Enhanced Green Fluorescent Protein (EGFP) (200-208)

Name: Enhanced Green Fluorescent Protein (EGFP) (200-208)
Brand: MedChemExpress
SKU: HY-P2528
Rating: 4.5 (0 reviews)

Cat. No.: HY-P2528 Pureza: 98.91%: Ficha de datos
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Enhanced Green Fluorescent Protein (EGFP) (200-208) is a marker gene product derived from the jellyfish Aequorea Victoria. Enhanced Green Fluorescent Protein (EGFP) (200-208) is a common reporter protein and is easy to detect.

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Enhanced Green Fluorescent Protein (EGFP) (200-208) Estructura química

No. CAS : 323198-39-6

Tamaño	Stock	Cantidad
1 mg	En stock	Estimated Time of Arrival: December 31
5 mg	En stock	Estimated Time of Arrival: December 31
10 mg	En stock	Estimated Time of Arrival: December 31
50 mg	Obtener un presupuesto
100 mg	Obtener un presupuesto

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Descripciòn

In Vitro

Enhanced Green Fluorescent Protein (EGFP) (200-208) is a specific peptide of enhanced green fluorescent protein (EGFP), containing 9 amino acids, and is the immunodominant epitope of EGFP. Enhanced Green Fluorescent Protein (EGFP) (200-208) can be presented by the H-2Kd molecule. The EGFP 200-208-H-2Kd complex can serve as an antigen for T cells that will be specifically recognized and killed by the T cell receptor (TCR)^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Peso molecular

1019.11

Fòrmula

C₄₅H₇₀N₁₂O₁₅

No. CAS

323198-39-6

Appearance

Solid

Color

White to off-white

Sequence

His-Tyr-Leu-Ser-Thr-Gln-Ser-Ala-Leu

Sequence Shortening

HYLSTQSAL

Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

Sealed storage, away from moisture and light, under nitrogen

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)

Solvente y solubilidad

In Vitro:

H₂O

Peptide Solubility and Storage Guidelines:

1. Calculate the length of the peptide.

2. Calculate the overall charge of the entire peptide according to the following table:

	Contents	Assign value
Acidic amino acid	Asp (D), Glu (E), and the C-terminal -COOH.	-1
Basic amino acid	Arg (R), Lys (K), His (H), and the N-terminal -NH₂	+1
Neutral amino acid	Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q)	0

3. Recommended solution:

Overall charge of peptide	Details
Negative (<0)	1. Try to dissolve the peptide in water first. 2. If water fails, add NH₄OH (<50 μL). 3. If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0)	1. Try to dissolve the peptide in water first. 2. If water fails, try dissolving the peptide in a 10%-30% acetic acid solution. 3. If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0)	1. Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first. 2. For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.

Pureza y Documentación

Purity: 98.91%

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Ficha de datos (268 KB) SDS (252 KB)

COA (240 KB) RP-HPLC (267 KB) MS (199 KB) Elemental Analysis Report (100 KB)

Instrucciones de manejo (2659 KB)

Referencias

[1]. J Steitz, et al. Balb/c EGFP mice are tolerant against immunization utilizing recombinant adenoviral-based vectors encoding EGFP: a novel model for the study of tolerance mechanisms and vaccine efficacy. Mol Immunol. 2010 Feb;47(5):1149-53. [Content Brief]

[2]. Agudo J, et al., GFP-specific CD8 T cells enable targeted cell depletion and visualization of T-cell interactions. Nat Biotechnol. 2015 Dec;33(12):1287-1292. [Content Brief]

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	0.9812 mL	4.9062 mL	9.8125 mL	24.5312 mL
	5 mM	0.1962 mL	0.9812 mL	1.9625 mL	4.9062 mL
	10 mM	0.0981 mL	0.4906 mL	0.9812 mL	2.4531 mL
	15 mM	0.0654 mL	0.3271 mL	0.6542 mL	1.6354 mL
	20 mM	0.0491 mL	0.2453 mL	0.4906 mL	1.2266 mL
	25 mM	0.0392 mL	0.1962 mL	0.3925 mL	0.9812 mL
	30 mM	0.0327 mL	0.1635 mL	0.3271 mL	0.8177 mL
	40 mM	0.0245 mL	0.1227 mL	0.2453 mL	0.6133 mL