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  3. Fluorescent Dye
  4. CellTracker Blue CMAC

CellTracker Blue CMAC is a non-fluorescent cell membrane permeable dye. The chloromethyl groups of CellTracker Blue CMAC are enzymatically cleaved by intracellular glutathione (GSH) to generate a fluorescent product (blue fluorescence, Ex/Em: 360/460 nm). CellTracker Blue CMAC is suitable for long-term cell tracking (up to 72 hours) and cell proliferation studies, and can also quantify GSH levels.

For research use only. We do not sell to patients.

CAS No. : 147963-22-2

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
5 mg In-stock
10 mg In-stock
25 mg In-stock
50 mg In-stock
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Customer Review

Based on 7 publication(s) in Google Scholar

Other Forms of CellTracker Blue CMAC:

CellTracker Blue CMAC Related Antibodies

Top Publications Citing Use of Products

    CellTracker Blue CMAC purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jul 24;16(1):6821.  [Abstract]

    Representativeimages depicting the co-localization the green region (indicative of Ca2+ by Fluo-3AM) in relation to the blue region (marked by Cell Tracker Blue（25 μM，30 min） as vacuoles) wereobtained from ERG11/erg11Δ and erg11m/erg11Δ mutants.

    CellTracker Blue CMAC purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Aug 21:e10068.  [Abstract]

    Mycelia expressing MoPrb1-GFP were stained with CellTracker Blue CMAC (10 µM) and observed using a confocal microscope.

    CellTracker Blue CMAC purchased from MedChemExpress. Usage Cited in: bioRxiv. 2025 Sep 17.

    Colocalization between Erg11-GFP and vacuole in cells precultured at 30 °C or 37 °C for 1 h. Pseudo-color was used to display the blue fluorescence of the vacuole dye (CellTracker Blue CMAC; 100 μM; 30 min) as red. The white arrow indicated vacuoles with Erg11-GFP.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    CellTracker Blue CMAC is a non-fluorescent cell membrane permeable dye. The chloromethyl groups of CellTracker Blue CMAC are enzymatically cleaved by intracellular glutathione (GSH) to generate a fluorescent product (blue fluorescence, Ex/Em: 360/460 nm). CellTracker Blue CMAC is suitable for long-term cell tracking (up to 72 hours) and cell proliferation studies, and can also quantify GSH levels[1][2][3].

    In Vitro

    Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
    1. Dye preparation
    Stock solution: Dissolve in DMSO to a final concentration of 10 mM (protect from light and store in aliquots).
    Working solution: Dilute the stock solution to a working concentration of 0.5-25 μM in serum-free medium. Preheat the staining working solution to 37 °C.
    2. Cell labeling
    Remove the cell culture medium and wash the cells with PBS.
    Add the dye working solution and incubate at 37 °C for 30 minutes.
    Remove the dye and wash 3 times with pre-warmed culture medium.
    Change to fresh culture medium and continue culturing or experiment (fluorescence signal reaches peak value within 1-2 hours).
    3. Detection
    Fluorescence microscopy: (Ex 360 nm, Em 460 nm).
    4. Precautions
    4.1 Please adjust the CellTracker Blue CMAC working solution concentration, incubation temperature and time according to actual conditions.
    4.2 This product is limited to scientific research by professionals and shall not be used for clinical diagnosis or treatment, nor for food or medicine.
    4.3 For your safety and health, please wear a lab coat and disposable gloves when operating.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    CellTracker Blue CMAC Related Antibodies
    Molecular Weight

    209.63

    Formula

    C10H8ClNO2
    CAS No.
    Appearance

    Solid

    Color

    White to light yellow

    Emission (Em)

    470
    Excitation (Ex)

    372
    SMILES

    O=C1OC2=C(C(CCl)=C1)C=CC(N)=C2
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 125 mg/mL (596.29 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 4.7703 mL 23.8515 mL 47.7031 mL
    5 mM 0.9541 mL 4.7703 mL 9.5406 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 4.7703 mL 23.8515 mL 47.7031 mL 119.2577 mL
    5 mM 0.9541 mL 4.7703 mL 9.5406 mL 23.8515 mL
    10 mM 0.4770 mL 2.3852 mL 4.7703 mL 11.9258 mL
    15 mM 0.3180 mL 1.5901 mL 3.1802 mL 7.9505 mL
    20 mM 0.2385 mL 1.1926 mL 2.3852 mL 5.9629 mL
    25 mM 0.1908 mL 0.9541 mL 1.9081 mL 4.7703 mL
    30 mM 0.1590 mL 0.7951 mL 1.5901 mL 3.9753 mL
    40 mM 0.1193 mL 0.5963 mL 1.1926 mL 2.9814 mL
    50 mM 0.0954 mL 0.4770 mL 0.9541 mL 2.3852 mL
    60 mM 0.0795 mL 0.3975 mL 0.7951 mL 1.9876 mL
    80 mM 0.0596 mL 0.2981 mL 0.5963 mL 1.4907 mL
    100 mM 0.0477 mL 0.2385 mL 0.4770 mL 1.1926 mL
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    CellTracker Blue CMAC Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    CellTracker Blue CMAC147963-22-2Fluorescent DyeFluorescent dyeChloromethylCell proteinsCell trackingInhibitorinhibitorinhibit

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