3,3'-Dihexyloxacarbocyanine iodide
Based on 1 publication(s) in Google Scholar
3,3'-Dihexyloxacarbocyanine iodide is a carbocyanine dye which can be used to monitor changes in mitochondrial membrane potential.
For research use only. We do not sell to patients.
- Purity: 99.17%
- CAS No.: 53213-82-4
- Formula: C29H37IN2O2
- Molecular Weight:572.52
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) 3,3'-Dihexyloxacarbocyanine iodide
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Biological Activity
Addition of cells to the cuvette containing 0.25 μM 3,3'-Dihexyloxacarbocyanine iodide (DiOCg(3)) leads to an increase in fluorescence, equilibration is rapid and is complete by 4 min. When f-met-leu-phe (10-7 M) is added to neutrophils preequilibrated with 3,3'-Dihexyloxacarbocyanine iodide, there is a short lag period of 5 seconds. The lag period is followed by a rapid loss of fluorescence. Examination of 3,3'-Dihexyloxacarbocyanine iodide loaded neutrophils using fluorescence microscopy demonstrates that in resting neutrophils the brightest fluorescence is associated with long thin organelles which corresponds to the distribution of mitochondria[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Chemical Information
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CAS No. 53213-82-4
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Appearance Solid
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Molecular Weight 572.52
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Formula C29H37IN2O2
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Color Orange to red
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SMILES
CCCCCCN1/C(OC2=CC=CC=C12)=C/C=C/C3=[N+](CCCCCC)C4=CC=CC=C4O3.[I-]
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Synonyms
DiOC6(3) iodide
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (1)
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Journal Impact Factor
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Most Recent
Solvent & Solubility
DMSO : 33.33 mg/mL (58.22 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
Protocol
Neutrophils (2.5×106/mL) are preequilibrated in 0.25 μM 3,3'-Dihexyloxacarbocyanine iodide (DiOCg(3)) for 5 min at 37°C in a 1 cm path length cuvette. Final volume is 2 mL. Fluorescence measurements are made in a spectrofluorimeter (emission wavelength: 510 nm, excitation wavelength: 470 nm). Stimuli are injected by a syringe through an injection port on the spectrofluorimeter, and their volumes never exceed 5% (v/v)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (279 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.7467 mL | 8.7333 mL | 17.4666 mL | 43.6666 mL |
| 5 mM | 0.3493 mL | 1.7467 mL | 3.4933 mL | 8.7333 mL | |
| 10 mM | 0.1747 mL | 0.8733 mL | 1.7467 mL | 4.3667 mL | |
| 15 mM | 0.1164 mL | 0.5822 mL | 1.1644 mL | 2.9111 mL | |
| 20 mM | 0.0873 mL | 0.4367 mL | 0.8733 mL | 2.1833 mL | |
| 25 mM | 0.0699 mL | 0.3493 mL | 0.6987 mL | 1.7467 mL | |
| 30 mM | 0.0582 mL | 0.2911 mL | 0.5822 mL | 1.4556 mL | |
| 40 mM | 0.0437 mL | 0.2183 mL | 0.4367 mL | 1.0917 mL | |
| 50 mM | 0.0349 mL | 0.1747 mL | 0.3493 mL | 0.8733 mL |
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