Pharmacokinetics of Rhodamine 110 and Its Organ Distribution in Rats

  • J Agric Food Chem. 2017 Sep 6;65(35):7797-7804. doi: 10.1021/acs.jafc.7b02685.
Shiau-Han Jiang  1 ,  Yung-Yi Cheng  2 ,  Teh-Ia Huo  1 ,  Tung-Hu Tsai  2  3  4  5
Affiliations
  • 1. Institute of Pharmacology, National Yang-Ming University , Taipei, Taiwan.
  • 2. Institute of Traditional Medicine, National Yang-Ming University , Taipei, Taiwan.
  • 3. Graduate Institute of Acupuncture Science, China Medical University , Taichung, Taiwan.
  • 4. School of Pharmacy, College of Pharmacy, Kaohsiung Medical University , Kaohsiung, Taiwan.
  • 5. Department of Chemical Engineering, National United University , Miaoli, Taiwan.
PMID: 28793756 DOI: 10.1021/acs.jafc.7b02685
Abstract

Rhodamine dyes have been banned as food additives due to their potential tumorigenicity. Rhodamine 110 is illegal as a food additive, although its pharmacokinetics have not been characterized, and no accurate bioanalytical methods are available to quantify rhodamine 110. The aim of this study was to develop and validate a fast, stable, and sensitive method to quantify rhodamine 110 using high-performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS) to assess its pharmacokinetics and organ distribution in awake rats. Rhodamine 110 exhibited linear pharmacokinetics and slow elimination after oral administration. Furthermore, its oral bioavailability was approximately 34-35%. The distribution in the liver and kidney suggests that these organs are primarily responsible for rhodamine 110 metabolism and elimination. Our investigation describes the pharmacokinetics and a quantification method for rhodamine 110, improving our understanding of the food safety of rhodamine dyes.

Keywords
HPLC-MS/MS; food additives; pharmacokinetics; rhodamine 110; rhodamine dyes; toxicokinetics.
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