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  2. 3,3'-Dihexyloxacarbocyanine iodide

3,3'-Dihexyloxacarbocyanine iodide (Synonyms: DiOC6(3) iodide)

Cat. No.: HY-D0084 Purity: ≥98.0%
Handling Instructions

3,3'-Dihexyloxacarbocyanine iodide is a carbocyanine dye which can be used to monitor changes in mitochondrial membrane potential.

For research use only. We do not sell to patients.

3,3'-Dihexyloxacarbocyanine iodide Chemical Structure

3,3'-Dihexyloxacarbocyanine iodide Chemical Structure

CAS No. : 53213-82-4

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Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

3,3'-Dihexyloxacarbocyanine iodide is a carbocyanine dye which can be used to monitor changes in mitochondrial membrane potential.

In Vitro

Addition of cells to the cuvette containing 0.25 μM 3,3'-Dihexyloxacarbocyanine iodide (DiOCg(3)) leads to an increase in fluorescence, equilibration is rapid and is complete by 4 min. When f-met-leu-phe (10-7 M) is added to neutrophils preequilibrated with 3,3'-Dihexyloxacarbocyanine iodide, there is a short lag period of 5 seconds. The lag period is followed by a rapid loss of fluorescence. Examination of 3,3'-Dihexyloxacarbocyanine iodide loaded neutrophils using fluorescence microscopy demonstrates that in resting neutrophils the brightest fluorescence is associated with long thin organelles which corresponds to the distribution of mitochondria[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

572.52

Formula

C₂₉H₃₇IN₂O₂

CAS No.

53213-82-4

SMILES

CCCCCCN1/C(OC2=CC=CC=C12)=C/C=C/C3=[N+](CCCCCC)C4=CC=CC=C4O3.[I-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 62.5 mg/mL (109.17 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.7467 mL 8.7333 mL 17.4666 mL
5 mM 0.3493 mL 1.7467 mL 3.4933 mL
10 mM 0.1747 mL 0.8733 mL 1.7467 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Cell Assay
[1]

Neutrophils (2.5×106/mL) are preequilibrated in 0.25 μM 3,3'-Dihexyloxacarbocyanine iodide (DiOCg(3)) for 5 min at 37°C in a 1 cm path length cuvette. Final volume is 2 mL. Fluorescence measurements are made in a spectrofluorimeter (emission wavelength: 510 nm, excitation wavelength: 470 nm). Stimuli are injected by a syringe through an injection port on the spectrofluorimeter, and their volumes never exceed 5% (v/v)[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

Purity: ≥98.0%

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Keywords:

3,3'-DihexyloxacarbocyanineDiOC6(3)OthersInhibitorinhibitorinhibit

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3,3'-Dihexyloxacarbocyanine iodide
Cat. No.:
HY-D0084
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