Bathophenanthroline
Based on 1 Customer Validation
Bathophenanthroline is a nitrogen-containing organic ligand that can form stable complexes with transition metal ions such as Ru2+. In proteomics, Bathophenanthroline is often used for in-gel protein staining. The negatively charged sulfonic acid groups of Bathophenanthroline interact electrostatically with the positively charged amino acid residues of proteins, thereby achieving specific binding to proteins and enhancing the fluorescent signal of detection. Bathophenanthroline can be used for protein separation and detection in SDS-PAGE gels in the field of proteomics, as well as other fluorescence studies[1]. Ex/Em=532 nm/610 nm[1].
For research use only. We do not sell to patients.
- Purity: 98.79%
- CAS No.: 1662-01-7
- Formula: C24H16N2
- Molecular Weight:332.40
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Storage:
RT, protect from light, stored under nitrogen.
In solvent -80°C, 1 year , -20°C, 6 months
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| Huh-7 | CC50 |
>100 μM
Compound: GNF-Pf-4554
|
NOVARTIS: Cytotoxicity against human hepatocellular carcinoma cell line (Huh7)
NOVARTIS: Cytotoxicity against human hepatocellular carcinoma cell line (Huh7)
|
[PMID: 18579783] |
In SDS-PAGE gel staining experiments, heteroligand ruthenium(II) complexes containing bathophenanthroline (BP) and bathophenanthroline disulfonate (BPS) shows higher sensitivity than SYPRO Ruby for protein detection at near-neutral pH and reduces artificial post-translational modifications[1].
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Fix gels in 7% acetic acid/10% ethanol.
2. Stain overnight with 0.5-1 mg/L Ru(BPS)2(BP) or Ru(BPS)(BP)2 in acidic/neutral buffer, destained.
3. Scan for fluorescence at 532 nm excitation/610 nm emission.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1662-01-7
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Appearance Solid
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Molecular Weight 332.40
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Formula C24H16N2
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Color Off-white to light yellow
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SMILES
C12=C(N=CC=C3C4=CC=CC=C4)C3=CC=C1C(C5=CC=CC=C5)=CC=N2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
RT, protect from light, stored under nitrogen
In solvent -80°C 1 year -20°C 6 months
Solvent & Solubility
DMSO : 2.78 mg/mL (8.36 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : < 0.1 mg/mL (insoluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (275 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.0084 mL | 15.0421 mL | 30.0842 mL | 75.2106 mL |
| 5 mM | 0.6017 mL | 3.0084 mL | 6.0168 mL | 15.0421 mL |
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