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  4. 2,4,6-Tri-2-pyridinyl-1,3,5-triazine

2,4,6-Tri-2-pyridinyl-1,3,5-triazine  (Synonyms: NSC 112125; 2,4,6-Tipyidyl-s-tiazie)

Cat. No.: HY-D0221 Purity: 99.87%
COA
SDS
Handling Instructions Technical Support

2,4,6-Tri-2-pyridinyl-1,3,5-triazine (NSC 112125) is a colorimetric reagent for detecting iron. 2,4,6-Tri-2-pyridinyl-1,3,5-triazine forms a complex with Fe (II) and can be quantified as a measure of iron concentration by colorimetric detection at 594 nm.

For research use only. We do not sell to patients.

2,4,6-Tri-2-pyridinyl-1,3,5-triazine

2,4,6-Tri-2-pyridinyl-1,3,5-triazine Chemical Structure

CAS No. : 3682-35-7

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2,4,6-Tri-2-pyridinyl-1,3,5-triazine Related Antibodies

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Description

2,4,6-Tri-2-pyridinyl-1,3,5-triazine (NSC 112125) is a colorimetric reagent for detecting iron. 2,4,6-Tri-2-pyridinyl-1,3,5-triazine forms a complex with Fe (II) and can be quantified as a measure of iron concentration by colorimetric detection at 594 nm[1].

In Vitro

Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
Reagent Preparation: Prepare a 10 g/L 2,4,6-Tris(2-pyridyl)-1,3,5-triazine (TPTZ) solution by dissolving TPTZ in a small amount of hydrochloric acid or ethanol.
Sample Preparation: Mix the iron-containing sample with a reducing agent (e.g., 1 mL of 100 g/L ascorbic acid) to ensure all iron is in the Fe(II) state.
Complexation: Add 1 mL of the TPTZ solution and 1 mL of an appropriate buffer (e.g., 1 M ammonium acetate, pH 5.4).
Measurement: Dilute to volume (e.g., 10 or 25 mL), allow it to stand for color development (approx. 10-30 min), and measure the absorbance of the blue [Fe(TPTZ)?]2? complex at 593 nm.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

2,4,6-Tri-2-pyridinyl-1,3,5-triazine Related Antibodies
Molecular Weight

312.34

Formula

C18H12N6
CAS No.
Appearance

Solid

Color

White to yellow

SMILES

C1(C2=NC=CC=C2)=NC(C3=NC=CC=C3)=NC(C4=NC=CC=C4)=N1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Store at room temperature 3 years

In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

Methanol : 50 mg/mL (160.08 mM; Need ultrasonic)

DMSO : 2 mg/mL (6.40 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.2016 mL 16.0082 mL 32.0164 mL
5 mM 0.6403 mL 3.2016 mL 6.4033 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.87%

SDS (393 KB)

COA (253 KB) HNMR (131 KB) RP-HPLC (212 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / Methanol 1 mM 3.2016 mL 16.0082 mL 32.0164 mL 80.0410 mL
5 mM 0.6403 mL 3.2016 mL 6.4033 mL 16.0082 mL
Methanol 10 mM 0.3202 mL 1.6008 mL 3.2016 mL 8.0041 mL
15 mM 0.2134 mL 1.0672 mL 2.1344 mL 5.3361 mL
20 mM 0.1601 mL 0.8004 mL 1.6008 mL 4.0020 mL
25 mM 0.1281 mL 0.6403 mL 1.2807 mL 3.2016 mL
30 mM 0.1067 mL 0.5336 mL 1.0672 mL 2.6680 mL
40 mM 0.0800 mL 0.4002 mL 0.8004 mL 2.0010 mL
50 mM 0.0640 mL 0.3202 mL 0.6403 mL 1.6008 mL
60 mM 0.0534 mL 0.2668 mL 0.5336 mL 1.3340 mL
80 mM 0.0400 mL 0.2001 mL 0.4002 mL 1.0005 mL
100 mM 0.0320 mL 0.1601 mL 0.3202 mL 0.8004 mL
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2,4,6-Tri-2-pyridinyl-1,3,5-triazine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

2,4,6-Tri-2-pyridinyl-1,3,5-triazine3682-35-7NSC 112125 2,4,6-Tipyidyl-s-tiazieNSC112125NSC-112125MOFsFluorescent DyeMetal-Organic FrameworksColor substrateindicatoriron concentrationInhibitorinhibitorinhibit

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