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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Art. -Nr. Produktname Wirkung Reinheit Chemical Structure
  • HY-W751238
    Dimethyl methylene blue zinc chloride double salt
    		99.64%
    Dimethyl methylene blue zinc chloride double salt (DMMB) is a cationic dye. Dimethyl methylene blue zinc chloride double salt competitively binds to the active site of the PANI-Benz-Ag nanocomposite with endotoxin, releasing DMMB and increasing the fluorescence intensity. Dimethyl methylene blue zinc chloride double salt can be used as a fluorescent probe for the detection of bacteria (such as E. Coli) endotoxin. Dimethyl methylene blue zinc chloride double salt can be used for the quantification of sulfated glycosaminoglycans.
    Dimethyl methylene blue zinc chloride double salt
  • HY-D0084
    3,3'-Dihexyloxacarbocyanine iodide
    		99.62%
    3,3'-Dihexyloxacarbocyanine iodide is a carbocyanine dye which can be used to monitor changes in mitochondrial membrane potential.
    3,3'-Dihexyloxacarbocyanine iodide
  • HY-P2528
    Enhanced Green Fluorescent Protein (EGFP) (200-208)
    		98.91%
    Enhanced Green Fluorescent Protein (EGFP) (200-208) is a marker gene product derived from the jellyfish Aequorea Victoria. Enhanced Green Fluorescent Protein (EGFP) (200-208) is a common reporter protein and is easy to detect.
    Enhanced Green Fluorescent Protein (EGFP) (200-208)
  • HY-159529
    DBCO-PEG4-Eosin 5-isothiocyanate
    DBCO-PEG4-Eosin 5-isothiocyanate (DBCO-PEG4-Eosin Y) is a fluorescent probe. DBCO-PEG4-Eosin 5-isothiocyanate contains a clickable functional group, DBCO, and four PEG units. DBCO-PEG4-Eosin 5-isothiocyanate can be used for various imaging detections.
    DBCO-PEG4-Eosin 5-isothiocyanate
  • HY-NP0132
    Griffonia Simplicifolia Lectin I Isolectin B4 (Fluorescein)
    Griffonia Simplicifolia Lectin I Isolectin B4 (GSL I Isolectin B4) Fluorescein is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.).Griffonia Simplicifolia Lectin I Isolectin B4 (GSL I Isolectin B4) Fluorescein is a biological material or organic compound that can be used in life science research.
    Griffonia Simplicifolia Lectin I Isolectin B4 (Fluorescein)
  • HY-D0221
    2,4,6-Tri-2-pyridinyl-1,3,5-triazine
    		99.87%
    2,4,6-Tri-2-pyridinyl-1,3,5-triazine (NSC 112125) is a colorimetric reagent for detecting iron. 2,4,6-Tri-2-pyridinyl-1,3,5-triazine forms a complex with Fe (II) and can be quantified as a measure of iron concentration by colorimetric detection at 594 nm.
    2,4,6-Tri-2-pyridinyl-1,3,5-triazine
  • HY-D0722
    5(6)-CFDA
    		98.75%
    5(6)-CFDA is a common aliphatic luciferin-line organism. CFDA conducts free diffusion into cells, and then it is hydrolyzed into carboxyl fluorescein (CF) by intracellular non-specific lipase. CF containing portion contains an additional negative charge so that it is better retained in cells, compared to fluorescein dyes.
    5(6)-CFDA
  • HY-162543
    18:1 Liss Rhod PE
    		99%
    18:1 Liss Rhod (18:1 Lissamine rhodamine) PE is a fluorescent phospholipid and fluorescent probe.18:1 Liss Rhod PE admixes into phospholipid inks for large-scale monitoring of dip-pen nanolithography-generated lithographic structures via fluorescence microscopy.18:1 Liss Rhod PE undergoes phase separation or self-quenching under certain conditions in thin lipid membrane stacks.
    18:1 Liss Rhod PE
  • HY-D0817
    Rhodamine 110
    		99.81%
    Rhodamine 110 is a sensitive and selective substrate for assaying proteinases in solution or inside living cells. The excitation wavelength is 498 nm and the emission wavelength is 521 nm.
    Rhodamine 110
  • HY-D0193
    Ponceau 4R
    		99.84%
    Ponceau 4R is a synthetic colorant that can be used for food coloring. Ponceau 4R is a strawberry red azo dye that can be used in a variety of foods, usually synthesized from aromatic hydrocarbons, and is stable to light, heat, and acids.
    Ponceau 4R
  • HY-D0006
    Bathophenanthroline
    		99.59%
    Bathophenanthroline is a nitrogen-containing organic ligand that can form stable complexes with transition metal ions such as Ru2+. In proteomics, Bathophenanthroline is often used for in-gel protein staining. The negatively charged sulfonic acid groups of Bathophenanthroline interact electrostatically with the positively charged amino acid residues of proteins, thereby achieving specific binding to proteins and enhancing the fluorescent signal of detection. Bathophenanthroline can be used for protein separation and detection in SDS-PAGE gels in the field of proteomics, as well as other fluorescence studies[1]. Ex/Em=532 nm/610 nm[1].
    Bathophenanthroline
  • HY-DY1002
    H2DCFDA (solution)
    H2DCFDA (DCFH-DA) (solution) is a cell-permeable probe used to detect intracellular reactive oxygen species (ROS) (Ex/Em=488/525 nm) .
    Solvent and concentration: DMSO: 10 mM.
    The 1 mL volume is defined as the base specification. All larger sizes correspond to incremental volumes of this base.
    H2DCFDA (solution)
  • HY-D2295
    Mito-FerroGreen
    		98%
    Mito-FerroGreen is a mitochondria-specific ferrous ion detection probe with an excitation wavelength of 488nm.
    Mito-FerroGreen
  • HY-D1553
    BODIPY-C12
    		99.90%
    BODIPY-C12 is a boron-dipyrromethene derivative containing a lipophilic tail (excitation 490 nm). BODIPY-C12 is a hydrophobic molecular and sensitivity to the surrounding environment and can be used to quantify viscosity, critical micelle concentration, and critical micelle temperature.
    BODIPY-C12
  • HY-D1781
    NTPAN-MI
    		98.78%
    NTPAN-MI is a fluorescent probe (excitation wavelength: 405 nm). NTPAN-MI is selectively activated after labeling unfolded proteins with exposed thiols, thereby reporting the degree of protein homeostasis.
    NTPAN-MI
  • HY-P0028
    Phalloidin
    		99.83%
    Phalloidin is a mushroom-derived toxin which can be used to label F-actin of the cytoskeleton with fluorochrome .
    Phalloidin
  • HY-D0935A
    4-Methylumbelliferyl-β-D-glucuronide hydrate
    		99.92%
    4-Methylumbelliferyl-β-D-glucuronide hydrate is a fluorogenic substrat (λex=362 nm, λem=445 nm). 4-Methylumbelliferyl-β-D-glucuronide hydrate has potential applications in detecting the activity of β-glucuronidase and the number of Escherichia coli.
    4-Methylumbelliferyl-β-D-glucuronide hydrate
  • HY-D0232
    Brilliant Blue R250
    Brilliant blue R250 (Brilliant Blue R), an anionic dye, is the most popular stain to detect proteins resolved in SDS-PAGE gels.
    Brilliant Blue R250
  • HY-D0823
    CY3-SE
    Cy3-SE (Sulfo-Cy3 NHS ester; Sulfo Cyanine3 NHS ester) is a sulfonated cyanine dye-derived fluorescent labeling reagent with λex of about 515 nm and λem of about 568 nm. Cy3-SE can interact with the π-π stacking of biomolecules (e.g., nucleoside monophosphates, proteins), inhibiting the photoisomerization process and increasing the fluorescence quantum yield and lifetime.
    CY3-SE
  • HY-D0056
    5-Carboxyfluorescein diacetate N-succinimidyl ester
    		99.30%
    5-Carboxyfluorescein diacetate N-succinimidyl ester is a cell permeable dye (Ex=492 nm, Em=517 nm). 5-Carboxyfluorescein diacetate N-succinimidyl ester can label cells by covalently binding to intracellular molecules. 5-Carboxyfluorescein diacetate N-succinimidyl ester is used to track lymphocyte migration and proliferation.
    5-Carboxyfluorescein diacetate N-succinimidyl ester
Art. -Nr. Produktname / Synonyms Application Reactivity