2. Immunology/Inflammation NF-κB Metabolic Enzyme/Protease
  3. Reactive Oxygen Species (ROS)
  4. DPPH

DPPH  (Synonyms: 2,2-Diphenyl-1-picrylhydrazyl)

Cat. No.: HY-112053 Purity: 98.77%
COA
SDS
Handling Instructions Technical Support

DPPH (2,2-Diphenyl-1-picrylhydrazyl) is a stable free radical that can be used to measure the radical scavenging activity of antioxidants. The odd electron of nitrogen atom in DPPH is reduced by receiving a hydrogen atom from antioxidants to the corresponding hydrazine. DPPH method may be utilized in aqueous and nonpolar organic solvents and can be used to examine both hydrophilic and lipophilic antioxidants.

For research use only. We do not sell to patients.

CAS No. : 1898-66-4

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Based on 46 publication(s) in Google Scholar

Other Forms of DPPH:

DPPH Related Antibodies

Top Publications Citing Use of Products

46 Publications Citing Use of MCE DPPH

Bio/Physico-chemical Assay

    DPPH purchased from MedChemExpress. Usage Cited in: Bioact Mater. 2025 Jun 7:52:200-212.  [Abstract]

    DPPH (100 μM; 30 min). DPPH scavenging capabilities of PCL, PCL-PDA, PCL-PDA-GM, and PCL-PDA-GM + NIR scaffolds.

    DPPH purchased from MedChemExpress. Usage Cited in: Mater Today Bio. 2025 Feb 20:31:101598.  [Abstract]

    DPPH (300 μM; 30 min). Scavenging rates of DPPH radicals scavenged by TA-Fe NPs at different concentrations.

    DPPH purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2024 Oct 24;22(1):653.  [Abstract]

    DPPH (20 μM; 30 min). Quantitative analysis of the scavenging rate of DPPH radicals by Cur, CurNPs, and HA-CurNPs.

    DPPH purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2023 Feb;10(6):e2204006.  [Abstract]

    DPPH (100 μM; 30 min). DPPH assay to test the ability of 5‐HT (10 µM) as radical trapping agent, fer‐1 (10 µM), Lipro‐1 (10 µM), and Vitamin E (10 µM) as positive controls.

    DPPH purchased from MedChemExpress. Usage Cited in: ACS Appl Mater Interfaces. 2021 Oct 6;13(39):46391-46405.  [Abstract]

    DPPH (32.5 μM; 30 min). Antioxidant activity was measured by DPPH assay.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    DPPH (2,2-Diphenyl-1-picrylhydrazyl) is a stable free radical that can be used to measure the radical scavenging activity of antioxidants. The odd electron of nitrogen atom in DPPH is reduced by receiving a hydrogen atom from antioxidants to the corresponding hydrazine. DPPH method may be utilized in aqueous and nonpolar organic solvents and can be used to examine both hydrophilic and lipophilic antioxidants[1].

    In Vitro

    DPPH shows a strong absorption band at 517 nm due to its odd electron and solution appears a deep violet colour, the absorption vanishes as the electron pairs off. The resulting decolorization is stoichiometric with respect to the number of electrons taken up. The alcoholic solutions of 0.5 mM are densely colored and at this concentration, the Lambert-Beer law is obeyed over the useful range of absorption[1].
    DPPH assay is a rapid, simple, inexpensive and widely used method to measure the ability of compounds to act as free radical scavengers or hydrogen donors, and to evaluate antioxidant activity of foods. It can also be used to quantify antioxidants in complex biological systems, for solid or liquid samples. This method is easy and applies to measure the overall antioxidant capacity and the free radical scavenging activity of fruit and vegetable juice. This assay has been successfully utilized for investigating antioxidant properties of wheat grain and bran, vegetables, conjugated linoleic acids, herbs, edible seed oils, and flours in several different solvent systems including ethanol, aqueous acetone, methanol, aqueous alcohol and benzene. It is a convenient method for the antioxidant assay of cysteine, glutathione, ascorbic acid, tocopherol and polyhydroxy aromatic compounds, for olive oil, fruits, juices and wines[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    DPPH Related Antibodies
    Molecular Weight

    394.32

    Formula

    C18H12N5O6
    CAS No.
    Appearance

    Solid

    Color

    Brown to black

    SMILES

    O=[N+](C1=C([N]N(C2=CC=CC=C2)C3=CC=CC=C3)C([N+]([O-])=O)=CC([N+]([O-])=O)=C1)[O-]
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 25 mg/mL (63.40 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.5360 mL 12.6801 mL 25.3601 mL
    5 mM 0.5072 mL 2.5360 mL 5.0720 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

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    (per animal)

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation

    Purity: 98.81%

    SDS (419 KB)

    COA (254 KB) RP-HPLC (206 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.5360 mL 12.6801 mL 25.3601 mL 63.4003 mL
    5 mM 0.5072 mL 2.5360 mL 5.0720 mL 12.6801 mL
    10 mM 0.2536 mL 1.2680 mL 2.5360 mL 6.3400 mL
    15 mM 0.1691 mL 0.8453 mL 1.6907 mL 4.2267 mL
    20 mM 0.1268 mL 0.6340 mL 1.2680 mL 3.1700 mL
    25 mM 0.1014 mL 0.5072 mL 1.0144 mL 2.5360 mL
    30 mM 0.0845 mL 0.4227 mL 0.8453 mL 2.1133 mL
    40 mM 0.0634 mL 0.3170 mL 0.6340 mL 1.5850 mL
    50 mM 0.0507 mL 0.2536 mL 0.5072 mL 1.2680 mL
    60 mM 0.0423 mL 0.2113 mL 0.4227 mL 1.0567 mL
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    DPPH Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    DPPH1898-66-42,2-Diphenyl-1-picrylhydrazylReactive Oxygen Species (ROS)Inhibitorinhibitorinhibit

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