5-Carboxyfluorescein diacetate N-succinimidyl ester
Based on 11 publication(s) in Google Scholar
5-Carboxyfluorescein diacetate N-succinimidyl ester is a cell permeable dye (Ex=492 nm, Em=517 nm). 5-Carboxyfluorescein diacetate N-succinimidyl ester can label cells by covalently binding to intracellular molecules. 5-Carboxyfluorescein diacetate N-succinimidyl ester is used to track lymphocyte migration and proliferation.
For research use only. We do not sell to patients.
- Purity : 99.30%
- CAS No.: 150206-05-6
- Formula: C29H19NO11
- Molecular Weight:557.46
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Storage:
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) 5-Carboxyfluorescein diacetate N-succinimidyl ester
More- J Adv Res. 2025 Aug:74:237-253. [Abstract]
- Cell Rep Med. 2026 Mar 17;7(3):102667. [Abstract]
- Biomaterials. 2023 Nov:302:122297. [Abstract]
- Cell Death Discov. 2023 Nov 28;9(1):427. [Abstract]
- Gels. 2024 Dec 19;10(12):838. [Abstract]
- Int J Pharm. 2024 Jan 5:649:123640. [Abstract]
- PLoS Pathog. 2023 Apr 14;19(4):e1011329. [Abstract]
- Mol Cell Biochem. 2025 Oct 15. [Abstract]
- iScience. 2026 Jul 14;29(8):116793.
- J Cell Commun Signal. 2025 Aug 24;19(3):e70040. [Abstract]
- bioRxiv. 2023 Oct 20.
Biological Activity
Description
In Vitro
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1].
1. Dilute the stock solution in PBS (to give a 50 μM solution).
2. Add 110 μL of this solution per milliliter of cells (to give a final concentration of 5 μM) and mix rapidly.
3. After 5 min at room temperature, add 10 vol of PBS containing 5% FBS.
4. Centrifuge cells 5 min at 300 ×g, 20 °C.
5. Remove the supernatant, and wash threetimes, each time by resuspending in 10 vol PBS containing 5% FBS.
6. Centrifuge cells 5 min at 300 × g, 20 °C, and remove the supernatant.
Note: Labeling occurs rapidly, and it is essential that the tracker is dispersed as evenly and quickly as possible so that cells are uniformly labeled.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Emission (Em)
525
Excitation (Ex)
482
Chemical Information
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CAS No. 150206-05-6
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Appearance Solid
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Molecular Weight 557.46
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Formula C29H19NO11
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Color White to off-white
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SMILES
O=C(C1=CC2=C(C3(C4=C(OC5=C3C=CC(OC(C)=O)=C5)C=C(OC(C)=O)C=C4)OC2=O)C=C1)ON6C(CCC6=O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (11)
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Journal Impact Factor
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Most Recent
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J Adv Res
The fatty acid receptor CD36 promotes macrophage infiltration via p110γ signaling to stimulate metastasis. [Abstract]2025 Aug:74:237-253. PMID: 39419288 -
Cell Rep Med
Engineering lipid nanoparticle-stabilized emulsions for spatiotemporal mRNA delivery and enhanced T cell immunity. [Abstract]2026 Mar 17;7(3):102667. PMID: 41850240 -
Biomaterials
Heteroantigen-assembled nanovaccine enhances the polyfunctionality of TILs against tumor growth and metastasis. [Abstract]2023 Nov:302:122297. PMID: 37666102 -
Cell Death Discov
2023 Nov 28;9(1):427. PMID: 38016969 -
Gels
Whole-Cell Vaccine Preparation Through Prussian Blue Nanoparticles-Elicited Immunogenic Cell Death and Loading in Gel Microneedles Patches. [Abstract]2024 Dec 19;10(12):838. PMID: 39727596 -
Int J Pharm
Enhancing Osteosarcoma Therapy through Aluminium Hydroxide Nanosheets-Enabled Macrophage Modulation. [Abstract]2024 Jan 5:649:123640. PMID: 38043749 -
PLoS Pathog
Tim-3 regulates the immunosuppressive function of decidual MDSCs via the Fyn-STAT3-C/EBPβ pathway during Toxoplasma gondii infection. [Abstract]2023 Apr 14;19(4):e1011329. PMID: 37058540 -
Mol Cell Biochem
Inhibition of LSD1 enhances T cells anti-tumor immunity by promoting TNFSF14 expression in gastric cancer. [Abstract]2025 Oct 15. PMID: 41091427 -
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J Cell Commun Signal
CD2-CD58 axis orchestrates cytotoxic T lymphocyte function and metabolic crosstalk in breast cancer brain metastasis. [Abstract]2025 Aug 24;19(3):e70040. PMID: 40859981 -
Solvent & Solubility
In Vitro:
DMSO : 20.83 mg/mL (37.37 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (280 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.7939 mL | 8.9693 mL | 17.9385 mL | 44.8463 mL |
| 5 mM | 0.3588 mL | 1.7939 mL | 3.5877 mL | 8.9693 mL | |
| 10 mM | 0.1794 mL | 0.8969 mL | 1.7939 mL | 4.4846 mL | |
| 15 mM | 0.1196 mL | 0.5980 mL | 1.1959 mL | 2.9898 mL | |
| 20 mM | 0.0897 mL | 0.4485 mL | 0.8969 mL | 2.2423 mL | |
| 25 mM | 0.0718 mL | 0.3588 mL | 0.7175 mL | 1.7939 mL | |
| 30 mM | 0.0598 mL | 0.2990 mL | 0.5980 mL | 1.4949 mL |
5-Carboxyfluorescein diacetate N-succinimidyl ester Related Classifications
HY-D0056 Related Classifications
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