Dimethyl methylene blue zinc chloride double salt
Based on 1 Customer Validation
Dimethyl methylene blue zinc chloride double salt (DMMB) is a cationic dye. Dimethyl methylene blue zinc chloride double salt competitively binds to the active site of the PANI-Benz-Ag nanocomposite with endotoxin, releasing DMMB and increasing the fluorescence intensity. Dimethyl methylene blue zinc chloride double salt can be used as a fluorescent probe for the detection of bacteria (such as E. Coli) endotoxin. Dimethyl methylene blue zinc chloride double salt can be used for the quantification of sulfated glycosaminoglycans.
For research use only. We do not sell to patients.
- Purity : 99.64%
- CAS No.: 931418-92-7
- Formula: C18H22N3S.1/2ZnCl4
- Molecular Weight:416.04
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Dimethyl methylene blue zinc chloride double salt can be used as a fluorescent probe for the detection of bacteria (such as E. Coli) endotoxin, the detection protocol is as follows[1]:
1. Sample Preparation: Incubate the sample with a solution of Dimethyl methylene blue zinc chloride double salt (DMMB) to allow it completely binding to Lipopolysaccharide (LPS) (HY-D1056), forming a fluorescence‑quenched complex.
2. Introduction of the Nanocomposite Sensor: Add a defined quantity of PANI‑benzimidazole‑Ag nanocomposite to the mixture.
3. Competitive Binding and Displacement: Owing to its higher affinity for endotoxin, the nanocomposite displaces LPS from the Dimethyl methylene blue zinc chloride double salt-LPS complex, leading to the recovery of fluorescence.
4. Fluorescence Detection and Analysis: Monitor the reaction area using confocal fluorescence microscopy. Record fluorescence images and quantify the signal recovery to determine endotoxin concentration.
Dimethyl methylene blue zinc chloride double salt can be used for the quantification of sulfated glycosaminoglycans[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 931418-92-7
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Appearance Solid
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Molecular Weight 416.04
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Formula C18H22N3S.1/2ZnCl4
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SMILES
[Cl-][Zn+2]([Cl-])([Cl-])[Cl-].CC1=CC(N(C)C)=CC2=[S+]C(C=C(N(C)C)C=C3C)=C3N=C12.[1/2]
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Synonyms
DMMB
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
In Vitro:
H2O : 5.83 mg/mL (14.01 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (276 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 2.4036 mL | 12.0181 mL | 24.0361 mL | 60.0904 mL |
| 5 mM | 0.4807 mL | 2.4036 mL | 4.8072 mL | 12.0181 mL | |
| 10 mM | 0.2404 mL | 1.2018 mL | 2.4036 mL | 6.0090 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Dimethyl methylene blue zinc chloride double salt Related Classifications
HY-W751238 Related Classifications
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