autophagy inhibition[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Spautin-1 関連抗体
    体内実験

    Spautin-1 ameliorates the pathogenesis of acute pancreatitis induced by cerulein or L-arginine. Spautin-1 pretreatment significantly diminishes the elevation of serum amylase and lipase levels, which are indicative of trypsin activity. Increasing levels of serum TNFα caused by cerulein are inhibited in the presence of spautin-1. Spautin-1 treatment can ameliorate the inflammation damage induced by cerulein, such as edema, degeneration, coagulative necrosis and infiltration of inflammatory cells[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    分子量

    271.26

    分子式

    C15H11F2N3
    CAS 番号
    Appearance

    Solid

    Color

    White to light yellow

    SMILES

    FC1=CC2=C(N=CN=C2C=C1)NCC3=CC=C(F)C=C3
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : 50 mg/mL (184.33 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.6865 mL 18.4325 mL 36.8650 mL
    5 mM 0.7373 mL 3.6865 mL 7.3730 mL
    10 mM 0.3687 mL 1.8433 mL 3.6865 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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    一般には略語で表示されます：C1V1 = C2V2

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    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (9.22 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 2.5 mg/mL (9.22 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  50% PEG300    50% PBS

      Solubility: 10 mg/mL (36.87 mM); Suspended solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Please enter your animal formula composition:
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    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション
    参考文献
    細胞実験
    [1]

    Spautin-1 is dissolved in DMSO. Cell proliferation is evaluated using CCK-8 kit. K562 cells (1x105/mL) are seeded into 96-well plates in triplicate and then treated with 125 to 4,000 nM IM alone or in combi¬nation with spautin-1 (10 μM). After 48 h of incubation, 10 μL of CCK-8 reagent is added to each well. Four hours later, the absorbance is read at 450 nm using a microplate reader[1].

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
    動物実験
    [2]

    Mice: In this study, mice models with acute pancreatitis, including cerulein- and L-arginine-induced models, are constructed. For the cerulein-induced model, four intraperitoneal injections of cerulein (50 μg/kg body weight) are given consecutively at hourly intervals; The L-arginine-induced model received hourly intraperitoneal injections of 1.4 g/kg (optimal dosage for this study) L-arginine three times[2].

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.6865 mL 18.4325 mL 36.8650 mL 92.1625 mL
    5 mM 0.7373 mL 3.6865 mL 7.3730 mL 18.4325 mL
    10 mM 0.3686 mL 1.8432 mL 3.6865 mL 9.2162 mL
    15 mM 0.2458 mL 1.2288 mL 2.4577 mL 6.1442 mL
    20 mM 0.1843 mL 0.9216 mL 1.8432 mL 4.6081 mL
    25 mM 0.1475 mL 0.7373 mL 1.4746 mL 3.6865 mL
    30 mM 0.1229 mL 0.6144 mL 1.2288 mL 3.0721 mL
    40 mM 0.0922 mL 0.4608 mL 0.9216 mL 2.3041 mL
    50 mM 0.0737 mL 0.3686 mL 0.7373 mL 1.8432 mL
    60 mM 0.0614 mL 0.3072 mL 0.6144 mL 1.5360 mL
    80 mM 0.0461 mL 0.2304 mL 0.4608 mL 1.1520 mL
    100 mM 0.0369 mL 0.1843 mL 0.3686 mL 0.9216 mL
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    Spautin-1 Related Classifications

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
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      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Spautin-11262888-28-7Spautin1Spautin 1AutophagyApoptosisDeubiquitinaseDUBsInhibitorinhibitorinhibit

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    製品名:
    Spautin-1
    製品番号:
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