Dimethyl methylene blue zinc chloride double salt  (Synonyms: DMMB)

Dimethyl methylene blue zinc chloride double salt (DMMB) is a cationic dye. Dimethyl methylene blue zinc chloride double salt competitively binds to the active site of the PANI-Benz-Ag nanocomposite with endotoxin, releasing DMMB and increasing the fluorescence intensity. Dimethyl methylene blue zinc chloride double salt can be used as a fluorescent probe for the detection of bacteria (such as E. Coli) endotoxin^[1]. Dimethyl methylene blue zinc chloride double salt can be used for the quantification of sulfated glycosaminoglycans^[2].

Dimethyl methylene blue zinc chloride double salt can be used as a fluorescent probe for the detection of bacteria (such as E. Coli) endotoxin, the detection protocol is as follows^[1]:
1. Sample Preparation: Incubate the sample with a solution of Dimethyl methylene blue zinc chloride double salt (DMMB) to allow it completely binding to Lipopolysaccharide (LPS) (HY-D1056), forming a fluorescence‑quenched complex.
2. Introduction of the Nanocomposite Sensor: Add a defined quantity of PANI‑benzimidazole‑Ag nanocomposite to the mixture.
3. Competitive Binding and Displacement: Owing to its higher affinity for endotoxin, the nanocomposite displaces LPS from the Dimethyl methylene blue zinc chloride double salt-LPS complex, leading to the recovery of fluorescence.
4. Fluorescence Detection and Analysis: Monitor the reaction area using confocal fluorescence microscopy. Record fluorescence images and quantify the signal recovery to determine endotoxin concentration.
Dimethyl methylene blue zinc chloride double salt can be used for the quantification of sulfated glycosaminoglycans^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

416.04

C₁₈H₂₂N₃S.1/2ZnCl₄

931418-92-7

Solid

[Cl-][Zn+2]([Cl-])([Cl-])[Cl-].CC1=CC(N(C)C)=CC2=[S+]C(C=C(N(C)C)C=C3C)=C3N=C12.[1/2]

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

• [1]. Alemu T A, et al. Highly sensitive detection of bacteria (E. Coli) endotoxin using novel PANI-benzimidazole-Ag nanocomposite by DMMB dye displacement assay[J]. Materials Research Express, 2023, 10(7): 075302.

  [2]. Ladner YD, et al. The Dimethylmethylene Blue Assay (DMMB) for the Quantification of Sulfated Glycosaminoglycans. Methods Mol Biol. 2023;2598:115-121.  [Content Brief]