1. Rhodamine 110

Rhodamine 110  (Synonyms: Rhodamine 110 chloride; RH110)

Cat. No.: HY-D0817 Purity: 98.04%
COA Handling Instructions

Rhodamine 110 is a sensitive and selective substrate for assaying proteinases in solution or inside living cells. The excitation wavelength is 498 nm and the emission wavelength is 521 nm.

For research use only. We do not sell to patients.

Rhodamine 110 Chemical Structure

Rhodamine 110 Chemical Structure

CAS No. : 13558-31-1

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
USD 55 In-stock
Solution
10 mM * 1 mL in DMSO USD 55 In-stock
Solid
50 mg USD 50 In-stock
100 mg USD 70 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Description

Rhodamine 110 is a sensitive and selective substrate for assaying proteinases in solution or inside living cells. The excitation wavelength is 498 nm and the emission wavelength is 521 nm[1].

In Vitro

Rhodamine 110 accumulates in mitochondria in a cationic form, which alters the pH in this cellular compartment. Rhodamine 110 accumulates in human lymphoblastoid cells and Friend leukemia cells. No cytotoxicity to human lymphoblastoid cells is observed below 10 μM, but Rhodamine 110 causes Friend leukemia cells to die at a concentrations above 100 μM[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Rhodamine 110 is less toxic than the parent molecule based on the intravenous LD50 acute toxicity values of 89.5 mg/kg and 140.0 mg/kg for Rhodamine B and Rhodamine 110, respectively. Both molecules induce liver and kidney enlargement after ingestion, and male rats show more significant increases than female rats after Rhodamine 110 exposure. In addition, testis weight increased in male rats dosed with Rhodamine 110. The pharmacokinetics of Rhodamine 110 are assessed following oral administration at two dosages (3 and 10 mg/kg) and intravenous administration at one dosage (3 mg/kg). Pharmacokinetic parameters are calculated using an extravascular input and IV-bolus input, noncompartmental model analysis conducted with WinNonlin Standard Edition. The pharmacokinetic parameters of Rhodamine 110 indicates that the maximum plasma concentrations (Cmax) of the two oral dosages are 283.4 and 657.0 ng/mL, which are reached at 140 and 210 min, respectively. This indicates that Rhodamine 110 absorption is not rapid after ingestion, as it took over 2 h to be absorbed from the intestines into the blood. The areas under the concentration–time curves (AUCs) for the two dosages are 138.1±20.3 and 444.0±170.8 h ng/mL. The pharmacokinetic data demonstrate that the AUC is proportional to the administered oral dose of Rhodamine 110 (3 mg/kg and 10 mg/kg). Furthermore, the clearance (Cl) of the two orally administered doses is 7.94 and 8.61 mL/min/kg, respectively[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

366.80

Formula

C20H15ClN2O3

CAS No.
Appearance

Solid

Color

Brown to red

SMILES

O=C(C1=CC=CC=C1C2=C3C=CC(N)=CC3=[O+]C4=C2C=CC(N)=C4)O.[Cl-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 30 mg/mL (81.79 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7263 mL 13.6314 mL 27.2628 mL
5 mM 0.5453 mL 2.7263 mL 5.4526 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 98.04%

Animal Administration
[1]

Rats[1]
Adult male Sprague-Dawley rats (230±20 g) are used. After the rats recovered, Rhodamine 110 in polyethylene glycol 400 (0.3 and 1 mg/mL) is administered at 3 mg/kg and 10 mg/kg by oral gavage. Blood samples (150 μL) are collected from the right jugular vein 5, 15, 30, 60, 120, 180, 240, 300, 360, 480, and 720 min after drug administration. After each sampling, 100 μL of normal saline is administered via catheter to compensate for the loss of body fluid, and a 50 μL heparin solution (20 IU heparin/mL normal saline) is provided to prevent coagulation. Blood samples are centrifuged at 16,000g for 10 min at 4 °C to obtain plasma, which is stored at -20 °C until analysis. After surgery, Rhodamine 110 in polyethylene glycol 400 (1 mg/mL) is administered intravenously to rats at 3 mg/kg (n=6). A 150 μL blood sample is collected from the right jugular vein 5, 15, 30, 60, 120, 180, 240, 300, 360, 480, and 720 min after drug administration. Then, 100 μL of normal saline is administered via the right jugular vein to compensate for body fluid loss, and 50 μL of a heparin solution (20 IU heparin/mL normal saline) is provided to prevent blood clotting.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.7263 mL 13.6314 mL 27.2628 mL 68.1570 mL
5 mM 0.5453 mL 2.7263 mL 5.4526 mL 13.6314 mL
10 mM 0.2726 mL 1.3631 mL 2.7263 mL 6.8157 mL
15 mM 0.1818 mL 0.9088 mL 1.8175 mL 4.5438 mL
20 mM 0.1363 mL 0.6816 mL 1.3631 mL 3.4079 mL
25 mM 0.1091 mL 0.5453 mL 1.0905 mL 2.7263 mL
30 mM 0.0909 mL 0.4544 mL 0.9088 mL 2.2719 mL
40 mM 0.0682 mL 0.3408 mL 0.6816 mL 1.7039 mL
50 mM 0.0545 mL 0.2726 mL 0.5453 mL 1.3631 mL
60 mM 0.0454 mL 0.2272 mL 0.4544 mL 1.1360 mL
80 mM 0.0341 mL 0.1704 mL 0.3408 mL 0.8520 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Rhodamine 110
Cat. No.:
HY-D0817
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